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The map label for this gene is MazF [H]

Identifier: 20808550

GI number: 20808550

Start: 2087150

End: 2087506

Strand: Reverse

Name: MazF [H]

Synonym: TTE2166

Alternate gene names: 20808550

Gene position: 2087506-2087150 (Counterclockwise)

Preceding gene: 20808551

Following gene: 20808549

Centisome position: 77.62

GC content: 38.94

Gene sequence:

>357_bases
TTGATTATGGTAGTGAAAAGAGGAGACTTGTTTTATGCCGATTTAAGCCCTGTGATAGGCTCTGAACAGGGTGGAATTCG
GCCTGTACTTATAATTCAAAATGATATAGGGAATAAGTATAGTCCCACTGTGATCGTAGCGGCAATAACATCACAGATCA
ATAAAGCAAAGCTGCCCACCCATGTAGAGATAAATGGGGCTGAATACGGCCTTCAGAAAGATTCTGTGATTCTTTTGGAA
CAGATAAGGACTATTGATAAAAAGCGTTTGAGGGAGAAAATAGGACATCTTGATCAGGAAATGATGGAAAAGGTCAATGA
GGCTTTACAAATAAGCTTGGGATTGATTGATTTTTAG

Upstream 100 bases:

>100_bases
ATATGGAGATGGCAGCTATAAATTCTGAACTTGCGGAAATGGGCCTAACCGCAGAAAATGAATGTTTTGCAGGTTATGAA
ATGAAACTGAAAAAGTGTGA

Downstream 100 bases:

>100_bases
GGGAACTTGCTCCATTCAAGTTCCTCTTTTTTTGTTGTTGTGATATAATATGAATGGTTATGTATTTATCCTTTCCGTTA
AGAGAAAAGGGGAGTGGAAA

Product: growth inhibitor

Products: NA

Alternate protein names: Endoribonuclease EndoA; Toxin EndoA [H]

Number of amino acids: Translated: 118; Mature: 118

Protein sequence:

>118_residues
MIMVVKRGDLFYADLSPVIGSEQGGIRPVLIIQNDIGNKYSPTVIVAAITSQINKAKLPTHVEINGAEYGLQKDSVILLE
QIRTIDKKRLREKIGHLDQEMMEKVNEALQISLGLIDF

Sequences:

>Translated_118_residues
MIMVVKRGDLFYADLSPVIGSEQGGIRPVLIIQNDIGNKYSPTVIVAAITSQINKAKLPTHVEINGAEYGLQKDSVILLE
QIRTIDKKRLREKIGHLDQEMMEKVNEALQISLGLIDF
>Mature_118_residues
MIMVVKRGDLFYADLSPVIGSEQGGIRPVLIIQNDIGNKYSPTVIVAAITSQINKAKLPTHVEINGAEYGLQKDSVILLE
QIRTIDKKRLREKIGHLDQEMMEKVNEALQISLGLIDF

Specific function: Part of a toxin-antitoxin (TA) module. Has specificity for UAC sequences, yielding cleavage products with 3' phosphate and 5' hydroxyl groups. Overexpression is toxic for cell growth, probably by inhibiting protein synthesis through the cleavage of single

COG id: COG2337

COG function: function code T; Growth inhibitor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PemK/MazF family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003477
- InterPro:   IPR011067 [H]

Pfam domain/function: PF02452 PemK [H]

EC number: NA

Molecular weight: Translated: 13158; Mature: 13158

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIMVVKRGDLFYADLSPVIGSEQGGIRPVLIIQNDIGNKYSPTVIVAAITSQINKAKLPT
CEEEEECCCEEEEECCCCCCCCCCCCCEEEEEECCCCCCCCCEEEEEEHHHHHHHCCCCE
HVEINGAEYGLQKDSVILLEQIRTIDKKRLREKIGHLDQEMMEKVNEALQISLGLIDF
EEEECCHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHEECCCEECC
>Mature Secondary Structure
MIMVVKRGDLFYADLSPVIGSEQGGIRPVLIIQNDIGNKYSPTVIVAAITSQINKAKLPT
CEEEEECCCEEEEECCCCCCCCCCCCCEEEEEECCCCCCCCCEEEEEEHHHHHHHCCCCE
HVEINGAEYGLQKDSVILLEQIRTIDKKRLREKIGHLDQEMMEKVNEALQISLGLIDF
EEEECCHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHEECCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]