BacMap

Definition	Thermoanaerobacter tengcongensis MB4, complete genome.
Accession	NC_003869
Length	2,689,445

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The map label for this gene is SelB [H]

Identifier: 20808283

GI number: 20808283

Start: 1814326

End: 1816206

Strand: Reverse

Name: SelB [H]

Synonym: TTE1871

Alternate gene names: 20808283

Gene position: 1816206-1814326 (Counterclockwise)

Preceding gene: 20808284

Following gene: 20808282

Centisome position: 67.53

GC content: 34.45

Gene sequence:

>1881_bases
GTGAAAAATATTATAATTGGCACAGCAGGACATATTGACCACGGTAAAACGACCTTGATAAAAGCTCTTACAGGGAAGGA
TACAGATAGGCTCAAAGAAGAAAAATTAAGGGGAATTACCACGGATTTGGGATTTGCTTATTTTGATTTGCCTTCTGGCA
TAAGAGCGGGTATAATTGATGTGCCAGGCCATGAAAAATTTATTAAAAACATGCTGGCAGGGGCTCACGGTATAGATATA
GTCATGCTTGTAATTGCTGCTGATGAGGGAGTAATGCCACAGACTAAAGAACACGTGGATATACTATCTTTTCTCGATAT
AAAGGCTGGCATAATTGTGCTGACAAAATGTGATTTGGTAGAAAAAGATTGGCTTTTAATAGTAGAGGAAGATGTAAGAG
AGAATTTAAAAGGTACTTTTCTAGAAAATGCGCCAATAGTTCATGTATCGTCTGTTACAGGAGAAGGATTAGATATTTTG
GTAAATACACTGGATGAATTAGCGCAAAAAGTTAAGGAAAGAAGTTCAGAAGGAATATTTCGCCTGCCTGTAGACAGAGT
TTTTTCTATTGCCGGCTTTGGGACTGTAGTCACTGGTACTTTGATATCTGGAAAGATAAAAGTCGGAGATAAAGTGATGA
TCTATCCCAAGATGATTGAGTCAAGAGTGAGAAACCTTCAGGTGCATGAAAGAAATGTTGAGTGTGCTTTTGCAGGGCAG
AGAACTGCAATAAATTTAGCAAATGTCAAGGTAGAAGAGATAGAAAGAGGAGATGTGATTGCTCCTCCTGAGGCCATTAT
TCCTTCTACTATGATAGATGTAAAGCTCTCTCTTTTAAAAGAAGCAAAAACTCTTAAAAATAGAGAAAGAATAAGATTTT
ATACAGGTGCTTCAGAAGTAATAGGAAGAGCAGTGTTGCTTGATAGGGATGAGCTACATGGTGGAGAAAGCTGTTATGCG
CAGATTTACCTGGAGGATTGTGTCTCTGTGTTGAGAAAAGATAAATTTGTCATTAGAACTTATTCCCCCATGCTTACGAT
AGGAGGAGGTATCATATTAGATCCGAATCCGGAAAAACACAAAAGATTTGATATAGAGGTCATTGAAACGCTGAGTAACA
TTGAAAAACTTGGAGATAAATTTGTGATAGAGAAATTAGTGTATGAGTCGCTATTGCCATTGTCAGAAGAAGAAATTAAG
AGAAAAGCAAATGTGGTTGATATAGGTAAATTTTCTCTTAAAAATTTGATTAAGCTAAAATTAGAAGAAAAAGATTATTT
TTATCACCGGGAAAAGTATGAAGAGATGTGTTTGAGAACAAAAGAAATCCTCGAGGATTTTCACAGGAAAAATCCTTTAA
AAGAAGGCATTTCAAAAGAAGAGCTGAAAAATAAGCTGTTTGGAGATATTAAATCTAAGCTCTGCGATTTTATCTTTGAA
TTAATGGAAAAGGAAGAGATAATAAAGATAAAAAATCAGCTAGTAGCTTTAAAAGACTTTAAAGTCGTACTTAATGAAGA
GCAAGAAAAGTTAAAAGAAAGGATAATAAAAATTTATAGAGACTCAAAATTTGAACCTCCGAAACTTTCCGAGTTAGAAG
GGTATCCCGAAATAATTCCTATGGTAGAATATTTGGTAAATATAGGAGAGTTAGTAAAACTTGATGAGGAAATTTTTCTT
TCAAAAGATAATTATGAAGAAGCCCAAAATGTGCTGATAAATTATCTTAAGGAAAATAAGGAAATCACTTTGGCAGTTTA
TAGGGATCTGTTAAATACCAGCAGAAAATATGCTATGGCAATTTTGGAATACTTTGATTCTATTAAACTGACTAAAAGAA
TAGGAGATGTAAGAGTTCTAGCAAGGGAAATAAAGAATTAA

Upstream 100 bases:

>100_bases
GTATAGTTGATGGTGTTGTTACAATAGATGTGAGGACTTTATTAGAGGGTGATGAAGAGGTAATTCTTCATGCTCTAGAA
GAAATAACGGGGGTGTGCCA

Downstream 100 bases:

>100_bases
GCCCATGGGAACACCATGGGCTTATTATCAATAGATGGCGGGAGTATGTGGGAATCGAACCCACCTCTCGCTTAAATAGC
GAGACCGTAGATTTGAAGTC

Product: selenocysteine-specific translation elongation factor

Products: NA

Alternate protein names: SelB translation factor [H]

Number of amino acids: Translated: 626; Mature: 626

Protein sequence:

>626_residues
MKNIIIGTAGHIDHGKTTLIKALTGKDTDRLKEEKLRGITTDLGFAYFDLPSGIRAGIIDVPGHEKFIKNMLAGAHGIDI
VMLVIAADEGVMPQTKEHVDILSFLDIKAGIIVLTKCDLVEKDWLLIVEEDVRENLKGTFLENAPIVHVSSVTGEGLDIL
VNTLDELAQKVKERSSEGIFRLPVDRVFSIAGFGTVVTGTLISGKIKVGDKVMIYPKMIESRVRNLQVHERNVECAFAGQ
RTAINLANVKVEEIERGDVIAPPEAIIPSTMIDVKLSLLKEAKTLKNRERIRFYTGASEVIGRAVLLDRDELHGGESCYA
QIYLEDCVSVLRKDKFVIRTYSPMLTIGGGIILDPNPEKHKRFDIEVIETLSNIEKLGDKFVIEKLVYESLLPLSEEEIK
RKANVVDIGKFSLKNLIKLKLEEKDYFYHREKYEEMCLRTKEILEDFHRKNPLKEGISKEELKNKLFGDIKSKLCDFIFE
LMEKEEIIKIKNQLVALKDFKVVLNEEQEKLKERIIKIYRDSKFEPPKLSELEGYPEIIPMVEYLVNIGELVKLDEEIFL
SKDNYEEAQNVLINYLKENKEITLAVYRDLLNTSRKYAMAILEYFDSIKLTKRIGDVRVLAREIKN

Sequences:

>Translated_626_residues
MKNIIIGTAGHIDHGKTTLIKALTGKDTDRLKEEKLRGITTDLGFAYFDLPSGIRAGIIDVPGHEKFIKNMLAGAHGIDI
VMLVIAADEGVMPQTKEHVDILSFLDIKAGIIVLTKCDLVEKDWLLIVEEDVRENLKGTFLENAPIVHVSSVTGEGLDIL
VNTLDELAQKVKERSSEGIFRLPVDRVFSIAGFGTVVTGTLISGKIKVGDKVMIYPKMIESRVRNLQVHERNVECAFAGQ
RTAINLANVKVEEIERGDVIAPPEAIIPSTMIDVKLSLLKEAKTLKNRERIRFYTGASEVIGRAVLLDRDELHGGESCYA
QIYLEDCVSVLRKDKFVIRTYSPMLTIGGGIILDPNPEKHKRFDIEVIETLSNIEKLGDKFVIEKLVYESLLPLSEEEIK
RKANVVDIGKFSLKNLIKLKLEEKDYFYHREKYEEMCLRTKEILEDFHRKNPLKEGISKEELKNKLFGDIKSKLCDFIFE
LMEKEEIIKIKNQLVALKDFKVVLNEEQEKLKERIIKIYRDSKFEPPKLSELEGYPEIIPMVEYLVNIGELVKLDEEIFL
SKDNYEEAQNVLINYLKENKEITLAVYRDLLNTSRKYAMAILEYFDSIKLTKRIGDVRVLAREIKN
>Mature_626_residues
MKNIIIGTAGHIDHGKTTLIKALTGKDTDRLKEEKLRGITTDLGFAYFDLPSGIRAGIIDVPGHEKFIKNMLAGAHGIDI
VMLVIAADEGVMPQTKEHVDILSFLDIKAGIIVLTKCDLVEKDWLLIVEEDVRENLKGTFLENAPIVHVSSVTGEGLDIL
VNTLDELAQKVKERSSEGIFRLPVDRVFSIAGFGTVVTGTLISGKIKVGDKVMIYPKMIESRVRNLQVHERNVECAFAGQ
RTAINLANVKVEEIERGDVIAPPEAIIPSTMIDVKLSLLKEAKTLKNRERIRFYTGASEVIGRAVLLDRDELHGGESCYA
QIYLEDCVSVLRKDKFVIRTYSPMLTIGGGIILDPNPEKHKRFDIEVIETLSNIEKLGDKFVIEKLVYESLLPLSEEEIK
RKANVVDIGKFSLKNLIKLKLEEKDYFYHREKYEEMCLRTKEILEDFHRKNPLKEGISKEELKNKLFGDIKSKLCDFIFE
LMEKEEIIKIKNQLVALKDFKVVLNEEQEKLKERIIKIYRDSKFEPPKLSELEGYPEIIPMVEYLVNIGELVKLDEEIFL
SKDNYEEAQNVLINYLKENKEITLAVYRDLLNTSRKYAMAILEYFDSIKLTKRIGDVRVLAREIKN

Specific function: Translation factor necessary for the incorporation of selenocysteine into proteins. It probably replaces EF-Tu for the insertion of selenocysteine directed by the UGA codon. SelB binds GTP and GDP [H]

COG id: COG3276

COG function: function code J; Selenocysteine-specific translation elongation factor

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. SelB subfamily [H]

Homologues:

Organism=Homo sapiens, GI34147630, Length=321, Percent_Identity=30.2180685358255, Blast_Score=132, Evalue=7e-31,
Organism=Homo sapiens, GI54607086, Length=350, Percent_Identity=28, Blast_Score=130, Evalue=4e-30,
Organism=Homo sapiens, GI5729864, Length=314, Percent_Identity=26.4331210191083, Blast_Score=97, Evalue=5e-20,
Organism=Homo sapiens, GI223555963, Length=314, Percent_Identity=26.4331210191083, Blast_Score=96, Evalue=8e-20,
Organism=Homo sapiens, GI4503507, Length=264, Percent_Identity=28.4090909090909, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI82546879, Length=379, Percent_Identity=24.2744063324538, Blast_Score=86, Evalue=1e-16,
Organism=Homo sapiens, GI53729339, Length=220, Percent_Identity=30, Blast_Score=83, Evalue=7e-16,
Organism=Homo sapiens, GI53729337, Length=220, Percent_Identity=30, Blast_Score=83, Evalue=7e-16,
Organism=Homo sapiens, GI45593140, Length=358, Percent_Identity=24.3016759776536, Blast_Score=83, Evalue=9e-16,
Organism=Homo sapiens, GI194097354, Length=314, Percent_Identity=27.0700636942675, Blast_Score=77, Evalue=4e-14,
Organism=Homo sapiens, GI194018520, Length=314, Percent_Identity=27.0700636942675, Blast_Score=77, Evalue=4e-14,
Organism=Homo sapiens, GI194018522, Length=315, Percent_Identity=27.3015873015873, Blast_Score=77, Evalue=5e-14,
Organism=Escherichia coli, GI2367247, Length=633, Percent_Identity=31.1216429699842, Blast_Score=277, Evalue=1e-75,
Organism=Escherichia coli, GI1790412, Length=331, Percent_Identity=32.9305135951662, Blast_Score=139, Evalue=4e-34,
Organism=Escherichia coli, GI1789737, Length=331, Percent_Identity=32.9305135951662, Blast_Score=139, Evalue=4e-34,
Organism=Escherichia coli, GI1789108, Length=268, Percent_Identity=30.9701492537313, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI1789559, Length=206, Percent_Identity=29.6116504854369, Blast_Score=70, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI25141371, Length=380, Percent_Identity=33.4210526315789, Blast_Score=167, Evalue=1e-41,
Organism=Caenorhabditis elegans, GI17506081, Length=284, Percent_Identity=32.7464788732394, Blast_Score=120, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17556456, Length=392, Percent_Identity=29.8469387755102, Blast_Score=116, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI17552884, Length=348, Percent_Identity=27.0114942528736, Blast_Score=101, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17569207, Length=348, Percent_Identity=27.0114942528736, Blast_Score=101, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI115532067, Length=328, Percent_Identity=28.3536585365854, Blast_Score=99, Evalue=8e-21,
Organism=Caenorhabditis elegans, GI115532065, Length=328, Percent_Identity=28.0487804878049, Blast_Score=98, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17509919, Length=278, Percent_Identity=27.6978417266187, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI71994658, Length=95, Percent_Identity=40, Blast_Score=75, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI32566629, Length=317, Percent_Identity=25.2365930599369, Blast_Score=70, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6324761, Length=309, Percent_Identity=30.7443365695793, Blast_Score=131, Evalue=3e-31,
Organism=Saccharomyces cerevisiae, GI6325337, Length=325, Percent_Identity=27.6923076923077, Blast_Score=106, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6319594, Length=325, Percent_Identity=27.6923076923077, Blast_Score=106, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6320863, Length=262, Percent_Identity=29.0076335877863, Blast_Score=97, Evalue=6e-21,
Organism=Saccharomyces cerevisiae, GI6322937, Length=327, Percent_Identity=26.9113149847095, Blast_Score=76, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6324550, Length=171, Percent_Identity=30.4093567251462, Blast_Score=64, Evalue=5e-11,
Organism=Drosophila melanogaster, GI19921738, Length=278, Percent_Identity=31.294964028777, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI281363316, Length=384, Percent_Identity=28.125, Blast_Score=128, Evalue=9e-30,
Organism=Drosophila melanogaster, GI17864358, Length=384, Percent_Identity=28.125, Blast_Score=128, Evalue=9e-30,
Organism=Drosophila melanogaster, GI19922690, Length=327, Percent_Identity=31.1926605504587, Blast_Score=126, Evalue=5e-29,
Organism=Drosophila melanogaster, GI45550900, Length=313, Percent_Identity=26.1980830670927, Blast_Score=98, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24647046, Length=266, Percent_Identity=28.9473684210526, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24647048, Length=266, Percent_Identity=28.9473684210526, Blast_Score=96, Evalue=6e-20,
Organism=Drosophila melanogaster, GI19922512, Length=386, Percent_Identity=24.0932642487047, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24654852, Length=386, Percent_Identity=24.0932642487047, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI28572034, Length=169, Percent_Identity=31.9526627218935, Blast_Score=79, Evalue=1e-14,
Organism=Drosophila melanogaster, GI17137380, Length=308, Percent_Identity=25.6493506493506, Blast_Score=72, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015189
- InterPro:   IPR015190
- InterPro:   IPR015191
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR009000
- InterPro:   IPR004535
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF09105 SelB-wing_1; PF09106 SelB-wing_2; PF09107 SelB-wing_3 [H]

EC number: NA

Molecular weight: Translated: 71352; Mature: 71352

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNIIIGTAGHIDHGKTTLIKALTGKDTDRLKEEKLRGITTDLGFAYFDLPSGIRAGIID
CCCEEEECCCCCCCCHHHHHHHHCCCCHHHHHHHHHHCCHHHCCEEEEECCCCCCCCEEE
VPGHEKFIKNMLAGAHGIDIVMLVIAADEGVMPQTKEHVDILSFLDIKAGIIVLTKCDLV
CCCHHHHHHHHHCCCCCHHEEEEEEECCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCC
EKDWLLIVEEDVRENLKGTFLENAPIVHVSSVTGEGLDILVNTLDELAQKVKERSSEGIF
CCCCEEEEEHHHHCCCCCCEECCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCEE
RLPVDRVFSIAGFGTVVTGTLISGKIKVGDKVMIYPKMIESRVRNLQVHERNVECAFAGQ
ECCHHHHHHHHCCHHHHHHHEEECEEEECCEEEEEHHHHHHHHHCCEEECCCCEEEECCC
RTAINLANVKVEEIERGDVIAPPEAIIPSTMIDVKLSLLKEAKTLKNRERIRFYTGASEV
CCEEEEECCCHHHHCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHCCCCCEEEEECHHHH
IGRAVLLDRDELHGGESCYAQIYLEDCVSVLRKDKFVIRTYSPMLTIGGGIILDPNPEKH
HHHHHEEECHHCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCEEECCEEEECCCCCHH
KRFDIEVIETLSNIEKLGDKFVIEKLVYESLLPLSEEEIKRKANVVDIGKFSLKNLIKLK
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCEEECCHHHHHHHHHEE
LEEKDYFYHREKYEEMCLRTKEILEDFHRKNPLKEGISKEELKNKLFGDIKSKLCDFIFE
ECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHH
LMEKEEIIKIKNQLVALKDFKVVLNEEQEKLKERIIKIYRDSKFEPPKLSELEGYPEIIP
HHCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCHHCCCCHHHHH
MVEYLVNIGELVKLDEEIFLSKDNYEEAQNVLINYLKENKEITLAVYRDLLNTSRKYAMA
HHHHHHCHHHHHHCCHHHEECCCCHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHH
ILEYFDSIKLTKRIGDVRVLAREIKN
HHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKNIIIGTAGHIDHGKTTLIKALTGKDTDRLKEEKLRGITTDLGFAYFDLPSGIRAGIID
CCCEEEECCCCCCCCHHHHHHHHCCCCHHHHHHHHHHCCHHHCCEEEEECCCCCCCCEEE
VPGHEKFIKNMLAGAHGIDIVMLVIAADEGVMPQTKEHVDILSFLDIKAGIIVLTKCDLV
CCCHHHHHHHHHCCCCCHHEEEEEEECCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCC
EKDWLLIVEEDVRENLKGTFLENAPIVHVSSVTGEGLDILVNTLDELAQKVKERSSEGIF
CCCCEEEEEHHHHCCCCCCEECCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCEE
RLPVDRVFSIAGFGTVVTGTLISGKIKVGDKVMIYPKMIESRVRNLQVHERNVECAFAGQ
ECCHHHHHHHHCCHHHHHHHEEECEEEECCEEEEEHHHHHHHHHCCEEECCCCEEEECCC
RTAINLANVKVEEIERGDVIAPPEAIIPSTMIDVKLSLLKEAKTLKNRERIRFYTGASEV
CCEEEEECCCHHHHCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHCCCCCEEEEECHHHH
IGRAVLLDRDELHGGESCYAQIYLEDCVSVLRKDKFVIRTYSPMLTIGGGIILDPNPEKH
HHHHHEEECHHCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCEEECCEEEECCCCCHH
KRFDIEVIETLSNIEKLGDKFVIEKLVYESLLPLSEEEIKRKANVVDIGKFSLKNLIKLK
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCEEECCHHHHHHHHHEE
LEEKDYFYHREKYEEMCLRTKEILEDFHRKNPLKEGISKEELKNKLFGDIKSKLCDFIFE
ECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHH
LMEKEEIIKIKNQLVALKDFKVVLNEEQEKLKERIIKIYRDSKFEPPKLSELEGYPEIIP
HHCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCHHCCCCHHHHH
MVEYLVNIGELVKLDEEIFLSKDNYEEAQNVLINYLKENKEITLAVYRDLLNTSRKYAMA
HHHHHHCHHHHHHCCHHHEECCCCHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHH
ILEYFDSIKLTKRIGDVRVLAREIKN
HHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8893853 [H]