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The map label for this gene is murD

Identifier: 20808074

GI number: 20808074

Start: 1596571

End: 1597923

Strand: Reverse

Name: murD

Synonym: TTE1647

Alternate gene names: 20808074

Gene position: 1597923-1596571 (Counterclockwise)

Preceding gene: 20808075

Following gene: 20808073

Centisome position: 59.41

GC content: 39.25

Gene sequence:

>1353_bases
ATGGAATTGAAAGAAAAAAGAGTATTTGTAGCAGGGTTAGGCGTAAGTGGTGTAGCACTCTGCCGTGTGCTTGTAAATCT
GGGAGCAAATGTAATAGCTTATGACCGGAAAAATGAAATAGCCTTAAAAGAAGCTTTAGAGGAGTTAAAGGATTTGCCTG
TAGAAATAAAACTGGGAGAGTTTAAGGAAGAGTTTTTGAAAGGAATAGAGCTGGTAGTTTTAAGTCCAGGCATCTCTCTC
GAATCGGAAATAGTAAAAAAGGCTAAAGACATGGGACTTGAAATACTGGGAGAGGTAGAACTAGCTTATAGGCTTTCAAA
AGCCCCTATTTATGCAATCACAGGTACAAATGGAAAGACTACTACAACTTCTCTGTTAGGCGAGATGTTTAGAAACGCTG
GAAGGAAGGTGTATGTAGCTGGCAATATAGGGTATCCTCTCATCTATGCCGCTTTAGAAGCGGGACCTAATGACCATATT
GTAGCAGAGATCAGCAGTTTTCAGCTTGAAACTGTAAAAGAATTTAGGCCTAAGATAAGCTGTATAATAAACATAACGCC
TGACCATCTTGACAGGCACAAGACCTTTGAAAACTATGCAAATATAAAAGGAAGAATTTTTGAAAACCAAAGGGAAGAAG
AATACGTAGTTTTAAACTATGACGACCCTGTCACATGGGGGTTGAAAGAAAGGGCAAAAGCGAAAGTTTTTCCTTTCAGT
AGAAAGAAAGTCCTGGAAAATGGAGCGTATGTAAAAGAAGGCTTTTTATACTTACAGAATAAGAAAGTGATTAAAGTTGA
GGATATATACATTCCCGGAGAGCACAATTTAGAAAATGCATTGGCGGCTTCATCGGTAGCTTATCTTTCTGGAATTGAGG
TTGATGCTATTGAGACGACTTTGAGAACTTTCAAGGGTGTGGAACATAGAATAGAGTTTGTAGCGGAAATAGAGGGGATA
AAGTTTTACAACGATTCTAAAGGCACCAATCCCGACGCATCTATAAAGGCTATTCAGGCTCTTAAAACTCCTATAGTCCT
AATTGCAGGCGGTTATGACAAGGGAAGTGAGTTTGACGAGTTTGTAAAGACATTTGATAAGAAAGTGAGAAAGTTAATCC
TTATAGGGCAGACTGCTCAAAAGATAAAGAAAACTGCTCTTAAGTATTCATACCCTGAAGAAGACATATTTTTGGTGGAT
TCTTTAGAAGAGGCTGTGAGAAAAGCTTATGAGGTGGCAGAAAAGGGGGATAGCGTTCTTCTGTCGCCGGCCTGTGCTAG
CTGGGACATGTTTAAAAACTTCGAAGAGAGAGGCAAAGCTTTTAAAAAGGCTGTAATGGATTTGAGGAGGTAA

Upstream 100 bases:

>100_bases
TTCGGGTTGGAAAGAAGAGAAAGTTGTATACAGCTTTTGGTTGGTTACATTGATTGCTTTATTTGTATCTTTTTACAGTC
TGAGTTAAGGAGTGCAGTGT

Downstream 100 bases:

>100_bases
AAAGATGAAGAAAAAGCCCCCTGTGGATTACGGAATTTTGCTTGTGGTGATGATTTTAGTGGCAATAGGGGTGGTAATGG
TTTTTAGTGCAAGTGCTGCT

Product: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Number of amino acids: Translated: 450; Mature: 450

Protein sequence:

>450_residues
MELKEKRVFVAGLGVSGVALCRVLVNLGANVIAYDRKNEIALKEALEELKDLPVEIKLGEFKEEFLKGIELVVLSPGISL
ESEIVKKAKDMGLEILGEVELAYRLSKAPIYAITGTNGKTTTTSLLGEMFRNAGRKVYVAGNIGYPLIYAALEAGPNDHI
VAEISSFQLETVKEFRPKISCIINITPDHLDRHKTFENYANIKGRIFENQREEEYVVLNYDDPVTWGLKERAKAKVFPFS
RKKVLENGAYVKEGFLYLQNKKVIKVEDIYIPGEHNLENALAASSVAYLSGIEVDAIETTLRTFKGVEHRIEFVAEIEGI
KFYNDSKGTNPDASIKAIQALKTPIVLIAGGYDKGSEFDEFVKTFDKKVRKLILIGQTAQKIKKTALKYSYPEEDIFLVD
SLEEAVRKAYEVAEKGDSVLLSPACASWDMFKNFEERGKAFKKAVMDLRR

Sequences:

>Translated_450_residues
MELKEKRVFVAGLGVSGVALCRVLVNLGANVIAYDRKNEIALKEALEELKDLPVEIKLGEFKEEFLKGIELVVLSPGISL
ESEIVKKAKDMGLEILGEVELAYRLSKAPIYAITGTNGKTTTTSLLGEMFRNAGRKVYVAGNIGYPLIYAALEAGPNDHI
VAEISSFQLETVKEFRPKISCIINITPDHLDRHKTFENYANIKGRIFENQREEEYVVLNYDDPVTWGLKERAKAKVFPFS
RKKVLENGAYVKEGFLYLQNKKVIKVEDIYIPGEHNLENALAASSVAYLSGIEVDAIETTLRTFKGVEHRIEFVAEIEGI
KFYNDSKGTNPDASIKAIQALKTPIVLIAGGYDKGSEFDEFVKTFDKKVRKLILIGQTAQKIKKTALKYSYPEEDIFLVD
SLEEAVRKAYEVAEKGDSVLLSPACASWDMFKNFEERGKAFKKAVMDLRR
>Mature_450_residues
MELKEKRVFVAGLGVSGVALCRVLVNLGANVIAYDRKNEIALKEALEELKDLPVEIKLGEFKEEFLKGIELVVLSPGISL
ESEIVKKAKDMGLEILGEVELAYRLSKAPIYAITGTNGKTTTTSLLGEMFRNAGRKVYVAGNIGYPLIYAALEAGPNDHI
VAEISSFQLETVKEFRPKISCIINITPDHLDRHKTFENYANIKGRIFENQREEEYVVLNYDDPVTWGLKERAKAKVFPFS
RKKVLENGAYVKEGFLYLQNKKVIKVEDIYIPGEHNLENALAASSVAYLSGIEVDAIETTLRTFKGVEHRIEFVAEIEGI
KFYNDSKGTNPDASIKAIQALKTPIVLIAGGYDKGSEFDEFVKTFDKKVRKLILIGQTAQKIKKTALKYSYPEEDIFLVD
SLEEAVRKAYEVAEKGDSVLLSPACASWDMFKNFEERGKAFKKAVMDLRR

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA)

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family

Homologues:

Organism=Escherichia coli, GI1786276, Length=451, Percent_Identity=34.3680709534368, Blast_Score=222, Evalue=4e-59,
Organism=Escherichia coli, GI1786279, Length=258, Percent_Identity=29.0697674418605, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI1786274, Length=255, Percent_Identity=27.4509803921569, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURD_THETN (Q8R9G4)

Other databases:

- EMBL:   AE008691
- RefSeq:   NP_623245.1
- ProteinModelPortal:   Q8R9G4
- SMR:   Q8R9G4
- GeneID:   996201
- GenomeReviews:   AE008691_GR
- KEGG:   tte:TTE1647
- NMPDR:   fig|273068.3.peg.1629
- HOGENOM:   HBG750024
- OMA:   VKYYNDS
- ProtClustDB:   PRK14106
- BioCyc:   TTEN273068:TTE1647-MONOMER
- BRENDA:   6.3.2.9
- GO:   GO:0005737
- HAMAP:   MF_00639
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762
- Gene3D:   G3DSA:3.90.190.20
- Gene3D:   G3DSA:3.40.1190.10
- Gene3D:   G3DSA:3.40.50.720
- TIGRFAMs:   TIGR01087

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M; SSF53244 Mur_ligase_C; SSF53623 Mur_ligase_cen

EC number: =6.3.2.9

Molecular weight: Translated: 50442; Mature: 50442

Theoretical pI: Translated: 6.71; Mature: 6.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELKEKRVFVAGLGVSGVALCRVLVNLGANVIAYDRKNEIALKEALEELKDLPVEIKLGE
CCCCCCEEEEEECCHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHCCCEEEEECH
FKEEFLKGIELVVLSPGISLESEIVKKAKDMGLEILGEVELAYRLSKAPIYAITGTNGKT
HHHHHHCCCEEEEECCCCCHHHHHHHHHHHCCHHEEHHHHHHEEECCCCEEEEECCCCCC
TTTSLLGEMFRNAGRKVYVAGNIGYPLIYAALEAGPNDHIVAEISSFQLETVKEFRPKIS
HHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHCCCCCCEEEEEHHHHHHHHHHCCCCEE
CIINITPDHLDRHKTFENYANIKGRIFENQREEEYVVLNYDDPVTWGLKERAKAKVFPFS
EEEECCHHHHHHHHHHHHHHCCCEEEECCCCCCCEEEEECCCCCCCCHHHCCCCCCCCCH
RKKVLENGAYVKEGFLYLQNKKVIKVEDIYIPGEHNLENALAASSVAYLSGIEVDAIETT
HHHHHHCCCEEEECEEEEECCCEEEEEEEEECCCCCHHHHHHHHHHHHHCCCCHHHHHHH
LRTFKGVEHRIEFVAEIEGIKFYNDSKGTNPDASIKAIQALKTPIVLIAGGYDKGSEFDE
HHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCHHHHHHHHHCCCEEEEECCCCCCCCHHH
FVKTFDKKVRKLILIGQTAQKIKKTALKYSYPEEDIFLVDSLEEAVRKAYEVAEKGDSVL
HHHHHHHHHHHEEEECHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHCCCCEE
LSPACASWDMFKNFEERGKAFKKAVMDLRR
EECCCCCHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MELKEKRVFVAGLGVSGVALCRVLVNLGANVIAYDRKNEIALKEALEELKDLPVEIKLGE
CCCCCCEEEEEECCHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHCCCEEEEECH
FKEEFLKGIELVVLSPGISLESEIVKKAKDMGLEILGEVELAYRLSKAPIYAITGTNGKT
HHHHHHCCCEEEEECCCCCHHHHHHHHHHHCCHHEEHHHHHHEEECCCCEEEEECCCCCC
TTTSLLGEMFRNAGRKVYVAGNIGYPLIYAALEAGPNDHIVAEISSFQLETVKEFRPKIS
HHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHCCCCCCEEEEEHHHHHHHHHHCCCCEE
CIINITPDHLDRHKTFENYANIKGRIFENQREEEYVVLNYDDPVTWGLKERAKAKVFPFS
EEEECCHHHHHHHHHHHHHHCCCEEEECCCCCCCEEEEECCCCCCCCHHHCCCCCCCCCH
RKKVLENGAYVKEGFLYLQNKKVIKVEDIYIPGEHNLENALAASSVAYLSGIEVDAIETT
HHHHHHCCCEEEECEEEEECCCEEEEEEEEECCCCCHHHHHHHHHHHHHCCCCHHHHHHH
LRTFKGVEHRIEFVAEIEGIKFYNDSKGTNPDASIKAIQALKTPIVLIAGGYDKGSEFDE
HHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCHHHHHHHHHCCCEEEEECCCCCCCCHHH
FVKTFDKKVRKLILIGQTAQKIKKTALKYSYPEEDIFLVDSLEEAVRKAYEVAEKGDSVL
HHHHHHHHHHHEEEECHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHCCCCEE
LSPACASWDMFKNFEERGKAFKKAVMDLRR
EECCCCCHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11997336