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The map label for this gene is AmiC2 [H]

Identifier: 20808785

GI number: 20808785

Start: 2315739

End: 2316398

Strand: Reverse

Name: AmiC2 [H]

Synonym: TTE2424

Alternate gene names: 20808785

Gene position: 2316398-2315739 (Counterclockwise)

Preceding gene: 20808787

Following gene: 20808784

Centisome position: 86.13

GC content: 36.21

Gene sequence:

>660_bases
TTGTTAATAGCTGTAGATCCGGGGCATGGAGGGAAAGACCCTGGGGCTGTTGTAGATGGCTACAAAGAGAAAGACATAAA
TTTAAATGTGGCTTTAAAACTCAGGGATATTCTTACTAAAAACGGCATAGATGTTATAATGACAAGGGATAAGGATAAAA
CAGTAGACCTTCAACAAAGATGCGATATAGCAAACAAAGCTCATGCGAACTATTTCATCTCCATTCACTGCAATAGCTTT
CAAGATCCTTCAGCAAGTGGTACAGAGACTTATGCTTACCCTGGTAGTGTAGATGGGAGAAACTTGGCTCAATATGTCCA
AAATGAAATAGTAAAAATGCTTGGGACAAAAGACAGAGGGGTAAAGTATGAAAAATTTTATGTATTAAAGTACACTGACA
TGCCAGCTATCCTAGTAGAACTTGCCTTTATGTCAAACCCTCATGACCTAAAATTGCTTTTAGAAAAAGATGAACTTTTT
GCAGAAGCTATATCAAGAGGGCTTGAGAATTTCCTAAAAACGACATTTACAGGAGAAGAAATGGCTATACAAAAATTAAA
GATGAAAGGAATTATTGATAAAATTCATGACACGAAAAGTTATGTCACATGGGGCGAGTTTGCTTCTGTAATAATAAAAG
TATTGGAGGGAATGAAATGA

Upstream 100 bases:

>100_bases
AATTTTACAGAATGAAGTAAAAATTATCCATAAAACTTGTAAATTTTTCTTGCATAGTATATCATAGAATAAAAGAGAAA
AATTGAAGGTGGTGGAGAAT

Downstream 100 bases:

>100_bases
AAGAATTGATAATGACTATTTTAACAGCAGGAATACAGCTTCTTGTAATGGTCGTGTTAGGTTATCTTATTGACTATTTG
AGCACAAAAATAGGAATGGA

Product: N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: Cell wall hydrolase; Cell wall-associated polypeptide CWBP49; CWBP49; Major autolysin [H]

Number of amino acids: Translated: 219; Mature: 219

Protein sequence:

>219_residues
MLIAVDPGHGGKDPGAVVDGYKEKDINLNVALKLRDILTKNGIDVIMTRDKDKTVDLQQRCDIANKAHANYFISIHCNSF
QDPSASGTETYAYPGSVDGRNLAQYVQNEIVKMLGTKDRGVKYEKFYVLKYTDMPAILVELAFMSNPHDLKLLLEKDELF
AEAISRGLENFLKTTFTGEEMAIQKLKMKGIIDKIHDTKSYVTWGEFASVIIKVLEGMK

Sequences:

>Translated_219_residues
MLIAVDPGHGGKDPGAVVDGYKEKDINLNVALKLRDILTKNGIDVIMTRDKDKTVDLQQRCDIANKAHANYFISIHCNSF
QDPSASGTETYAYPGSVDGRNLAQYVQNEIVKMLGTKDRGVKYEKFYVLKYTDMPAILVELAFMSNPHDLKLLLEKDELF
AEAISRGLENFLKTTFTGEEMAIQKLKMKGIIDKIHDTKSYVTWGEFASVIIKVLEGMK
>Mature_219_residues
MLIAVDPGHGGKDPGAVVDGYKEKDINLNVALKLRDILTKNGIDVIMTRDKDKTVDLQQRCDIANKAHANYFISIHCNSF
QDPSASGTETYAYPGSVDGRNLAQYVQNEIVKMLGTKDRGVKYEKFYVLKYTDMPAILVELAFMSNPHDLKLLLEKDELF
AEAISRGLENFLKTTFTGEEMAIQKLKMKGIIDKIHDTKSYVTWGEFASVIIKVLEGMK

Specific function: Autolysins are involved in some important biological processes such as cell separation, cell-wall turnover, competence for genetic transformation, formation of the flagella - in particular of its basal body - and sporulation. Has a high affinity for teich

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Secreted, cell wall [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788776, Length=198, Percent_Identity=30.3030303030303, Blast_Score=94, Evalue=6e-21,
Organism=Escherichia coli, GI1790611, Length=225, Percent_Identity=26.6666666666667, Blast_Score=90, Evalue=1e-19,
Organism=Escherichia coli, GI87082163, Length=224, Percent_Identity=31.25, Blast_Score=84, Evalue=7e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007253
- InterPro:   IPR002508 [H]

Pfam domain/function: PF01520 Amidase_3; PF04122 CW_binding_2 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 24570; Mature: 24570

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLIAVDPGHGGKDPGAVVDGYKEKDINLNVALKLRDILTKNGIDVIMTRDKDKTVDLQQR
CEEEECCCCCCCCCCCCCCCCCCCCCCEEEEEEEEHHHHCCCCEEEEECCCCCCCCHHHH
CDIANKAHANYFISIHCNSFQDPSASGTETYAYPGSVDGRNLAQYVQNEIVKMLGTKDRG
CCCCCCCCCEEEEEEEECCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCC
VKYEKFYVLKYTDMPAILVELAFMSNPHDLKLLLEKDELFAEAISRGLENFLKTTFTGEE
CEEEEEEEEEECCCHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHCCCHH
MAIQKLKMKGIIDKIHDTKSYVTWGEFASVIIKVLEGMK
HHHHHHHHHHHHHHHHCCHHHEEHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLIAVDPGHGGKDPGAVVDGYKEKDINLNVALKLRDILTKNGIDVIMTRDKDKTVDLQQR
CEEEECCCCCCCCCCCCCCCCCCCCCCEEEEEEEEHHHHCCCCEEEEECCCCCCCCHHHH
CDIANKAHANYFISIHCNSFQDPSASGTETYAYPGSVDGRNLAQYVQNEIVKMLGTKDRG
CCCCCCCCCEEEEEEEECCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCC
VKYEKFYVLKYTDMPAILVELAFMSNPHDLKLLLEKDELFAEAISRGLENFLKTTFTGEE
CEEEEEEEEEECCCHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHCCCHH
MAIQKLKMKGIIDKIHDTKSYVTWGEFASVIIKVLEGMK
HHHHHHHHHHHHHHHHCCHHHEEHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1357079; 1682302; 9384377 [H]