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The map label for this gene is AmyA2 [H]

Identifier: 20808248

GI number: 20808248

Start: 1781977

End: 1783551

Strand: Reverse

Name: AmyA2 [H]

Synonym: TTE1832

Alternate gene names: 20808248

Gene position: 1783551-1781977 (Counterclockwise)

Preceding gene: 20808249

Following gene: 20808247

Centisome position: 66.32

GC content: 39.62

Gene sequence:

>1575_bases
ATGAGAAAAAATTTAAAAGCATTTGTGGCGCTTTTTGCAGCAATTTTGCTGTTCTTTAGTGGGTGCAGTAGCAAGCAAGA
GGCAAAAGCCCCAAAGTCAGAAGTCATATATCAGGTTATGGTGGATAGGTTTTACAACGGAGACCCTTCAAATGACGACC
CAGAAGTGAGCAAAGGAATGTTTGACCCCACCCATACCAACTGGAGGATGTACTGGGGAGGGGATTTGAAAGGACTGACA
GAAAAAATCCCTTATATAAAGGGGATGGGGGTCACTGCTATCTGGATTTCTCCAGTAGTGGATAATATAAATAAACCAGC
CGTATATAATGGCGAGATTAATGCCCCCTATCACGGATACTGGGCAAGAGATTTCAAAAGAGTAGAAGAACACTTTGGTA
CATGGGAGGACTTTGACAATTTTGTAAAGGTTGCGCATGAGAATGGAATAAAAGTGATTTTAGATTTTGCGCCAAATCAC
ACGAGCCCTGCCGATGAAGAAAATCCTGATTTTGCAGAAAATGGCGCATTATATGACGATGGGAAGCTATTAGGTACTTA
CAGTAATGATTCACTTAAGCTTTTTCACCACAATGGCAGCATAAGCAACTGGAATAATTTAAAGGAACTTCAGGATAAAA
ATTTGTTTGATTTGGCAGATTTGGACCAGAGCAACCCTATTGTTGATAAATACTTAAAAGACTCTATTAAGTTATGGTTT
AATCATGAAATTGATGGAGTAAGGCTGGATGCTGCAAAGCATATGCCTATGGAGTGGGTGAAAAGCTTTGCTAACACTAT
TTACAGTATTAAAAAGGATGTGCTTCTTTTTGGTGAATGGATGTTAAGCGGTCCTACTGACCCGTTGTATGGGTATAATA
TACAGTTTGCTAATACTACCGGCTTTTCTGTCCTGGATTTTATGTTAAACGGTGCTATAAGAGATGTATTTGGCAAGGGG
TACGGATTTGAGAGGTTAAATGACACGTTAGAGGATACCAATAAAGATTACGAAAATCCTTATAAGCTGGTTGCTTTTAT
AGACAACCATGACATGCCTAGATTTCTGTCCTTGAACAATGACAAGGATAAATTACACGAAGCTATTGCTTTTGTAATGA
CGACTCGAGGGATACCTGTAATATACTATGGCACTGAGCAGTACCTTCATAACGACACAAATGGCGGTAATGACCCGTAT
AATAGACCAATGATGGAAAAGTTCGATGAGAGCACAAAGGCTTATACATTGATAAAAGAGCTGTCAAGGTTAAGGCAGCT
CACTCCAGCCCTACAGTATGGAACTACTACGGCAAGGTATATTTCTGACGATGTGTATATTTATGAAAGGCAGTATGGAA
AAGATGTGGTGCTGGTTGCGATAAATAAAGGAGAGAAAACTACTGTTAAGGCAGTAAAAACATCTTTGAGGAAGGGAATT
TATAAAGACTACCTTAAAGGTCTGTTGGAGGGTGTTGAGTTAAAAGTCACAAAAGGCAATGGAGAAAACCTAGTGCAAGG
TTTGACTTTACCTGGGGACAGCGTAAGTGTGTGGACAAATGTGAAAGCGAAGTAA

Upstream 100 bases:

>100_bases
AGCGATTGCAATAAAATTTTCTACTTTGCTCTTTTTTAATTGCATTTCAGGAAAGCGATTGCTGTAATATTATGGTATTA
AAAGTGTAAGGGGGAAAGAG

Downstream 100 bases:

>100_bases
AATTTTGGGGTGCACCCCAAATTATTATAAAACCAATTTAAGAGGAGGATTTGTATGAAGAAGTATTCAAAAATACTTGC
GTTGTTGACAGCAATGGTGT

Product: glycosidases

Products: NA

Alternate protein names: Glucan 1,4-alpha-maltohydrolase [H]

Number of amino acids: Translated: 524; Mature: 524

Protein sequence:

>524_residues
MRKNLKAFVALFAAILLFFSGCSSKQEAKAPKSEVIYQVMVDRFYNGDPSNDDPEVSKGMFDPTHTNWRMYWGGDLKGLT
EKIPYIKGMGVTAIWISPVVDNINKPAVYNGEINAPYHGYWARDFKRVEEHFGTWEDFDNFVKVAHENGIKVILDFAPNH
TSPADEENPDFAENGALYDDGKLLGTYSNDSLKLFHHNGSISNWNNLKELQDKNLFDLADLDQSNPIVDKYLKDSIKLWF
NHEIDGVRLDAAKHMPMEWVKSFANTIYSIKKDVLLFGEWMLSGPTDPLYGYNIQFANTTGFSVLDFMLNGAIRDVFGKG
YGFERLNDTLEDTNKDYENPYKLVAFIDNHDMPRFLSLNNDKDKLHEAIAFVMTTRGIPVIYYGTEQYLHNDTNGGNDPY
NRPMMEKFDESTKAYTLIKELSRLRQLTPALQYGTTTARYISDDVYIYERQYGKDVVLVAINKGEKTTVKAVKTSLRKGI
YKDYLKGLLEGVELKVTKGNGENLVQGLTLPGDSVSVWTNVKAK

Sequences:

>Translated_524_residues
MRKNLKAFVALFAAILLFFSGCSSKQEAKAPKSEVIYQVMVDRFYNGDPSNDDPEVSKGMFDPTHTNWRMYWGGDLKGLT
EKIPYIKGMGVTAIWISPVVDNINKPAVYNGEINAPYHGYWARDFKRVEEHFGTWEDFDNFVKVAHENGIKVILDFAPNH
TSPADEENPDFAENGALYDDGKLLGTYSNDSLKLFHHNGSISNWNNLKELQDKNLFDLADLDQSNPIVDKYLKDSIKLWF
NHEIDGVRLDAAKHMPMEWVKSFANTIYSIKKDVLLFGEWMLSGPTDPLYGYNIQFANTTGFSVLDFMLNGAIRDVFGKG
YGFERLNDTLEDTNKDYENPYKLVAFIDNHDMPRFLSLNNDKDKLHEAIAFVMTTRGIPVIYYGTEQYLHNDTNGGNDPY
NRPMMEKFDESTKAYTLIKELSRLRQLTPALQYGTTTARYISDDVYIYERQYGKDVVLVAINKGEKTTVKAVKTSLRKGI
YKDYLKGLLEGVELKVTKGNGENLVQGLTLPGDSVSVWTNVKAK
>Mature_524_residues
MRKNLKAFVALFAAILLFFSGCSSKQEAKAPKSEVIYQVMVDRFYNGDPSNDDPEVSKGMFDPTHTNWRMYWGGDLKGLT
EKIPYIKGMGVTAIWISPVVDNINKPAVYNGEINAPYHGYWARDFKRVEEHFGTWEDFDNFVKVAHENGIKVILDFAPNH
TSPADEENPDFAENGALYDDGKLLGTYSNDSLKLFHHNGSISNWNNLKELQDKNLFDLADLDQSNPIVDKYLKDSIKLWF
NHEIDGVRLDAAKHMPMEWVKSFANTIYSIKKDVLLFGEWMLSGPTDPLYGYNIQFANTTGFSVLDFMLNGAIRDVFGKG
YGFERLNDTLEDTNKDYENPYKLVAFIDNHDMPRFLSLNNDKDKLHEAIAFVMTTRGIPVIYYGTEQYLHNDTNGGNDPY
NRPMMEKFDESTKAYTLIKELSRLRQLTPALQYGTTTARYISDDVYIYERQYGKDVVLVAINKGEKTTVKAVKTSLRKGI
YKDYLKGLLEGVELKVTKGNGENLVQGLTLPGDSVSVWTNVKAK

Specific function: Converts starch into maltose [H]

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 IPT/TIG domain [H]

Homologues:

Organism=Homo sapiens, GI187423904, Length=191, Percent_Identity=29.3193717277487, Blast_Score=83, Evalue=8e-16,
Organism=Escherichia coli, GI1786604, Length=540, Percent_Identity=24.2592592592593, Blast_Score=108, Evalue=6e-25,
Organism=Escherichia coli, GI1789995, Length=150, Percent_Identity=35.3333333333333, Blast_Score=88, Evalue=2e-18,
Organism=Escherichia coli, GI1790687, Length=187, Percent_Identity=31.0160427807487, Blast_Score=75, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24586587, Length=198, Percent_Identity=33.3333333333333, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24583745, Length=193, Percent_Identity=34.1968911917098, Blast_Score=92, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24586599, Length=189, Percent_Identity=32.2751322751323, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24586591, Length=197, Percent_Identity=33.502538071066, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24586589, Length=197, Percent_Identity=31.9796954314721, Blast_Score=90, Evalue=4e-18,
Organism=Drosophila melanogaster, GI45549022, Length=193, Percent_Identity=32.1243523316062, Blast_Score=90, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24583747, Length=192, Percent_Identity=31.25, Blast_Score=89, Evalue=5e-18,
Organism=Drosophila melanogaster, GI24583749, Length=192, Percent_Identity=31.25, Blast_Score=89, Evalue=6e-18,
Organism=Drosophila melanogaster, GI24586597, Length=194, Percent_Identity=32.4742268041237, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24586593, Length=193, Percent_Identity=28.4974093264249, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI221330053, Length=194, Percent_Identity=32.4742268041237, Blast_Score=80, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006048
- InterPro:   IPR013784
- InterPro:   IPR006046
- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR006589
- InterPro:   IPR002044
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR013783
- InterPro:   IPR014756
- InterPro:   IPR002909 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02806 Alpha-amylase_C; PF00686 CBM_20; PF01833 TIG [H]

EC number: =3.2.1.133 [H]

Molecular weight: Translated: 59641; Mature: 59641

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKNLKAFVALFAAILLFFSGCSSKQEAKAPKSEVIYQVMVDRFYNGDPSNDDPEVSKGM
CCCHHHHHHHHHHHHHHHHCCCCCHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCHHHCCC
FDPTHTNWRMYWGGDLKGLTEKIPYIKGMGVTAIWISPVVDNINKPAVYNGEINAPYHGY
CCCCCCCEEEEECCCCCHHHHHCCCCCCCCEEEEEEEHHHCCCCCCEEECCCCCCCCCCH
WARDFKRVEEHFGTWEDFDNFVKVAHENGIKVILDFAPNHTSPADEENPDFAENGALYDD
HHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCCEEEC
GKLLGTYSNDSLKLFHHNGSISNWNNLKELQDKNLFDLADLDQSNPIVDKYLKDSIKLWF
CEEEEEECCCCEEEEEECCCCCCCHHHHHHHCCCCCCHHCCCCCCCHHHHHHHHHHEEEE
NHEIDGVRLDAAKHMPMEWVKSFANTIYSIKKDVLLFGEWMLSGPTDPLYGYNIQFANTT
CCCCCCEEECCHHCCCHHHHHHHHHHHHHHHHHHHEEEHHHCCCCCCCCCCEEEEEECCC
GFSVLDFMLNGAIRDVFGKGYGFERLNDTLEDTNKDYENPYKLVAFIDNHDMPRFLSLNN
CHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCEEECCC
DKDKLHEAIAFVMTTRGIPVIYYGTEQYLHNDTNGGNDPYNRPMMEKFDESTKAYTLIKE
CHHHHHHHHHHHHHCCCCEEEEECCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
LSRLRQLTPALQYGTTTARYISDDVYIYERQYGKDVVLVAINKGEKTTVKAVKTSLRKGI
HHHHHHHHHHHHCCCCHHHEECCCEEEEEECCCCEEEEEEECCCCCHHHHHHHHHHHHHH
YKDYLKGLLEGVELKVTKGNGENLVQGLTLPGDSVSVWTNVKAK
HHHHHHHHHCCCEEEEECCCCCHHHEEECCCCCCEEEEECCCCC
>Mature Secondary Structure
MRKNLKAFVALFAAILLFFSGCSSKQEAKAPKSEVIYQVMVDRFYNGDPSNDDPEVSKGM
CCCHHHHHHHHHHHHHHHHCCCCCHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCHHHCCC
FDPTHTNWRMYWGGDLKGLTEKIPYIKGMGVTAIWISPVVDNINKPAVYNGEINAPYHGY
CCCCCCCEEEEECCCCCHHHHHCCCCCCCCEEEEEEEHHHCCCCCCEEECCCCCCCCCCH
WARDFKRVEEHFGTWEDFDNFVKVAHENGIKVILDFAPNHTSPADEENPDFAENGALYDD
HHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCCEEEC
GKLLGTYSNDSLKLFHHNGSISNWNNLKELQDKNLFDLADLDQSNPIVDKYLKDSIKLWF
CEEEEEECCCCEEEEEECCCCCCCHHHHHHHCCCCCCHHCCCCCCCHHHHHHHHHHEEEE
NHEIDGVRLDAAKHMPMEWVKSFANTIYSIKKDVLLFGEWMLSGPTDPLYGYNIQFANTT
CCCCCCEEECCHHCCCHHHHHHHHHHHHHHHHHHHEEEHHHCCCCCCCCCCEEEEEECCC
GFSVLDFMLNGAIRDVFGKGYGFERLNDTLEDTNKDYENPYKLVAFIDNHDMPRFLSLNN
CHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCEEECCC
DKDKLHEAIAFVMTTRGIPVIYYGTEQYLHNDTNGGNDPYNRPMMEKFDESTKAYTLIKE
CHHHHHHHHHHHHHCCCCEEEEECCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
LSRLRQLTPALQYGTTTARYISDDVYIYERQYGKDVVLVAINKGEKTTVKAVKTSLRKGI
HHHHHHHHHHHHCCCCHHHEECCCEEEEEECCCCEEEEEEECCCCCHHHHHHHHHHHHHH
YKDYLKGLLEGVELKVTKGNGENLVQGLTLPGDSVSVWTNVKAK
HHHHHHHHHCCCEEEEECCCCCHHHEEECCCCCCEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10387084 [H]