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The map label for this gene is SpeE

Identifier: 20807781

GI number: 20807781

Start: 1318819

End: 1319652

Strand: Direct

Name: SpeE

Synonym: TTE1334

Alternate gene names: 20807781

Gene position: 1318819-1319652 (Clockwise)

Preceding gene: 20807780

Following gene: 20807782

Centisome position: 49.04

GC content: 37.05

Gene sequence:

>834_bases
GTGGAACTTTGGTTTACAGAACATCAAGATGAAAATTTGAGGTTTTCTATTAAGGTAAAGGAGACGCTAGTTGTTGAAAA
AACTCCTTATCAGCATCTAGCAATTTTGGACAGCTATCAATTTGGGAGGGTTTTAGTACTGGATGGAATCCTCCAGACTA
CGGAAAAAGACGAATTTGTGTACCATGAAATGATAGTCCATGTGCCTTTATTTACCCATAAAAATCCTAAAGATGTGCTT
ATAATAGGAGGAGGAGATGGAGGCTCAGTAAGGGAGGTTTTGAAGCATTCATCTGTTGAGAGGGTTGTTTTGGCTGAAAT
TGATGAGGCAGTTATAAGAAACTCAAAAAAATATTTGCCTACCATAAGCAGTGCTCTAGATGACCCCAGAGTAGAAATAA
TGATTGGAGATGGGATAAAATATGTCAATGAGCATAAAAACGAATTTGATGTAGTAATTGTTGACTCCACTGACCCAATA
GGGCCTGCAGTAGGACTTTTTACTTCAGACTTTTACAGAGCTGTATACGAGTGCCTCAGAGAAGATGGAATTATTGTAGC
TCAAACAGAATCGCCTTTTATATACGGAAAACTGATTAACAAAATAAGCAAGATGTTCAAGGAGATTTATCCGATAACAA
AAGCTTACATTGCTACAGTTCCCACATATCCCGGTAGCTTGTGGACATTTACAATGGGTTCTAAAAAATATGACCCAGAG
GAAGTCGATATAAATTCTATACCTAGAATTCAAACCAAGTATTATACACCGGAAATCCATAAGGCGGCTTTTGTGCTTCC
TAAATTTGTAAAAGATATTTTTGATGAGGTGTAA

Upstream 100 bases:

>100_bases
GGATTTTTTTTGTGAGATTGGCTTTGTGAGAGAAGGAAATTTTCAAGTTGTCAATTTAAAAAATTTTTTTAAAGAATAAA
AATTACTGGAGGTGCTTTTG

Downstream 100 bases:

>100_bases
AATGGGAGAGAGAGTAAATTTCATAGATACAGGAAAGTTTTTAACCGCTACTAAGGAATATGAGGAGGCAGACATTGTCT
TGGTAGGGGTACCTATGGAT

Product: spermidine synthase

Products: NA

Alternate protein names: Putrescine aminopropyltransferase 1; PAPT 1; SPDSY 1

Number of amino acids: Translated: 277; Mature: 277

Protein sequence:

>277_residues
MELWFTEHQDENLRFSIKVKETLVVEKTPYQHLAILDSYQFGRVLVLDGILQTTEKDEFVYHEMIVHVPLFTHKNPKDVL
IIGGGDGGSVREVLKHSSVERVVLAEIDEAVIRNSKKYLPTISSALDDPRVEIMIGDGIKYVNEHKNEFDVVIVDSTDPI
GPAVGLFTSDFYRAVYECLREDGIIVAQTESPFIYGKLINKISKMFKEIYPITKAYIATVPTYPGSLWTFTMGSKKYDPE
EVDINSIPRIQTKYYTPEIHKAAFVLPKFVKDIFDEV

Sequences:

>Translated_277_residues
MELWFTEHQDENLRFSIKVKETLVVEKTPYQHLAILDSYQFGRVLVLDGILQTTEKDEFVYHEMIVHVPLFTHKNPKDVL
IIGGGDGGSVREVLKHSSVERVVLAEIDEAVIRNSKKYLPTISSALDDPRVEIMIGDGIKYVNEHKNEFDVVIVDSTDPI
GPAVGLFTSDFYRAVYECLREDGIIVAQTESPFIYGKLINKISKMFKEIYPITKAYIATVPTYPGSLWTFTMGSKKYDPE
EVDINSIPRIQTKYYTPEIHKAAFVLPKFVKDIFDEV
>Mature_277_residues
MELWFTEHQDENLRFSIKVKETLVVEKTPYQHLAILDSYQFGRVLVLDGILQTTEKDEFVYHEMIVHVPLFTHKNPKDVL
IIGGGDGGSVREVLKHSSVERVVLAEIDEAVIRNSKKYLPTISSALDDPRVEIMIGDGIKYVNEHKNEFDVVIVDSTDPI
GPAVGLFTSDFYRAVYECLREDGIIVAQTESPFIYGKLINKISKMFKEIYPITKAYIATVPTYPGSLWTFTMGSKKYDPE
EVDINSIPRIQTKYYTPEIHKAAFVLPKFVKDIFDEV

Specific function: Catalyzes the production of spermidine from putrescine and decarboxylated S-adenosylmethionine (dcSAM), which serves as an aminopropyl donor

COG id: COG0421

COG function: function code E; Spermidine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the spermidine/spermine synthase family

Homologues:

Organism=Homo sapiens, GI63253298, Length=284, Percent_Identity=39.0845070422535, Blast_Score=232, Evalue=2e-61,
Organism=Escherichia coli, GI1786312, Length=261, Percent_Identity=41.3793103448276, Blast_Score=218, Evalue=3e-58,
Organism=Caenorhabditis elegans, GI25154106, Length=267, Percent_Identity=43.8202247191011, Blast_Score=225, Evalue=2e-59,
Organism=Caenorhabditis elegans, GI212645616, Length=221, Percent_Identity=47.5113122171946, Blast_Score=213, Evalue=8e-56,
Organism=Saccharomyces cerevisiae, GI6325326, Length=282, Percent_Identity=41.1347517730496, Blast_Score=224, Evalue=1e-59,
Organism=Saccharomyces cerevisiae, GI6323175, Length=288, Percent_Identity=38.5416666666667, Blast_Score=202, Evalue=3e-53,
Organism=Drosophila melanogaster, GI24645443, Length=280, Percent_Identity=42.5, Blast_Score=233, Evalue=7e-62,
Organism=Drosophila melanogaster, GI21355941, Length=217, Percent_Identity=41.9354838709677, Blast_Score=178, Evalue=4e-45,
Organism=Drosophila melanogaster, GI21358269, Length=159, Percent_Identity=33.9622641509434, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24663238, Length=159, Percent_Identity=33.9622641509434, Blast_Score=77, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SPEE1_THETN (Q8RA94)

Other databases:

- EMBL:   AE008691
- RefSeq:   NP_622952.1
- ProteinModelPortal:   Q8RA94
- SMR:   Q8RA94
- GeneID:   997726
- GenomeReviews:   AE008691_GR
- KEGG:   tte:TTE1334
- NMPDR:   fig|273068.3.peg.1272
- HOGENOM:   HBG635113
- OMA:   FVAQTES
- ProtClustDB:   PRK00811
- BioCyc:   TTEN273068:TTE1334-MONOMER
- BRENDA:   2.5.1.16
- HAMAP:   MF_00198
- InterPro:   IPR001045
- PANTHER:   PTHR11558
- TIGRFAMs:   TIGR00417

Pfam domain/function: PF01564 Spermine_synth

EC number: =2.5.1.16

Molecular weight: Translated: 31642; Mature: 31642

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: PS01330 SPERMIDINE_SYNTHASE_1; PS51006 SPERMIDINE_SYNTHASE_2

Important sites: BINDING 31-31 BINDING 86-86 BINDING 106-106 BINDING 155-155 BINDING 158-158

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELWFTEHQDENLRFSIKVKETLVVEKTPYQHLAILDSYQFGRVLVLDGILQTTEKDEFV
CCCEECCCCCCCEEEEEEEEEEEEEECCCCHHEEEECCCCCCEEEEEHHHHHCCCCCHHH
YHEMIVHVPLFTHKNPKDVLIIGGGDGGSVREVLKHSSVERVVLAEIDEAVIRNSKKYLP
HHHHHHHEEEEECCCCCEEEEEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCC
TISSALDDPRVEIMIGDGIKYVNEHKNEFDVVIVDSTDPIGPAVGLFTSDFYRAVYECLR
CHHHHCCCCCEEEEECCCCHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHH
EDGIIVAQTESPFIYGKLINKISKMFKEIYPITKAYIATVPTYPGSLWTFTMGSKKYDPE
HCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCEEEEECCCCCCCCC
EVDINSIPRIQTKYYTPEIHKAAFVLPKFVKDIFDEV
CCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MELWFTEHQDENLRFSIKVKETLVVEKTPYQHLAILDSYQFGRVLVLDGILQTTEKDEFV
CCCEECCCCCCCEEEEEEEEEEEEEECCCCHHEEEECCCCCCEEEEEHHHHHCCCCCHHH
YHEMIVHVPLFTHKNPKDVLIIGGGDGGSVREVLKHSSVERVVLAEIDEAVIRNSKKYLP
HHHHHHHEEEEECCCCCEEEEEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCC
TISSALDDPRVEIMIGDGIKYVNEHKNEFDVVIVDSTDPIGPAVGLFTSDFYRAVYECLR
CHHHHCCCCCEEEEECCCCHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHH
EDGIIVAQTESPFIYGKLINKISKMFKEIYPITKAYIATVPTYPGSLWTFTMGSKKYDPE
HCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCEEEEECCCCCCCCC
EVDINSIPRIQTKYYTPEIHKAAFVLPKFVKDIFDEV
CCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11997336