Click here to switch to the map view.

The map label for this gene is MalE [H]

Identifier: 20808247

GI number: 20808247

Start: 1780672

End: 1781922

Strand: Reverse

Name: MalE [H]

Synonym: TTE1831

Alternate gene names: 20808247

Gene position: 1781922-1780672 (Counterclockwise)

Preceding gene: 20808248

Following gene: 20808246

Centisome position: 66.26

GC content: 42.37

Gene sequence:

>1251_bases
ATGAAGAAGTATTCAAAAATACTTGCGTTGTTGACAGCAATGGTGTTTGTGCTGTCAATTGCTCTGACTGGCTGTGGCGG
CGGCAGCAAGACAAGTGAAAGTCAGTCATCACAGACTCAGACAGCAGAGCAGAAAAAGGAACCTGTAGAATTGATAGTAT
GGTCCCACTTGACAGACCCTGAAATAGCAAAAGTTCAAGAAATTGCTAACAAATGGGCTGAACAGACAGGAAACAAGGTC
AAAGTTCTAGCAGACCAGAGCGATTTCCAAGCTTTTGCTACAGCTGCTCAAAGCGGTAAAGGGCCAGACATAATGTTTGG
ATTGCCCCATGACAACTTGGGAACTTTCCAAAAGGCAGGACTTTTAGCTGAGGTTCCAGAGGGAGTTATAAACAAAGATG
ACTATGTGCCAATGAGCATAAGCGCTGTTTCCTATGATGGAAAGATGTATGCAGTGCCAATTGCTATGGAAACTTATGCC
CTTTTCTACAATACGGATAAAGTCAAAACTCCTCCAGCTACATTAGATGACTTGATTAAATTGGGGAAAGAAGTAGGGTT
CCAGTATGACGTCAATAACTTCTATTTCAGCTTTGCTTTTATCGCAGCGTATGGCGGATATGTTTTTAAAGACACAGGAG
GCGGACTTGATCCAAACGACATAGGGCTCAACAACGAAGGCGCAAAGAAAGGGTTAGAGCTTATAAAAGATTTTGTTCAG
AAATATAAATTCATGCCTCCAGATATAAACTATGACATGGCAAAAGGTAACTTCCAGAGTGGCAAAATTGGACTTTATAT
AAGCGGTCCATGGGATGTGGACGGATTCAAGAAAGCCAATGTTCCATTTAAAGTTGCACCACTTCCCAAAATTGATGGCA
AGCCAACTCCTTCTTTTGCAGGTGTGCAGGCTGCATTTGTGAGCGCAAATTCAAAGCATCAGAAAGAAGCTTGGGATTTG
ATGAAGTACTTGGTTGAAAATACAGGGCTTCCATTGTTTGAGACAGGAAACAGAATTCCTGTGATTAAGTCCTTGCTGGA
CAATCCAGAAGTTAAGGGCAACGAGATACTAAGTGCATTTGCTGAACAGGCACAGCATGCTATTCCAATGCCAAATATTC
CTCAGATGAGCCAGGTATGGACACCTGCTGGTAATGCGCTGCAGCTTATAACCTCAGGAAAAGTGCCTGTTGACAAAGCA
GCTGACGATATGGTAAAACAAATTAAACAGGGAATTGCTACAATGCAGTAA

Upstream 100 bases:

>100_bases
ACCTGGGGACAGCGTAAGTGTGTGGACAAATGTGAAAGCGAAGTAAAATTTTGGGGTGCACCCCAAATTATTATAAAACC
AATTTAAGAGGAGGATTTGT

Downstream 100 bases:

>100_bases
AAAGGGGCTTTATGCCCCTTTTTTTAAGGGAAAGGAGGTACAGGATGAACCAGCATAAATCGTTGAAAGATAAGCTAAGA
CCTTATGCCTATTTGTCTCC

Product: maltose-binding periplasmic protein

Products: ADP; phosphate; maltose [Cytoplasm] [C]

Alternate protein names: MMBP; Maltodextrin-binding protein [H]

Number of amino acids: Translated: 416; Mature: 416

Protein sequence:

>416_residues
MKKYSKILALLTAMVFVLSIALTGCGGGSKTSESQSSQTQTAEQKKEPVELIVWSHLTDPEIAKVQEIANKWAEQTGNKV
KVLADQSDFQAFATAAQSGKGPDIMFGLPHDNLGTFQKAGLLAEVPEGVINKDDYVPMSISAVSYDGKMYAVPIAMETYA
LFYNTDKVKTPPATLDDLIKLGKEVGFQYDVNNFYFSFAFIAAYGGYVFKDTGGGLDPNDIGLNNEGAKKGLELIKDFVQ
KYKFMPPDINYDMAKGNFQSGKIGLYISGPWDVDGFKKANVPFKVAPLPKIDGKPTPSFAGVQAAFVSANSKHQKEAWDL
MKYLVENTGLPLFETGNRIPVIKSLLDNPEVKGNEILSAFAEQAQHAIPMPNIPQMSQVWTPAGNALQLITSGKVPVDKA
ADDMVKQIKQGIATMQ

Sequences:

>Translated_416_residues
MKKYSKILALLTAMVFVLSIALTGCGGGSKTSESQSSQTQTAEQKKEPVELIVWSHLTDPEIAKVQEIANKWAEQTGNKV
KVLADQSDFQAFATAAQSGKGPDIMFGLPHDNLGTFQKAGLLAEVPEGVINKDDYVPMSISAVSYDGKMYAVPIAMETYA
LFYNTDKVKTPPATLDDLIKLGKEVGFQYDVNNFYFSFAFIAAYGGYVFKDTGGGLDPNDIGLNNEGAKKGLELIKDFVQ
KYKFMPPDINYDMAKGNFQSGKIGLYISGPWDVDGFKKANVPFKVAPLPKIDGKPTPSFAGVQAAFVSANSKHQKEAWDL
MKYLVENTGLPLFETGNRIPVIKSLLDNPEVKGNEILSAFAEQAQHAIPMPNIPQMSQVWTPAGNALQLITSGKVPVDKA
ADDMVKQIKQGIATMQ
>Mature_416_residues
MKKYSKILALLTAMVFVLSIALTGCGGGSKTSESQSSQTQTAEQKKEPVELIVWSHLTDPEIAKVQEIANKWAEQTGNKV
KVLADQSDFQAFATAAQSGKGPDIMFGLPHDNLGTFQKAGLLAEVPEGVINKDDYVPMSISAVSYDGKMYAVPIAMETYA
LFYNTDKVKTPPATLDDLIKLGKEVGFQYDVNNFYFSFAFIAAYGGYVFKDTGGGLDPNDIGLNNEGAKKGLELIKDFVQ
KYKFMPPDINYDMAKGNFQSGKIGLYISGPWDVDGFKKANVPFKVAPLPKIDGKPTPSFAGVQAAFVSANSKHQKEAWDL
MKYLVENTGLPLFETGNRIPVIKSLLDNPEVKGNEILSAFAEQAQHAIPMPNIPQMSQVWTPAGNALQLITSGKVPVDKA
ADDMVKQIKQGIATMQ

Specific function: Involved in the high-affinity maltose membrane transport system malEFGK. Initial receptor for the active transport of and chemotaxis toward maltooligosaccharides [H]

COG id: COG2182

COG function: function code G; Maltose-binding periplasmic proteins/domains

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 1 family [H]

Homologues:

Organism=Escherichia coli, GI1790466, Length=379, Percent_Identity=32.4538258575198, Blast_Score=188, Evalue=5e-49,

Paralogues:

None

Copy number: 660 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 720 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 240 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006060
- InterPro:   IPR006059
- InterPro:   IPR006061 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 45241; Mature: 45241

Theoretical pI: Translated: 5.81; Mature: 5.81

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKYSKILALLTAMVFVLSIALTGCGGGSKTSESQSSQTQTAEQKKEPVELIVWSHLTDP
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCH
EIAKVQEIANKWAEQTGNKVKVLADQSDFQAFATAAQSGKGPDIMFGLPHDNLGTFQKAG
HHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHCC
LLAEVPEGVINKDDYVPMSISAVSYDGKMYAVPIAMETYALFYNTDKVKTPPATLDDLIK
CHHHCCCCCCCCCCCCCEEEEEEEECCEEEEEEEEEEEEEEEEECCCCCCCCCCHHHHHH
LGKEVGFQYDVNNFYFSFAFIAAYGGYVFKDTGGGLDPNDIGLNNEGAKKGLELIKDFVQ
HHHHCCCEEECCHHHHHHHHHHHHCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
KYKFMPPDINYDMAKGNFQSGKIGLYISGPWDVDGFKKANVPFKVAPLPKIDGKPTPSFA
HHCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCEECCCCEEECCCCCCCCCCCCCCC
GVQAAFVSANSKHQKEAWDLMKYLVENTGLPLFETGNRIPVIKSLLDNPEVKGNEILSAF
CCEEEEECCCCCHHHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHCCCCCCHHHHHHHH
AEQAQHAIPMPNIPQMSQVWTPAGNALQLITSGKVPVDKAADDMVKQIKQGIATMQ
HHHHHHCCCCCCCCCHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKKYSKILALLTAMVFVLSIALTGCGGGSKTSESQSSQTQTAEQKKEPVELIVWSHLTDP
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCH
EIAKVQEIANKWAEQTGNKVKVLADQSDFQAFATAAQSGKGPDIMFGLPHDNLGTFQKAG
HHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHCC
LLAEVPEGVINKDDYVPMSISAVSYDGKMYAVPIAMETYALFYNTDKVKTPPATLDDLIK
CHHHCCCCCCCCCCCCCEEEEEEEECCEEEEEEEEEEEEEEEEECCCCCCCCCCHHHHHH
LGKEVGFQYDVNNFYFSFAFIAAYGGYVFKDTGGGLDPNDIGLNNEGAKKGLELIKDFVQ
HHHHCCCEEECCHHHHHHHHHHHHCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
KYKFMPPDINYDMAKGNFQSGKIGLYISGPWDVDGFKKANVPFKVAPLPKIDGKPTPSFA
HHCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCEECCCCEEECCCCCCCCCCCCCCC
GVQAAFVSANSKHQKEAWDLMKYLVENTGLPLFETGNRIPVIKSLLDNPEVKGNEILSAF
CCEEEEECCCCCHHHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHCCCCCCHHHHHHHH
AEQAQHAIPMPNIPQMSQVWTPAGNALQLITSGKVPVDKAADDMVKQIKQGIATMQ
HHHHHHCCCCCCCCCHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; maltose [Periplasm]; H2O [C]

Specific reaction: ATP + maltose [Periplasm] + H2O = ADP + phosphate + maltose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]