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The map label for this gene is BioB2 [C]

Identifier: 20808294

GI number: 20808294

Start: 1828701

End: 1829762

Strand: Direct

Name: BioB2 [C]

Synonym: TTE1885

Alternate gene names: 20808294

Gene position: 1828701-1829762 (Clockwise)

Preceding gene: 20808228

Following gene: 20808303

Centisome position: 68.0

GC content: 36.82

Gene sequence:

>1062_bases
ATGATAAAAGAACTGACAGCAGAAAAAAATATATCTAGTTTAATCGAAAAATTAGAAAAACAGCATTCTCTTGACAAAAA
AGGCCTTATAATGCTTCTCTCTATGGAAAATCCCTCTGAACTTTATAAAGCTGCAGACAGGGTAAGGAAAAAATATGTAG
GAGATGAAGTGCATTTAAGAGGTCTTATAGAATTTAGCAATTACTGTCACAATACCTGCTTTTACTGTGGATTAAGAGGC
CCAAATAAAGCAATTAAGCGCTACAGAATGGAACCCGAAGAAATAATAGAATGCGCAAAAAGAGGAGTAGAAATGGAGCT
TAAAACGATAGTACTTCAAAGTGGTGAGGACAGGTATTATAAAATAACCACTTTGTGTAACATAGTAGAAAAAATAAAGG
AGATGGATGTAGCTGTCACACTAATCATCGGAGAACTCTCTAAAAAAGAATATGCAGAATTAAAAAAAGCAGGTGCCGAC
AGGTATCTTCTTCGCATTGAAACTACAAATAGAGAACTTTATCGGAAGCTACATCCTGGCATGAGTTATGAAAACCGCTT
AAGGTGTCTCATGGATTTAAGAGAACTGGGATATGAGGTAGGCACAGGAAGTCTGATTGGACTTCCCGGTCAAACTTTAG
AAATGCTGGCAGATGACCTCTTGTTTTTTAAAAAAATAAATGCCGACATGTTAGGTATTGGTCCTTTTATACCCTGTGAA
AACACTCCTTTAGAAAAAGAAAAAGGTGGAGATGTTAATTTAGTGCTCAAGATGATAGCTCTTTCAAGGCTTCTTCTTCC
TGATATAAATATACCTGCCACTACCGCTTTAGCAGTCAAAGACAAGGATGGCTACGCAAAAGGGCTTCAGTGCGGAGCCA
ATGTAATAATGCCAAATATAGGAATAGAAGAGTATAAAAGGCTTTACAAGCTCTATCCGGGCAAAGTGCCGGATGACCCC
AATGAAGCTGTAAACAGTATTGAAAATATCAAAAAAGTAATCCTATCACAAAATAGAAGTATTGGCAAAGATAAAGGCTA
TCGCAAAAAGGTCTTTCACTGA

Upstream 100 bases:

>100_bases
TATCAAAAATTTTGTTAAGAAAATAGTTTAACAATTCAATTGATTTTTTGTTGACAAATGTTTTATACTTATTATGAGAA
GAAAGTGAGTGTGATATGCC

Downstream 100 bases:

>100_bases
TGCGATAGCCTTTGTTTTAGGATATTGCACCTACCGGACATACATCGATGCATCTTCCACAGTCTCTACATTTATTTGGA
TCTATGTGAGCAACACCATC

Product: biotin synthase

Products: biotin

Alternate protein names: NA

Number of amino acids: Translated: 353; Mature: 353

Protein sequence:

>353_residues
MIKELTAEKNISSLIEKLEKQHSLDKKGLIMLLSMENPSELYKAADRVRKKYVGDEVHLRGLIEFSNYCHNTCFYCGLRG
PNKAIKRYRMEPEEIIECAKRGVEMELKTIVLQSGEDRYYKITTLCNIVEKIKEMDVAVTLIIGELSKKEYAELKKAGAD
RYLLRIETTNRELYRKLHPGMSYENRLRCLMDLRELGYEVGTGSLIGLPGQTLEMLADDLLFFKKINADMLGIGPFIPCE
NTPLEKEKGGDVNLVLKMIALSRLLLPDINIPATTALAVKDKDGYAKGLQCGANVIMPNIGIEEYKRLYKLYPGKVPDDP
NEAVNSIENIKKVILSQNRSIGKDKGYRKKVFH

Sequences:

>Translated_353_residues
MIKELTAEKNISSLIEKLEKQHSLDKKGLIMLLSMENPSELYKAADRVRKKYVGDEVHLRGLIEFSNYCHNTCFYCGLRG
PNKAIKRYRMEPEEIIECAKRGVEMELKTIVLQSGEDRYYKITTLCNIVEKIKEMDVAVTLIIGELSKKEYAELKKAGAD
RYLLRIETTNRELYRKLHPGMSYENRLRCLMDLRELGYEVGTGSLIGLPGQTLEMLADDLLFFKKINADMLGIGPFIPCE
NTPLEKEKGGDVNLVLKMIALSRLLLPDINIPATTALAVKDKDGYAKGLQCGANVIMPNIGIEEYKRLYKLYPGKVPDDP
NEAVNSIENIKKVILSQNRSIGKDKGYRKKVFH
>Mature_353_residues
MIKELTAEKNISSLIEKLEKQHSLDKKGLIMLLSMENPSELYKAADRVRKKYVGDEVHLRGLIEFSNYCHNTCFYCGLRG
PNKAIKRYRMEPEEIIECAKRGVEMELKTIVLQSGEDRYYKITTLCNIVEKIKEMDVAVTLIIGELSKKEYAELKKAGAD
RYLLRIETTNRELYRKLHPGMSYENRLRCLMDLRELGYEVGTGSLIGLPGQTLEMLADDLLFFKKINADMLGIGPFIPCE
NTPLEKEKGGDVNLVLKMIALSRLLLPDINIPATTALAVKDKDGYAKGLQCGANVIMPNIGIEEYKRLYKLYPGKVPDDP
NEAVNSIENIKKVILSQNRSIGKDKGYRKKVFH

Specific function: Biotin biosynthesis; last step. [C]

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786992, Length=270, Percent_Identity=22.5925925925926, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR006638
- InterPro:   IPR007197 [H]

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM [H]

EC number: 2.8.1.6

Molecular weight: Translated: 40113; Mature: 40113

Theoretical pI: Translated: 8.79; Mature: 8.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKELTAEKNISSLIEKLEKQHSLDKKGLIMLLSMENPSELYKAADRVRKKYVGDEVHLR
CCCHHHHHCCHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHHCCCHHHHH
GLIEFSNYCHNTCFYCGLRGPNKAIKRYRMEPEEIIECAKRGVEMELKTIVLQSGEDRYY
HHHHHHHHHCCCCEEECCCCHHHHHHHHCCCHHHHHHHHHCCCCEEEHHHEEECCCCCEE
KITTLCNIVEKIKEMDVAVTLIIGELSKKEYAELKKAGADRYLLRIETTNRELYRKLHPG
HHHHHHHHHHHHHHHHHHHEEHHHHHCHHHHHHHHHCCCCEEEEEEECCCHHHHHHHCCC
MSYENRLRCLMDLRELGYEVGTGSLIGLPGQTLEMLADDLLFFKKINADMLGIGPFIPCE
CCHHHHHHHHHHHHHCCCCCCCCCEECCCCHHHHHHHHHHHHHHHHCCCEECCCCCCCCC
NTPLEKEKGGDVNLVLKMIALSRLLLPDINIPATTALAVKDKDGYAKGLQCGANVIMPNI
CCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCHHHHHHCCCCEEECCC
GIEEYKRLYKLYPGKVPDDPNEAVNSIENIKKVILSQNRSIGKDKGYRKKVFH
CHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCC
>Mature Secondary Structure
MIKELTAEKNISSLIEKLEKQHSLDKKGLIMLLSMENPSELYKAADRVRKKYVGDEVHLR
CCCHHHHHCCHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHHCCCHHHHH
GLIEFSNYCHNTCFYCGLRGPNKAIKRYRMEPEEIIECAKRGVEMELKTIVLQSGEDRYY
HHHHHHHHHCCCCEEECCCCHHHHHHHHCCCHHHHHHHHHCCCCEEEHHHEEECCCCCEE
KITTLCNIVEKIKEMDVAVTLIIGELSKKEYAELKKAGADRYLLRIETTNRELYRKLHPG
HHHHHHHHHHHHHHHHHHHEEHHHHHCHHHHHHHHHCCCCEEEEEEECCCHHHHHHHCCC
MSYENRLRCLMDLRELGYEVGTGSLIGLPGQTLEMLADDLLFFKKINADMLGIGPFIPCE
CCHHHHHHHHHHHHHCCCCCCCCCEECCCCHHHHHHHHHHHHHHHHCCCEECCCCCCCCC
NTPLEKEKGGDVNLVLKMIALSRLLLPDINIPATTALAVKDKDGYAKGLQCGANVIMPNI
CCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCHHHHHHCCCCEEECCC
GIEEYKRLYKLYPGKVPDDPNEAVNSIENIKKVILSQNRSIGKDKGYRKKVFH
CHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.002 {dethiobiotin}} [C]

Substrates: dethiobiotin; sulfur

Specific reaction: dethiobiotin + sulfur = biotin

General reaction: Sulfur atom transfer [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]