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The map label for this gene is MET17 [H]

Identifier: 20808539

GI number: 20808539

Start: 2073908

End: 2075191

Strand: Reverse

Name: MET17 [H]

Synonym: TTE2151

Alternate gene names: 20808539

Gene position: 2075191-2073908 (Counterclockwise)

Preceding gene: 20808540

Following gene: 20808538

Centisome position: 77.16

GC content: 42.29

Gene sequence:

>1284_bases
ATGAGGAGAAAATACGGTTTTTCTACTATAGCAATTCATGAGGGATGGGAACCTGACCCTACTACTGGTGCAGTAGCGCT
GCCAATCTACCAAACTACCTCCTTTGCCTTTAAGAGCACGCAGCATGCAGCAGACTTATTTTCTCTAAAGGAAGACGGAT
ATATTTACACGAGGATGATGAATCCCACTGTAGACGCTTTTGAAAGGAAAATGGCTGCATTAGAAGGGGGAGTAGGAGCG
CTGGCAGTAGCTTCAGGGCAGGCTGCTATAACTATAGCTGTTACAAATATTGCAGGGGCTGGAGATGAGATTGTAGCTTC
TACGAATCTCTACGGCGGGACTTATAACCTTTTTGCCTCTACCCTTAAGAAATTTGGTATAAATGTGAAGTTTGCAGATC
CAAGTGACCCTGAAAATTTTAGAGAAAAAATAACGGAGAAGACCAAAGCTTTATACGTAGAGACAATTGGCAATCCCAAA
ATTGATGTGGCTGATATAGAAAAAATAGCATCAGTTGCTCACAATGCAGGTATACCCCTTATTGTAGATAATACTTTTGC
AACTCCATATCTTTCAAGGCCTATAGAATTTGGGGCAGACATAGTAGTCCACTCAACCACAAAGTTTATAGGAGGGCATG
GTACTTCTATCGGAGGTGTAATTGTGGACTCTGGAAAATTTAATTGGGCGAATGGCAAGTTTCCAGAGCTTACAGAACCT
GACCCCTCTTACCACGGGATAAGGTATGTGAAAGAGTTTGGAGAGAGGGCTTATATAGTCAAAGCGAGAGTGCAGCTTTT
AAGAGACTTGGGGGCGGCTCTTTCGCCTTTTAACGCTTTCTTGTTCGTTCAAGGGCTTGAGACACTTGCACTCAGAATGG
AAAGGCATAGTTATAATGCACTTCGCGTGGCAGAATTTCTGGCTTCTCATCCTCTTGTGAGCTGGGTAAACTATCCTGGC
CTTAAGTCAAGCCCTTATTATGAGCTTGCTCAAAAATATCTTCCAAAAGGGCAGGGTGGAGTTTTGACTTTTGGAATAAA
AGGAGGCTTTGAGGCAGGGGTAAAGTTTATAGAGAACTTAAGATTATTCACCCATCTTGCAAATATAGGAGATGTAAGGT
CTTTGGTCATACATCCTGCTAGCACCACCCATCAGCAGCTTTCAAAAGAAGAACAATTGGCTTCAGGCGTTACTGAGGAT
ATGATTAGGCTTTCTATTGGCCTTGAAGATATAGAGGATATTCTTGAAGACCTGGATTTTGCACTTTACAGTTCTCAAAA
GTAA

Upstream 100 bases:

>100_bases
GCTGTTTTTAAGGTTTTGCCTGAAGAAAATTTAAAAGAGAGAAGCGAGTTTGAAAACTTGGTAGAAGTAATGTAAAAATT
TAGGAAAGAGGAGAGGAAGA

Downstream 100 bases:

>100_bases
GGGGGGATGGGAATGATAGTAGAAGAGAAAAAAGTTACTTTAAAAGGGGATCCTGTTTTTACTACGGAAAGCGGTTATGG
CTTTAAAGAGCTTACAATTG

Product: O-acetylhomoserine sulfhydrylase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 427; Mature: 427

Protein sequence:

>427_residues
MRRKYGFSTIAIHEGWEPDPTTGAVALPIYQTTSFAFKSTQHAADLFSLKEDGYIYTRMMNPTVDAFERKMAALEGGVGA
LAVASGQAAITIAVTNIAGAGDEIVASTNLYGGTYNLFASTLKKFGINVKFADPSDPENFREKITEKTKALYVETIGNPK
IDVADIEKIASVAHNAGIPLIVDNTFATPYLSRPIEFGADIVVHSTTKFIGGHGTSIGGVIVDSGKFNWANGKFPELTEP
DPSYHGIRYVKEFGERAYIVKARVQLLRDLGAALSPFNAFLFVQGLETLALRMERHSYNALRVAEFLASHPLVSWVNYPG
LKSSPYYELAQKYLPKGQGGVLTFGIKGGFEAGVKFIENLRLFTHLANIGDVRSLVIHPASTTHQQLSKEEQLASGVTED
MIRLSIGLEDIEDILEDLDFALYSSQK

Sequences:

>Translated_427_residues
MRRKYGFSTIAIHEGWEPDPTTGAVALPIYQTTSFAFKSTQHAADLFSLKEDGYIYTRMMNPTVDAFERKMAALEGGVGA
LAVASGQAAITIAVTNIAGAGDEIVASTNLYGGTYNLFASTLKKFGINVKFADPSDPENFREKITEKTKALYVETIGNPK
IDVADIEKIASVAHNAGIPLIVDNTFATPYLSRPIEFGADIVVHSTTKFIGGHGTSIGGVIVDSGKFNWANGKFPELTEP
DPSYHGIRYVKEFGERAYIVKARVQLLRDLGAALSPFNAFLFVQGLETLALRMERHSYNALRVAEFLASHPLVSWVNYPG
LKSSPYYELAQKYLPKGQGGVLTFGIKGGFEAGVKFIENLRLFTHLANIGDVRSLVIHPASTTHQQLSKEEQLASGVTED
MIRLSIGLEDIEDILEDLDFALYSSQK
>Mature_427_residues
MRRKYGFSTIAIHEGWEPDPTTGAVALPIYQTTSFAFKSTQHAADLFSLKEDGYIYTRMMNPTVDAFERKMAALEGGVGA
LAVASGQAAITIAVTNIAGAGDEIVASTNLYGGTYNLFASTLKKFGINVKFADPSDPENFREKITEKTKALYVETIGNPK
IDVADIEKIASVAHNAGIPLIVDNTFATPYLSRPIEFGADIVVHSTTKFIGGHGTSIGGVIVDSGKFNWANGKFPELTEP
DPSYHGIRYVKEFGERAYIVKARVQLLRDLGAALSPFNAFLFVQGLETLALRMERHSYNALRVAEFLASHPLVSWVNYPG
LKSSPYYELAQKYLPKGQGGVLTFGIKGGFEAGVKFIENLRLFTHLANIGDVRSLVIHPASTTHQQLSKEEQLASGVTED
MIRLSIGLEDIEDILEDLDFALYSSQK

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG2873

COG function: function code E; O-acetylhomoserine sulfhydrylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=405, Percent_Identity=36.5432098765432, Blast_Score=236, Evalue=2e-62,
Organism=Homo sapiens, GI299473759, Length=405, Percent_Identity=33.0864197530864, Blast_Score=192, Evalue=3e-49,
Organism=Homo sapiens, GI25453487, Length=404, Percent_Identity=31.6831683168317, Blast_Score=179, Evalue=4e-45,
Organism=Escherichia coli, GI1790375, Length=427, Percent_Identity=32.3185011709602, Blast_Score=230, Evalue=1e-61,
Organism=Escherichia coli, GI1789383, Length=419, Percent_Identity=25.0596658711217, Blast_Score=143, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI71980708, Length=412, Percent_Identity=33.252427184466, Blast_Score=224, Evalue=9e-59,
Organism=Caenorhabditis elegans, GI17560056, Length=413, Percent_Identity=33.4140435835351, Blast_Score=213, Evalue=2e-55,
Organism=Caenorhabditis elegans, GI17538055, Length=408, Percent_Identity=33.3333333333333, Blast_Score=211, Evalue=7e-55,
Organism=Saccharomyces cerevisiae, GI6323333, Length=427, Percent_Identity=51.288056206089, Blast_Score=398, Evalue=1e-112,
Organism=Saccharomyces cerevisiae, GI6319307, Length=423, Percent_Identity=37.3522458628842, Blast_Score=234, Evalue=3e-62,
Organism=Saccharomyces cerevisiae, GI6321254, Length=439, Percent_Identity=26.6514806378132, Blast_Score=135, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6321170, Length=363, Percent_Identity=28.6501377410468, Blast_Score=126, Evalue=6e-30,
Organism=Saccharomyces cerevisiae, GI6321904, Length=429, Percent_Identity=25.8741258741259, Blast_Score=105, Evalue=2e-23,
Organism=Drosophila melanogaster, GI19922554, Length=426, Percent_Identity=34.5070422535211, Blast_Score=233, Evalue=1e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 46643; Mature: 46643

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRKYGFSTIAIHEGWEPDPTTGAVALPIYQTTSFAFKSTQHAADLFSLKEDGYIYTRMM
CCCCCCCEEEEEECCCCCCCCCCEEEEEEEECCHHHHHHHHHHHHHHHCCCCCEEEEEEC
NPTVDAFERKMAALEGGVGALAVASGQAAITIAVTNIAGAGDEIVASTNLYGGTYNLFAS
CCCHHHHHHHHHHHCCCCCEEEEECCCEEEEEEEEECCCCCCCEEEECCCCCCHHHHHHH
TLKKFGINVKFADPSDPENFREKITEKTKALYVETIGNPKIDVADIEKIASVAHNAGIPL
HHHHHCCEEEECCCCCHHHHHHHHHHHHHEEEEEECCCCCCCHHHHHHHHHHHHCCCCCE
IVDNTFATPYLSRPIEFGADIVVHSTTKFIGGHGTSIGGVIVDSGKFNWANGKFPELTEP
EEECCCCCCHHCCCHHCCCCEEEEECHHHCCCCCCCCCCEEEECCEEECCCCCCCCCCCC
DPSYHGIRYVKEFGERAYIVKARVQLLRDLGAALSPFNAFLFVQGLETLALRMERHSYNA
CCCCHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCH
LRVAEFLASHPLVSWVNYPGLKSSPYYELAQKYLPKGQGGVLTFGIKGGFEAGVKFIENL
HHHHHHHHHCCHHHHHCCCCCCCCCHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHH
RLFTHLANIGDVRSLVIHPASTTHQQLSKEEQLASGVTEDMIRLSIGLEDIEDILEDLDF
HHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHH
ALYSSQK
HHHCCCC
>Mature Secondary Structure
MRRKYGFSTIAIHEGWEPDPTTGAVALPIYQTTSFAFKSTQHAADLFSLKEDGYIYTRMM
CCCCCCCEEEEEECCCCCCCCCCEEEEEEEECCHHHHHHHHHHHHHHHCCCCCEEEEEEC
NPTVDAFERKMAALEGGVGALAVASGQAAITIAVTNIAGAGDEIVASTNLYGGTYNLFAS
CCCHHHHHHHHHHHCCCCCEEEEECCCEEEEEEEEECCCCCCCEEEECCCCCCHHHHHHH
TLKKFGINVKFADPSDPENFREKITEKTKALYVETIGNPKIDVADIEKIASVAHNAGIPL
HHHHHCCEEEECCCCCHHHHHHHHHHHHHEEEEEECCCCCCCHHHHHHHHHHHHCCCCCE
IVDNTFATPYLSRPIEFGADIVVHSTTKFIGGHGTSIGGVIVDSGKFNWANGKFPELTEP
EEECCCCCCHHCCCHHCCCCEEEEECHHHCCCCCCCCCCEEEECCEEECCCCCCCCCCCC
DPSYHGIRYVKEFGERAYIVKARVQLLRDLGAALSPFNAFLFVQGLETLALRMERHSYNA
CCCCHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCH
LRVAEFLASHPLVSWVNYPGLKSSPYYELAQKYLPKGQGGVLTFGIKGGFEAGVKFIENL
HHHHHHHHHCCHHHHHCCCCCCCCCHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHH
RLFTHLANIGDVRSLVIHPASTTHQQLSKEEQLASGVTEDMIRLSIGLEDIEDILEDLDF
HHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHH
ALYSSQK
HHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]