Click here to switch to the map view.

The map label for this gene is queD [H]

Identifier: 20808732

GI number: 20808732

Start: 2259053

End: 2259421

Strand: Reverse

Name: queD [H]

Synonym: TTE2368

Alternate gene names: 20808732

Gene position: 2259421-2259053 (Counterclockwise)

Preceding gene: 20808733

Following gene: 20808731

Centisome position: 84.01

GC content: 37.67

Gene sequence:

>369_bases
GTGAGAGTTACAAAAATATTTACCTTTGACAGTGCCCACAACCTTGTTAATTACCATGGGAAATGTGAAGAGTTGCACGG
CCATACTTATAGGTTAGAGGTCACAGTTGAGGGCTATCCTGATGAGGAAGGAATGGTTTTGGACTTTGTAAAGCTAAAAG
AAATTGTCAATGAAAAAATTATAAAAAAGCTTGACCACAAATATTTAAATGAAGTGCTAGGCTTTAACACAACTTGTGAA
AATATACTCCTGTGGATGTGGAAGGAATTAGAAGGAGAACTCAAAGGACCCAATTACAAACTCTACAAGCTTACTCTCTG
GGAGACTCCAACCAGTTTTGCAGAAGTTACAGCAGAGGATTTGAAATGA

Upstream 100 bases:

>100_bases
GAGTAAAAACGGTTTATCCATCGATGAGTTGATACAAGGCCTTATAGACAAGCAGGGCGTATGGCTCTACAAAAAGGATT
GGTGTGGAGAGGTGTAAAAA

Downstream 100 bases:

>100_bases
GGGTTTATGATGATTATTAAAAATCCGATAATAAAAGGGAAATTCATAGAAAGGTTAAATAGATTTGAAGCCTATGTTGA
AATAGATGGCATTAAAACCC

Product: 6-pyruvoyl-tetrahydropterin synthase

Products: 6-pyruvoyl-5,6,7,8-tetrahydropterin; triphosphate

Alternate protein names: CPH4 synthase; Queuosine biosynthesis protein queD [H]

Number of amino acids: Translated: 122; Mature: 122

Protein sequence:

>122_residues
MRVTKIFTFDSAHNLVNYHGKCEELHGHTYRLEVTVEGYPDEEGMVLDFVKLKEIVNEKIIKKLDHKYLNEVLGFNTTCE
NILLWMWKELEGELKGPNYKLYKLTLWETPTSFAEVTAEDLK

Sequences:

>Translated_122_residues
MRVTKIFTFDSAHNLVNYHGKCEELHGHTYRLEVTVEGYPDEEGMVLDFVKLKEIVNEKIIKKLDHKYLNEVLGFNTTCE
NILLWMWKELEGELKGPNYKLYKLTLWETPTSFAEVTAEDLK
>Mature_122_residues
MRVTKIFTFDSAHNLVNYHGKCEELHGHTYRLEVTVEGYPDEEGMVLDFVKLKEIVNEKIIKKLDHKYLNEVLGFNTTCE
NILLWMWKELEGELKGPNYKLYKLTLWETPTSFAEVTAEDLK

Specific function: Catalyzes the conversion of 7,8-dihydroneopterin triphosphate (H2NTP) to 6-carboxy-5,6,7,8-tetrahydropterin (CPH4) and acetaldehyde [H]

COG id: COG0720

COG function: function code H; 6-pyruvoyl-tetrahydropterin synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PTPS family. QueD subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789124, Length=92, Percent_Identity=40.2173913043478, Blast_Score=59, Evalue=8e-11,
Organism=Drosophila melanogaster, GI19550074, Length=98, Percent_Identity=38.7755102040816, Blast_Score=66, Evalue=5e-12,
Organism=Drosophila melanogaster, GI24585378, Length=98, Percent_Identity=38.7755102040816, Blast_Score=66, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007116
- InterPro:   IPR007115 [H]

Pfam domain/function: PF01242 PTPS [H]

EC number: 4.2.3.12

Molecular weight: Translated: 14354; Mature: 14354

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVTKIFTFDSAHNLVNYHGKCEELHGHTYRLEVTVEGYPDEEGMVLDFVKLKEIVNEKI
CCEEEEEEECCCCCHHCCCCCCHHCCCCEEEEEEEECCCCCCCCEEEHHHHHHHHHHHHH
IKKLDHKYLNEVLGFNTTCENILLWMWKELEGELKGPNYKLYKLTLWETPTSFAEVTAED
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEEEEECCCCHHHHHHHHH
LK
CC
>Mature Secondary Structure
MRVTKIFTFDSAHNLVNYHGKCEELHGHTYRLEVTVEGYPDEEGMVLDFVKLKEIVNEKI
CCEEEEEEECCCCCHHCCCCCCHHCCCCEEEEEEEECCCCCCCCEEEHHHHHHHHHHHHH
IKKLDHKYLNEVLGFNTTCENILLWMWKELEGELKGPNYKLYKLTLWETPTSFAEVTAED
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEEEEECCCCHHHHHHHHH
LK
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Mg2+; Zn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 17.7 {6-(L-erythro-1,2-dihydroxypropyl} 5 {6-(L-erythro-1,2-dihydroxypropyl} 0.0091 {6-(L-erythro-1,2-dihydroxypropyl} 0.008 {6-(L-erythro-1,2-dihydroxypropyl} [C]

Substrates: 6-[(1S,2R)-1,2-dihydroxy-3-triphosphooxypropyl]-7,8-dihydropterin

Specific reaction: 6-[(1S,2R)-1,2-dihydroxy-3-triphosphooxypropyl]-7,8-dihydropterin = 6-pyruvoyl-5,6,7,8-tetrahydropterin + triphosphate

General reaction: Intramolecular redox reaction; P-O bond cleavage; Triphosphate elimination [C]

Inhibitor: (NH4)2SO4 [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]