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The map label for this gene is hisH

Identifier: 20808525

GI number: 20808525

Start: 2051603

End: 2052214

Strand: Reverse

Name: hisH

Synonym: TTE2135

Alternate gene names: 20808525

Gene position: 2052214-2051603 (Counterclockwise)

Preceding gene: 20808526

Following gene: 20808524

Centisome position: 76.31

GC content: 34.48

Gene sequence:

>612_bases
TTGATAGGCATTATAGATTATGGAATGGGAAATTTGAGAAGCGTTGAAAAAGCTTTAAAATACGTGGGATACGATGCGAA
GATTACAAGTGATGCAAAAGAAATAAAAAATTTTGAGGCCTTGATACTTCCCGGAGTTGGAGCCTTTCCAGATGCCATCA
GGGTTTTAATCAAAACAGGTATGGCTGAAGAGATAAAAATACACATTGAAAAGGGAAAGCCTTTTTTGGGAATATGCTTG
GGAATGCAGCTTTTATTTGAGAAAAGTTTTGAGGGAGGCAAGACAGAGGGGCTTAAAATATTTAAAGGCAATGTTGTAGG
ACTTCCTAAAGGAAATAAAATCCCCCATGTAGGGTGGAACAGTTTGACCATAAAAAAAGACACACCATTTCTTAAAGGAA
TAAAAAATGGAGATTATGTGTATTTTGTCCATTCTTATTATGTAAATGGCAATGAAAAAGATTTTGAATACGCAGTTACT
GAATATGGGATAGAAATTCCAGCTGTCATTATTAAAGACAATGTTTTTTCCTGTCAGTTTCATCCAGAAAAAAGCGGAGA
GACAGGGCTTAAAATTTTAAAAAATTTTGGAGAGATGATAAAATGCTTGTAA

Upstream 100 bases:

>100_bases
AGCTTTGGGTAAAGCGTTGGATGAGGCACTTAAATTGGATGAGAGAATAGATGGCATACCTTCTACTAAAGGGATTTTGT
AAACATAAAGGGGTGGAATT

Downstream 100 bases:

>100_bases
TACCTGCAATTGACATATTGGATGGGAAATGTGTAAGACTCACAAAAGGGAATTTTGATTCAAAAGAAGTATATTATGAC
AATCCTGCAGATATGGCTAA

Product: imidazole glycerol phosphate synthase subunit HisH

Products: NA

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH

Number of amino acids: Translated: 203; Mature: 203

Protein sequence:

>203_residues
MIGIIDYGMGNLRSVEKALKYVGYDAKITSDAKEIKNFEALILPGVGAFPDAIRVLIKTGMAEEIKIHIEKGKPFLGICL
GMQLLFEKSFEGGKTEGLKIFKGNVVGLPKGNKIPHVGWNSLTIKKDTPFLKGIKNGDYVYFVHSYYVNGNEKDFEYAVT
EYGIEIPAVIIKDNVFSCQFHPEKSGETGLKILKNFGEMIKCL

Sequences:

>Translated_203_residues
MIGIIDYGMGNLRSVEKALKYVGYDAKITSDAKEIKNFEALILPGVGAFPDAIRVLIKTGMAEEIKIHIEKGKPFLGICL
GMQLLFEKSFEGGKTEGLKIFKGNVVGLPKGNKIPHVGWNSLTIKKDTPFLKGIKNGDYVYFVHSYYVNGNEKDFEYAVT
EYGIEIPAVIIKDNVFSCQFHPEKSGETGLKILKNFGEMIKCL
>Mature_203_residues
MIGIIDYGMGNLRSVEKALKYVGYDAKITSDAKEIKNFEALILPGVGAFPDAIRVLIKTGMAEEIKIHIEKGKPFLGICL
GMQLLFEKSFEGGKTEGLKIFKGNVVGLPKGNKIPHVGWNSLTIKKDTPFLKGIKNGDYVYFVHSYYVNGNEKDFEYAVT
EYGIEIPAVIIKDNVFSCQFHPEKSGETGLKILKNFGEMIKCL

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Escherichia coli, GI1788334, Length=197, Percent_Identity=38.5786802030457, Blast_Score=128, Evalue=3e-31,
Organism=Saccharomyces cerevisiae, GI6319725, Length=208, Percent_Identity=34.6153846153846, Blast_Score=118, Evalue=6e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS5_THETN (Q8R883)

Other databases:

- EMBL:   AE008691
- RefSeq:   NP_623696.1
- ProteinModelPortal:   Q8R883
- SMR:   Q8R883
- GeneID:   997446
- GenomeReviews:   AE008691_GR
- KEGG:   tte:TTE2135
- NMPDR:   fig|273068.3.peg.2239
- HOGENOM:   HBG292341
- OMA:   RPFFGIC
- ProtClustDB:   PRK13141
- BioCyc:   TTEN273068:TTE2135-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00278
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226
- PIRSF:   PIRSF000495
- TIGRFAMs:   TIGR01855

Pfam domain/function: PF00117 GATase

EC number: 2.4.2.-

Molecular weight: Translated: 22546; Mature: 22546

Theoretical pI: Translated: 8.72; Mature: 8.72

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 79-79 ACT_SITE 181-181 ACT_SITE 183-183

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIGIIDYGMGNLRSVEKALKYVGYDAKITSDAKEIKNFEALILPGVGAFPDAIRVLIKTG
CEEEEECCCCCHHHHHHHHHHCCCCCEECCCHHHHCCCEEEEECCCCCCHHHHHHHHHCC
MAEEIKIHIEKGKPFLGICLGMQLLFEKSFEGGKTEGLKIFKGNVVGLPKGNKIPHVGWN
CCCEEEEEEECCCCEEHHHHHHHHHHHHCCCCCCCCCEEEEECCEEECCCCCCCCCCCCC
SLTIKKDTPFLKGIKNGDYVYFVHSYYVNGNEKDFEYAVTEYGIEIPAVIIKDNVFSCQF
EEEEECCCCHHHCCCCCCEEEEEEEEEECCCCCCCEEEHHHCCCCCCEEEEECCEEEEEE
HPEKSGETGLKILKNFGEMIKCL
CCCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIGIIDYGMGNLRSVEKALKYVGYDAKITSDAKEIKNFEALILPGVGAFPDAIRVLIKTG
CEEEEECCCCCHHHHHHHHHHCCCCCEECCCHHHHCCCEEEEECCCCCCHHHHHHHHHCC
MAEEIKIHIEKGKPFLGICLGMQLLFEKSFEGGKTEGLKIFKGNVVGLPKGNKIPHVGWN
CCCEEEEEEECCCCEEHHHHHHHHHHHHCCCCCCCCCEEEEECCEEECCCCCCCCCCCCC
SLTIKKDTPFLKGIKNGDYVYFVHSYYVNGNEKDFEYAVTEYGIEIPAVIIKDNVFSCQF
EEEEECCCCHHHCCCCCCEEEEEEEEEECCCCCCCEEEHHHCCCCCCEEEEECCEEEEEE
HPEKSGETGLKILKNFGEMIKCL
CCCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11997336