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The map label for this gene is miaA

Identifier: 20807806

GI number: 20807806

Start: 1342726

End: 1343673

Strand: Direct

Name: miaA

Synonym: TTE1359

Alternate gene names: 20807806

Gene position: 1342726-1343673 (Clockwise)

Preceding gene: 20807805

Following gene: 20807807

Centisome position: 49.93

GC content: 36.18

Gene sequence:

>948_bases
ATGGCTATACAAATTGTTCTTATTGTAGGGCCTACTGCCACTGGAAAATCAAGATTGGCTGTTGATGTAGCAAAGAGATT
TAACGGTGAAGTAGTCTCTGCTGATTCCATGCAGATTTACAAATACATGGACATTGGCACAGCGAAAATTACAAAGGAAG
AGATGCAAGGCATACCTCACCACATGATAGACATAGTGGAACCTAACGAAGAATTTAGCGTAGCAGAATATGAAAAGAGA
GCAAAGGCTATCATTAAAGACATCCATGAAAGAGGAAAACTTCCCATAATTGTTGGAGGCACGGGACTTTACATAAATTC
CATCGTTTACATCATGCACTTTTCTGATTTTGAAGGCAGCAAGGAATTTAGAGAAAAGATGAAAAAACTCGCTGAAACTT
ACGGTACCAGTTATTTGTATGAAAAGTTAAAAGAAGTGGACCCAGAGGCAGCGAGGAAAATTCATCCCAATGATTTAAGG
AGAATTATAAGGGCCTTAGAAGTGTACGAATTTACGGGAAAACCTATTTCTCATTATCAGAAGATGAGCGGAATGCGAAA
AAATCCCGAATATGATCCGATAATGATAGGGTTAAACTATAAAAACAGGCAGCTTTTGTACGAAAAAATTAATAGAAGAG
TAGATGAGATGATAAAAAATAATTTAGTCGAAGAAGTGGTAAACTTGCTAAAAATCGGGTATAATAAATATGGCACTTCA
ATGCAGGCTTTAGGTTACAAAGAAATTGTCGAGTATTTAAATGGGGAAATTTCTTTAGAGGAAGCCGTAGAGAAAATCAA
AAAGGGGACAAGGAGGTATGCGAAGAGACAGATCACGTGGTTTAAGGCTTATGACTTTATCAAATGGTTTTTTGTAGATG
ACTATAAAACCTATGAGGAACTTCGAGAAAATATTTTTGAATACTTGGCAGGAAAATTGAAATTTTGA

Upstream 100 bases:

>100_bases
GAAAATCCTTATACCTGTCCACATGGCAGGCCTGTCATAATTTCCATGACTAAGACCCAGCTTGAGAAGATGTTTAAACG
AATCAAGTAAGGGTGATGGG

Downstream 100 bases:

>100_bases
TAGAATATAACTAAATGATACATATTAAAGAAGGGGGATAAAGATGGCAAGTTCAAAAGCAGCTATTAATTTACAAGACA
TCTTTTTAAATCAAGTGAGA

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase

Number of amino acids: Translated: 315; Mature: 314

Protein sequence:

>315_residues
MAIQIVLIVGPTATGKSRLAVDVAKRFNGEVVSADSMQIYKYMDIGTAKITKEEMQGIPHHMIDIVEPNEEFSVAEYEKR
AKAIIKDIHERGKLPIIVGGTGLYINSIVYIMHFSDFEGSKEFREKMKKLAETYGTSYLYEKLKEVDPEAARKIHPNDLR
RIIRALEVYEFTGKPISHYQKMSGMRKNPEYDPIMIGLNYKNRQLLYEKINRRVDEMIKNNLVEEVVNLLKIGYNKYGTS
MQALGYKEIVEYLNGEISLEEAVEKIKKGTRRYAKRQITWFKAYDFIKWFFVDDYKTYEELRENIFEYLAGKLKF

Sequences:

>Translated_315_residues
MAIQIVLIVGPTATGKSRLAVDVAKRFNGEVVSADSMQIYKYMDIGTAKITKEEMQGIPHHMIDIVEPNEEFSVAEYEKR
AKAIIKDIHERGKLPIIVGGTGLYINSIVYIMHFSDFEGSKEFREKMKKLAETYGTSYLYEKLKEVDPEAARKIHPNDLR
RIIRALEVYEFTGKPISHYQKMSGMRKNPEYDPIMIGLNYKNRQLLYEKINRRVDEMIKNNLVEEVVNLLKIGYNKYGTS
MQALGYKEIVEYLNGEISLEEAVEKIKKGTRRYAKRQITWFKAYDFIKWFFVDDYKTYEELRENIFEYLAGKLKF
>Mature_314_residues
AIQIVLIVGPTATGKSRLAVDVAKRFNGEVVSADSMQIYKYMDIGTAKITKEEMQGIPHHMIDIVEPNEEFSVAEYEKRA
KAIIKDIHERGKLPIIVGGTGLYINSIVYIMHFSDFEGSKEFREKMKKLAETYGTSYLYEKLKEVDPEAARKIHPNDLRR
IIRALEVYEFTGKPISHYQKMSGMRKNPEYDPIMIGLNYKNRQLLYEKINRRVDEMIKNNLVEEVVNLLKIGYNKYGTSM
QALGYKEIVEYLNGEISLEEAVEKIKKGTRRYAKRQITWFKAYDFIKWFFVDDYKTYEELRENIFEYLAGKLKF

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family

Homologues:

Organism=Homo sapiens, GI31581534, Length=314, Percent_Identity=34.7133757961783, Blast_Score=173, Evalue=2e-43,
Organism=Escherichia coli, GI1790613, Length=308, Percent_Identity=34.7402597402597, Blast_Score=199, Evalue=2e-52,
Organism=Caenorhabditis elegans, GI25144712, Length=306, Percent_Identity=33.0065359477124, Blast_Score=151, Evalue=4e-37,
Organism=Saccharomyces cerevisiae, GI6324848, Length=294, Percent_Identity=34.0136054421769, Blast_Score=183, Evalue=3e-47,
Organism=Drosophila melanogaster, GI24649830, Length=334, Percent_Identity=31.7365269461078, Blast_Score=166, Evalue=3e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAA_THETN (Q8R5S5)

Other databases:

- EMBL:   AE008691
- RefSeq:   NP_622977.1
- ProteinModelPortal:   Q8R5S5
- SMR:   Q8R5S5
- GeneID:   997773
- GenomeReviews:   AE008691_GR
- KEGG:   tte:TTE1359
- NMPDR:   fig|273068.3.peg.118
- HOGENOM:   HBG559459
- OMA:   VNADSMQ
- ProtClustDB:   PRK00091
- BioCyc:   TTEN273068:TTE1359-MONOMER
- BRENDA:   2.5.1.8
- HAMAP:   MF_00185
- InterPro:   IPR002627
- InterPro:   IPR018022
- PANTHER:   PTHR11088
- TIGRFAMs:   TIGR00174

Pfam domain/function: PF01715 IPPT

EC number: =2.5.1.75

Molecular weight: Translated: 36842; Mature: 36711

Theoretical pI: Translated: 9.31; Mature: 9.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIQIVLIVGPTATGKSRLAVDVAKRFNGEVVSADSMQIYKYMDIGTAKITKEEMQGIPH
CEEEEEEEECCCCCCCCHHHHHHHHHCCCCEEECCCHHHHHHHCCCHHHCCHHHHHCCCH
HMIDIVEPNEEFSVAEYEKRAKAIIKDIHERGKLPIIVGGTGLYINSIVYIMHFSDFEGS
HHHEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHEEEECCCCCC
KEFREKMKKLAETYGTSYLYEKLKEVDPEAARKIHPNDLRRIIRALEVYEFTGKPISHYQ
HHHHHHHHHHHHHHCHHHHHHHHHHCCHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHH
KMSGMRKNPEYDPIMIGLNYKNRQLLYEKINRRVDEMIKNNLVEEVVNLLKIGYNKYGTS
HHCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCC
MQALGYKEIVEYLNGEISLEEAVEKIKKGTRRYAKRQITWFKAYDFIKWFFVDDYKTYEE
HHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
LRENIFEYLAGKLKF
HHHHHHHHHHHHCCC
>Mature Secondary Structure 
AIQIVLIVGPTATGKSRLAVDVAKRFNGEVVSADSMQIYKYMDIGTAKITKEEMQGIPH
EEEEEEEECCCCCCCCHHHHHHHHHCCCCEEECCCHHHHHHHCCCHHHCCHHHHHCCCH
HMIDIVEPNEEFSVAEYEKRAKAIIKDIHERGKLPIIVGGTGLYINSIVYIMHFSDFEGS
HHHEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHEEEECCCCCC
KEFREKMKKLAETYGTSYLYEKLKEVDPEAARKIHPNDLRRIIRALEVYEFTGKPISHYQ
HHHHHHHHHHHHHHCHHHHHHHHHHCCHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHH
KMSGMRKNPEYDPIMIGLNYKNRQLLYEKINRRVDEMIKNNLVEEVVNLLKIGYNKYGTS
HHCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCC
MQALGYKEIVEYLNGEISLEEAVEKIKKGTRRYAKRQITWFKAYDFIKWFFVDDYKTYEE
HHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
LRENIFEYLAGKLKF
HHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11997336