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The map label for this gene is ClpA [H]

Identifier: 20808693

GI number: 20808693

Start: 2208639

End: 2211089

Strand: Reverse

Name: ClpA [H]

Synonym: TTE2327

Alternate gene names: 20808693

Gene position: 2211089-2208639 (Counterclockwise)

Preceding gene: 20808694

Following gene: 20808692

Centisome position: 82.21

GC content: 41.13

Gene sequence:

>2451_bases
ATGGCCATGTTTGGCAGGTTTACAGAAAGGGCTCAAAAGGCTCTTTATCTAGCTCAAGAGGAAGCAAGGTCTTTTTACCA
TAACTATGTTGGAACAGAGCACATCCTATTAGGTTTACTTAAAGAGGACGAAGGGATTGCAGCAAGAGTTTTAAAAAAAT
TGGGAGTGACTTATGAGGCTACAAGAGAAAAAGTCCTTTCTTTAATTGGAATGGGGAACATTCCTGGAGATGTAGTAGGC
TATACGCCGAGAGCTAAAAGGGTTCTGGAACTTAGCTTGAGTGAAGCCAGGAGATTTAACACAAGCTATGTGGGCACAGA
GCACATTTTGCTTGGGCTTTTAAGAGAAGGAGAAGGAGTTGCCGTAAGGATTTTAATGGAGCAGGGAATAGATTTTAACA
GAGTGAGAGAAGAGATTGTAAAAATGCTAAGCGAGGAACCAGCAGCTGGGCCTTCTAAAGTTTCAAAAGCTAAAAACACC
AATACTCCTACTCTCAATCAGTTTGGAAGAGACTTGACTGACCTTGCAAGAGACGGAAAACTTGACCCTGTGATAGGAAG
AGAGAAGGAAATTGAAAGGGTAATACAGATTTTGAGCAGGAGGACAAAAAACAATCCTGTGCTCATAGGAGAGCCGGGAG
TTGGTAAAACTGCCATCGTAGAAGGACTGGCTCAAAAAATTGTCGAGGGTGAAGTTCCTGAGATATTAAAGGATAAAAGA
GTGGTTACCCTAGATTTAGCTTCACTGGTTGCTGGAACTAAATACAGAGGAGAATTTGAAGATAGATTAAAAACTGTAAT
AAATGAGGTAATAAAGGCAGGAAATGTTATACTTTTTGTAGATGAGATGCACACATTAATAGGTGCTGGAGCTGCTGAAG
GTGCAATAGATGCTTCCAACATCTTAAAGCCTGCTCTGGCAAGAGGAGAAATTCAGGTAATTGGTGCTACCACATTGGAT
GAATACAGAAAATATGTGGAAAGAGACCCGGCTTTAGAAAGAAGATTCCAACCCATAATGGTAGAAGAGCCTACTGTAGA
AGAGACGATTGAAATACTAAAAGGGCTAAGAGACAAATATGAAGCTCACCACAGAGTTAAAATTACTGATGAGGCATTGG
AAGCAGCAGCAAAGCTTTCTCACAGGTACATCACAGACAGGTTCCTTCCAGATAAGGCGATTGATTTGATAGATGAAGCT
GCCTCTCGAGTGAGATTAAAAACCGTCACTGCTCCTCCTGAGATAAAAGAATTGGAGGACAAGATTAACGACCTCATAAA
GGAAAAAGAAGAAGCTATAAGGACTCAGGAATATGAAAAAGCTGCTAAAATAAGGGACGAAGAACAGAAATTGAGAGAAC
AGCTGGAGGAATTAAAAGCTAAATGGCAGCAGAATTCTCTCTCTGAAGAAAAGAGCGTTGGTCCAGAAGAAATAGCCCAA
GTCGTATCCATGTGGACTGGAATACCTGTGAAGAAGCTAGCAGAAGAAGAGTCAGAGAGACTCTTACACTTGGAAGAAAT
ACTGCATGAAAGAGTAATCGGACAGGACGAAGCAGTAGAGGCAGTGGCAAAGGCTATAAGAAGGGCAAGAGTAGGGTTAA
AAGATCCAAAAAGGCCGATAGGGTCCTTCATATTCTTAGGACCTACAGGTGTTGGTAAGACGGAGCTCAGTAAGGCTTTA
GCAGAAGCTCTGTTTGGCGATGAAAATGCCATGATAAGATTAGATATGTCAGAATACATGGAAAGGCATACAGTTTCAAA
ACTCATAGGCTCTCCTCCAGGTTATGTGGGCTTTGAAGAAGGAGGACAGCTTACAGAAAAAGTAAGAAGGAGACCTTATT
CAGTGATACTCTTGGACGAGATTGAAAAAGCGCATCCAGATGTTTTCAACATTCTCCTCCAGATACTTGAAGACGGAAGG
TTGACTGACTCTAAGGGAAGGACTGTAGATTTCAAAAACACAGTTATAATCATGACATCAAATGTCGGTGCAGAACTATT
GAAGAAGCAGACTACACTTGGATTTATGCCACAGGAAAAAGAGGACAAGGCTTCTTTTGAGAAGATCAAAGAGACATTGA
TGGCAGAGCTTAGAAAAACCTTCAGACCAGAGTTTTTAAACAGGGTAGACGAAATAATTGTCTTCCATCCGCTTTCTAAA
GAGCACATTGAGAAGATTGCCGACATAATGATAAAGGAATTGAATAAGAGGTTAGAGGAAAACAACATTAAGCTAGAGTT
TACTCCGGAAGCTAAAGAAGAAATCATCAAACAAGGTTACGACCCCAACTACGGCGCAAGACCTTTGAGACGGGTTATAC
AGAGGATTGTAGAAAATCAGCTGTCTGAATTGATGCTTCAGGGAGAAGTCAAACCAGGTGATGAGCTCATCGTCACAGCT
GAAGATGGAAAGATAAAGTTTGTCAAAAAGGATGTGGTTCAAAAGGCTTAG

Upstream 100 bases:

>100_bases
TTTACGGCATGCAACTTAACCCTTATGAGAGGGATGTGAAAAGAGCTGAATATGTGTCCACTCAGTTAAATAAAAAAGAA
TAATTTCGGGAGGTGTTAAT

Downstream 100 bases:

>100_bases
CCGACTGGCTAGGCCTTTTTTATTTTTTTGCACAAGGACAAATAAAAGCACATATTGTTAGTAAAGTGAGCTGGTGTGTT
TTTTAAGATGAGGGGGGATT

Product: ATPase with chaperone activity, ATP-binding subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 816; Mature: 815

Protein sequence:

>816_residues
MAMFGRFTERAQKALYLAQEEARSFYHNYVGTEHILLGLLKEDEGIAARVLKKLGVTYEATREKVLSLIGMGNIPGDVVG
YTPRAKRVLELSLSEARRFNTSYVGTEHILLGLLREGEGVAVRILMEQGIDFNRVREEIVKMLSEEPAAGPSKVSKAKNT
NTPTLNQFGRDLTDLARDGKLDPVIGREKEIERVIQILSRRTKNNPVLIGEPGVGKTAIVEGLAQKIVEGEVPEILKDKR
VVTLDLASLVAGTKYRGEFEDRLKTVINEVIKAGNVILFVDEMHTLIGAGAAEGAIDASNILKPALARGEIQVIGATTLD
EYRKYVERDPALERRFQPIMVEEPTVEETIEILKGLRDKYEAHHRVKITDEALEAAAKLSHRYITDRFLPDKAIDLIDEA
ASRVRLKTVTAPPEIKELEDKINDLIKEKEEAIRTQEYEKAAKIRDEEQKLREQLEELKAKWQQNSLSEEKSVGPEEIAQ
VVSMWTGIPVKKLAEEESERLLHLEEILHERVIGQDEAVEAVAKAIRRARVGLKDPKRPIGSFIFLGPTGVGKTELSKAL
AEALFGDENAMIRLDMSEYMERHTVSKLIGSPPGYVGFEEGGQLTEKVRRRPYSVILLDEIEKAHPDVFNILLQILEDGR
LTDSKGRTVDFKNTVIIMTSNVGAELLKKQTTLGFMPQEKEDKASFEKIKETLMAELRKTFRPEFLNRVDEIIVFHPLSK
EHIEKIADIMIKELNKRLEENNIKLEFTPEAKEEIIKQGYDPNYGARPLRRVIQRIVENQLSELMLQGEVKPGDELIVTA
EDGKIKFVKKDVVQKA

Sequences:

>Translated_816_residues
MAMFGRFTERAQKALYLAQEEARSFYHNYVGTEHILLGLLKEDEGIAARVLKKLGVTYEATREKVLSLIGMGNIPGDVVG
YTPRAKRVLELSLSEARRFNTSYVGTEHILLGLLREGEGVAVRILMEQGIDFNRVREEIVKMLSEEPAAGPSKVSKAKNT
NTPTLNQFGRDLTDLARDGKLDPVIGREKEIERVIQILSRRTKNNPVLIGEPGVGKTAIVEGLAQKIVEGEVPEILKDKR
VVTLDLASLVAGTKYRGEFEDRLKTVINEVIKAGNVILFVDEMHTLIGAGAAEGAIDASNILKPALARGEIQVIGATTLD
EYRKYVERDPALERRFQPIMVEEPTVEETIEILKGLRDKYEAHHRVKITDEALEAAAKLSHRYITDRFLPDKAIDLIDEA
ASRVRLKTVTAPPEIKELEDKINDLIKEKEEAIRTQEYEKAAKIRDEEQKLREQLEELKAKWQQNSLSEEKSVGPEEIAQ
VVSMWTGIPVKKLAEEESERLLHLEEILHERVIGQDEAVEAVAKAIRRARVGLKDPKRPIGSFIFLGPTGVGKTELSKAL
AEALFGDENAMIRLDMSEYMERHTVSKLIGSPPGYVGFEEGGQLTEKVRRRPYSVILLDEIEKAHPDVFNILLQILEDGR
LTDSKGRTVDFKNTVIIMTSNVGAELLKKQTTLGFMPQEKEDKASFEKIKETLMAELRKTFRPEFLNRVDEIIVFHPLSK
EHIEKIADIMIKELNKRLEENNIKLEFTPEAKEEIIKQGYDPNYGARPLRRVIQRIVENQLSELMLQGEVKPGDELIVTA
EDGKIKFVKKDVVQKA
>Mature_815_residues
AMFGRFTERAQKALYLAQEEARSFYHNYVGTEHILLGLLKEDEGIAARVLKKLGVTYEATREKVLSLIGMGNIPGDVVGY
TPRAKRVLELSLSEARRFNTSYVGTEHILLGLLREGEGVAVRILMEQGIDFNRVREEIVKMLSEEPAAGPSKVSKAKNTN
TPTLNQFGRDLTDLARDGKLDPVIGREKEIERVIQILSRRTKNNPVLIGEPGVGKTAIVEGLAQKIVEGEVPEILKDKRV
VTLDLASLVAGTKYRGEFEDRLKTVINEVIKAGNVILFVDEMHTLIGAGAAEGAIDASNILKPALARGEIQVIGATTLDE
YRKYVERDPALERRFQPIMVEEPTVEETIEILKGLRDKYEAHHRVKITDEALEAAAKLSHRYITDRFLPDKAIDLIDEAA
SRVRLKTVTAPPEIKELEDKINDLIKEKEEAIRTQEYEKAAKIRDEEQKLREQLEELKAKWQQNSLSEEKSVGPEEIAQV
VSMWTGIPVKKLAEEESERLLHLEEILHERVIGQDEAVEAVAKAIRRARVGLKDPKRPIGSFIFLGPTGVGKTELSKALA
EALFGDENAMIRLDMSEYMERHTVSKLIGSPPGYVGFEEGGQLTEKVRRRPYSVILLDEIEKAHPDVFNILLQILEDGRL
TDSKGRTVDFKNTVIIMTSNVGAELLKKQTTLGFMPQEKEDKASFEKIKETLMAELRKTFRPEFLNRVDEIIVFHPLSKE
HIEKIADIMIKELNKRLEENNIKLEFTPEAKEEIIKQGYDPNYGARPLRRVIQRIVENQLSELMLQGEVKPGDELIVTAE
DGKIKFVKKDVVQKA

Specific function: Part of a stress-induced multi-chaperone system, it is involved in the recovery of the cell from heat-induced damage, in cooperation with DnaK, DnaJ and GrpE. Acts before DnaK, in the processing of protein aggregates. Protein binding stimulates the ATPase

COG id: COG0542

COG function: function code O; ATPases with chaperone activity, ATP-binding subunit

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 UVR domain [H]

Homologues:

Organism=Homo sapiens, GI13540606, Length=332, Percent_Identity=40.9638554216867, Blast_Score=224, Evalue=3e-58,
Organism=Escherichia coli, GI1788943, Length=853, Percent_Identity=48.5345838218054, Blast_Score=766, Evalue=0.0,
Organism=Escherichia coli, GI1787109, Length=803, Percent_Identity=39.227895392279, Blast_Score=540, Evalue=1e-154,
Organism=Saccharomyces cerevisiae, GI6320464, Length=706, Percent_Identity=50.1416430594901, Blast_Score=675, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6323002, Length=397, Percent_Identity=42.8211586901763, Blast_Score=316, Evalue=1e-86,

Paralogues:

None

Copy number: 560 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013093
- InterPro:   IPR003959
- InterPro:   IPR018368
- InterPro:   IPR001270
- InterPro:   IPR019489
- InterPro:   IPR004176
- InterPro:   IPR023150
- InterPro:   IPR001943 [H]

Pfam domain/function: PF00004 AAA; PF07724 AAA_2; PF02861 Clp_N; PF10431 ClpB_D2-small; PF02151 UVR [H]

EC number: NA

Molecular weight: Translated: 92040; Mature: 91909

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: PS50151 UVR ; PS00870 CLPAB_1 ; PS00871 CLPAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAMFGRFTERAQKALYLAQEEARSFYHNYVGTEHILLGLLKEDEGIAARVLKKLGVTYEA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHCCCHHH
TREKVLSLIGMGNIPGDVVGYTPRAKRVLELSLSEARRFNTSYVGTEHILLGLLREGEGV
HHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCC
AVRILMEQGIDFNRVREEIVKMLSEEPAAGPSKVSKAKNTNTPTLNQFGRDLTDLARDGK
EEEEHHHCCCCHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCHHHHCCHHHHHHHCCC
LDPVIGREKEIERVIQILSRRTKNNPVLIGEPGVGKTAIVEGLAQKIVEGEVPEILKDKR
CCCCCCCHHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCHHHHHCCC
VVTLDLASLVAGTKYRGEFEDRLKTVINEVIKAGNVILFVDEMHTLIGAGAAEGAIDASN
EEEEEHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCEEEEHHHHHHHHHCCCCCCCCCHHH
ILKPALARGEIQVIGATTLDEYRKYVERDPALERRFQPIMVEEPTVEETIEILKGLRDKY
HHHHHHCCCCEEEEECCCHHHHHHHHHCCHHHHHCCCCCEECCCCHHHHHHHHHHHHHHH
EAHHRVKITDEALEAAAKLSHRYITDRFLPDKAIDLIDEAASRVRLKTVTAPPEIKELED
HCCCCEEECHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHEEEEEECCCCHHHHHHH
KINDLIKEKEEAIRTQEYEKAAKIRDEEQKLREQLEELKAKWQQNSLSEEKSVGPEEIAQ
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHCCCHHHHHH
VVSMWTGIPVKKLAEEESERLLHLEEILHERVIGQDEAVEAVAKAIRRARVGLKDPKRPI
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCC
GSFIFLGPTGVGKTELSKALAEALFGDENAMIRLDMSEYMERHTVSKLIGSPPGYVGFEE
CCEEEECCCCCCHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHHCCCCCCCCCCC
GGQLTEKVRRRPYSVILLDEIEKAHPDVFNILLQILEDGRLTDSKGRTVDFKNTVIIMTS
CCHHHHHHHHCCCEEEEEHHHHHHCCHHHHHHHHHHHCCCCCCCCCCEEEECCEEEEEEC
NVGAELLKKQTTLGFMPQEKEDKASFEKIKETLMAELRKTFRPEFLNRVDEIIVFHPLSK
CCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHEEEECCCCH
EHIEKIADIMIKELNKRLEENNIKLEFTPEAKEEIIKQGYDPNYGARPLRRVIQRIVENQ
HHHHHHHHHHHHHHHHHHHHCCCEEEECCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHH
LSELMLQGEVKPGDELIVTAEDGKIKFVKKDVVQKA
HHHHHHHCCCCCCCCEEEEECCCCEEHHHHHHHCCC
>Mature Secondary Structure 
AMFGRFTERAQKALYLAQEEARSFYHNYVGTEHILLGLLKEDEGIAARVLKKLGVTYEA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHCCCHHH
TREKVLSLIGMGNIPGDVVGYTPRAKRVLELSLSEARRFNTSYVGTEHILLGLLREGEGV
HHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCC
AVRILMEQGIDFNRVREEIVKMLSEEPAAGPSKVSKAKNTNTPTLNQFGRDLTDLARDGK
EEEEHHHCCCCHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCHHHHCCHHHHHHHCCC
LDPVIGREKEIERVIQILSRRTKNNPVLIGEPGVGKTAIVEGLAQKIVEGEVPEILKDKR
CCCCCCCHHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCHHHHHCCC
VVTLDLASLVAGTKYRGEFEDRLKTVINEVIKAGNVILFVDEMHTLIGAGAAEGAIDASN
EEEEEHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCEEEEHHHHHHHHHCCCCCCCCCHHH
ILKPALARGEIQVIGATTLDEYRKYVERDPALERRFQPIMVEEPTVEETIEILKGLRDKY
HHHHHHCCCCEEEEECCCHHHHHHHHHCCHHHHHCCCCCEECCCCHHHHHHHHHHHHHHH
EAHHRVKITDEALEAAAKLSHRYITDRFLPDKAIDLIDEAASRVRLKTVTAPPEIKELED
HCCCCEEECHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHEEEEEECCCCHHHHHHH
KINDLIKEKEEAIRTQEYEKAAKIRDEEQKLREQLEELKAKWQQNSLSEEKSVGPEEIAQ
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHCCCHHHHHH
VVSMWTGIPVKKLAEEESERLLHLEEILHERVIGQDEAVEAVAKAIRRARVGLKDPKRPI
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCC
GSFIFLGPTGVGKTELSKALAEALFGDENAMIRLDMSEYMERHTVSKLIGSPPGYVGFEE
CCEEEECCCCCCHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHHCCCCCCCCCCC
GGQLTEKVRRRPYSVILLDEIEKAHPDVFNILLQILEDGRLTDSKGRTVDFKNTVIIMTS
CCHHHHHHHHCCCEEEEEHHHHHHCCHHHHHHHHHHHCCCCCCCCCCEEEECCEEEEEEC
NVGAELLKKQTTLGFMPQEKEDKASFEKIKETLMAELRKTFRPEFLNRVDEIIVFHPLSK
CCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHEEEECCCCH
EHIEKIADIMIKELNKRLEENNIKLEFTPEAKEEIIKQGYDPNYGARPLRRVIQRIVENQ
HHHHHHHHHHHHHHHHHHHHCCCEEEECCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHH
LSELMLQGEVKPGDELIVTAEDGKIKFVKKDVVQKA
HHHHHHHCCCCCCCCEEEEECCCCEEHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Serine endopeptidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12235376 [H]