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The map label for this gene is TyrS

Identifier: 20809023

GI number: 20809023

Start: 2575412

End: 2576632

Strand: Reverse

Name: TyrS

Synonym: TTE2679

Alternate gene names: 20809023

Gene position: 2576632-2575412 (Counterclockwise)

Preceding gene: 20809025

Following gene: 20809022

Centisome position: 95.81

GC content: 41.85

Gene sequence:

>1221_bases
GTGTCGGTGCTAGAGGTTTTAAAAGAAAGAGGATATATCGCTCAAATGACCCATGAAGAAGAGATAGAGAAGCTTTTAGA
AAAAGAAAAGATAACTTTTTACATAGGTTTTGACCCCACTGCTGACAGCCTGCACGTAGGTCATTTGATTCAGATAATGA
CAATGGCTCACATGCAGAGAGCCGGCCACAGGCCAATTGTGCTTTTGGGAGGCGGAACTGCCCTTATAGGAGATCCGAGC
GGTAGAACTGACATGAGAAAAATGCTCACTAAAGAGGAAATAGATAGAAATGCTGAAGCCTTCAAAAAGCAGATGGAGAG
ATTCATCGATTTTTCTGAGGGCAAAGCTATAATGGAAAACAATGCTAAATGGCTCCTTGGCTTGAATTACATAGAATTTT
TGAGAGATATAGGTGTTCACTTTACTGTAAATCGAATGCTTGAGGCGGAAGCTTTTAAGACTCGCATGGAAAGAGGCCTT
ACTTTCCTTGAGTTTAATTACATGCTAATGCAGGCTTATGACTTTTTAGAGCTTTACAGGCGCTATGGCTGTGTAATGCA
GATGGGAGGAAATGATCAGTGGTCTAATATAATCGCTGGAGTAGAGCTTATACGCAAAAAAGAAGGCAAGCAGGCTTATG
GAATGACTTTTGTGCTCCTTACAACAAGCGAAGGAAAGAAGATGGGCAAAACTGAAAAAGGCGCTATATGGCTGGATCCT
AAAAAGACTTCTCCTTATGAATTTTATCAGTACTGGAGAAATATAGGAGACGCAGATGTGGAAAAGGCTTTGGCTCTTTT
GACTTTCCTTCCTATGGACGAAGTGAGAAGGCTTGGGAGACTAAGGGATAAGGAGATAAACGAAGCTAAAAAGGTACTGG
CTTTTGAGGTTACAAAGCTTGTACACGGTGAAGAAGAAGCCCTAAAGGCTCAGAAGGCGGCCGAAGCTCTGTTTGAAGGC
GGCGGTGAAATGGAACACGTGCCCAGCATTGAAGTCTCTCAGGACATAATCGGAAGGAAAATAGTTGATGTGCTTTTTGA
AGCTAAAGTCATACCTTCAAAGAGCGAGGGAAGAAGGCTTATTCAGCAGGGAGGCCTTTACATCAACGACAAGAGGGTAG
AAAACGTGGATGAATGCATAAAAGAAGAGATGGTAAAAGAAAATGCCATTCTTGTAAGAAAAGGGAAAAAGGAATATCAC
AGGTTATTGGTAAAGGAATGA

Upstream 100 bases:

>100_bases
CCTGCTTTTTCATTATATCCTTATTATATCAAAAAAACATTTGAAAATATTTTTTCACTTTGATACAATAATACAAAAAC
CAACTGATGGGGGTATAAAC

Downstream 100 bases:

>100_bases
ATGCAATATGATTTTCCTTTTTACTGCTCTTTATGCTGAAGCTTACCCTTTTATAAACTATTTTAAACTAAAAAGAGACC
TTCAAAACAATTATTTTCAA

Product: tyrosyl-tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase; TyrRS

Number of amino acids: Translated: 406; Mature: 405

Protein sequence:

>406_residues
MSVLEVLKERGYIAQMTHEEEIEKLLEKEKITFYIGFDPTADSLHVGHLIQIMTMAHMQRAGHRPIVLLGGGTALIGDPS
GRTDMRKMLTKEEIDRNAEAFKKQMERFIDFSEGKAIMENNAKWLLGLNYIEFLRDIGVHFTVNRMLEAEAFKTRMERGL
TFLEFNYMLMQAYDFLELYRRYGCVMQMGGNDQWSNIIAGVELIRKKEGKQAYGMTFVLLTTSEGKKMGKTEKGAIWLDP
KKTSPYEFYQYWRNIGDADVEKALALLTFLPMDEVRRLGRLRDKEINEAKKVLAFEVTKLVHGEEEALKAQKAAEALFEG
GGEMEHVPSIEVSQDIIGRKIVDVLFEAKVIPSKSEGRRLIQQGGLYINDKRVENVDECIKEEMVKENAILVRKGKKEYH
RLLVKE

Sequences:

>Translated_406_residues
MSVLEVLKERGYIAQMTHEEEIEKLLEKEKITFYIGFDPTADSLHVGHLIQIMTMAHMQRAGHRPIVLLGGGTALIGDPS
GRTDMRKMLTKEEIDRNAEAFKKQMERFIDFSEGKAIMENNAKWLLGLNYIEFLRDIGVHFTVNRMLEAEAFKTRMERGL
TFLEFNYMLMQAYDFLELYRRYGCVMQMGGNDQWSNIIAGVELIRKKEGKQAYGMTFVLLTTSEGKKMGKTEKGAIWLDP
KKTSPYEFYQYWRNIGDADVEKALALLTFLPMDEVRRLGRLRDKEINEAKKVLAFEVTKLVHGEEEALKAQKAAEALFEG
GGEMEHVPSIEVSQDIIGRKIVDVLFEAKVIPSKSEGRRLIQQGGLYINDKRVENVDECIKEEMVKENAILVRKGKKEYH
RLLVKE
>Mature_405_residues
SVLEVLKERGYIAQMTHEEEIEKLLEKEKITFYIGFDPTADSLHVGHLIQIMTMAHMQRAGHRPIVLLGGGTALIGDPSG
RTDMRKMLTKEEIDRNAEAFKKQMERFIDFSEGKAIMENNAKWLLGLNYIEFLRDIGVHFTVNRMLEAEAFKTRMERGLT
FLEFNYMLMQAYDFLELYRRYGCVMQMGGNDQWSNIIAGVELIRKKEGKQAYGMTFVLLTTSEGKKMGKTEKGAIWLDPK
KTSPYEFYQYWRNIGDADVEKALALLTFLPMDEVRRLGRLRDKEINEAKKVLAFEVTKLVHGEEEALKAQKAAEALFEGG
GEMEHVPSIEVSQDIIGRKIVDVLFEAKVIPSKSEGRRLIQQGGLYINDKRVENVDECIKEEMVKENAILVRKGKKEYHR
LLVKE

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr)

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain

Homologues:

Organism=Homo sapiens, GI94681057, Length=433, Percent_Identity=34.8729792147806, Blast_Score=254, Evalue=1e-67,
Organism=Escherichia coli, GI1787925, Length=424, Percent_Identity=41.2735849056604, Blast_Score=338, Evalue=4e-94,
Organism=Caenorhabditis elegans, GI25145802, Length=388, Percent_Identity=34.2783505154639, Blast_Score=205, Evalue=4e-53,
Organism=Caenorhabditis elegans, GI25145805, Length=307, Percent_Identity=32.8990228013029, Blast_Score=145, Evalue=3e-35,
Organism=Saccharomyces cerevisiae, GI6325160, Length=443, Percent_Identity=30.4740406320542, Blast_Score=231, Evalue=2e-61,
Organism=Drosophila melanogaster, GI20130383, Length=429, Percent_Identity=38.4615384615385, Blast_Score=300, Evalue=1e-81,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYY_THETN (Q8R6V5)

Other databases:

- EMBL:   AE008691
- RefSeq:   NP_624194.1
- ProteinModelPortal:   Q8R6V5
- SMR:   Q8R6V5
- GeneID:   997731
- GenomeReviews:   AE008691_GR
- KEGG:   tte:TTE2679
- NMPDR:   fig|273068.3.peg.2215
- HOGENOM:   HBG288125
- OMA:   TFYIGFD
- ProtClustDB:   PRK05912
- BioCyc:   TTEN273068:TTE2679-MONOMER
- BRENDA:   6.1.1.1
- GO:   GO:0005737
- HAMAP:   MF_02006
- InterPro:   IPR001412
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11766
- PRINTS:   PR01040
- SMART:   SM00363
- TIGRFAMs:   TIGR00234

Pfam domain/function: PF01479 S4; PF00579 tRNA-synt_1b

EC number: =6.1.1.1

Molecular weight: Translated: 46717; Mature: 46586

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: PS00178 AA_TRNA_LIGASE_I; PS50889 S4

Important sites: BINDING 34-34 BINDING 167-167 BINDING 171-171 BINDING 230-230

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVLEVLKERGYIAQMTHEEEIEKLLEKEKITFYIGFDPTADSLHVGHLIQIMTMAHMQR
CCHHHHHHHCCCEEECCCHHHHHHHHHHCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHH
AGHRPIVLLGGGTALIGDPSGRTDMRKMLTKEEIDRNAEAFKKQMERFIDFSEGKAIMEN
CCCCCEEEECCCEEEECCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHC
NAKWLLGLNYIEFLRDIGVHFTVNRMLEAEAFKTRMERGLTFLEFNYMLMQAYDFLELYR
CCCEEEECHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHCCEEEEHHHHHHHHHHHHHHHH
RYGCVMQMGGNDQWSNIIAGVELIRKKEGKQAYGMTFVLLTTSEGKKMGKTEKGAIWLDP
HCCCEEEECCCCHHHHHHHHHHHHHHHCCCHHHCEEEEEEEECCCCCCCCCCCCEEEECC
KKTSPYEFYQYWRNIGDADVEKALALLTFLPMDEVRRLGRLRDKEINEAKKVLAFEVTKL
CCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VHGEEEALKAQKAAEALFEGGGEMEHVPSIEVSQDIIGRKIVDVLFEAKVIPSKSEGRRL
HCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
IQQGGLYINDKRVENVDECIKEEMVKENAILVRKGKKEYHRLLVKE
HHHCCCEEECHHHHHHHHHHHHHHHHCCCEEEECCHHHHHHHHCCC
>Mature Secondary Structure 
SVLEVLKERGYIAQMTHEEEIEKLLEKEKITFYIGFDPTADSLHVGHLIQIMTMAHMQR
CHHHHHHHCCCEEECCCHHHHHHHHHHCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHH
AGHRPIVLLGGGTALIGDPSGRTDMRKMLTKEEIDRNAEAFKKQMERFIDFSEGKAIMEN
CCCCCEEEECCCEEEECCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHC
NAKWLLGLNYIEFLRDIGVHFTVNRMLEAEAFKTRMERGLTFLEFNYMLMQAYDFLELYR
CCCEEEECHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHCCEEEEHHHHHHHHHHHHHHHH
RYGCVMQMGGNDQWSNIIAGVELIRKKEGKQAYGMTFVLLTTSEGKKMGKTEKGAIWLDP
HCCCEEEECCCCHHHHHHHHHHHHHHHCCCHHHCEEEEEEEECCCCCCCCCCCCEEEECC
KKTSPYEFYQYWRNIGDADVEKALALLTFLPMDEVRRLGRLRDKEINEAKKVLAFEVTKL
CCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VHGEEEALKAQKAAEALFEGGGEMEHVPSIEVSQDIIGRKIVDVLFEAKVIPSKSEGRRL
HCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
IQQGGLYINDKRVENVDECIKEEMVKENAILVRKGKKEYHRLLVKE
HHHCCCEEECHHHHHHHHHHHHHHHHCCCEEEECCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11997336