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The map label for this gene is rlmH2

Identifier: 20809025

GI number: 20809025

Start: 2577143

End: 2577589

Strand: Reverse

Name: rlmH2

Synonym: TTE2681

Alternate gene names: 20809025

Gene position: 2577589-2577143 (Counterclockwise)

Preceding gene: 20809026

Following gene: 20809023

Centisome position: 95.84

GC content: 33.78

Gene sequence:

>447_bases
ATGAAAATACAAATAATAGCTGTTGGAACTATTAAGGAAAATTATATAAAAGATGGAATAAAATTTTATCTGGACAAGCT
GAAGAAAATATATGATATTGAAATTGTTGAAATAAAAGAAGAAGAGGACCCTGGTACTCCAGCAAGTAGAGAAAAGGTAT
TAGAGACAGAAGGAGAAAGGATTTTATACAAAATTGACAGGGAAAGCTTTGTGATAGCTCTTGCCATAGAAGGGAAAGAA
GTTTCAACCGAAGAATTTGCAAGCCTTGTAAAAAAAGCTTTTGGTATGGGATACAAAAAAGTAGTCTTTGTAATAGGCGG
TTCACTGGGCATATGGGGAAAAGTCAAAGAAAGGGCTGATGTAAACATCTCTTTTTCTAAGATGACTTTTCCTCATCAGC
TCACAAGGCTTTTGCTTTTAGAGCAAATTTATTATGCTCTTTTGTAG

Upstream 100 bases:

>100_bases
AACTAGATGAGGTTTCTTTTACCGATTGGCTTGCTCCCCACAAATGTGATTTTTGCGATGAACCTCCTAAATATCTGGTG
GTCTAAAGTGGGGCAAGAGG

Downstream 100 bases:

>100_bases
TTTATTTGTGGTATGGTTCATTCCTCAATATTTTATAAGCGCGGTAGAGCTGTTCTGCGAGGAGCGTAGCAATTAATCCC
TTTGACATTTTCATTTGCGA

Product: rRNA large subunit methyltransferase

Products: NA

Alternate protein names: 23S rRNA m3Psi1915 methyltransferase 2; rRNA (pseudouridine-N3-)-methyltransferase rlmH 2

Number of amino acids: Translated: 148; Mature: 148

Protein sequence:

>148_residues
MKIQIIAVGTIKENYIKDGIKFYLDKLKKIYDIEIVEIKEEEDPGTPASREKVLETEGERILYKIDRESFVIALAIEGKE
VSTEEFASLVKKAFGMGYKKVVFVIGGSLGIWGKVKERADVNISFSKMTFPHQLTRLLLLEQIYYALL

Sequences:

>Translated_148_residues
MKIQIIAVGTIKENYIKDGIKFYLDKLKKIYDIEIVEIKEEEDPGTPASREKVLETEGERILYKIDRESFVIALAIEGKE
VSTEEFASLVKKAFGMGYKKVVFVIGGSLGIWGKVKERADVNISFSKMTFPHQLTRLLLLEQIYYALL
>Mature_148_residues
MKIQIIAVGTIKENYIKDGIKFYLDKLKKIYDIEIVEIKEEEDPGTPASREKVLETEGERILYKIDRESFVIALAIEGKE
VSTEEFASLVKKAFGMGYKKVVFVIGGSLGIWGKVKERADVNISFSKMTFPHQLTRLLLLEQIYYALL

Specific function: Specifically methylates the pseudouridine at position 1915 (m3Psi1915) in 23S rRNA

COG id: COG1576

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase rlmH family

Homologues:

Organism=Escherichia coli, GI1786855, Length=146, Percent_Identity=30.8219178082192, Blast_Score=76, Evalue=8e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMH2_THETN (Q8R6V3)

Other databases:

- EMBL:   AE008691
- RefSeq:   NP_624196.1
- ProteinModelPortal:   Q8R6V3
- SMR:   Q8R6V3
- GeneID:   997764
- GenomeReviews:   AE008691_GR
- KEGG:   tte:TTE2681
- NMPDR:   fig|273068.3.peg.2217
- HOGENOM:   HBG622807
- OMA:   WIREGIN
- ProtClustDB:   PRK00103
- BioCyc:   TTEN273068:TTE2681-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00658
- InterPro:   IPR003742
- PIRSF:   PIRSF004505

Pfam domain/function: PF02590 SPOUT_MTase

EC number: NA

Molecular weight: Translated: 16909; Mature: 16909

Theoretical pI: Translated: 7.50; Mature: 7.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIQIIAVGTIKENYIKDGIKFYLDKLKKIYDIEIVEIKEEEDPGTPASREKVLETEGER
CEEEEEEEECCHHHHHHHHHHHHHHHHHHHHCEEEEEEECCCCCCCCCHHHHHHHCCCCE
ILYKIDRESFVIALAIEGKEVSTEEFASLVKKAFGMGYKKVVFVIGGSLGIWGKVKERAD
EEEEECCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCC
VNISFSKMTFPHQLTRLLLLEQIYYALL
CEEEEHHCCCHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure
MKIQIIAVGTIKENYIKDGIKFYLDKLKKIYDIEIVEIKEEEDPGTPASREKVLETEGER
CEEEEEEEECCHHHHHHHHHHHHHHHHHHHHCEEEEEEECCCCCCCCCHHHHHHHCCCCE
ILYKIDRESFVIALAIEGKEVSTEEFASLVKKAFGMGYKKVVFVIGGSLGIWGKVKERAD
EEEEECCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCC
VNISFSKMTFPHQLTRLLLLEQIYYALL
CEEEEHHCCCHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11997336