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The map label for this gene is cysS

Identifier: 20808681

GI number: 20808681

Start: 2195599

End: 2197002

Strand: Reverse

Name: cysS

Synonym: TTE2315

Alternate gene names: 20808681

Gene position: 2197002-2195599 (Counterclockwise)

Preceding gene: 20808682

Following gene: 20808680

Centisome position: 81.69

GC content: 39.1

Gene sequence:

>1404_bases
ATGAGGCTGTATAACACAATGACTAGAACTAAAGAGGAATTTAAGCCTTTGAAGGATAAAGTGGTAAACATGTATGTCTG
TGGGCCTACAGTTTACAATTACATCCACATAGGGAATGCTAGAGCTTTTATCGTCTTCGATACTGTGAGAAGGTATTTGG
AGTACAAGGGTTACACTGTAAACTACGTTCAAAATTTTACAGATATTGACGATAAGATAATAAATAGAGCGAAGGAGGAG
AATACTACAGAAAAGGAAATAGCAGAAAGGTTCATAGAGGAGTATTTTAAAGATGCAGATGCTCTTGGGATAAAGAGAGC
TACAGTTCACCCCAGGGCCACAGAGCATATAGATGACATAATTGAATTCATAAAAATACTCATTGACAAAGGGTATGCAT
ATGTGGTAGACGGCAATGTGTATTTTGAAACGGCTAAATTTAAGGATTATGGGAAACTTTCTCATAAAAATATAGATGAA
CTGATGGCGGGTGCAAGGGTGGAGATAGACGAGAAAAAGAAAAACCCTCTAGACTTTGCACTGTGGAAGGCTCAAAAGCC
CGGAGAACCTGCTTGGGACAGTCCGTGGGGGAAAGGAAGGCCTGGCTGGCATATTGAGTGCTCTGTCATGTCTACAAAGT
ATTTGGGGAAGACCTTGGACATTCACGCAGGAGGGCCAGATTTAATTTTCCCTCACCATGAAAATGAGATCGCTCAAAGC
GAAGCAGCCTATGACCAGCCGTTTTCTAAATATTGGATGCATATAGGGTATTTAAATGTGAACAATGAGAAGATGTCAAA
GTCAAAGGGTAATTTCTTTACAGTGAGGGAGCTTACGGAGAAATATGACCCTGAAGTTTTAAGGCTTTTCATGCTTATGG
CCCATTACAGGAGTCCTATAAACTTTAGCCTTGATTTGCTAGACCAGGCGAAATCCGCCTATGAAAGACTTAGTAACGCT
GTGGTCAATTTGAAGCACTTGGCAAATATTACCAAAGACAGGGAGTTAAGCGATGAAGAAAAAGATTTGATTGTCAAGTT
TGATGAGTATAAAAAGCAGTTTGAAGAGGCTATGGACGATGATTTTAACACGGCAGATGCTATTTCTGTGCTTTTTGAAA
TGGCTAAAACTGCCAATGCAAATTTAAGTGGAAATTCTTCTAAGAAATTGGTAGAATATATTCTGGAGATGTTCTTAAAG
CTTTCTGATGTATTAGGGCTGTCTTACAAAAACGCTGAGACAGAGATTGAAGATGAAGAAATACTCAGGTTGATAGAGGA
AAGGCAAAAGGCCAGAAAAGAGAAAAACTGGAAGTTAGCTGATGAGATAAGGGATAAATTGAAAGAAAGAGGTATAATTT
TAGAGGATACGCCAGAGGGAGTAAGATGGAAAAGGATAAGATGA

Upstream 100 bases:

>100_bases
GAACATGGCAAGCTCCCTGACCCTGTAGAGGAAGAGCTAAGAAGGTTAAGAGAGAGGATAGAGAGGCTTGAAAAAATTTT
AATGGAAAGGGATGAGGAAG

Downstream 100 bases:

>100_bases
TTCTTGTAAAGGAAAAGGGGGTTTTAGACTTATCCCCCCTTGTTTTGGCTTTCATTGGAGATGCGGTTTACAGCCTTTAT
GTCAGAACTAAGATTGTGGA

Product: cysteinyl-tRNA synthetase

Products: NA

Alternate protein names: Cysteine--tRNA ligase; CysRS

Number of amino acids: Translated: 467; Mature: 467

Protein sequence:

>467_residues
MRLYNTMTRTKEEFKPLKDKVVNMYVCGPTVYNYIHIGNARAFIVFDTVRRYLEYKGYTVNYVQNFTDIDDKIINRAKEE
NTTEKEIAERFIEEYFKDADALGIKRATVHPRATEHIDDIIEFIKILIDKGYAYVVDGNVYFETAKFKDYGKLSHKNIDE
LMAGARVEIDEKKKNPLDFALWKAQKPGEPAWDSPWGKGRPGWHIECSVMSTKYLGKTLDIHAGGPDLIFPHHENEIAQS
EAAYDQPFSKYWMHIGYLNVNNEKMSKSKGNFFTVRELTEKYDPEVLRLFMLMAHYRSPINFSLDLLDQAKSAYERLSNA
VVNLKHLANITKDRELSDEEKDLIVKFDEYKKQFEEAMDDDFNTADAISVLFEMAKTANANLSGNSSKKLVEYILEMFLK
LSDVLGLSYKNAETEIEDEEILRLIEERQKARKEKNWKLADEIRDKLKERGIILEDTPEGVRWKRIR

Sequences:

>Translated_467_residues
MRLYNTMTRTKEEFKPLKDKVVNMYVCGPTVYNYIHIGNARAFIVFDTVRRYLEYKGYTVNYVQNFTDIDDKIINRAKEE
NTTEKEIAERFIEEYFKDADALGIKRATVHPRATEHIDDIIEFIKILIDKGYAYVVDGNVYFETAKFKDYGKLSHKNIDE
LMAGARVEIDEKKKNPLDFALWKAQKPGEPAWDSPWGKGRPGWHIECSVMSTKYLGKTLDIHAGGPDLIFPHHENEIAQS
EAAYDQPFSKYWMHIGYLNVNNEKMSKSKGNFFTVRELTEKYDPEVLRLFMLMAHYRSPINFSLDLLDQAKSAYERLSNA
VVNLKHLANITKDRELSDEEKDLIVKFDEYKKQFEEAMDDDFNTADAISVLFEMAKTANANLSGNSSKKLVEYILEMFLK
LSDVLGLSYKNAETEIEDEEILRLIEERQKARKEKNWKLADEIRDKLKERGIILEDTPEGVRWKRIR
>Mature_467_residues
MRLYNTMTRTKEEFKPLKDKVVNMYVCGPTVYNYIHIGNARAFIVFDTVRRYLEYKGYTVNYVQNFTDIDDKIINRAKEE
NTTEKEIAERFIEEYFKDADALGIKRATVHPRATEHIDDIIEFIKILIDKGYAYVVDGNVYFETAKFKDYGKLSHKNIDE
LMAGARVEIDEKKKNPLDFALWKAQKPGEPAWDSPWGKGRPGWHIECSVMSTKYLGKTLDIHAGGPDLIFPHHENEIAQS
EAAYDQPFSKYWMHIGYLNVNNEKMSKSKGNFFTVRELTEKYDPEVLRLFMLMAHYRSPINFSLDLLDQAKSAYERLSNA
VVNLKHLANITKDRELSDEEKDLIVKFDEYKKQFEEAMDDDFNTADAISVLFEMAKTANANLSGNSSKKLVEYILEMFLK
LSDVLGLSYKNAETEIEDEEILRLIEERQKARKEKNWKLADEIRDKLKERGIILEDTPEGVRWKRIR

Specific function: Unknown

COG id: COG0215

COG function: function code J; Cysteinyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI13375695, Length=481, Percent_Identity=35.3430353430353, Blast_Score=269, Evalue=5e-72,
Organism=Homo sapiens, GI21269877, Length=438, Percent_Identity=32.1917808219178, Blast_Score=215, Evalue=7e-56,
Organism=Homo sapiens, GI303304987, Length=438, Percent_Identity=32.1917808219178, Blast_Score=215, Evalue=9e-56,
Organism=Homo sapiens, GI62240992, Length=410, Percent_Identity=33.6585365853659, Blast_Score=214, Evalue=1e-55,
Organism=Homo sapiens, GI10835051, Length=410, Percent_Identity=33.6585365853659, Blast_Score=214, Evalue=1e-55,
Organism=Escherichia coli, GI1786737, Length=476, Percent_Identity=48.109243697479, Blast_Score=429, Evalue=1e-121,
Organism=Caenorhabditis elegans, GI17509797, Length=323, Percent_Identity=37.1517027863777, Blast_Score=202, Evalue=2e-52,
Organism=Caenorhabditis elegans, GI17509795, Length=323, Percent_Identity=37.1517027863777, Blast_Score=202, Evalue=2e-52,
Organism=Saccharomyces cerevisiae, GI6324082, Length=331, Percent_Identity=36.8580060422961, Blast_Score=224, Evalue=2e-59,
Organism=Drosophila melanogaster, GI19922218, Length=501, Percent_Identity=34.1317365269461, Blast_Score=268, Evalue=8e-72,
Organism=Drosophila melanogaster, GI19922364, Length=325, Percent_Identity=36, Blast_Score=201, Evalue=1e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYC_THETN (Q8R7T3)

Other databases:

- EMBL:   AE008691
- RefSeq:   NP_623852.1
- ProteinModelPortal:   Q8R7T3
- SMR:   Q8R7T3
- GeneID:   996160
- GenomeReviews:   AE008691_GR
- KEGG:   tte:TTE2315
- NMPDR:   fig|273068.3.peg.2493
- HOGENOM:   HBG327651
- OMA:   DFDALNM
- ProtClustDB:   PRK00260
- BioCyc:   TTEN273068:TTE2315-MONOMER
- BRENDA:   6.1.1.16
- GO:   GO:0005737
- HAMAP:   MF_00041_B
- InterPro:   IPR015803
- InterPro:   IPR015273
- InterPro:   IPR014729
- InterPro:   IPR009080
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR10890
- PRINTS:   PR00983
- SMART:   SM00840
- TIGRFAMs:   TIGR00435

Pfam domain/function: PF09190 DALR_2; PF01406 tRNA-synt_1e; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.16

Molecular weight: Translated: 54524; Mature: 54524

Theoretical pI: Translated: 5.88; Mature: 5.88

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 267-267

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLYNTMTRTKEEFKPLKDKVVNMYVCGPTVYNYIHIGNARAFIVFDTVRRYLEYKGYTV
CCCHHHHHHHHHHHHHHHHHHEEEEEECHHHEEEEEECCCEEEEEHHHHHHHHHHCCCEE
NYVQNFTDIDDKIINRAKEENTTEKEIAERFIEEYFKDADALGIKRATVHPRATEHIDDI
EHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHCCCEEECCCCCHHHHHHHH
IEFIKILIDKGYAYVVDGNVYFETAKFKDYGKLSHKNIDELMAGARVEIDEKKKNPLDFA
HHHHHHHHHCCCEEEECCEEEEEECCCCCCCCCCCCCHHHHHCCCCEEECCCCCCCCCEE
LWKAQKPGEPAWDSPWGKGRPGWHIECSVMSTKYLGKTLDIHAGGPDLIFPHHENEIAQS
EECCCCCCCCCCCCCCCCCCCCCEEEEEEECHHHCCCEEEEECCCCCEECCCCCCHHHHH
EAAYDQPFSKYWMHIGYLNVNNEKMSKSKGNFFTVRELTEKYDPEVLRLFMLMAHYRSPI
HHHHCCCHHHHHHEEEEEEECCHHHHCCCCCEEEHHHHHHHCCHHHHHHHHHHHHHCCCC
NFSLDLLDQAKSAYERLSNAVVNLKHLANITKDRELSDEEKDLIVKFDEYKKQFEEAMDD
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHEEEHHHHHHHHHHHHCC
DFNTADAISVLFEMAKTANANLSGNSSKKLVEYILEMFLKLSDVLGLSYKNAETEIEDEE
CCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHH
ILRLIEERQKARKEKNWKLADEIRDKLKERGIILEDTPEGVRWKRIR
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCEEEECCCCCCCEECCC
>Mature Secondary Structure
MRLYNTMTRTKEEFKPLKDKVVNMYVCGPTVYNYIHIGNARAFIVFDTVRRYLEYKGYTV
CCCHHHHHHHHHHHHHHHHHHEEEEEECHHHEEEEEECCCEEEEEHHHHHHHHHHCCCEE
NYVQNFTDIDDKIINRAKEENTTEKEIAERFIEEYFKDADALGIKRATVHPRATEHIDDI
EHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHCCCEEECCCCCHHHHHHHH
IEFIKILIDKGYAYVVDGNVYFETAKFKDYGKLSHKNIDELMAGARVEIDEKKKNPLDFA
HHHHHHHHHCCCEEEECCEEEEEECCCCCCCCCCCCCHHHHHCCCCEEECCCCCCCCCEE
LWKAQKPGEPAWDSPWGKGRPGWHIECSVMSTKYLGKTLDIHAGGPDLIFPHHENEIAQS
EECCCCCCCCCCCCCCCCCCCCCEEEEEEECHHHCCCEEEEECCCCCEECCCCCCHHHHH
EAAYDQPFSKYWMHIGYLNVNNEKMSKSKGNFFTVRELTEKYDPEVLRLFMLMAHYRSPI
HHHHCCCHHHHHHEEEEEEECCHHHHCCCCCEEEHHHHHHHCCHHHHHHHHHHHHHCCCC
NFSLDLLDQAKSAYERLSNAVVNLKHLANITKDRELSDEEKDLIVKFDEYKKQFEEAMDD
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHEEEHHHHHHHHHHHHCC
DFNTADAISVLFEMAKTANANLSGNSSKKLVEYILEMFLKLSDVLGLSYKNAETEIEDEE
CCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHH
ILRLIEERQKARKEKNWKLADEIRDKLKERGIILEDTPEGVRWKRIR
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCEEEECCCCCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11997336