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The map label for this gene is MesJ3

Identifier: 20808759

GI number: 20808759

Start: 2283780

End: 2285183

Strand: Reverse

Name: MesJ3

Synonym: TTE2395

Alternate gene names: 20808759

Gene position: 2285183-2283780 (Counterclockwise)

Preceding gene: 20808760

Following gene: 20808758

Centisome position: 84.97

GC content: 34.9

Gene sequence:

>1404_bases
GTGACTATGAGTTTTCAGAGGTTTGGTATGATTGAGAAGGTTTTAAGGACCATTGAGAAGTACAATATGATAGAAAAAGA
TGACAAAATTGTAATGGGAATATCAGGAGGCCCCGATTCCCTGTGCATGTTGGATGTACTTTTTAATCTAAAAGGAAAAT
TTAATTTGAAATTGTATGTTGTTCATGTAAACCACATGATAAGAGGAGAAGATGCCAAAAAGGATGCGGAGTTTGTGGAG
AAGCTGTGTAAAGACTTAGATCTTCCCTTTTTTTTATTTGAAGAAAACATACCTTATCTGGCTAAAAAGATGGGATTAAG
CGAAGAGCAAGCTGGGAGATACGTAAGGTACAAGGCTTTTGAGGAAACATTGAAAAGAGTAGGAGGAAACAAAATAGCTG
TAGCCCATAACAAAAATGATGTAGCAGAGACAGTTTTATTAAACATCCTGAGAGGGACGGGGCTTAGGGGACTTATTGGT
ATAAAGCCTGTAAATGGCAATATTATCAGGCCTCTCATTGAGATTGAGAGAAGAGAAATTGAAAAGTATCTGAAGGATAA
AAATTTGCATCCCAGGATTGACCATACCAATTATGAAGATTTGTATACGCGAAATAAGATAAGGCTAAAGGTTATTCCGT
ATATTGAAGAGGTGTTTAAGATAGATTTGGTTGAAAATCTCTCTAGGATGGCGGCGATTTTGTTAGAGGAAGATGATTAT
TTGGAAGCTAAGTGTGAAGAAGTTTTTAATCAAATATGCGAAATAAATGGGGAAGAGATAAAAGTAGATGTAGATGCTTT
AAAGTCACAACATACGGCTATTAAAAGGCGTTTGGTGAGAAGGATGTACTTTTATGTGAAGGGAGAAACTGATGGACTGG
AATATGGGCATGTGGAAGATGTATTAAATCTTTTAGATAAACCTACGTCATCAAAAATTGATTTACCTTTTGAAATTGAG
GCCTTAAAAATGTATAATAATCTTGTAATCAGAAAAAAGAAGACGAAAGAGAAGGTAAAATTTAAAGAGGTGCTAAAGAT
TCCTGGAGTTACAACTATAGAAGGAATTGGCAAATTTAAAGCTTATGTAGTTGATATTTCACAGGTTGATGATTTTAACA
AGGGGGAGTATATAAAATTTTTCGATTATGACAAAATAAAAGGGGAAATAGTTGTAAAAAGCAGAGAAGATGGGGACAGG
TTTTCGCCTCTTGGCATGAGAGGGACGAAAAAATTAAAAGAATTTTTTATTGATGAAAAAATACCCAGAGAAGAGAGGGA
TTACATCCCGCTTGTAGCTATAGGAAAGGAAATTGTGTGGGTTGTAGGTTACAGGATGAGTGAGAAGTTTAAGGTAGACA
AAAATACTTCAAAGGTGCTTGTAATTGAATATACTAAAGAATGA

Upstream 100 bases:

>100_bases
AAATACACCTAAAGACGACATGACAGTGATAGTGGCAAAAATTTGGAAGAAGATTTATGTTTGATTTAAAGCGAGCTATA
GTATATAATCATAGTATAGC

Downstream 100 bases:

>100_bases
AAGAGGAGGTAGTTTTATGCCAAGTCCTATGGAAGACATAGAAGAAATTTTGATAACAGAAGAGCAGTTGAAGGCGAAAG
TTAAAGAACTTGGCGAGATG

Product: ATPase

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase

Number of amino acids: Translated: 467; Mature: 466

Protein sequence:

>467_residues
MTMSFQRFGMIEKVLRTIEKYNMIEKDDKIVMGISGGPDSLCMLDVLFNLKGKFNLKLYVVHVNHMIRGEDAKKDAEFVE
KLCKDLDLPFFLFEENIPYLAKKMGLSEEQAGRYVRYKAFEETLKRVGGNKIAVAHNKNDVAETVLLNILRGTGLRGLIG
IKPVNGNIIRPLIEIERREIEKYLKDKNLHPRIDHTNYEDLYTRNKIRLKVIPYIEEVFKIDLVENLSRMAAILLEEDDY
LEAKCEEVFNQICEINGEEIKVDVDALKSQHTAIKRRLVRRMYFYVKGETDGLEYGHVEDVLNLLDKPTSSKIDLPFEIE
ALKMYNNLVIRKKKTKEKVKFKEVLKIPGVTTIEGIGKFKAYVVDISQVDDFNKGEYIKFFDYDKIKGEIVVKSREDGDR
FSPLGMRGTKKLKEFFIDEKIPREERDYIPLVAIGKEIVWVVGYRMSEKFKVDKNTSKVLVIEYTKE

Sequences:

>Translated_467_residues
MTMSFQRFGMIEKVLRTIEKYNMIEKDDKIVMGISGGPDSLCMLDVLFNLKGKFNLKLYVVHVNHMIRGEDAKKDAEFVE
KLCKDLDLPFFLFEENIPYLAKKMGLSEEQAGRYVRYKAFEETLKRVGGNKIAVAHNKNDVAETVLLNILRGTGLRGLIG
IKPVNGNIIRPLIEIERREIEKYLKDKNLHPRIDHTNYEDLYTRNKIRLKVIPYIEEVFKIDLVENLSRMAAILLEEDDY
LEAKCEEVFNQICEINGEEIKVDVDALKSQHTAIKRRLVRRMYFYVKGETDGLEYGHVEDVLNLLDKPTSSKIDLPFEIE
ALKMYNNLVIRKKKTKEKVKFKEVLKIPGVTTIEGIGKFKAYVVDISQVDDFNKGEYIKFFDYDKIKGEIVVKSREDGDR
FSPLGMRGTKKLKEFFIDEKIPREERDYIPLVAIGKEIVWVVGYRMSEKFKVDKNTSKVLVIEYTKE
>Mature_466_residues
TMSFQRFGMIEKVLRTIEKYNMIEKDDKIVMGISGGPDSLCMLDVLFNLKGKFNLKLYVVHVNHMIRGEDAKKDAEFVEK
LCKDLDLPFFLFEENIPYLAKKMGLSEEQAGRYVRYKAFEETLKRVGGNKIAVAHNKNDVAETVLLNILRGTGLRGLIGI
KPVNGNIIRPLIEIERREIEKYLKDKNLHPRIDHTNYEDLYTRNKIRLKVIPYIEEVFKIDLVENLSRMAAILLEEDDYL
EAKCEEVFNQICEINGEEIKVDVDALKSQHTAIKRRLVRRMYFYVKGETDGLEYGHVEDVLNLLDKPTSSKIDLPFEIEA
LKMYNNLVIRKKKTKEKVKFKEVLKIPGVTTIEGIGKFKAYVVDISQVDDFNKGEYIKFFDYDKIKGEIVVKSREDGDRF
SPLGMRGTKKLKEFFIDEKIPREERDYIPLVAIGKEIVWVVGYRMSEKFKVDKNTSKVLVIEYTKE

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family

Homologues:

Organism=Escherichia coli, GI1786386, Length=218, Percent_Identity=30.7339449541284, Blast_Score=93, Evalue=4e-20,
Organism=Escherichia coli, GI1787606, Length=179, Percent_Identity=27.9329608938547, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TILS_THETN (Q8R7K9)

Other databases:

- EMBL:   AE008691
- RefSeq:   NP_623930.1
- ProteinModelPortal:   Q8R7K9
- SMR:   Q8R7K9
- GeneID:   997364
- GenomeReviews:   AE008691_GR
- KEGG:   tte:TTE2395
- NMPDR:   fig|273068.3.peg.1820
- HOGENOM:   HBG668702
- OMA:   NDISYPL
- ProtClustDB:   CLSK2459011
- BioCyc:   TTEN273068:TTE2395-MONOMER
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_01161
- InterPro:   IPR012094
- InterPro:   IPR012796
- InterPro:   IPR012795
- InterPro:   IPR020825
- InterPro:   IPR014729
- InterPro:   IPR011063
- Gene3D:   G3DSA:3.50.40.10
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11807:SF2
- SMART:   SM00977
- TIGRFAMs:   TIGR02433
- TIGRFAMs:   TIGR02432

Pfam domain/function: PF01171 ATP_bind_3; PF11734 TilS_C; SSF56037 B3_4

EC number: NA

Molecular weight: Translated: 54356; Mature: 54225

Theoretical pI: Translated: 8.67; Mature: 8.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMSFQRFGMIEKVLRTIEKYNMIEKDDKIVMGISGGPDSLCMLDVLFNLKGKFNLKLYV
CCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCHHHHHHHHHCCCCCEEEEEEE
VHVNHMIRGEDAKKDAEFVEKLCKDLDLPFFLFEENIPYLAKKMGLSEEQAGRYVRYKAF
EEEEEEECCCCCCCHHHHHHHHHHHCCCCEEEEECCCCHHHHHCCCCHHHCCCEEEHHHH
EETLKRVGGNKIAVAHNKNDVAETVLLNILRGTGLRGLIGIKPVNGNIIRPLIEIERREI
HHHHHHCCCCEEEEEECCCHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHH
EKYLKDKNLHPRIDHTNYEDLYTRNKIRLKVIPYIEEVFKIDLVENLSRMAAILLEEDDY
HHHHHCCCCCCCCCCCCCHHHHCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCH
LEAKCEEVFNQICEINGEEIKVDVDALKSQHTAIKRRLVRRMYFYVKGETDGLEYGHVED
HHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCHHH
VLNLLDKPTSSKIDLPFEIEALKMYNNLVIRKKKTKEKVKFKEVLKIPGVTTIEGIGKFK
HHHHHCCCCCCCCCCCEEHHHHHHHHHHEEEEHHHHHHHHHHHHHHCCCCCCHHHCCCEE
AYVVDISQVDDFNKGEYIKFFDYDKIKGEIVVKSREDGDRFSPLGMRGTKKLKEFFIDEK
EEEEEHHHCCCCCCCCEEEEEECCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHCCC
IPREERDYIPLVAIGKEIVWVVGYRMSEKFKVDKNTSKVLVIEYTKE
CCCCCCCCCCEEECCCCEEEEECCCCCCCEEECCCCCEEEEEEECCC
>Mature Secondary Structure 
TMSFQRFGMIEKVLRTIEKYNMIEKDDKIVMGISGGPDSLCMLDVLFNLKGKFNLKLYV
CCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCHHHHHHHHHCCCCCEEEEEEE
VHVNHMIRGEDAKKDAEFVEKLCKDLDLPFFLFEENIPYLAKKMGLSEEQAGRYVRYKAF
EEEEEEECCCCCCCHHHHHHHHHHHCCCCEEEEECCCCHHHHHCCCCHHHCCCEEEHHHH
EETLKRVGGNKIAVAHNKNDVAETVLLNILRGTGLRGLIGIKPVNGNIIRPLIEIERREI
HHHHHHCCCCEEEEEECCCHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHH
EKYLKDKNLHPRIDHTNYEDLYTRNKIRLKVIPYIEEVFKIDLVENLSRMAAILLEEDDY
HHHHHCCCCCCCCCCCCCHHHHCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCH
LEAKCEEVFNQICEINGEEIKVDVDALKSQHTAIKRRLVRRMYFYVKGETDGLEYGHVED
HHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCHHH
VLNLLDKPTSSKIDLPFEIEALKMYNNLVIRKKKTKEKVKFKEVLKIPGVTTIEGIGKFK
HHHHHCCCCCCCCCCCEEHHHHHHHHHHEEEEHHHHHHHHHHHHHHCCCCCCHHHCCCEE
AYVVDISQVDDFNKGEYIKFFDYDKIKGEIVVKSREDGDRFSPLGMRGTKKLKEFFIDEK
EEEEEHHHCCCCCCCCEEEEEECCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHCCC
IPREERDYIPLVAIGKEIVWVVGYRMSEKFKVDKNTSKVLVIEYTKE
CCCCCCCCCCEEECCCCEEEEECCCCCCCEEECCCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11997336