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The map label for this gene is ispF

Identifier: 20808686

GI number: 20808686

Start: 2201537

End: 2202019

Strand: Reverse

Name: ispF

Synonym: TTE2320

Alternate gene names: 20808686

Gene position: 2202019-2201537 (Counterclockwise)

Preceding gene: 20808687

Following gene: 20808685

Centisome position: 81.88

GC content: 40.58

Gene sequence:

>483_bases
ATGGGTAAAATGAGAGTAGGGATAGGATATGATGTCCACAGGTTTGAAGAAGGAAAAAAATTGATGCTCGGAGGGATAGA
AATCCCCTTTGAAAAAGGGTTAAAGGGGCATTCTGATGGAGATGTTCTTATTCACGCTTTAATTGATGGGATTTTGGGAG
CTGCGGGTTTGGGAGATATTGGAGAACATTTTCCTGATACAGATGTGGCCTATAAGGAAGTAGACAGTAAAATACTTTTA
AAAAGGGTGTTGGAATTAGTGAGTGAGCACTATGTTGTGAACAACGTGGATTGCATAGTTGTAACCGAAAGGCCCAAGCT
TTCTCCTTATAAAGACAGAATAAGGACAAGCCTTGCAGAATTGCTTGGTGTTAGCAAAGAAAGAGTGAATGTCAAAGCAA
AGACGGAGGAAGGATTGGGATTTACAGGAAGAGAGGAAGGCATAAAGGCTTATGCTGTAGTATTACTGGAGGAAAGGTAT
TGA

Upstream 100 bases:

>100_bases
AATAAAAAAACCGTAGAGAAAAAATTTAAGTCCTCTCGCCTGTCAAGAGGGGCAGAATTTACTTTAGAAGTGGATGAGGA
AGGAAACTTTAAAATGAGGG

Downstream 100 bases:

>100_bases
CATATAACCTGCCGTAAAATACAATCTACTTAAAAAATCTCGTACATAGGAGGGTAAAGAGAGTGAGACTGTCACAGTTA
CTAATGCCAACTTTAAGAGA

Product: 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Products: NA

Alternate protein names: MECDP-synthase; MECPS

Number of amino acids: Translated: 160; Mature: 159

Protein sequence:

>160_residues
MGKMRVGIGYDVHRFEEGKKLMLGGIEIPFEKGLKGHSDGDVLIHALIDGILGAAGLGDIGEHFPDTDVAYKEVDSKILL
KRVLELVSEHYVVNNVDCIVVTERPKLSPYKDRIRTSLAELLGVSKERVNVKAKTEEGLGFTGREEGIKAYAVVLLEERY

Sequences:

>Translated_160_residues
MGKMRVGIGYDVHRFEEGKKLMLGGIEIPFEKGLKGHSDGDVLIHALIDGILGAAGLGDIGEHFPDTDVAYKEVDSKILL
KRVLELVSEHYVVNNVDCIVVTERPKLSPYKDRIRTSLAELLGVSKERVNVKAKTEEGLGFTGREEGIKAYAVVLLEERY
>Mature_159_residues
GKMRVGIGYDVHRFEEGKKLMLGGIEIPFEKGLKGHSDGDVLIHALIDGILGAAGLGDIGEHFPDTDVAYKEVDSKILLK
RVLELVSEHYVVNNVDCIVVTERPKLSPYKDRIRTSLAELLGVSKERVNVKAKTEEGLGFTGREEGIKAYAVVLLEERY

Specific function: Converts 4-diphosphocytidyl-2-C-methyl-D-erythritol 2- phosphate into 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (MECDP) and CMP

COG id: COG0245

COG function: function code I; 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispF family

Homologues:

Organism=Escherichia coli, GI1789103, Length=154, Percent_Identity=50.6493506493506, Blast_Score=148, Evalue=2e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPF_THETN (Q8R7S8)

Other databases:

- EMBL:   AE008691
- RefSeq:   NP_623857.1
- ProteinModelPortal:   Q8R7S8
- SMR:   Q8R7S8
- GeneID:   997849
- GenomeReviews:   AE008691_GR
- KEGG:   tte:TTE2320
- NMPDR:   fig|273068.3.peg.2498
- HOGENOM:   HBG630070
- OMA:   HKFGGEG
- ProtClustDB:   PRK00084
- BioCyc:   TTEN273068:TTE2320-MONOMER
- BRENDA:   4.6.1.12
- HAMAP:   MF_00107
- InterPro:   IPR003526
- InterPro:   IPR020555
- Gene3D:   G3DSA:3.30.1330.50
- TIGRFAMs:   TIGR00151

Pfam domain/function: PF02542 YgbB; SSF69765 YgbB_synth

EC number: =4.6.1.12

Molecular weight: Translated: 17693; Mature: 17562

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: PS01350 ISPF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKMRVGIGYDVHRFEEGKKLMLGGIEIPFEKGLKGHSDGDVLIHALIDGILGAAGLGDI
CCCEEECCCCCHHHHHCCCEEEECCEECCHHHCCCCCCCCHHHHHHHHHHHHCCCCCCHH
GEHFPDTDVAYKEVDSKILLKRVLELVSEHYVVNNVDCIVVTERPKLSPYKDRIRTSLAE
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEECCCCEEEEECCCCCCHHHHHHHHHHHH
LLGVSKERVNVKAKTEEGLGFTGREEGIKAYAVVLLEERY
HHCCCHHHEEEEEECCCCCCCCCCHHCCEEEEEEEEECCC
>Mature Secondary Structure 
GKMRVGIGYDVHRFEEGKKLMLGGIEIPFEKGLKGHSDGDVLIHALIDGILGAAGLGDI
CCEEECCCCCHHHHHCCCEEEECCEECCHHHCCCCCCCCHHHHHHHHHHHHCCCCCCHH
GEHFPDTDVAYKEVDSKILLKRVLELVSEHYVVNNVDCIVVTERPKLSPYKDRIRTSLAE
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEECCCCEEEEECCCCCCHHHHHHHHHHHH
LLGVSKERVNVKAKTEEGLGFTGREEGIKAYAVVLLEERY
HHCCCHHHEEEEEECCCCCCCCCCHHCCEEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11997336