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The map label for this gene is NemA3 [C]

Identifier: 20808521

GI number: 20808521

Start: 2047352

End: 2049295

Strand: Direct

Name: NemA3 [C]

Synonym: TTE2131

Alternate gene names: 20808521

Gene position: 2047352-2049295 (Clockwise)

Preceding gene: 20808520

Following gene: 20808531

Centisome position: 76.13

GC content: 38.12

Gene sequence:

>1944_bases
ATGAGTGAATTCAAGTTTGAAAAGCTTCTAGAACCAATTCAAATTGGGTCAATGAAACTAAGAAATAGGATTATTATGCC
TCCTATGGTTACAAATTACGCTGCTGAGGATGGTGCCGTCACCGAACGTTTTAAAGCTTATCACCAAACCAGAGCAAAAG
GTGGAGTAGGCTTAATTATTGTTGAAGCAACATACATTCATCCAGGTGGTAAAGGCTTTCAAAACCAACTTGGTATTTAC
AAAGATGAATTAATATCTGGTTTGAAAGAATTAACAGAGGCAGTTCATAAGTATGGAGCGAAAATTGCTGTGCAACTTTA
TCATGCCGGTAGGCAAACAACTTTGAAAGTTACTGGAATGCAAGTAGTTGCTCCGTCACCTATTCCTTGCCCAGTTAAGC
AAGAAAAGCCCAAAGAATTATCAGTAGACGAAATAAAAGAGTTAATAGAAGCATTTGGACAAGCTGCGAGACGTGCTAAA
GAAGCAGGGTTTGATGCTGTCGAAATCCATGGTGCTCACGGTTATTTAATTAATCAATTCTTATCACCATATAGCAATAA
GCGAACCGATGAATACGGTGGAAGTTTTGAAAACAGAATGAGATTCCCATTGGAAGTTGTCAGGAGAGTAAGAGAAGAAG
TTGGGCCAGATTTTCCTATTATATATCGCATGAGTGCTGAAGAGTATGTTCCTGGAGGACTTACGATTGAAGATACTAAG
ATATTTGCTCAAAAGTTAGTAGAAGAAGGAATCGATGCTTTGCATATTTCGGGTGGAGTATATGAATCTTCAGCAATGAT
TATCCAGCCTGCAGCTATACCTCAGGGTTGTTTTGTAGAAAACGCAGCTGCTATAAAGAAAGCAATTAATAGCAAAGTAC
CTGTAATTGTTGTTGGTCGAATCAAAGATCCTATTATGGCAGAGCAAATCATCCGTGAGGGCAAAGCTGATCTAGTATCA
ATGGGAAGAGCCCTACTAGCAGACCCAGAATTGCCCAGGAAAGTTTCTGAGGGCAAGATTGAAGAAATAAGAAAATGCAT
TGGGTGTAATCAGGGTTGTATTGATCGTCTGTTCCAAGATATTGATATTGGTTGCATAGCCAATGCATTAACTGGACATG
AAACAGAATTTGACATGGAAAGCCTAGCAAAGACAAGAAAGAAAGTATTTATAATCGGTGGTGGCCCTGGCGGGTTAGAA
GCAGCTAGAGTAGCAGCTTTGCGTGGCCATGAAGTAATTCTTTATGAAAAGCAACCAGAATTAGGTGGACAAATGCGAAT
TGCTGCTGTACCGCCTCATAAAGGGGAAATAAATGATCTGGCTGATTACCTCATCAATCAAGTAGAAAAATCTGGTATTA
CGATTGTAAAAGGCAAAGAAGCAGATTTAAACACAATTCATGAAATTAAACCCGATGTGGTTATATTAGCAACAGGGTCT
GAACCAATAATTCCTGAAATTCCTGGAATCAATCAAAAAAATGTTGTTACGGCCCATGATGTTTTAAAAGGGACAGTTAC
TGTTGGTAAAAAAGTAGCTGTCATTGGTGGTGGGTTAGTAGGTTGTGAAACTGCAGAATTCTTGGCTGATCAAGGTAAAG
AAGTAACAGTAATAGAGATGTTGGATGATATAGCAATAGATGTTGGAAGTTTGGTAAGAGCTCTATTACTAAATCGTATG
GCTGAGAAGAAGATTAAAGTATTGACAAAGAGCAAAGTTAAAGAAATATCAGGAGATAAAGTAACTATAGAAACAGTAAA
TGGAAACCAAGAATTAAGTGGTATAGACACTATCGTAATAGCAGTTGGGTCTAAACCCAAGAACGATTTATTGAAGTTAA
TTGAAAAAGAAGGTATACCTGTTTATGCTATTGGGGATTGCGTCAAAGCTAGAAAGTTTATGGACGCTATTCATGAAGGT
TTCCGTTATGCTTACAGCTTGTAA

Upstream 100 bases:

>100_bases
CACCCTCAATATGCATAATTGCTCTTGACATAATGAAAAAATAAAGTTAGCATATTACTTGATAACTGTTAATAAATTAA
CAATATGAGGAGGATTTAAG

Downstream 100 bases:

>100_bases
TTTTAAGTCTTTACAAAAAAGGGGTATCCCAAAATATAGATGGATACCCCTTTTATTATTACTCCAAATTTAATTGTGCT
TATAGCTCATTATAATAACC

Product: NADH:flavin oxidoreductase

Products: trans-transtetradehydroacyl-CoA; NADPH [C]

Alternate protein names: NA

Number of amino acids: Translated: 647; Mature: 646

Protein sequence:

>647_residues
MSEFKFEKLLEPIQIGSMKLRNRIIMPPMVTNYAAEDGAVTERFKAYHQTRAKGGVGLIIVEATYIHPGGKGFQNQLGIY
KDELISGLKELTEAVHKYGAKIAVQLYHAGRQTTLKVTGMQVVAPSPIPCPVKQEKPKELSVDEIKELIEAFGQAARRAK
EAGFDAVEIHGAHGYLINQFLSPYSNKRTDEYGGSFENRMRFPLEVVRRVREEVGPDFPIIYRMSAEEYVPGGLTIEDTK
IFAQKLVEEGIDALHISGGVYESSAMIIQPAAIPQGCFVENAAAIKKAINSKVPVIVVGRIKDPIMAEQIIREGKADLVS
MGRALLADPELPRKVSEGKIEEIRKCIGCNQGCIDRLFQDIDIGCIANALTGHETEFDMESLAKTRKKVFIIGGGPGGLE
AARVAALRGHEVILYEKQPELGGQMRIAAVPPHKGEINDLADYLINQVEKSGITIVKGKEADLNTIHEIKPDVVILATGS
EPIIPEIPGINQKNVVTAHDVLKGTVTVGKKVAVIGGGLVGCETAEFLADQGKEVTVIEMLDDIAIDVGSLVRALLLNRM
AEKKIKVLTKSKVKEISGDKVTIETVNGNQELSGIDTIVIAVGSKPKNDLLKLIEKEGIPVYAIGDCVKARKFMDAIHEG
FRYAYSL

Sequences:

>Translated_647_residues
MSEFKFEKLLEPIQIGSMKLRNRIIMPPMVTNYAAEDGAVTERFKAYHQTRAKGGVGLIIVEATYIHPGGKGFQNQLGIY
KDELISGLKELTEAVHKYGAKIAVQLYHAGRQTTLKVTGMQVVAPSPIPCPVKQEKPKELSVDEIKELIEAFGQAARRAK
EAGFDAVEIHGAHGYLINQFLSPYSNKRTDEYGGSFENRMRFPLEVVRRVREEVGPDFPIIYRMSAEEYVPGGLTIEDTK
IFAQKLVEEGIDALHISGGVYESSAMIIQPAAIPQGCFVENAAAIKKAINSKVPVIVVGRIKDPIMAEQIIREGKADLVS
MGRALLADPELPRKVSEGKIEEIRKCIGCNQGCIDRLFQDIDIGCIANALTGHETEFDMESLAKTRKKVFIIGGGPGGLE
AARVAALRGHEVILYEKQPELGGQMRIAAVPPHKGEINDLADYLINQVEKSGITIVKGKEADLNTIHEIKPDVVILATGS
EPIIPEIPGINQKNVVTAHDVLKGTVTVGKKVAVIGGGLVGCETAEFLADQGKEVTVIEMLDDIAIDVGSLVRALLLNRM
AEKKIKVLTKSKVKEISGDKVTIETVNGNQELSGIDTIVIAVGSKPKNDLLKLIEKEGIPVYAIGDCVKARKFMDAIHEG
FRYAYSL
>Mature_646_residues
SEFKFEKLLEPIQIGSMKLRNRIIMPPMVTNYAAEDGAVTERFKAYHQTRAKGGVGLIIVEATYIHPGGKGFQNQLGIYK
DELISGLKELTEAVHKYGAKIAVQLYHAGRQTTLKVTGMQVVAPSPIPCPVKQEKPKELSVDEIKELIEAFGQAARRAKE
AGFDAVEIHGAHGYLINQFLSPYSNKRTDEYGGSFENRMRFPLEVVRRVREEVGPDFPIIYRMSAEEYVPGGLTIEDTKI
FAQKLVEEGIDALHISGGVYESSAMIIQPAAIPQGCFVENAAAIKKAINSKVPVIVVGRIKDPIMAEQIIREGKADLVSM
GRALLADPELPRKVSEGKIEEIRKCIGCNQGCIDRLFQDIDIGCIANALTGHETEFDMESLAKTRKKVFIIGGGPGGLEA
ARVAALRGHEVILYEKQPELGGQMRIAAVPPHKGEINDLADYLINQVEKSGITIVKGKEADLNTIHEIKPDVVILATGSE
PIIPEIPGINQKNVVTAHDVLKGTVTVGKKVAVIGGGLVGCETAEFLADQGKEVTVIEMLDDIAIDVGSLVRALLLNRMA
EKKIKVLTKSKVKEISGDKVTIETVNGNQELSGIDTIVIAVGSKPKNDLLKLIEKEGIPVYAIGDCVKARKFMDAIHEGF
RYAYSL

Specific function: Reduces a range of alternative electron acceptors [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the N-terminal section; belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1789463, Length=688, Percent_Identity=34.156976744186, Blast_Score=352, Evalue=5e-98,
Organism=Escherichia coli, GI1787939, Length=348, Percent_Identity=35.3448275862069, Blast_Score=162, Evalue=5e-41,
Organism=Escherichia coli, GI1788468, Length=290, Percent_Identity=27.9310344827586, Blast_Score=70, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17559804, Length=306, Percent_Identity=32.6797385620915, Blast_Score=148, Evalue=7e-36,
Organism=Caenorhabditis elegans, GI72001454, Length=257, Percent_Identity=35.408560311284, Blast_Score=146, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI17559802, Length=275, Percent_Identity=34.9090909090909, Blast_Score=143, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI17564188, Length=257, Percent_Identity=34.2412451361868, Blast_Score=142, Evalue=6e-34,
Organism=Caenorhabditis elegans, GI17540738, Length=341, Percent_Identity=30.2052785923754, Blast_Score=136, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI17565138, Length=259, Percent_Identity=33.976833976834, Blast_Score=132, Evalue=7e-31,
Organism=Caenorhabditis elegans, GI17566914, Length=352, Percent_Identity=29.2613636363636, Blast_Score=116, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6325086, Length=354, Percent_Identity=31.9209039548023, Blast_Score=148, Evalue=2e-36,
Organism=Saccharomyces cerevisiae, GI6321973, Length=353, Percent_Identity=31.728045325779, Blast_Score=142, Evalue=1e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR013027
- InterPro:   IPR016040
- InterPro:   IPR001155
- InterPro:   IPR001327
- InterPro:   IPR000103 [H]

Pfam domain/function: PF00724 Oxidored_FMN; PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.3.1.34 [C]

Molecular weight: Translated: 70529; Mature: 70398

Theoretical pI: Translated: 6.71; Mature: 6.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEFKFEKLLEPIQIGSMKLRNRIIMPPMVTNYAAEDGAVTERFKAYHQTRAKGGVGLII
CCCCCHHHHHHHHHCCCHHHCCEEECCCHHHCCCCCCCHHHHHHHHHHHHHCCCCEEEEE
VEATYIHPGGKGFQNQLGIYKDELISGLKELTEAVHKYGAKIAVQLYHAGRQTTLKVTGM
EEEEEECCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCEEEEEECE
QVVAPSPIPCPVKQEKPKELSVDEIKELIEAFGQAARRAKEAGFDAVEIHGAHGYLINQF
EEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHH
LSPYSNKRTDEYGGSFENRMRFPLEVVRRVREEVGPDFPIIYRMSAEEYVPGGLTIEDTK
HCCCCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHCCCCCEEEEECHHHCCCCCCEEEHHH
IFAQKLVEEGIDALHISGGVYESSAMIIQPAAIPQGCFVENAAAIKKAINSKVPVIVVGR
HHHHHHHHCCCCEEEECCCEEECCEEEEECCCCCCCCHHHHHHHHHHHHCCCCCEEEEEC
IKDPIMAEQIIREGKADLVSMGRALLADPELPRKVSEGKIEEIRKCIGCNQGCIDRLFQD
CCCHHHHHHHHHCCCHHHHHHCHHHHCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHH
IDIGCIANALTGHETEFDMESLAKTRKKVFIIGGGPGGLEAARVAALRGHEVILYEKQPE
CCHHHHHHHHCCCCCCCCHHHHHHHCCEEEEEECCCCCHHHHHHHHHCCCEEEEEECCCC
LGGQMRIAAVPPHKGEINDLADYLINQVEKSGITIVKGKEADLNTIHEIKPDVVILATGS
CCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCEEEEECCCCCCCHHHHCCCCEEEEECCC
EPIIPEIPGINQKNVVTAHDVLKGTVTVGKKVAVIGGGLVGCETAEFLADQGKEVTVIEM
CCCCCCCCCCCCCCEEEHHHHHHHHHHCCCEEEEEECCEECCCHHHHHHCCCCCEEHHHH
LDDIAIDVGSLVRALLLNRMAEKKIKVLTKSKVKEISGDKVTIETVNGNQELSGIDTIVI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCEEEEE
AVGSKPKNDLLKLIEKEGIPVYAIGDCVKARKFMDAIHEGFRYAYSL
EECCCCHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SEFKFEKLLEPIQIGSMKLRNRIIMPPMVTNYAAEDGAVTERFKAYHQTRAKGGVGLII
CCCCHHHHHHHHHCCCHHHCCEEECCCHHHCCCCCCCHHHHHHHHHHHHHCCCCEEEEE
VEATYIHPGGKGFQNQLGIYKDELISGLKELTEAVHKYGAKIAVQLYHAGRQTTLKVTGM
EEEEEECCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCEEEEEECE
QVVAPSPIPCPVKQEKPKELSVDEIKELIEAFGQAARRAKEAGFDAVEIHGAHGYLINQF
EEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHH
LSPYSNKRTDEYGGSFENRMRFPLEVVRRVREEVGPDFPIIYRMSAEEYVPGGLTIEDTK
HCCCCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHCCCCCEEEEECHHHCCCCCCEEEHHH
IFAQKLVEEGIDALHISGGVYESSAMIIQPAAIPQGCFVENAAAIKKAINSKVPVIVVGR
HHHHHHHHCCCCEEEECCCEEECCEEEEECCCCCCCCHHHHHHHHHHHHCCCCCEEEEEC
IKDPIMAEQIIREGKADLVSMGRALLADPELPRKVSEGKIEEIRKCIGCNQGCIDRLFQD
CCCHHHHHHHHHCCCHHHHHHCHHHHCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHH
IDIGCIANALTGHETEFDMESLAKTRKKVFIIGGGPGGLEAARVAALRGHEVILYEKQPE
CCHHHHHHHHCCCCCCCCHHHHHHHCCEEEEEECCCCCHHHHHHHHHCCCEEEEEECCCC
LGGQMRIAAVPPHKGEINDLADYLINQVEKSGITIVKGKEADLNTIHEIKPDVVILATGS
CCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCEEEEECCCCCCCHHHHCCCCEEEEECCC
EPIIPEIPGINQKNVVTAHDVLKGTVTVGKKVAVIGGGLVGCETAEFLADQGKEVTVIEM
CCCCCCCCCCCCCCEEEHHHHHHHHHHCCCEEEEEECCEECCCHHHHHHCCCCCEEHHHH
LDDIAIDVGSLVRALLLNRMAEKKIKVLTKSKVKEISGDKVTIETVNGNQELSGIDTIVI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCEEEEE
AVGSKPKNDLLKLIEKEGIPVYAIGDCVKARKFMDAIHEGFRYAYSL
EECCCCHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: transDidehydroacyl-CoA; NADP+ [C]

Specific reaction: transDidehydroacyl-CoA + NADP+ =trans-transtetradehydroacyl-CoA + NADPH [C]

General reaction: Redox reaction [C]

Inhibitor: Iodoacetic acid; N-Ethyl maleimide [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8357835 [H]