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The map label for this gene is dapF

Identifier: 20807956

GI number: 20807956

Start: 1478580

End: 1479404

Strand: Reverse

Name: dapF

Synonym: TTE1514

Alternate gene names: 20807956

Gene position: 1479404-1478580 (Counterclockwise)

Preceding gene: 20807957

Following gene: 20807955

Centisome position: 55.01

GC content: 42.3

Gene sequence:

>825_bases
GTGAGATTTACTAAAATGCACGGTTTGGGCAATGACTTCATAGTGATTGAAGCAGTAGAGGGGGTTGATTACAGCGAACT
AGCTGTTAAACTCTGTGACAGGCACTTTGGCATAGGGGCAGATGGGCTTTTAGTGGTGGAGCCTTCACATATTGCTGATA
TCAAAATGAGGATTTTTAATGCGGATGGAAGCGAAGCTGAAATGTGCGGAAATGGTTCTAGATGTTTTGCGAAGTACGTC
TACGAGAAAGGCATAGTCTCTAAACAGAAGATGACTGTAGAAACTCTGGCAGGCGTAATAATGCCAGAACTGTTTGTAGA
AAATGGCAAAATAAAGAGTGTGAAAGTCTACATGGGAAGTCCTATTTTTGAAAGCAGTAAAATACCCGTAAAGAGCGAAA
AACAGAAATTCATAGATGAGCCAGTTAAGATAGATGGCAAAACCTATAGATTGTCATCTGTCAGAGTAGGAGTGCCTCAC
ACAATTTTGTTTGTCAGCTCTTTTGAGGAAAGTTTTATGAAAGAGCTGGGACCCAAAATTGAAAAAAGCTCCCTTTTCCC
CGAGGGGACGAATGTGGACTTTGTCAAGGTAGAGGATGAGGAAAATATATCTGTAAGGACTTGGGAGAGGGGAGTAGGGC
TCACTCTGGCGTGTGGAAGCGGGGCTTCGGCTTCTGCTGTTGTCTCATCTTTATTAGGAAGGACAAGAAGAAGTGTAAAT
GTGCATTTTAAGGCAGGAGTGCTGCTTGTTGAATGGAAAGAGGACAACAGCATCTATTTAAGCGGAGAAGTAGAAGAAGT
CTTCAGGGGAGAAATTGAAATTTGA

Upstream 100 bases:

>100_bases
TGTTTTATGACCGCTTTCCTCAATTTTTCTTAAAATATCTAACCAAATTGACCTTTTTTATGTTATAATATCTTTGAAGA
TTAGAAGGAGTGGTATTAAA

Downstream 100 bases:

>100_bases
GTTTTACGTTGTTTGAAATTTGACTATAATAAGAAAAAGGTGGTTTTGATGATAAAGAGCATGACGGGGTATGGACGAAG
CGACATTCGAGATAGAGGAT

Product: diaminopimelate epimerase

Products: NA

Alternate protein names: DAP epimerase

Number of amino acids: Translated: 274; Mature: 274

Protein sequence:

>274_residues
MRFTKMHGLGNDFIVIEAVEGVDYSELAVKLCDRHFGIGADGLLVVEPSHIADIKMRIFNADGSEAEMCGNGSRCFAKYV
YEKGIVSKQKMTVETLAGVIMPELFVENGKIKSVKVYMGSPIFESSKIPVKSEKQKFIDEPVKIDGKTYRLSSVRVGVPH
TILFVSSFEESFMKELGPKIEKSSLFPEGTNVDFVKVEDEENISVRTWERGVGLTLACGSGASASAVVSSLLGRTRRSVN
VHFKAGVLLVEWKEDNSIYLSGEVEEVFRGEIEI

Sequences:

>Translated_274_residues
MRFTKMHGLGNDFIVIEAVEGVDYSELAVKLCDRHFGIGADGLLVVEPSHIADIKMRIFNADGSEAEMCGNGSRCFAKYV
YEKGIVSKQKMTVETLAGVIMPELFVENGKIKSVKVYMGSPIFESSKIPVKSEKQKFIDEPVKIDGKTYRLSSVRVGVPH
TILFVSSFEESFMKELGPKIEKSSLFPEGTNVDFVKVEDEENISVRTWERGVGLTLACGSGASASAVVSSLLGRTRRSVN
VHFKAGVLLVEWKEDNSIYLSGEVEEVFRGEIEI
>Mature_274_residues
MRFTKMHGLGNDFIVIEAVEGVDYSELAVKLCDRHFGIGADGLLVVEPSHIADIKMRIFNADGSEAEMCGNGSRCFAKYV
YEKGIVSKQKMTVETLAGVIMPELFVENGKIKSVKVYMGSPIFESSKIPVKSEKQKFIDEPVKIDGKTYRLSSVRVGVPH
TILFVSSFEESFMKELGPKIEKSSLFPEGTNVDFVKVEDEENISVRTWERGVGLTLACGSGASASAVVSSLLGRTRRSVN
VHFKAGVLLVEWKEDNSIYLSGEVEEVFRGEIEI

Specific function: Biosynthesis of lysine from aspartate semialdehyde; sixth step. [C]

COG id: COG0253

COG function: function code E; Diaminopimelate epimerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diaminopimelate epimerase family

Homologues:

Organism=Escherichia coli, GI87082334, Length=281, Percent_Identity=40.2135231316726, Blast_Score=193, Evalue=9e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DAPF_THETN (Q8R9S4)

Other databases:

- EMBL:   AE008691
- RefSeq:   NP_623127.1
- ProteinModelPortal:   Q8R9S4
- SMR:   Q8R9S4
- GeneID:   997930
- GenomeReviews:   AE008691_GR
- KEGG:   tte:TTE1514
- NMPDR:   fig|273068.3.peg.1474
- HOGENOM:   HBG399442
- OMA:   HTGIGFD
- ProtClustDB:   PRK00450
- BioCyc:   TTEN273068:TTE1514-MONOMER
- BRENDA:   5.1.1.7
- GO:   GO:0005737
- HAMAP:   MF_00197
- InterPro:   IPR001653
- InterPro:   IPR018510
- TIGRFAMs:   TIGR00652

Pfam domain/function: PF01678 DAP_epimerase

EC number: =5.1.1.7

Molecular weight: Translated: 30259; Mature: 30259

Theoretical pI: Translated: 5.48; Mature: 5.48

Prosite motif: PS01326 DAP_EPIMERASE

Important sites: ACT_SITE 69-69 ACT_SITE 218-218

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFTKMHGLGNDFIVIEAVEGVDYSELAVKLCDRHFGIGADGLLVVEPSHIADIKMRIFN
CCEEECCCCCCCEEEEEEECCCCHHHHHHHHHHHHCCCCCCCEEEECCCCCCEEEEEEEC
ADGSEAEMCGNGSRCFAKYVYEKGIVSKQKMTVETLAGVIMPELFVENGKIKSVKVYMGS
CCCCCHHHCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECC
PIFESSKIPVKSEKQKFIDEPVKIDGKTYRLSSVRVGVPHTILFVSSFEESFMKELGPKI
CCCCCCCCCCCCHHHHHCCCCEEECCCEEEEEEEEECCCEEEEEEEHHHHHHHHHHCCCC
EKSSLFPEGTNVDFVKVEDEENISVRTWERGVGLTLACGSGASASAVVSSLLGRTRRSVN
CHHCCCCCCCCEEEEEECCCCCCEEEEECCCCCEEEEECCCCCHHHHHHHHHHCCCCEEE
VHFKAGVLLVEWKEDNSIYLSGEVEEVFRGEIEI
EEEEEEEEEEEEECCCEEEEECCHHHHHCCCCCC
>Mature Secondary Structure
MRFTKMHGLGNDFIVIEAVEGVDYSELAVKLCDRHFGIGADGLLVVEPSHIADIKMRIFN
CCEEECCCCCCCEEEEEEECCCCHHHHHHHHHHHHCCCCCCCEEEECCCCCCEEEEEEEC
ADGSEAEMCGNGSRCFAKYVYEKGIVSKQKMTVETLAGVIMPELFVENGKIKSVKVYMGS
CCCCCHHHCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECC
PIFESSKIPVKSEKQKFIDEPVKIDGKTYRLSSVRVGVPHTILFVSSFEESFMKELGPKI
CCCCCCCCCCCCHHHHHCCCCEEECCCEEEEEEEEECCCEEEEEEEHHHHHHHHHHCCCC
EKSSLFPEGTNVDFVKVEDEENISVRTWERGVGLTLACGSGASASAVVSSLLGRTRRSVN
CHHCCCCCCCCEEEEEECCCCCCEEEEECCCCCEEEEECCCCCHHHHHHHHHHCCCCEEE
VHFKAGVLLVEWKEDNSIYLSGEVEEVFRGEIEI
EEEEEEEEEEEEECCCEEEEECCHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11997336