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The map label for this gene is ProS

Identifier: 20808685

GI number: 20808685

Start: 2199753

End: 2201474

Strand: Reverse

Name: ProS

Synonym: TTE2319

Alternate gene names: 20808685

Gene position: 2201474-2199753 (Counterclockwise)

Preceding gene: 20808686

Following gene: 20808684

Centisome position: 81.86

GC content: 40.3

Gene sequence:

>1722_bases
GTGAGACTGTCACAGTTACTAATGCCAACTTTAAGAGAAGTTCCTGCTGATGCTGAAATTCCAAGCCATGTCCTTATGTT
AAAAGCAGGATTGATGAGGAAATTAGCAGCTGGAATATATGTGTATCTGCCTTTAGGAAAAAGAGTACTAAAAAAAGTGG
AAGAGATAGTTAGAGAAGAAATGGACAGAGAAGGAAGTCAGGAAGTCTTGATGTCTGCTTTGATTCCTGCAGAGCTTTTT
AAGGAAACGGGAAGATGGGATGTGTTTGGACCCGAAATGTTTAAGCTTAAAGACAGAAATGAGAGAGATTTTTGTTTGGG
ACCAACTCACGAAGAAGTCTTTACAGACCTTGTAAGAAATGAAGTGAAATCATATAGACAGCTTCCCCTCATACTTTACC
AGATTCAAACTAAATTTAGAGACGAGAGGCGTCCGCGCTTTGGTGTGATGAGGAGCAGAGAGTTTATAATGAAGGACGCT
TACAGCTTTGATGCTGATTGGGAAGGACTAGACGAATCTTTTAATAAAATGTACAGGGCGTACTGCAGAATATTTGACAG
GTGTGGTCTTAAATATCTGGTGGTGGAAGCCGACCCTGGAGCCATGGGAGGAAGAGATTCAAAAGAATTCATGGTGATAT
CCAGTGTTGGAGAAGCGGTGATAGCTTACTGCGACAGCTGTGGATACGCTGCCAATGAGGAGAAGGCCGAGTGTTTAATA
GAAAGTCGGGATGAGGAGATGCTTGAGATTGAAAAAGTTTATACTCCCAATGTCAAGACTATAGAAGAACTGGTAGACTT
CTTAAAAATTTCTCCTAGCAAATTTGTAAAAACTCTCATATACAAAGCAAAAGGCAAAGTTGTAGCAGCTTTGGTTAGAG
GCGATAGAGATATAAACGAGACAAAGCTTTTGAATGTTTTGAGCATAAGGGAAGAGGAACTGGAATTGGCTGATGGAGCT
TTAGTGGAAGAAGTCACAGGGGCGAAGGTGGGCTTTGCTGGTCCCTTGGGATTGAAAGGAGAAGTGACTTTAGTTGTAGA
CAGCGAAATACCACAGTTGAGGAACTTTATCGTGGGAGCCAACGAGACAGATTACCACATAAAAAATGTAAATTATGGCA
GAGATTTTAAAGGCGATATCGTAGCAGATATTAAAAGCGTCGTAGAAGGAGATAGGTGCCCAAGGTGTGGTGCTCCTCTT
AAAATTGCTAGAGGAATTGAGGTTGGTCATATTTTTAAGCTAGGCACAAAGTACAGCGAGGCGCTAGGAGCTACTTACAC
AGATGAGGAAGGGAATGAAAAGCCAATTGTAATGGGATGCTACGGCATTGGAATTAACAGAACTGTAGCTGCAATTATAG
AACAGCACCACGATGAAAAAGGCATAATATGGCCTATGAGTGTAGCGCCTTATCATGTCATTGTAGTACCTGTGAATGTA
TCAGATGAAGAGCAAAAACAGATTGCAGAAAAGATATACAATAAGTTGCTTGAAGAGAAATTAGAGGTTTTGATTGATGA
CAGGGATGTAAGAGCTGGCGTTAAATTTAATGACGCAGATTTGATAGGAATACCTGTAAGAGTAACTATAGGCAAAAAGG
TAAAAGAAGGAATTGTTGAAATAAAATTAAGAGAAAGAGAAGAAGTGGAAGAAGTAAAGGTGGAAGAGGTGGTAAAAAGA
GTAAAACAAATTGTAGAGGAGAAGTTGAGAGAGCTCTCATGA

Upstream 100 bases:

>100_bases
AGGCTTATGCTGTAGTATTACTGGAGGAAAGGTATTGACATATAACCTGCCGTAAAATACAATCTACTTAAAAAATCTCG
TACATAGGAGGGTAAAGAGA

Downstream 100 bases:

>100_bases
GCTCTTTTTTGCATAAAATTCTTTTACAGTGAAAAAATATTTGTTAAAAGATTGATAAAAATGGGGTGAAGGTTGTGTCA
TTGTACATCGTAGGGATAAG

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS

Number of amino acids: Translated: 573; Mature: 573

Protein sequence:

>573_residues
MRLSQLLMPTLREVPADAEIPSHVLMLKAGLMRKLAAGIYVYLPLGKRVLKKVEEIVREEMDREGSQEVLMSALIPAELF
KETGRWDVFGPEMFKLKDRNERDFCLGPTHEEVFTDLVRNEVKSYRQLPLILYQIQTKFRDERRPRFGVMRSREFIMKDA
YSFDADWEGLDESFNKMYRAYCRIFDRCGLKYLVVEADPGAMGGRDSKEFMVISSVGEAVIAYCDSCGYAANEEKAECLI
ESRDEEMLEIEKVYTPNVKTIEELVDFLKISPSKFVKTLIYKAKGKVVAALVRGDRDINETKLLNVLSIREEELELADGA
LVEEVTGAKVGFAGPLGLKGEVTLVVDSEIPQLRNFIVGANETDYHIKNVNYGRDFKGDIVADIKSVVEGDRCPRCGAPL
KIARGIEVGHIFKLGTKYSEALGATYTDEEGNEKPIVMGCYGIGINRTVAAIIEQHHDEKGIIWPMSVAPYHVIVVPVNV
SDEEQKQIAEKIYNKLLEEKLEVLIDDRDVRAGVKFNDADLIGIPVRVTIGKKVKEGIVEIKLREREEVEEVKVEEVVKR
VKQIVEEKLRELS

Sequences:

>Translated_573_residues
MRLSQLLMPTLREVPADAEIPSHVLMLKAGLMRKLAAGIYVYLPLGKRVLKKVEEIVREEMDREGSQEVLMSALIPAELF
KETGRWDVFGPEMFKLKDRNERDFCLGPTHEEVFTDLVRNEVKSYRQLPLILYQIQTKFRDERRPRFGVMRSREFIMKDA
YSFDADWEGLDESFNKMYRAYCRIFDRCGLKYLVVEADPGAMGGRDSKEFMVISSVGEAVIAYCDSCGYAANEEKAECLI
ESRDEEMLEIEKVYTPNVKTIEELVDFLKISPSKFVKTLIYKAKGKVVAALVRGDRDINETKLLNVLSIREEELELADGA
LVEEVTGAKVGFAGPLGLKGEVTLVVDSEIPQLRNFIVGANETDYHIKNVNYGRDFKGDIVADIKSVVEGDRCPRCGAPL
KIARGIEVGHIFKLGTKYSEALGATYTDEEGNEKPIVMGCYGIGINRTVAAIIEQHHDEKGIIWPMSVAPYHVIVVPVNV
SDEEQKQIAEKIYNKLLEEKLEVLIDDRDVRAGVKFNDADLIGIPVRVTIGKKVKEGIVEIKLREREEVEEVKVEEVVKR
VKQIVEEKLRELS
>Mature_573_residues
MRLSQLLMPTLREVPADAEIPSHVLMLKAGLMRKLAAGIYVYLPLGKRVLKKVEEIVREEMDREGSQEVLMSALIPAELF
KETGRWDVFGPEMFKLKDRNERDFCLGPTHEEVFTDLVRNEVKSYRQLPLILYQIQTKFRDERRPRFGVMRSREFIMKDA
YSFDADWEGLDESFNKMYRAYCRIFDRCGLKYLVVEADPGAMGGRDSKEFMVISSVGEAVIAYCDSCGYAANEEKAECLI
ESRDEEMLEIEKVYTPNVKTIEELVDFLKISPSKFVKTLIYKAKGKVVAALVRGDRDINETKLLNVLSIREEELELADGA
LVEEVTGAKVGFAGPLGLKGEVTLVVDSEIPQLRNFIVGANETDYHIKNVNYGRDFKGDIVADIKSVVEGDRCPRCGAPL
KIARGIEVGHIFKLGTKYSEALGATYTDEEGNEKPIVMGCYGIGINRTVAAIIEQHHDEKGIIWPMSVAPYHVIVVPVNV
SDEEQKQIAEKIYNKLLEEKLEVLIDDRDVRAGVKFNDADLIGIPVRVTIGKKVKEGIVEIKLREREEVEEVKVEEVVKR
VKQIVEEKLRELS

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids su

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily

Homologues:

Organism=Homo sapiens, GI34303926, Length=252, Percent_Identity=40.0793650793651, Blast_Score=208, Evalue=9e-54,
Organism=Escherichia coli, GI1786392, Length=573, Percent_Identity=48.8656195462478, Blast_Score=526, Evalue=1e-150,
Organism=Caenorhabditis elegans, GI115532348, Length=303, Percent_Identity=33.6633663366337, Blast_Score=165, Evalue=6e-41,
Organism=Caenorhabditis elegans, GI71991342, Length=172, Percent_Identity=29.0697674418605, Blast_Score=73, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI193203271, Length=85, Percent_Identity=34.1176470588235, Blast_Score=70, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6320931, Length=540, Percent_Identity=32.037037037037, Blast_Score=236, Evalue=6e-63,
Organism=Drosophila melanogaster, GI24656200, Length=214, Percent_Identity=39.7196261682243, Blast_Score=171, Evalue=1e-42,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): SYP_THETN (Q8R7S9)

Other databases:

- EMBL:   AE008691
- RefSeq:   NP_623856.1
- HSSP:   Q58635
- ProteinModelPortal:   Q8R7S9
- SMR:   Q8R7S9
- GeneID:   997686
- GenomeReviews:   AE008691_GR
- KEGG:   tte:TTE2319
- NMPDR:   fig|273068.3.peg.2497
- HOGENOM:   HBG403504
- OMA:   DFVLGPT
- ProtClustDB:   PRK09194
- BioCyc:   TTEN273068:TTE2319-MONOMER
- BRENDA:   6.1.1.15
- GO:   GO:0005737
- HAMAP:   MF_01569
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500
- InterPro:   IPR007214
- Gene3D:   G3DSA:3.40.50.800
- Gene3D:   G3DSA:3.90.960.10
- PRINTS:   PR01046
- TIGRFAMs:   TIGR00409

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF04073 YbaK; SSF52954 Anticodon_bd; SSF55826 YbaK/aa-tRNA-synth-assoc-reg

EC number: =6.1.1.15

Molecular weight: Translated: 64921; Mature: 64921

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLSQLLMPTLREVPADAEIPSHVLMLKAGLMRKLAAGIYVYLPLGKRVLKKVEEIVREE
CCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHH
MDREGSQEVLMSALIPAELFKETGRWDVFGPEMFKLKDRNERDFCLGPTHEEVFTDLVRN
HCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCCCCCCCCCCHHHHHHHHHHH
EVKSYRQLPLILYQIQTKFRDERRPRFGVMRSREFIMKDAYSFDADWEGLDESFNKMYRA
HHHHHHHCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
YCRIFDRCGLKYLVVEADPGAMGGRDSKEFMVISSVGEAVIAYCDSCGYAANEEKAECLI
HHHHHHHCCCEEEEEECCCCCCCCCCCCCEEEEHHHHHHHHHHHHCCCCCCCCHHHHHHH
ESRDEEMLEIEKVYTPNVKTIEELVDFLKISPSKFVKTLIYKAKGKVVAALVRGDRDINE
HCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHCCCEEEEEECCCCCCCH
TKLLNVLSIREEELELADGALVEEVTGAKVGFAGPLGLKGEVTLVVDSEIPQLRNFIVGA
HHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHEECC
NETDYHIKNVNYGRDFKGDIVADIKSVVEGDRCPRCGAPLKIARGIEVGHIFKLGTKYSE
CCCCEEEECCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHCCCCCHHHEECCCHHHH
ALGATYTDEEGNEKPIVMGCYGIGINRTVAAIIEQHHDEKGIIWPMSVAPYHVIVVPVNV
HHCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHCCCCCCEEEECCCCEEEEEEEECC
SDEEQKQIAEKIYNKLLEEKLEVLIDDRDVRAGVKFNDADLIGIPVRVTIGKKVKEGIVE
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEECCCCEEEEEEEEEECHHHHCCHHE
IKLREREEVEEVKVEEVVKRVKQIVEEKLRELS
EEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRLSQLLMPTLREVPADAEIPSHVLMLKAGLMRKLAAGIYVYLPLGKRVLKKVEEIVREE
CCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHH
MDREGSQEVLMSALIPAELFKETGRWDVFGPEMFKLKDRNERDFCLGPTHEEVFTDLVRN
HCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCCCCCCCCCCHHHHHHHHHHH
EVKSYRQLPLILYQIQTKFRDERRPRFGVMRSREFIMKDAYSFDADWEGLDESFNKMYRA
HHHHHHHCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
YCRIFDRCGLKYLVVEADPGAMGGRDSKEFMVISSVGEAVIAYCDSCGYAANEEKAECLI
HHHHHHHCCCEEEEEECCCCCCCCCCCCCEEEEHHHHHHHHHHHHCCCCCCCCHHHHHHH
ESRDEEMLEIEKVYTPNVKTIEELVDFLKISPSKFVKTLIYKAKGKVVAALVRGDRDINE
HCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHCCCEEEEEECCCCCCCH
TKLLNVLSIREEELELADGALVEEVTGAKVGFAGPLGLKGEVTLVVDSEIPQLRNFIVGA
HHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHEECC
NETDYHIKNVNYGRDFKGDIVADIKSVVEGDRCPRCGAPLKIARGIEVGHIFKLGTKYSE
CCCCEEEECCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHCCCCCHHHEECCCHHHH
ALGATYTDEEGNEKPIVMGCYGIGINRTVAAIIEQHHDEKGIIWPMSVAPYHVIVVPVNV
HHCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHCCCCCCEEEECCCCEEEEEEEECC
SDEEQKQIAEKIYNKLLEEKLEVLIDDRDVRAGVKFNDADLIGIPVRVTIGKKVKEGIVE
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEECCCCEEEEEEEEEECHHHHCCHHE
IKLREREEVEEVKVEEVVKRVKQIVEEKLRELS
EEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11997336