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The map label for this gene is FruB [H]

Identifier: 20808237

GI number: 20808237

Start: 1770771

End: 1771037

Strand: Reverse

Name: FruB [H]

Synonym: TTE1820

Alternate gene names: 20808237

Gene position: 1771037-1770771 (Counterclockwise)

Preceding gene: 20808238

Following gene: 20808236

Centisome position: 65.85

GC content: 39.33

Gene sequence:

>267_bases
ATGAAAGAAGTTACTATTGAAATCAAAAATAAGACAGGGCTTCATGCAAGACCTGCGGCTTTATTTGTCCAAACAGCCAG
TAAATTTTCTTCCCAGATTTGGGTAGAAAAAGATAATAAAAAGGTAAATGCTAAAAGCATTATGGGAATAATGTCTCTAG
GGGTCTCCCAGGGCAATGTAGTGAAGTTGAGTGCTGAGGGAGACGATGAGGAGGAGGCTATAAAAGCTTTAGTTGACCTC
ATCGAATCAAAGTTCGGTGAAGAGTAA

Upstream 100 bases:

>100_bases
GCAAAATGTTGGTTCCTCCTGTGGGCAAGTCAGGGGTGAACCACAGGCTTAGGAAAATTGAAGAAATTTCAAACAAATTA
AAAGAGAGGAGAGTTCAGTA

Downstream 100 bases:

>100_bases
ACAAAAAAGGTTTCTTTTCAGAAACCTTTTTTGTGTAAAAGGATGATGGGAATGACAAAAAATGAAATGGTCTTGGAGTA
CATAAAGAAGCTGCCAGAAG

Product: phosphotransferase system, HPr-related proteins

Products: D-sorbitol 6-phosphate [Cytoplasm]; pyruvate; alpha,alpha-trehalose 6-phosphate [Cytoplasm]; mannitol-1-phosphate [Cytoplasm]; D-glucosamine-6-phosphate [Cytoplasm]; N-acetyl-D-glucosamine-6-phosphate [Cytoplasm]; mannose-6-phosphate [Cytoplasm]; galactitol-1-phosphate [Cytoplasm]; fructose-6-phosphate [Cytoplasm]; fructose-1-phosphate [Cytoplasm]; glucose-6-phosphate [Cytoplasm]; mannitol-1-phosphate [Cytoplasm]; diacetylchitobiose-6-phosphate [Cytoplasm]; cellobiose-6-phosphate [Cytoplasm]; salicin-6-phosphate [Cytoplasm]; arbutin-6-phosphate [Cytoplasm] [C]

Alternate protein names: Catabolite repression HPr [H]

Number of amino acids: Translated: 88; Mature: 88

Protein sequence:

>88_residues
MKEVTIEIKNKTGLHARPAALFVQTASKFSSQIWVEKDNKKVNAKSIMGIMSLGVSQGNVVKLSAEGDDEEEAIKALVDL
IESKFGEE

Sequences:

>Translated_88_residues
MKEVTIEIKNKTGLHARPAALFVQTASKFSSQIWVEKDNKKVNAKSIMGIMSLGVSQGNVVKLSAEGDDEEEAIKALVDL
IESKFGEE
>Mature_88_residues
MKEVTIEIKNKTGLHARPAALFVQTASKFSSQIWVEKDNKKVNAKSIMGIMSLGVSQGNVVKLSAEGDDEEEAIKALVDL
IESKFGEE

Specific function: Involved in carbon catabolite repression (CCR) [H]

COG id: COG1925

COG function: function code G; Phosphotransferase system, HPr-related proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HPr domain [H]

Homologues:

Organism=Escherichia coli, GI1788755, Length=80, Percent_Identity=48.75, Blast_Score=78, Evalue=9e-17,
Organism=Escherichia coli, GI1788494, Length=83, Percent_Identity=38.5542168674699, Blast_Score=69, Evalue=6e-14,
Organism=Escherichia coli, GI226510935, Length=86, Percent_Identity=39.5348837209302, Blast_Score=62, Evalue=7e-12,

Paralogues:

None

Copy number: 4180 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). 2100 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). 3235 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005698
- InterPro:   IPR000032
- InterPro:   IPR002114 [H]

Pfam domain/function: PF00381 PTS-HPr [H]

EC number: NA

Molecular weight: Translated: 9607; Mature: 9607

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: PS00369 PTS_HPR_HIS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEVTIEIKNKTGLHARPAALFVQTASKFSSQIWVEKDNKKVNAKSIMGIMSLGVSQGNV
CCEEEEEECCCCCCCCCCHHHHHHHHHHHCCEEEEECCCCEECHHHHHHHHHCCCCCCCE
VKLSAEGDDEEEAIKALVDLIESKFGEE
EEEECCCCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKEVTIEIKNKTGLHARPAALFVQTASKFSSQIWVEKDNKKVNAKSIMGIMSLGVSQGNV
CCEEEEEECCCCCCCCCCHHHHHHHHHHHCCEEEEECCCCEECHHHHHHHHHCCCCCCCE
VKLSAEGDDEEEAIKALVDLIESKFGEE
EEEECCCCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: sorbitol [Periplasm]; phosphoenolpyruvate; trehalose [Periplasm]; mannitol [Periplasm]; glucosamine [Periplasm]; N-acetyl-D-glucosamine [Periplasm]; mannose [Periplasm]; galactitol [Periplasm]; fructose [Periplasm]; beta-D-glucose [Periplasm]; diacetylchitobiose [Periplasm]; cellobiose [Periplasm]; salicin [Periplasm]; arbutin [Periplasm] [C]

Specific reaction: phosphoenolpyruvate + sorbitol [Periplasm] = D-sorbitol 6-phosphate [Cytoplasm] + pyruvate phosphoenolpyruvate + trehalose [Periplasm] = alpha,alpha-trehalose 6-phosphate [Cytoplasm] + pyruvate phosphoenolpyruvate + mannitol [Periplasm] = mannitol-1-phosp

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]