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The map label for this gene is HcaD2 [H]

Identifier: 20808403

GI number: 20808403

Start: 1948323

End: 1949582

Strand: Reverse

Name: HcaD2 [H]

Synonym: TTE2000

Alternate gene names: 20808403

Gene position: 1949582-1948323 (Counterclockwise)

Preceding gene: 20808404

Following gene: 20808402

Centisome position: 72.49

GC content: 40.87

Gene sequence:

>1260_bases
ATGCTTAACTATGATATAGTCATAATAGGAGGAGGACCTGCAGGCTTAGGTGCTGCTGTAGAAGCCTATGAAAAGGGAGT
AAAAAATGTCGTGATATTGGAAAGAGATAAATACTTGGGCGGCATTTTGCAACAGTGCATACACAATGGATTCGGGCTGC
AAGAATTCAAAGAGGAGTTGACAGGTCCCGAATACGCCCAGAGGTTTATAGACAAAGTAGAAGAATATGGAATAGATGTC
ATGTTGGACACGATGGTTTTAGAGATTACTCCTGACAGAATTGTAAAAGCTGTCAACTCAGAAAAAGGAGTATTCCAAAT
AAAAGCAAAAGCTATAATTCTGGCAATGGGGTGCAGAGAAAGGCCTAGAGGGGCGATAATGATTCCTGGAACCAGACCGG
CAGGAATAATGACAGCAGGAACAGCTCAGAGGTATGTGAATATGGAAGGTTACATGCCAGGGAAAGAGATAGTAATTTTA
GGGTCAGGGGATGTAGGGCTTATCATGGCAAGGAGGCTTACTTTAGAGGGAGCAAAAGTAAAAGCTGTGGTAGAGATAAT
GCCTTATTCAAGCGGCCTTACTAGAAATATAGTGCAATGTCTTGAAGATTTTGATATTCCACTTTTATTGAGCCACACTG
TAGTAGAGATACATGGAAAGGATAGAGTGGAAGGGGTTACTATCGCAAAAGTCGATGAAAACAGAGAACCTATAATGGAA
ACTGCCCAGTATATAAGCTGCGATACTTTGATACTCTCTGTAGGATTGATTCCTGAGAACGAATTGTCCAAACAGGCAGG
GATAGAACTTGATCCAGTGACTGGAGGACCTGTTGTGAATGAGATGCTGGAGACAAGTGTTCCAGGGATTTTTGCTTGTG
GCAATGTCCTTCAGGTTCACGATTTGGTAGACAATGTGACAGCAGAAGCCAGAATGGCAGCTAATTCGGCTATAAGGTTT
ATAAGAGAAGGAGGGTTTAAAAAGAGCAATATCTCTGTTAAAGCGGGGAAAGGAATACGTTATGTAGTGCCACAGAGAAT
AAACGTGGATAATATAGATAAAAGGATAAAATTTAGGATGAGACCGACAGATGTGTACAATAATGCCATATTGGCAGTAA
AGTCGGGTGATAGGTACCTTTTAAGGCAGAAGAGGATAAGGCTTACTCCTGGTGAAATGATAGATGTAAATTTAAGTAAG
GAAATACTAAAAAATGCAGATGAAAAAGAGGGTATTGTCTTTTTGGTGGAGGAGGGATAA

Upstream 100 bases:

>100_bases
GTTACAAAGCATGGCAGGAATTCTTACATTCTTGCCGGTGAGACAAAGAAGTTTTTATTAGACAAGGCTTCAGAGCTTTT
GAAGAAAGAGGTGTATGGCA

Downstream 100 bases:

>100_bases
AATGGAAGTAAAGGAGATTACCTGCATAGTATGTCCTCTGGGCTGCAGGATAAAAGTTGAAATGGAAGGAGGGGAGATAA
AGAGCATAAAAGGCTATTCT

Product: uncharacterized NAD(FAD)-dependent dehydrogenase

Products: NA

Alternate protein names: TRXR [H]

Number of amino acids: Translated: 419; Mature: 419

Protein sequence:

>419_residues
MLNYDIVIIGGGPAGLGAAVEAYEKGVKNVVILERDKYLGGILQQCIHNGFGLQEFKEELTGPEYAQRFIDKVEEYGIDV
MLDTMVLEITPDRIVKAVNSEKGVFQIKAKAIILAMGCRERPRGAIMIPGTRPAGIMTAGTAQRYVNMEGYMPGKEIVIL
GSGDVGLIMARRLTLEGAKVKAVVEIMPYSSGLTRNIVQCLEDFDIPLLLSHTVVEIHGKDRVEGVTIAKVDENREPIME
TAQYISCDTLILSVGLIPENELSKQAGIELDPVTGGPVVNEMLETSVPGIFACGNVLQVHDLVDNVTAEARMAANSAIRF
IREGGFKKSNISVKAGKGIRYVVPQRINVDNIDKRIKFRMRPTDVYNNAILAVKSGDRYLLRQKRIRLTPGEMIDVNLSK
EILKNADEKEGIVFLVEEG

Sequences:

>Translated_419_residues
MLNYDIVIIGGGPAGLGAAVEAYEKGVKNVVILERDKYLGGILQQCIHNGFGLQEFKEELTGPEYAQRFIDKVEEYGIDV
MLDTMVLEITPDRIVKAVNSEKGVFQIKAKAIILAMGCRERPRGAIMIPGTRPAGIMTAGTAQRYVNMEGYMPGKEIVIL
GSGDVGLIMARRLTLEGAKVKAVVEIMPYSSGLTRNIVQCLEDFDIPLLLSHTVVEIHGKDRVEGVTIAKVDENREPIME
TAQYISCDTLILSVGLIPENELSKQAGIELDPVTGGPVVNEMLETSVPGIFACGNVLQVHDLVDNVTAEARMAANSAIRF
IREGGFKKSNISVKAGKGIRYVVPQRINVDNIDKRIKFRMRPTDVYNNAILAVKSGDRYLLRQKRIRLTPGEMIDVNLSK
EILKNADEKEGIVFLVEEG
>Mature_419_residues
MLNYDIVIIGGGPAGLGAAVEAYEKGVKNVVILERDKYLGGILQQCIHNGFGLQEFKEELTGPEYAQRFIDKVEEYGIDV
MLDTMVLEITPDRIVKAVNSEKGVFQIKAKAIILAMGCRERPRGAIMIPGTRPAGIMTAGTAQRYVNMEGYMPGKEIVIL
GSGDVGLIMARRLTLEGAKVKAVVEIMPYSSGLTRNIVQCLEDFDIPLLLSHTVVEIHGKDRVEGVTIAKVDENREPIME
TAQYISCDTLILSVGLIPENELSKQAGIELDPVTGGPVVNEMLETSVPGIFACGNVLQVHDLVDNVTAEARMAANSAIRF
IREGGFKKSNISVKAGKGIRYVVPQRINVDNIDKRIKFRMRPTDVYNNAILAVKSGDRYLLRQKRIRLTPGEMIDVNLSK
EILKNADEKEGIVFLVEEG

Specific function: NITROGEN METABOLISM, GLUTAMATE BIOSYNTHESIS. THE CATALYZED REACTION BRINGS TOGETHER THE NITROGEN AND CARBON METABOLISM. [C]

COG id: COG0446

COG function: function code R; Uncharacterized NAD(FAD)-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI1789606, Length=351, Percent_Identity=25.6410256410256, Blast_Score=74, Evalue=2e-14,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013027
- InterPro:   IPR008255
- InterPro:   IPR001327
- InterPro:   IPR000103
- InterPro:   IPR005982 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.8.1.9 [H]

Molecular weight: Translated: 46013; Mature: 46013

Theoretical pI: Translated: 5.61; Mature: 5.61

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNYDIVIIGGGPAGLGAAVEAYEKGVKNVVILERDKYLGGILQQCIHNGFGLQEFKEEL
CCEEEEEEECCCCCCHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHCCCCHHHHHHHC
TGPEYAQRFIDKVEEYGIDVMLDTMVLEITPDRIVKAVNSEKGVFQIKAKAIILAMGCRE
CCHHHHHHHHHHHHHCCCEEEEEEEEEEECHHHHHHHHCCCCCEEEEEEEEEEEEECCCC
RPRGAIMIPGTRPAGIMTAGTAQRYVNMEGYMPGKEIVILGSGDVGLIMARRLTLEGAKV
CCCCEEEECCCCCCCEEECCCHHHEEECCCCCCCCEEEEEECCCEEEEEEEEEEECCCEE
KAVVEIMPYSSGLTRNIVQCLEDFDIPLLLSHTVVEIHGKDRVEGVTIAKVDENREPIME
EEEEEEECCCCCHHHHHHHHHHHCCCCEEEEEEEEEEECCCCCCCEEEEEECCCCCHHHH
TAQYISCDTLILSVGLIPENELSKQAGIELDPVTGGPVVNEMLETSVPGIFACGNVLQVH
HHHHHHHCEEEEEECCCCCHHHHHHCCCEECCCCCCHHHHHHHHHCCCCEEECCCHHHHH
DLVDNVTAEARMAANSAIRFIREGGFKKSNISVKAGKGIRYVVPQRINVDNIDKRIKFRM
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCEEEECCCCCCCCCCCCEEEEEE
RPTDVYNNAILAVKSGDRYLLRQKRIRLTPGEMIDVNLSKEILKNADEKEGIVFLVEEG
CCCHHCCCEEEEEECCCCEEHHCCCEECCCCCEEEECCHHHHHCCCCCCCCEEEEEECC
>Mature Secondary Structure
MLNYDIVIIGGGPAGLGAAVEAYEKGVKNVVILERDKYLGGILQQCIHNGFGLQEFKEEL
CCEEEEEEECCCCCCHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHCCCCHHHHHHHC
TGPEYAQRFIDKVEEYGIDVMLDTMVLEITPDRIVKAVNSEKGVFQIKAKAIILAMGCRE
CCHHHHHHHHHHHHHCCCEEEEEEEEEEECHHHHHHHHCCCCCEEEEEEEEEEEEECCCC
RPRGAIMIPGTRPAGIMTAGTAQRYVNMEGYMPGKEIVILGSGDVGLIMARRLTLEGAKV
CCCCEEEECCCCCCCEEECCCHHHEEECCCCCCCCEEEEEECCCEEEEEEEEEEECCCEE
KAVVEIMPYSSGLTRNIVQCLEDFDIPLLLSHTVVEIHGKDRVEGVTIAKVDENREPIME
EEEEEEECCCCCHHHHHHHHHHHCCCCEEEEEEEEEEECCCCCCCEEEEEECCCCCHHHH
TAQYISCDTLILSVGLIPENELSKQAGIELDPVTGGPVVNEMLETSVPGIFACGNVLQVH
HHHHHHHCEEEEEECCCCCHHHHHHCCCEECCCCCCHHHHHHHHHCCCCEEECCCHHHHH
DLVDNVTAEARMAANSAIRFIREGGFKKSNISVKAGKGIRYVVPQRINVDNIDKRIKFRM
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCEEEECCCCCCCCCCCCEEEEEE
RPTDVYNNAILAVKSGDRYLLRQKRIRLTPGEMIDVNLSKEILKNADEKEGIVFLVEEG
CCCHHCCCEEEEEECCCCEEHHCCCEECCCCCEEEECCHHHHHCCCCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA