Click here to switch to the map view.

The map label for this gene is NfnB [C]

Identifier: 20808224

GI number: 20808224

Start: 1753704

End: 1754204

Strand: Direct

Name: NfnB [C]

Synonym: TTE1807

Alternate gene names: 20808224

Gene position: 1753704-1754204 (Clockwise)

Preceding gene: 20808222

Following gene: 20808225

Centisome position: 65.21

GC content: 37.72

Gene sequence:

>501_bases
ATGTTGGAGGCTTTAGAAGTTTTAAAAAAGAGAAGAAGTGTAAGAAAATACATTGATAAACCCATCCCAAAAGAAATTTT
AGAAGATATCATAGACTGTGCAAGGCTTGCACCTAGTGGAAACAATGCTCAGCCATGGCACTTCATTGTCATAACAGACA
AAGAAAAGCTCAAATTCATAGCAGAAAAAGCAACTTATGGAGACTTTATTAAGAACGCAGCAGCCTGTGTGATAGTATAC
TGCGAAAAAGACAATAAGCATCATTTAGAAGACGGTTCCGCTGCTACGCAAAACATACTGCTTGCAGCTACAGCCTACGG
AATAGGGTCCTGTTGGGTAGCGGGTTATAATAGAAGTTATGAAAAGGAAATAAATGAATACTTAAACGTGCCTGATAATC
TCCGCATGATATCAATAATATCGCTCGGTTACCCTGCTGAAGATCCACAACCCAAATACAAAAAAGACCTTTCTGAAGTT
TTACACTGGGAAAAATTTTAA

Upstream 100 bases:

>100_bases
CCATAAAACTATATTGCACAAGTTTAGCAAAGTTAATTCCTCTCACAATCTTTTTTGAGAATTACCCCTCATAGGATATA
ATATTATCTGGGAGGTGAAA

Downstream 100 bases:

>100_bases
GAGAAAGAGGGGTTAAAATTGTCAAAAGTAGTTGAACGTTTTTTGAAATATGTAAAATACCACACAACTTCAGATGAAAA
TTCCAACACTTTCCCGAGTA

Product: nitroreductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 166; Mature: 166

Protein sequence:

>166_residues
MLEALEVLKKRRSVRKYIDKPIPKEILEDIIDCARLAPSGNNAQPWHFIVITDKEKLKFIAEKATYGDFIKNAAACVIVY
CEKDNKHHLEDGSAATQNILLAATAYGIGSCWVAGYNRSYEKEINEYLNVPDNLRMISIISLGYPAEDPQPKYKKDLSEV
LHWEKF

Sequences:

>Translated_166_residues
MLEALEVLKKRRSVRKYIDKPIPKEILEDIIDCARLAPSGNNAQPWHFIVITDKEKLKFIAEKATYGDFIKNAAACVIVY
CEKDNKHHLEDGSAATQNILLAATAYGIGSCWVAGYNRSYEKEINEYLNVPDNLRMISIISLGYPAEDPQPKYKKDLSEV
LHWEKF
>Mature_166_residues
MLEALEVLKKRRSVRKYIDKPIPKEILEDIIDCARLAPSGNNAQPWHFIVITDKEKLKFIAEKATYGDFIKNAAACVIVY
CEKDNKHHLEDGSAATQNILLAATAYGIGSCWVAGYNRSYEKEINEYLNVPDNLRMISIISLGYPAEDPQPKYKKDLSEV
LHWEKF

Specific function: Reduction Of Nitroaromatic Compounds Using NADH. Reduces Nitrofurazone By A Ping-Pong Bi-Bi Mechanism Possibly To Generate A Two-Electron Transfer Product. Major Component Of The Oxygen- Insensitive Nitroreductase Activity In E.Coli. [C]

COG id: COG0778

COG function: function code C; Nitroreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitroreductase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000415 [H]

Pfam domain/function: PF00881 Nitroreductase [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 18946; Mature: 18946

Theoretical pI: Translated: 6.92; Mature: 6.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLEALEVLKKRRSVRKYIDKPIPKEILEDIIDCARLAPSGNNAQPWHFIVITDKEKLKFI
CCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEEEECHHHHHHH
AEKATYGDFIKNAAACVIVYCEKDNKHHLEDGSAATQNILLAATAYGIGSCWVAGYNRSY
HHHHHHHHHHHCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCHHEEECCCCCH
EKEINEYLNVPDNLRMISIISLGYPAEDPQPKYKKDLSEVLHWEKF
HHHHHHHCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLEALEVLKKRRSVRKYIDKPIPKEILEDIIDCARLAPSGNNAQPWHFIVITDKEKLKFI
CCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEEEECHHHHHHH
AEKATYGDFIKNAAACVIVYCEKDNKHHLEDGSAATQNILLAATAYGIGSCWVAGYNRSY
HHHHHHHHHHHCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCHHEEECCCCCH
EKEINEYLNVPDNLRMISIISLGYPAEDPQPKYKKDLSEVLHWEKF
HHHHHHHCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9389475 [H]