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The map label for this gene is XthA [H]

Identifier: 20808602

GI number: 20808602

Start: 2132658

End: 2133434

Strand: Direct

Name: XthA [H]

Synonym: TTE2226

Alternate gene names: 20808602

Gene position: 2132658-2133434 (Clockwise)

Preceding gene: 20808596

Following gene: 20808616

Centisome position: 79.3

GC content: 38.48

Gene sequence:

>777_bases
ATGAAACTCGTATCGTGGAATGTAAACGGATTAAGAGCTTGTCTGCAAAAAGGCTTTATGGATTACTTTAAGGCGATAGA
CGCGGATATCTTCTGCATACAAGAGACGAAGCTTCAAGAAAACCAAAAAGACATTGAGGGGCTAGACCTTAATGGGTATT
ATGCCTTCTGGAATTTTGCAGAGAAAAAAGGCTATTCTGGCACAGCAGTATTCACAAAATATAAGCCTCTTTCCGTATCA
TACGGCATAGGAACCCCGCACCACGATAAAGAAGGAAGAGTAATAACACTGGAATACAAAAAGTTCTTTTTGGTAAACGC
TTATACACCTAATTCCCAGAGAGGACTCACAAGGCTTAATTACAGAATGGAGTGGGAAGAGGATTTCCGAAGTTATCTTC
TTAAGTTAGACTCTGTAAAACCAGTAATTTTGTGTGGAGACCTCAATGTTGCTCATCAGGAGATAGACATAAAAAATCCT
GCTGCAAATAGGCGAAATGCAGGATTTACAGATGAAGAAAGAGAAAAAATGACCATTTTACTAAATTCCGGCTTCATAGA
TACTTTCAGATATTTCTATCCTGACAAAAAGGACGCTTATACCTGGTGGTCTTATATGCACAATGCCAGGGAAAAAAACA
TAGGATGGAGAGTCGACTATTTCATCGTATCAGAGAGATTAAAAGACTACCTTATAGACTCTCAAATCCACTCTGAAGTA
ATGGGAAGTGACCACTGCCCTGTAGTTCTTATAGTAAAAGAAGACCTCATAAAATAA

Upstream 100 bases:

>100_bases
ATTTGTAAATAGTTTTTATTGGAAAAGCACATAATTTTGCTTTTGTATCATTTTCATTATATAATGTAAATAGCATTTTA
ATGATAACGGAGGAACTCAT

Downstream 100 bases:

>100_bases
AAGCCCGAAAAGGGCTTTTTAATATTCATCTCTTCTTATTCCGAACTCTGCTGACTCTACCCATTGTTCTGCTTTTCTTA
AATGTCCAATTAGGTTATCA

Product: exonuclease III

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 258; Mature: 258

Protein sequence:

>258_residues
MKLVSWNVNGLRACLQKGFMDYFKAIDADIFCIQETKLQENQKDIEGLDLNGYYAFWNFAEKKGYSGTAVFTKYKPLSVS
YGIGTPHHDKEGRVITLEYKKFFLVNAYTPNSQRGLTRLNYRMEWEEDFRSYLLKLDSVKPVILCGDLNVAHQEIDIKNP
AANRRNAGFTDEEREKMTILLNSGFIDTFRYFYPDKKDAYTWWSYMHNAREKNIGWRVDYFIVSERLKDYLIDSQIHSEV
MGSDHCPVVLIVKEDLIK

Sequences:

>Translated_258_residues
MKLVSWNVNGLRACLQKGFMDYFKAIDADIFCIQETKLQENQKDIEGLDLNGYYAFWNFAEKKGYSGTAVFTKYKPLSVS
YGIGTPHHDKEGRVITLEYKKFFLVNAYTPNSQRGLTRLNYRMEWEEDFRSYLLKLDSVKPVILCGDLNVAHQEIDIKNP
AANRRNAGFTDEEREKMTILLNSGFIDTFRYFYPDKKDAYTWWSYMHNAREKNIGWRVDYFIVSERLKDYLIDSQIHSEV
MGSDHCPVVLIVKEDLIK
>Mature_258_residues
MKLVSWNVNGLRACLQKGFMDYFKAIDADIFCIQETKLQENQKDIEGLDLNGYYAFWNFAEKKGYSGTAVFTKYKPLSVS
YGIGTPHHDKEGRVITLEYKKFFLVNAYTPNSQRGLTRLNYRMEWEEDFRSYLLKLDSVKPVILCGDLNVAHQEIDIKNP
AANRRNAGFTDEEREKMTILLNSGFIDTFRYFYPDKKDAYTWWSYMHNAREKNIGWRVDYFIVSERLKDYLIDSQIHSEV
MGSDHCPVVLIVKEDLIK

Specific function: Major Apurinic-Apyrimidinic Endonuclease Of E.Coli. It Removes The Damaged DNA At Cytosines And Guanines By Cleaving On The 3' Side Of The Ap Site By A Beta-Elimination Reaction. It Exhibits 3'-5'-Exonuclease, 3'-Phosphomonoesterase, 3'-Repair Diesterase

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=253, Percent_Identity=50.9881422924901, Blast_Score=276, Evalue=2e-74,
Organism=Homo sapiens, GI18375503, Length=253, Percent_Identity=50.9881422924901, Blast_Score=276, Evalue=2e-74,
Organism=Homo sapiens, GI18375501, Length=253, Percent_Identity=50.9881422924901, Blast_Score=276, Evalue=2e-74,
Organism=Homo sapiens, GI18375507, Length=313, Percent_Identity=29.3929712460064, Blast_Score=107, Evalue=9e-24,
Organism=Escherichia coli, GI1788046, Length=265, Percent_Identity=28.6792452830189, Blast_Score=116, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI71989536, Length=253, Percent_Identity=44.2687747035573, Blast_Score=216, Evalue=7e-57,
Organism=Caenorhabditis elegans, GI71989539, Length=143, Percent_Identity=37.0629370629371, Blast_Score=94, Evalue=5e-20,
Organism=Drosophila melanogaster, GI221330655, Length=250, Percent_Identity=50.8, Blast_Score=287, Evalue=5e-78,
Organism=Drosophila melanogaster, GI17136678, Length=250, Percent_Identity=50.8, Blast_Score=286, Evalue=7e-78,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 30274; Mature: 30274

Theoretical pI: Translated: 7.11; Mature: 7.11

Prosite motif: PS00726 AP_NUCLEASE_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLVSWNVNGLRACLQKGFMDYFKAIDADIFCIQETKLQENQKDIEGLDLNGYYAFWNFA
CEEEEECHHHHHHHHHHHHHHHHHHHCCEEEEEEHHHHHCCCCCCCCCCCCCEEEEEEEH
EKKGYSGTAVFTKYKPLSVSYGIGTPHHDKEGRVITLEYKKFFLVNAYTPNSQRGLTRLN
HHCCCCCCEEEEEECCEEEEECCCCCCCCCCCCEEEEEEEEEEEEEEECCCCCCCCEEEE
YRMEWEEDFRSYLLKLDSVKPVILCGDLNVAHQEIDIKNPAANRRNAGFTDEEREKMTIL
EEECHHHHHHHHHHHHCCCCCEEEECCCCCCHHEEECCCCCCCCCCCCCCCCCCCEEEEE
LNSGFIDTFRYFYPDKKDAYTWWSYMHNAREKNIGWRVDYFIVSERLKDYLIDSQIHSEV
EECCCHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHH
MGSDHCPVVLIVKEDLIK
HCCCCCCEEEEECCCCCC
>Mature Secondary Structure
MKLVSWNVNGLRACLQKGFMDYFKAIDADIFCIQETKLQENQKDIEGLDLNGYYAFWNFA
CEEEEECHHHHHHHHHHHHHHHHHHHCCEEEEEEHHHHHCCCCCCCCCCCCCEEEEEEEH
EKKGYSGTAVFTKYKPLSVSYGIGTPHHDKEGRVITLEYKKFFLVNAYTPNSQRGLTRLN
HHCCCCCCEEEEEECCEEEEECCCCCCCCCCCCEEEEEEEEEEEEEEECCCCCCCCEEEE
YRMEWEEDFRSYLLKLDSVKPVILCGDLNVAHQEIDIKNPAANRRNAGFTDEEREKMTIL
EEECHHHHHHHHHHHHCCCCCEEEECCCCCCHHEEECCCCCCCCCCCCCCCCCCCEEEEE
LNSGFIDTFRYFYPDKKDAYTWWSYMHNAREKNIGWRVDYFIVSERLKDYLIDSQIHSEV
EECCCHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHH
MGSDHCPVVLIVKEDLIK
HCCCCCCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]