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The map label for this gene is ArgB

Identifier: 20808851

GI number: 20808851

Start: 2385600

End: 2386499

Strand: Reverse

Name: ArgB

Synonym: TTE2496

Alternate gene names: 20808851

Gene position: 2386499-2385600 (Counterclockwise)

Preceding gene: 20808852

Following gene: 20808850

Centisome position: 88.74

GC content: 39.22

Gene sequence:

>900_bases
ATGATAAGGAAAGAAAAATTCGGAGATGAAATTTTAAAAGCACATATCCTTGTGGAGGCGCTCCCCTATATAAAAAAATT
CTCCGGCAAGACAGTTGTGATAAAGTATGGCGGCAGTGCCATGCTGGACTGTAATTTAAAGAAAATGGTTATGCAGGATG
TAGTACTTATGAAATTTGTGGGTTTAAATCCTGTGATAGTTCACGGTGGAGGGCCCGAAATCAGCAGCTTTTTAAAAAAA
CTGGGGATAGAATCAAAGTTTGTAAATGGGCTGAGGGTAACAGATGAAAAAACTGCAGAAATTGTAGAAATGGTTCTTGT
TGGAAAGATAAACAAAGAGATAGTTTCAATGATAAACGAACTGGGAGGCATGGCTGTTGGAATAAGTGGGAAAGACGGAA
AACTTTTAAAAGCAGAAAAAGACCTGTCAAACGGAGACCTGGGGTATGTTGGCAAAATAGTTGAAGTAAACATTGAGGTT
ATAGAGATGCTTATTGATAGAGGCTATATACCTGTTATAGCTCCGGTTTCCTTTGGGGATGATGGAAAGACTTACAATGT
CAATGCCGACACGGCTGCTGGCAAGATAGCAGAAGCCTTAAAGGCGGAAAAATTGATTCTCCTCACAGATGTTGAGGGGG
TTTTAGAAAATGTGGAGGACAAGACGAGTTTGATTTCCAGAATGGACTTGGAACATGCTAAAAAATTGATGGATTCTGGG
CGAATAAACGGGGGAATGATTCCAAAGCTTAAGTGCTGTATAAAAGCTGTAGAAAATGGTGTAAAAAGGGCACACATAAT
AGATGGAAGGCTTACCCATTCTCTTCTTTTAGAAATTTTTACGGATGAGGGGATAGGGACAATGATAGGAAAGGAATGTT
TTGATGATGATAACCTATGA

Upstream 100 bases:

>100_bases
TTTTCATAATAGTTGACATGAAAGCCGGAGAATTTATGGCTACTTCATGGGGATGCGACCTCTCTTACGATTATGTAAAA
ATAAATGGGAGCTACAGGAC

Downstream 100 bases:

>100_bases
AAAGAAATATTTGATGGATACTTACAATAGATATCCTATTATGCTTGTCAAGGGAGAAGGTACAAGAGTATGGGATAGTG
AGGGAAATGCTTACCTCGAC

Product: acetylglutamate kinase

Products: NA

Alternate protein names: N-acetyl-L-glutamate 5-phosphotransferase; NAG kinase; AGK

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MIRKEKFGDEILKAHILVEALPYIKKFSGKTVVIKYGGSAMLDCNLKKMVMQDVVLMKFVGLNPVIVHGGGPEISSFLKK
LGIESKFVNGLRVTDEKTAEIVEMVLVGKINKEIVSMINELGGMAVGISGKDGKLLKAEKDLSNGDLGYVGKIVEVNIEV
IEMLIDRGYIPVIAPVSFGDDGKTYNVNADTAAGKIAEALKAEKLILLTDVEGVLENVEDKTSLISRMDLEHAKKLMDSG
RINGGMIPKLKCCIKAVENGVKRAHIIDGRLTHSLLLEIFTDEGIGTMIGKECFDDDNL

Sequences:

>Translated_299_residues
MIRKEKFGDEILKAHILVEALPYIKKFSGKTVVIKYGGSAMLDCNLKKMVMQDVVLMKFVGLNPVIVHGGGPEISSFLKK
LGIESKFVNGLRVTDEKTAEIVEMVLVGKINKEIVSMINELGGMAVGISGKDGKLLKAEKDLSNGDLGYVGKIVEVNIEV
IEMLIDRGYIPVIAPVSFGDDGKTYNVNADTAAGKIAEALKAEKLILLTDVEGVLENVEDKTSLISRMDLEHAKKLMDSG
RINGGMIPKLKCCIKAVENGVKRAHIIDGRLTHSLLLEIFTDEGIGTMIGKECFDDDNL
>Mature_299_residues
MIRKEKFGDEILKAHILVEALPYIKKFSGKTVVIKYGGSAMLDCNLKKMVMQDVVLMKFVGLNPVIVHGGGPEISSFLKK
LGIESKFVNGLRVTDEKTAEIVEMVLVGKINKEIVSMINELGGMAVGISGKDGKLLKAEKDLSNGDLGYVGKIVEVNIEV
IEMLIDRGYIPVIAPVSFGDDGKTYNVNADTAAGKIAEALKAEKLILLTDVEGVLENVEDKTSLISRMDLEHAKKLMDSG
RINGGMIPKLKCCIKAVENGVKRAHIIDGRLTHSLLLEIFTDEGIGTMIGKECFDDDNL

Specific function: Arginine biosynthesis; first step. [C]

COG id: COG0548

COG function: function code E; Acetylglutamate kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acetylglutamate kinase family

Homologues:

Organism=Escherichia coli, GI1789181, Length=280, Percent_Identity=29.2857142857143, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI145693208, Length=225, Percent_Identity=33.7777777777778, Blast_Score=91, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6320913, Length=274, Percent_Identity=30.2919708029197, Blast_Score=101, Evalue=1e-22,

Paralogues:

None

Copy number: 2,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): ARGB_THETN (Q8R7C0)

Other databases:

- EMBL:   AE008691
- RefSeq:   NP_624022.1
- ProteinModelPortal:   Q8R7C0
- SMR:   Q8R7C0
- GeneID:   995500
- GenomeReviews:   AE008691_GR
- KEGG:   tte:TTE2496
- NMPDR:   fig|273068.3.peg.1894
- HOGENOM:   HBG497643
- OMA:   DGEYEIN
- ProtClustDB:   PRK00942
- BioCyc:   TTEN273068:TTE2496-MONOMER
- BRENDA:   2.7.2.8
- GO:   GO:0005737
- HAMAP:   MF_00082_B
- InterPro:   IPR004662
- InterPro:   IPR001048
- InterPro:   IPR003964
- Gene3D:   G3DSA:3.40.1160.10
- PIRSF:   PIRSF000728
- PRINTS:   PR01469
- TIGRFAMs:   TIGR00761

Pfam domain/function: PF00696 AA_kinase; SSF53633 Aa_kinase

EC number: =2.7.2.8

Molecular weight: Translated: 32518; Mature: 32518

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: NA

Important sites: BINDING 92-92 BINDING 186-186

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRKEKFGDEILKAHILVEALPYIKKFSGKTVVIKYGGSAMLDCNLKKMVMQDVVLMKFV
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCEEEECCHHHHHHHHHHHHHHH
GLNPVIVHGGGPEISSFLKKLGIESKFVNGLRVTDEKTAEIVEMVLVGKINKEIVSMINE
CCCEEEEECCCHHHHHHHHHHCCCHHHHCCEECCCHHHHHHHHHHHHHHCCHHHHHHHHH
LGGMAVGISGKDGKLLKAEKDLSNGDLGYVGKIVEVNIEVIEMLIDRGYIPVIAPVSFGD
HCCEEEEECCCCCCEEEECCCCCCCCCCCCCEEEEEHHHHHHHHHHCCCCEEEEEEEECC
DGKTYNVNADTAAGKIAEALKAEKLILLTDVEGVLENVEDKTSLISRMDLEHAKKLMDSG
CCCEEECCCCHHHHHHHHHHHHCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
RINGGMIPKLKCCIKAVENGVKRAHIIDGRLTHSLLLEIFTDEGIGTMIGKECFDDDNL
CCCCCCCHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHCCCCHHHHHHHHCCCCCC
>Mature Secondary Structure
MIRKEKFGDEILKAHILVEALPYIKKFSGKTVVIKYGGSAMLDCNLKKMVMQDVVLMKFV
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCEEEECCHHHHHHHHHHHHHHH
GLNPVIVHGGGPEISSFLKKLGIESKFVNGLRVTDEKTAEIVEMVLVGKINKEIVSMINE
CCCEEEEECCCHHHHHHHHHHCCCHHHHCCEECCCHHHHHHHHHHHHHHCCHHHHHHHHH
LGGMAVGISGKDGKLLKAEKDLSNGDLGYVGKIVEVNIEVIEMLIDRGYIPVIAPVSFGD
HCCEEEEECCCCCCEEEECCCCCCCCCCCCCEEEEEHHHHHHHHHHCCCCEEEEEEEECC
DGKTYNVNADTAAGKIAEALKAEKLILLTDVEGVLENVEDKTSLISRMDLEHAKKLMDSG
CCCEEECCCCHHHHHHHHHHHHCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
RINGGMIPKLKCCIKAVENGVKRAHIIDGRLTHSLLLEIFTDEGIGTMIGKECFDDDNL
CCCCCCCHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHCCCCHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11997336