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The map label for this gene is fhlA [H]

Identifier: 209396082

GI number: 209396082

Start: 3682467

End: 3684545

Strand: Direct

Name: fhlA [H]

Synonym: ECH74115_3983

Alternate gene names: 209396082

Gene position: 3682467-3684545 (Clockwise)

Preceding gene: 209396976

Following gene: 209399182

Centisome position: 66.09

GC content: 52.19

Gene sequence:

>2079_bases
ATGTCATATACACCGATGAGTGATCTCGGACAGCAAGGGTTGTTCGACATCACTCGGACACTATTGCAGCAGCCCGATCT
GGCCTCGCTGTGTGAGGCTCTTTCGCAACTGGTAAAGCGTTCTGCGCTCGCCGACAACGCGGCTATTGTGTTGTGGCAAG
CGCAGACTCAACGTGCGTCTTATTACGCATCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCA
CACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGTTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCA
GCTGGCCGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTG
GCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTT
TCTGTCGTCACCGAACAAATCCAGAGTCGCGTCGTTAACAATGTCGACTATGAGTTGTTATGCCGGGAACGCGATAACTT
CCGCATCCTGGTCGCCATCACCAACGCGGTGCTTTCCCGCCTGGATATGGACGAACTGGTCAGCGAAGTCGCCAAAGAAA
TCCATTACTATTTCGATATTGACGATATCAGTATCGTCTTACGCAGCCACCGTAAAAACAAACTCAACATCTACTCCACT
CACTATCTTGATAAACAGCATCCCGCCCACGAACAGAGCGAAGTCGATGAAGCCGGAACCCTCACCGAACGCGTGTTCAA
AAGTAAAGAGATGCTGCTGATTAATCTCCACGAGCGGGATGATTTAGCCCCCTATGAACGCATGTTGTTCGATACCTGGG
GCAACCAGATTCAAACCTTGTGCCTGTTACCGCTGATGTCTGGCGACACCATGCTGGGCGTGCTGAAACTGGCGCAATGT
GAAGAGAAAGTGTTTACCACTACCAATCTGAATTTACTGCGCCAGATTGCCGAACGTGTGGCAATCGCTGTCGATAACGC
CCTCGCCTATCAGGAAATCCATCGTCTGAAAGAACGGCTGGTTGATGAAAACCTCGCCCTGACCGAGCAGCTCAACAATG
TTGATAGTGAATTTGGCGAGATTATTGGCCGCAGCGAAGCCATGTACAGCGTGCTTAAACAAGTTGAAATGGTGGCGCAA
AGTGACAGTACCGTGCTGATCCTCGGTGAAACTGGCACGGGTAAAGAGCTGATTGCCCGTGCTATCCATAATCTCAGTGG
GCGTAATAATCGCCGCATGGTCAAAATGAACTGCGCGGCGATGCCTGCCGGATTGCTGGAGAGCGATCTGTTTGGTCATG
AGCGTGGGGCTTTTACCGGTGCCAGCGCCCAGCGTATCGGTCGTTTTGAACTGGCGGATAAAAGCTCCCTGTTCCTCGAC
GAAGTGGGCGATATGCCACTGGAGTTACAGCCGAAGTTGCTGCGTGTATTGCAGGAACAGGAGTTTGAACGCCTCGGCAG
CAACAAAATCATTCAGACGGACGTGCGTCTAATCGCCGCGACTAACCGCGATCTGAAAAAAATGGTCGCCGACCGTGAGT
TCCGTAGCGATCTCTATTACCGCCTGAACGTATTCCCGATTCACCTGCCGCCACTACGCGAGCGTCCGGAAGATATTCCG
CTGCTGGCGAAAGCCTTTACCTTCAAAATTGCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGTTGCG
CACCTTGAGCAATATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAACGTCATTGAGCGCGCGGTATTGCTAACACGCG
GCAACGTGCTGCAGCTGTCATTGCCAGATATTGCTTTACCGGAGCCTGAAACGCCGCCTGCCGCAACGGTTGTCGCTCAG
GAGGGCGAAGATGAATATCAGTTGATTGTTCGCGTGCTGAAAGAAACTAACGGCGTGGTTGCCGGGCCTAAAGGCGCTGC
GCAACGTCTGGGGCTGAAACGCACGACCCTGCTGTCACGGATGAAGCGACTGGGAATTGATAAATCGGCATTGATTTAA

Upstream 100 bases:

>100_bases
GCCGAACCGCTTCCGCGTATATGCTAATAAAATTCTAAATCTCCTATAGTTAGTCAATGACCTTTTGCACCGCTTTGCGG
TGCTTTCCTGGAAGAACAAA

Downstream 100 bases:

>100_bases
CTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATACTATTCACGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTG
GCACCGGACACTTGTCATCG

Product: formate hydrogenlyase transcriptional activator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 692; Mature: 691

Protein sequence:

>692_residues
MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALADNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLA
HGPVRSILSRPDTLHCSYEEFCETWPQLAAGGLYPKFGHYCLMPLAAEGHIFGGCEFIRYDDRPWSEKEFNRLQTFTQIV
SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYFDIDDISIVLRSHRKNKLNIYST
HYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINLHERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQC
EEKVFTTTNLNLLRQIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVLKQVEMVAQ
SDSTVLILGETGTGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDLFGHERGAFTGASAQRIGRFELADKSSLFLD
EVGDMPLELQPKLLRVLQEQEFERLGSNKIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIP
LLAKAFTFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDIALPEPETPPAATVVAQ
EGEDEYQLIVRVLKETNGVVAGPKGAAQRLGLKRTTLLSRMKRLGIDKSALI

Sequences:

>Translated_692_residues
MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALADNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLA
HGPVRSILSRPDTLHCSYEEFCETWPQLAAGGLYPKFGHYCLMPLAAEGHIFGGCEFIRYDDRPWSEKEFNRLQTFTQIV
SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYFDIDDISIVLRSHRKNKLNIYST
HYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINLHERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQC
EEKVFTTTNLNLLRQIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVLKQVEMVAQ
SDSTVLILGETGTGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDLFGHERGAFTGASAQRIGRFELADKSSLFLD
EVGDMPLELQPKLLRVLQEQEFERLGSNKIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIP
LLAKAFTFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDIALPEPETPPAATVVAQ
EGEDEYQLIVRVLKETNGVVAGPKGAAQRLGLKRTTLLSRMKRLGIDKSALI
>Mature_691_residues
SYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALADNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAH
GPVRSILSRPDTLHCSYEEFCETWPQLAAGGLYPKFGHYCLMPLAAEGHIFGGCEFIRYDDRPWSEKEFNRLQTFTQIVS
VVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYFDIDDISIVLRSHRKNKLNIYSTH
YLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINLHERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCE
EKVFTTTNLNLLRQIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVLKQVEMVAQS
DSTVLILGETGTGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDLFGHERGAFTGASAQRIGRFELADKSSLFLDE
VGDMPLELQPKLLRVLQEQEFERLGSNKIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPL
LAKAFTFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDIALPEPETPPAATVVAQE
GEDEYQLIVRVLKETNGVVAGPKGAAQRLGLKRTTLLSRMKRLGIDKSALI

Specific function: Required for induction of expression of the formate dehydrogenase H and hydrogenase-3 structural genes [H]

COG id: COG3604

COG function: function code KT; Transcriptional regulator containing GAF, AAA-type ATPase, and DNA binding domains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1789087, Length=692, Percent_Identity=99.5664739884393, Blast_Score=1419, Evalue=0.0,
Organism=Escherichia coli, GI87082117, Length=566, Percent_Identity=51.0600706713781, Blast_Score=522, Evalue=1e-149,
Organism=Escherichia coli, GI1790437, Length=313, Percent_Identity=46.9648562300319, Blast_Score=271, Evalue=1e-73,
Organism=Escherichia coli, GI1788550, Length=322, Percent_Identity=46.2732919254658, Blast_Score=269, Evalue=4e-73,
Organism=Escherichia coli, GI1788905, Length=307, Percent_Identity=46.9055374592834, Blast_Score=254, Evalue=9e-69,
Organism=Escherichia coli, GI87082152, Length=366, Percent_Identity=39.344262295082, Blast_Score=244, Evalue=2e-65,
Organism=Escherichia coli, GI1790299, Length=365, Percent_Identity=41.0958904109589, Blast_Score=241, Evalue=2e-64,
Organism=Escherichia coli, GI1789233, Length=244, Percent_Identity=47.5409836065574, Blast_Score=234, Evalue=1e-62,
Organism=Escherichia coli, GI87081872, Length=265, Percent_Identity=45.2830188679245, Blast_Score=212, Evalue=6e-56,
Organism=Escherichia coli, GI1786524, Length=332, Percent_Identity=38.855421686747, Blast_Score=203, Evalue=3e-53,
Organism=Escherichia coli, GI1787583, Length=333, Percent_Identity=36.6366366366366, Blast_Score=189, Evalue=6e-49,
Organism=Escherichia coli, GI1789828, Length=254, Percent_Identity=36.2204724409449, Blast_Score=141, Evalue=1e-34,
Organism=Escherichia coli, GI87081858, Length=326, Percent_Identity=31.9018404907975, Blast_Score=139, Evalue=7e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003018
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078 [H]

Pfam domain/function: PF01590 GAF; PF02954 HTH_8; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 78412; Mature: 78281

Theoretical pI: Translated: 5.40; Mature: 5.40

Prosite motif: PS00675 SIGMA54_INTERACT_1 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALADNAAIVLWQAQTQRAS
CCCCCHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHH
YYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFCETWPQLAAGGLYPKFGHY
HHHCCCCCCCCCCCCCEEEECCCHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCE
CLMPLAAEGHIFGGCEFIRYDDRPWSEKEFNRLQTFTQIVSVVTEQIQSRVVNNVDYELL
EEEEECCCCCEECCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
CRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYFDIDDISIVLRSHRKNKLNIYST
EECCCCCEEEEEEHHHHHHHCCHHHHHHHHHHHHHEEECCHHHHHHHHHCCCCCEEEEEE
HYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINLHERDDLAPYERMLFDTWGNQIQTL
ECCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHH
CLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLRQIAERVAIAVDNALAYQEIHRLKERL
HHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VDENLALTEQLNNVDSEFGEIIGRSEAMYSVLKQVEMVAQSDSTVLILGETGTGKELIAR
HCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHH
AIHNLSGRNNRRMVKMNCAAMPAGLLESDLFGHERGAFTGASAQRIGRFELADKSSLFLD
HHHHCCCCCCCEEEEEECHHCCCHHHHHHHCCCCCCCCCCCHHHHCCCEEECCCCHHHHH
EVGDMPLELQPKLLRVLQEQEFERLGSNKIIQTDVRLIAATNRDLKKMVADREFRSDLYY
HHCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEHHHHEEEECCHHHHHHHHHHHHHHCCEE
RLNVFPIHLPPLRERPEDIPLLAKAFTFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRE
EEEEEEEECCCHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCHHH
LENVIERAVLLTRGNVLQLSLPDIALPEPETPPAATVVAQEGEDEYQLIVRVLKETNGVV
HHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCEE
AGPKGAAQRLGLKRTTLLSRMKRLGIDKSALI
ECCCHHHHHCCCHHHHHHHHHHHCCCCHHHCC
>Mature Secondary Structure 
SYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALADNAAIVLWQAQTQRAS
CCCCHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHH
YYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFCETWPQLAAGGLYPKFGHY
HHHCCCCCCCCCCCCCEEEECCCHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCE
CLMPLAAEGHIFGGCEFIRYDDRPWSEKEFNRLQTFTQIVSVVTEQIQSRVVNNVDYELL
EEEEECCCCCEECCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
CRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYFDIDDISIVLRSHRKNKLNIYST
EECCCCCEEEEEEHHHHHHHCCHHHHHHHHHHHHHEEECCHHHHHHHHHCCCCCEEEEEE
HYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINLHERDDLAPYERMLFDTWGNQIQTL
ECCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHH
CLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLRQIAERVAIAVDNALAYQEIHRLKERL
HHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VDENLALTEQLNNVDSEFGEIIGRSEAMYSVLKQVEMVAQSDSTVLILGETGTGKELIAR
HCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHH
AIHNLSGRNNRRMVKMNCAAMPAGLLESDLFGHERGAFTGASAQRIGRFELADKSSLFLD
HHHHCCCCCCCEEEEEECHHCCCHHHHHHHCCCCCCCCCCCHHHHCCCEEECCCCHHHHH
EVGDMPLELQPKLLRVLQEQEFERLGSNKIIQTDVRLIAATNRDLKKMVADREFRSDLYY
HHCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEHHHHEEEECCHHHHHHHHHHHHHHCCEE
RLNVFPIHLPPLRERPEDIPLLAKAFTFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRE
EEEEEEEECCCHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCHHH
LENVIERAVLLTRGNVLQLSLPDIALPEPETPPAATVVAQEGEDEYQLIVRVLKETNGVV
HHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCEE
AGPKGAAQRLGLKRTTLLSRMKRLGIDKSALI
ECCCHHHHHCCCHHHHHHHHHHHCCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2280686; 2118503; 9278503 [H]