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The map label for this gene is Tdh2 [H]

Identifier: 20808020

GI number: 20808020

Start: 1547793

End: 1548746

Strand: Reverse

Name: Tdh2 [H]

Synonym: TTE1591

Alternate gene names: 20808020

Gene position: 1548746-1547793 (Counterclockwise)

Preceding gene: 20808021

Following gene: 20808019

Centisome position: 57.59

GC content: 39.41

Gene sequence:

>954_bases
GTGAAAGCCCTTTATTTTGACGGAAAGCTTAAGCTAAAAGACCTTCCAAAGCCTGAAATAGAAGAAGGAGAATCGTTGAT
AAAGGTTTTACTGGCTTCTGTGTGTAACACTGATATAGAGATAATGAAAGGGTATAAAAATTTTAAAGGAGTACTTGGGC
ACGAATTCGTAGGAATTGTTGAAGATTCTTCTAATCCGCAATTGATTGGTAAAAGAGTGGTTGCGGATATCAACATAGCT
TGTGGTGAGTGCGACCTGTGCAAGGAAGGGCTTTTCCATCACTGCAGAAATAGAAAGGTCTTGGGTATATTCGAAAAGGA
TGGAGCTTTTGCGGAGTATATTACGCTTCCTAATCGCAATCTTTTCATTGTGCCTGATAGCATTAAAGATGAAGAAGCTG
TTTTTACTGAGCCTTTGGCGGCTGCCTTAGAAATTCTTGAGATGTATCATATAAAACCTACTGATAAAGTTGTAGTCATA
GGTGATGGCAAACTTTCTCAGTTTATAACGCAGGTGGTAGCTCTTACAGGATGTGACCTTTATGTGGTCGGTAAACATGA
GGAAAAACTTGAGCACCTCAAAAATAAAGCTAATACTCTTCTTTTGGAAGAAGTTGAAAGGCTTGAGACTAAAAGCGTGT
TTGACGTCGCTATAGAATGTACTGGCAATGAGGACGGATTGAAACTTGCTCAGCAGCTGGTTAAACCTACTGGCACAGTT
ATTTTGAAGAGCACATACAATGCTTATGCTACTTTGAATCCCACTGATTGGGTGGTGAATGAGATAAAATTGATAGGTAC
AAGGTGCGGTCCTATGGCGTCTGCCTTGAGGCTTCTTGAAAAGAGGTTTGTCACTACTGATTACCTCATAAGCGGTTTTT
ATAAGTTAGAGCAGTATGAAGAGGCTTTTTCTCCAAAAAATACTTTTAAGGCAGTTTTTGACTTAAGAGGTTAG

Upstream 100 bases:

>100_bases
GAACCTGTGCTTTTATCTTCTGAAGAGATGGAGCTTATGATGGAGAAGTTTAAAACTTATGGGCAGGTAAGAAAATAAAA
AATTTAAAAGGAGTGAAAAA

Downstream 100 bases:

>100_bases
GAGGAGTTGGAATGAAGGAGATTAAGACAATGGAATTTAGAGATGGCGTGCTTTATCTGATAGACCAGAGGAAACTTCCC
TTAAGTTATGAATTTTTTGA

Product: threonine dehydrogenase and related Zn-dependent dehydrogenase

Products: NA

Alternate protein names: DOIA dehydrogenase [H]

Number of amino acids: Translated: 317; Mature: 317

Protein sequence:

>317_residues
MKALYFDGKLKLKDLPKPEIEEGESLIKVLLASVCNTDIEIMKGYKNFKGVLGHEFVGIVEDSSNPQLIGKRVVADINIA
CGECDLCKEGLFHHCRNRKVLGIFEKDGAFAEYITLPNRNLFIVPDSIKDEEAVFTEPLAAALEILEMYHIKPTDKVVVI
GDGKLSQFITQVVALTGCDLYVVGKHEEKLEHLKNKANTLLLEEVERLETKSVFDVAIECTGNEDGLKLAQQLVKPTGTV
ILKSTYNAYATLNPTDWVVNEIKLIGTRCGPMASALRLLEKRFVTTDYLISGFYKLEQYEEAFSPKNTFKAVFDLRG

Sequences:

>Translated_317_residues
MKALYFDGKLKLKDLPKPEIEEGESLIKVLLASVCNTDIEIMKGYKNFKGVLGHEFVGIVEDSSNPQLIGKRVVADINIA
CGECDLCKEGLFHHCRNRKVLGIFEKDGAFAEYITLPNRNLFIVPDSIKDEEAVFTEPLAAALEILEMYHIKPTDKVVVI
GDGKLSQFITQVVALTGCDLYVVGKHEEKLEHLKNKANTLLLEEVERLETKSVFDVAIECTGNEDGLKLAQQLVKPTGTV
ILKSTYNAYATLNPTDWVVNEIKLIGTRCGPMASALRLLEKRFVTTDYLISGFYKLEQYEEAFSPKNTFKAVFDLRG
>Mature_317_residues
MKALYFDGKLKLKDLPKPEIEEGESLIKVLLASVCNTDIEIMKGYKNFKGVLGHEFVGIVEDSSNPQLIGKRVVADINIA
CGECDLCKEGLFHHCRNRKVLGIFEKDGAFAEYITLPNRNLFIVPDSIKDEEAVFTEPLAAALEILEMYHIKPTDKVVVI
GDGKLSQFITQVVALTGCDLYVVGKHEEKLEHLKNKANTLLLEEVERLETKSVFDVAIECTGNEDGLKLAQQLVKPTGTV
ILKSTYNAYATLNPTDWVVNEIKLIGTRCGPMASALRLLEKRFVTTDYLISGFYKLEQYEEAFSPKNTFKAVFDLRG

Specific function: Catalyzes the oxidation of 2-deoxy-scyllo-inosamine (DOIA) with NAD(+) or NADP(+), forming 3-amino-2,3-dideoxy-scyllo- inosose (amino-DOI) [H]

COG id: COG1063

COG function: function code ER; Threonine dehydrogenase and related Zn-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family. DOIA dehydrogenase subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788075, Length=344, Percent_Identity=26.453488372093, Blast_Score=102, Evalue=5e-23,
Organism=Escherichia coli, GI1787863, Length=314, Percent_Identity=26.7515923566879, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI226510992, Length=310, Percent_Identity=25.1612903225806, Blast_Score=98, Evalue=7e-22,
Organism=Escherichia coli, GI1790045, Length=275, Percent_Identity=26.5454545454545, Blast_Score=91, Evalue=7e-20,
Organism=Escherichia coli, GI1788407, Length=325, Percent_Identity=24.6153846153846, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1788073, Length=315, Percent_Identity=22.8571428571429, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1786825, Length=235, Percent_Identity=25.531914893617, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17562876, Length=333, Percent_Identity=22.8228228228228, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17562878, Length=323, Percent_Identity=21.0526315789474, Blast_Score=65, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6322619, Length=324, Percent_Identity=24.3827160493827, Blast_Score=70, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6319955, Length=324, Percent_Identity=24.3827160493827, Blast_Score=70, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6319257, Length=319, Percent_Identity=21.6300940438871, Blast_Score=66, Evalue=7e-12,
Organism=Saccharomyces cerevisiae, GI6323099, Length=338, Percent_Identity=23.6686390532544, Blast_Score=65, Evalue=1e-11,
Organism=Drosophila melanogaster, GI45551930, Length=307, Percent_Identity=27.0358306188925, Blast_Score=93, Evalue=2e-19,
Organism=Drosophila melanogaster, GI45550770, Length=307, Percent_Identity=27.0358306188925, Blast_Score=93, Evalue=2e-19,
Organism=Drosophila melanogaster, GI221457811, Length=307, Percent_Identity=27.0358306188925, Blast_Score=92, Evalue=3e-19,
Organism=Drosophila melanogaster, GI17137530, Length=317, Percent_Identity=22.397476340694, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI17737897, Length=328, Percent_Identity=23.1707317073171, Blast_Score=78, Evalue=8e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: NA

Molecular weight: Translated: 35440; Mature: 35440

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKALYFDGKLKLKDLPKPEIEEGESLIKVLLASVCNTDIEIMKGYKNFKGVLGHEFVGIV
CCEEEECCEEEECCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCEEEEE
EDSSNPQLIGKRVVADINIACGECDLCKEGLFHHCRNRKVLGIFEKDGAFAEYITLPNRN
ECCCCCHHHHHHHEEEEEEECCCCHHHHHHHHHHHCCCCEEEEEECCCCEEEEEECCCCE
LFIVPDSIKDEEAVFTEPLAAALEILEMYHIKPTDKVVVIGDGKLSQFITQVVALTGCDL
EEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHCCCCE
YVVGKHEEKLEHLKNKANTLLLEEVERLETKSVFDVAIECTGNEDGLKLAQQLVKPTGTV
EEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCHHHHHHHHHHCCCCCE
ILKSTYNAYATLNPTDWVVNEIKLIGTRCGPMASALRLLEKRFVTTDYLISGFYKLEQYE
EEEECCCEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EAFSPKNTFKAVFDLRG
HHCCCCHHHHHHHCCCC
>Mature Secondary Structure
MKALYFDGKLKLKDLPKPEIEEGESLIKVLLASVCNTDIEIMKGYKNFKGVLGHEFVGIV
CCEEEECCEEEECCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCEEEEE
EDSSNPQLIGKRVVADINIACGECDLCKEGLFHHCRNRKVLGIFEKDGAFAEYITLPNRN
ECCCCCHHHHHHHEEEEEEECCCCHHHHHHHHHHHCCCCEEEEEECCCCEEEEEECCCCE
LFIVPDSIKDEEAVFTEPLAAALEILEMYHIKPTDKVVVIGDGKLSQFITQVVALTGCDL
EEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHCCCCE
YVVGKHEEKLEHLKNKANTLLLEEVERLETKSVFDVAIECTGNEDGLKLAQQLVKPTGTV
EEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCHHHHHHHHHHCCCCCE
ILKSTYNAYATLNPTDWVVNEIKLIGTRCGPMASALRLLEKRFVTTDYLISGFYKLEQYE
EEEECCCEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EAFSPKNTFKAVFDLRG
HHCCCCHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA