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The map label for this gene is tsf [H]

Identifier: 20807855

GI number: 20807855

Start: 1390984

End: 1391586

Strand: Reverse

Name: tsf [H]

Synonym: TTE1408

Alternate gene names: 20807855

Gene position: 1391586-1390984 (Counterclockwise)

Preceding gene: 20807856

Following gene: 20807854

Centisome position: 51.74

GC content: 39.97

Gene sequence:

>603_bases
ATGATATCTGCACAAGCTGTAAAAGAGCTGAGAGAGAGAACCGGCGCTGGAATGATGGATTGCAAAAACGCCTTGATTGA
AGCAAATGGAGATATAGAAAAAGCTATAGATATTCTAAGAGAAAAAGGGCTGGCTGCAGCAGCTAAGAAAGCGGGCAGAA
CTGCAAATGAAGGTTTGGTAGAAGCCTACATACATGGGGGCGGCAGAATTGGCGTGCTTGTAGAAGTGAACTGTGAGACA
GATTTTGTGGCAAATACAGAGGAATTTAGAAATTTTGTGAAAGAGATTTGCATGCAAATTGCTGCTGCAAATCCGAAGTA
CATCTCGAAAGAAGATGTGCCTCAAGAAGTATTGGAAAAAGAAAAAGAGATCTTAAGAGCTCAAGCTTTAAATGAAGGAA
AACCTGCCAATGTGATTGACAGAATAGTGGAAGGGCGATTGGAAAAATTCTATAAAGAAAATTGCCTTCTCGAGCAAGAG
TACATCAGAGACCCTGAGAAAACTGTAAAAGATTTATTGAACGAGATGATAGCAAAGCTTGGGGAGAATATTGTAATAAG
GAGATTTGCAAGGTTTGAGAGAGGAGAAGGCATAGAGAAATAA

Upstream 100 bases:

>100_bases
AGCAGTTTGAAGCAGCTGAAGAATAAAATACTCTTTTGGTGGTAGGGAAGCTTCCTTACCACTAAATTTATACTAAAAAG
CACAAGGAGGTACTTCTTGC

Downstream 100 bases:

>100_bases
AGGGACACTGATGTGTCCCCTTTTTATAGAAATTTCGAAATTTCAGAAGGATTTTTTGAAATTGTATAGAAACTTATATA
AGAATGGGGTGTTATTGTGT

Product: elongation factor Ts

Products: NA

Alternate protein names: EF-Ts [H]

Number of amino acids: Translated: 200; Mature: 200

Protein sequence:

>200_residues
MISAQAVKELRERTGAGMMDCKNALIEANGDIEKAIDILREKGLAAAAKKAGRTANEGLVEAYIHGGGRIGVLVEVNCET
DFVANTEEFRNFVKEICMQIAAANPKYISKEDVPQEVLEKEKEILRAQALNEGKPANVIDRIVEGRLEKFYKENCLLEQE
YIRDPEKTVKDLLNEMIAKLGENIVIRRFARFERGEGIEK

Sequences:

>Translated_200_residues
MISAQAVKELRERTGAGMMDCKNALIEANGDIEKAIDILREKGLAAAAKKAGRTANEGLVEAYIHGGGRIGVLVEVNCET
DFVANTEEFRNFVKEICMQIAAANPKYISKEDVPQEVLEKEKEILRAQALNEGKPANVIDRIVEGRLEKFYKENCLLEQE
YIRDPEKTVKDLLNEMIAKLGENIVIRRFARFERGEGIEK
>Mature_200_residues
MISAQAVKELRERTGAGMMDCKNALIEANGDIEKAIDILREKGLAAAAKKAGRTANEGLVEAYIHGGGRIGVLVEVNCET
DFVANTEEFRNFVKEICMQIAAANPKYISKEDVPQEVLEKEKEILRAQALNEGKPANVIDRIVEGRLEKFYKENCLLEQE
YIRDPEKTVKDLLNEMIAKLGENIVIRRFARFERGEGIEK

Specific function: Associates with the EF-Tu.GDP complex and induces the exchange of GDP to GTP. It remains bound to the aminoacyl-tRNA.EF- Tu.GTP complex up to the GTP hydrolysis stage on the ribosome [H]

COG id: COG0264

COG function: function code J; Translation elongation factor Ts

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the EF-Ts family [H]

Homologues:

Organism=Homo sapiens, GI291084500, Length=202, Percent_Identity=31.1881188118812, Blast_Score=75, Evalue=5e-14,
Organism=Escherichia coli, GI1786366, Length=127, Percent_Identity=48.0314960629921, Blast_Score=103, Evalue=7e-24,

Paralogues:

None

Copy number: 2670 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1100 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 4173 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 14563 Molecules/Cell In: Growth Phase, Gluco

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001816
- InterPro:   IPR014039
- InterPro:   IPR018101
- InterPro:   IPR009060
- InterPro:   IPR000449 [H]

Pfam domain/function: PF00889 EF_TS; PF00627 UBA [H]

EC number: NA

Molecular weight: Translated: 22439; Mature: 22439

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: PS01126 EF_TS_1 ; PS01127 EF_TS_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISAQAVKELRERTGAGMMDCKNALIEANGDIEKAIDILREKGLAAAAKKAGRTANEGLV
CCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHCCCCCHHHHH
EAYIHGGGRIGVLVEVNCETDFVANTEEFRNFVKEICMQIAAANPKYISKEDVPQEVLEK
HHHHCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHH
EKEILRAQALNEGKPANVIDRIVEGRLEKFYKENCLLEQEYIRDPEKTVKDLLNEMIAKL
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHCCCHHHHHHHHHHHHHHHH
GENIVIRRFARFERGEGIEK
CCHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MISAQAVKELRERTGAGMMDCKNALIEANGDIEKAIDILREKGLAAAAKKAGRTANEGLV
CCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHCCCCCHHHHH
EAYIHGGGRIGVLVEVNCETDFVANTEEFRNFVKEICMQIAAANPKYISKEDVPQEVLEK
HHHHCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHH
EKEILRAQALNEGKPANVIDRIVEGRLEKFYKENCLLEQEYIRDPEKTVKDLLNEMIAKL
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHCCCHHHHHHHHHHHHHHHH
GENIVIRRFARFERGEGIEK
CCHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA