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The map label for this gene is gidB

Identifier: 20809123

GI number: 20809123

Start: 2683242

End: 2683961

Strand: Reverse

Name: gidB

Synonym: TTE2794

Alternate gene names: 20809123

Gene position: 2683961-2683242 (Counterclockwise)

Preceding gene: 20809124

Following gene: 20809122

Centisome position: 99.8

GC content: 35.56

Gene sequence:

>720_bases
ATGAAGCAAAAATCAATTGAAATGCTAGTACAAGGAGCGAGTAGTTTAGGGATTACTTTGGAAATGTTCCATGTGGAACA
TTTCCAAAAATTTTATTCTCTTTTATTGGAATGGAATCAAAAGATGAATTTAACGGCAATTACTGAGGAAGAGGAAGTTG
TGATAAAGCATTTTTTGGACAGCCTTTCTGTTATAAAAAGCGGCAAAATAAAAGGGGGAGAAAAAGTTATAGATATAGGA
ACAGGGGCTGGATTTCCAGGGATTCCTTTAAAAATAGTTTTTCCTGAAATTGGACTTACTCTTCTTGAAGCATCCAAAAA
GAAAGTAAATTTTTTAAATTTCCTTTCTCAAGTTCTTCTTTTTGAGGGTATTGAAGTAATACACGGAAGAGCAGAAGAAC
TGGGAAAAGTTGAAAGGTTTAGAGAAAAATTTGATATTGCTACTGCAAGAGCCGTGGCTCCTCTCAATATTTTACTGGAA
TATGCTGTTCCTTTTGTGAGGGTGGGAGGACATTTTATTGCGATGAAGGGAAGAGATATAGAAGAAGTATATCAGTGCAA
GAATGCTTTAGAAGAGCTTAAATGCGAGATTGAAGATGTGATTGAAGTGAGGCTTCCTTTTAGTGATATTCTTCACCATT
TGATTGTAGTGAAAAAGGTTGATGTCCTGCCTTCAAAGTATCCTCGAAGGGAAAAAGCCATAAGGACAAAACCTTTGTAA

Upstream 100 bases:

>100_bases
CCTACTTCTGTTGGACAGGCATCCCGTATTTCAGGGGTTTCTCCTGCTGATATTTCTGTGCTTTTAATATACCTACAACA
GATGAGGAGAAAGAGGTCCG

Downstream 100 bases:

>100_bases
TATAGGCGCTTACCTTTTGTGGGCGCTCAGTTTGTTGACAAAGTCTTTCTTATTTAAGTGGCATTTTGTGTTAATGCTCC
AGAGTTGCAAAGCGACTAAA

Product: 16S rRNA methyltransferase GidB

Products: NA

Alternate protein names: 16S rRNA 7-methylguanosine methyltransferase; 16S rRNA m7G methyltransferase

Number of amino acids: Translated: 239; Mature: 239

Protein sequence:

>239_residues
MKQKSIEMLVQGASSLGITLEMFHVEHFQKFYSLLLEWNQKMNLTAITEEEEVVIKHFLDSLSVIKSGKIKGGEKVIDIG
TGAGFPGIPLKIVFPEIGLTLLEASKKKVNFLNFLSQVLLFEGIEVIHGRAEELGKVERFREKFDIATARAVAPLNILLE
YAVPFVRVGGHFIAMKGRDIEEVYQCKNALEELKCEIEDVIEVRLPFSDILHHLIVVKKVDVLPSKYPRREKAIRTKPL

Sequences:

>Translated_239_residues
MKQKSIEMLVQGASSLGITLEMFHVEHFQKFYSLLLEWNQKMNLTAITEEEEVVIKHFLDSLSVIKSGKIKGGEKVIDIG
TGAGFPGIPLKIVFPEIGLTLLEASKKKVNFLNFLSQVLLFEGIEVIHGRAEELGKVERFREKFDIATARAVAPLNILLE
YAVPFVRVGGHFIAMKGRDIEEVYQCKNALEELKCEIEDVIEVRLPFSDILHHLIVVKKVDVLPSKYPRREKAIRTKPL
>Mature_239_residues
MKQKSIEMLVQGASSLGITLEMFHVEHFQKFYSLLLEWNQKMNLTAITEEEEVVIKHFLDSLSVIKSGKIKGGEKVIDIG
TGAGFPGIPLKIVFPEIGLTLLEASKKKVNFLNFLSQVLLFEGIEVIHGRAEELGKVERFREKFDIATARAVAPLNILLE
YAVPFVRVGGHFIAMKGRDIEEVYQCKNALEELKCEIEDVIEVRLPFSDILHHLIVVKKVDVLPSKYPRREKAIRTKPL

Specific function: Specifically methylates the N7 position of a guanosine in 16S rRNA

COG id: COG0357

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in bacterial cell division

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA methyltransferase rsmG family

Homologues:

Organism=Escherichia coli, GI1790179, Length=202, Percent_Identity=34.1584158415842, Blast_Score=111, Evalue=5e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMG_THETN (Q8R6L0)

Other databases:

- EMBL:   AE008691
- RefSeq:   NP_624294.1
- ProteinModelPortal:   Q8R6L0
- SMR:   Q8R6L0
- GeneID:   995856
- GenomeReviews:   AE008691_GR
- KEGG:   tte:TTE2794
- NMPDR:   fig|273068.3.peg.2375
- HOGENOM:   HBG686577
- OMA:   AITHPNS
- ProtClustDB:   PRK00107
- BioCyc:   TTEN273068:TTE2794-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00074
- InterPro:   IPR003682
- PIRSF:   PIRSF003078
- TIGRFAMs:   TIGR00138

Pfam domain/function: PF02527 GidB

EC number: 2.1.-.-

Molecular weight: Translated: 27081; Mature: 27081

Theoretical pI: Translated: 7.70; Mature: 7.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQKSIEMLVQGASSLGITLEMFHVEHFQKFYSLLLEWNQKMNLTAITEEEEVVIKHFLD
CCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHH
SLSVIKSGKIKGGEKVIDIGTGAGFPGIPLKIVFPEIGLTLLEASKKKVNFLNFLSQVLL
HHHHHHCCCCCCCCEEEEECCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHH
FEGIEVIHGRAEELGKVERFREKFDIATARAVAPLNILLEYAVPFVRVGGHFIAMKGRDI
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCH
EEVYQCKNALEELKCEIEDVIEVRLPFSDILHHLIVVKKVDVLPSKYPRREKAIRTKPL
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCC
>Mature Secondary Structure
MKQKSIEMLVQGASSLGITLEMFHVEHFQKFYSLLLEWNQKMNLTAITEEEEVVIKHFLD
CCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHH
SLSVIKSGKIKGGEKVIDIGTGAGFPGIPLKIVFPEIGLTLLEASKKKVNFLNFLSQVLL
HHHHHHCCCCCCCCEEEEECCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHH
FEGIEVIHGRAEELGKVERFREKFDIATARAVAPLNILLEYAVPFVRVGGHFIAMKGRDI
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCH
EEVYQCKNALEELKCEIEDVIEVRLPFSDILHHLIVVKKVDVLPSKYPRREKAIRTKPL
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11997336