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The map label for this gene is CheY7 [H]

Identifier: 20807753

GI number: 20807753

Start: 1295584

End: 1296366

Strand: Direct

Name: CheY7 [H]

Synonym: TTE1304

Alternate gene names: 20807753

Gene position: 1295584-1296366 (Clockwise)

Preceding gene: 20807752

Following gene: 20807754

Centisome position: 48.17

GC content: 39.97

Gene sequence:

>783_bases
TTGATTAAGAAGATTAGAATAGCCATTGCAGACGACAACAAAGAATTTGCTAACATTTTGGCTGAGTATTTGTCTGCTCA
GCAGAACATTGAAGTGGTAGGGGTTGCCAATGACGGCAACCAAGCTATTGAGATTATAAAAAATCAATCGCCAGACCTTT
TGATTTTAGACATTATAATGCCATATCTAGATGGCATAGGTGTGCTGGAAAAAATAAATGAAGAGCAGCTTAAGAAGCCC
AAAATTCTCATTCTGTCTGCAGTAGGGCAGGAGAAAATTACGCAAAGAGCCATAGCATTGGGAGCAGATTACTACGTGCT
CAAACCTTTTGACCTTGCAATGCTGTCAAAGAGGATCTTAGAACTGATGGAACCCGACTTGGACATTGTGTCAAGGACGG
TTTTCCATGTTGTGAAAGCGAAGAAAGATTACGATTTGGAGGCTATGATTACAGAGATCATCCACGAAGTCGGAGTACCT
GCCCACATAAAGGGATATATGTATTTAAGAGATGCGATTTCTCTTGTGATAAGCAATATGGAGTATTTGAATTCAGTCAC
AAAAATGCTGTATCCCAAGATAGCTGAAAAGTACAATACTACGCCGAGCAGAGTAGAGAGAGCTATAAGACACGCCATTG
AAGTAGCGTGGAGCAGAGGAAGGACAGAAGTTTTAAATGAACTTTTTGGCTATACTATAAACGATGAGAAAGGAAAACCC
ACCAACTCTGAATTTATTGCTCTTATCGCTGATAAGTTGAGGCTTGGAATGAAAGCAGGATAA

Upstream 100 bases:

>100_bases
TTTTATAAATGAATAAAAATTTTTACCAAAAAGGAGGAAATTCAACTATTATGTAGAATAAATATTATATACTAATATTA
GCAACGGGAGGTAAGAGTAA

Downstream 100 bases:

>100_bases
TTTTCTATTTCCTGCTTTTTATTTTTTGTTTATTTTATGTAAATTTTTTTTGTGTTATAATAGACAGGGAAGTTATACTC
CGGCAGGTGAGTATTTATGC

Product: CheY-like domain-containing protein

Products: protein L-glutamate+ methanol; methanol; Tapglu; methanol; Targlu; Trgglu; Tsrglu; NH3; protein-L-glutamate [C]

Alternate protein names: NA

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MIKKIRIAIADDNKEFANILAEYLSAQQNIEVVGVANDGNQAIEIIKNQSPDLLILDIIMPYLDGIGVLEKINEEQLKKP
KILILSAVGQEKITQRAIALGADYYVLKPFDLAMLSKRILELMEPDLDIVSRTVFHVVKAKKDYDLEAMITEIIHEVGVP
AHIKGYMYLRDAISLVISNMEYLNSVTKMLYPKIAEKYNTTPSRVERAIRHAIEVAWSRGRTEVLNELFGYTINDEKGKP
TNSEFIALIADKLRLGMKAG

Sequences:

>Translated_260_residues
MIKKIRIAIADDNKEFANILAEYLSAQQNIEVVGVANDGNQAIEIIKNQSPDLLILDIIMPYLDGIGVLEKINEEQLKKP
KILILSAVGQEKITQRAIALGADYYVLKPFDLAMLSKRILELMEPDLDIVSRTVFHVVKAKKDYDLEAMITEIIHEVGVP
AHIKGYMYLRDAISLVISNMEYLNSVTKMLYPKIAEKYNTTPSRVERAIRHAIEVAWSRGRTEVLNELFGYTINDEKGKP
TNSEFIALIADKLRLGMKAG
>Mature_260_residues
MIKKIRIAIADDNKEFANILAEYLSAQQNIEVVGVANDGNQAIEIIKNQSPDLLILDIIMPYLDGIGVLEKINEEQLKKP
KILILSAVGQEKITQRAIALGADYYVLKPFDLAMLSKRILELMEPDLDIVSRTVFHVVKAKKDYDLEAMITEIIHEVGVP
AHIKGYMYLRDAISLVISNMEYLNSVTKMLYPKIAEKYNTTPSRVERAIRHAIEVAWSRGRTEVLNELFGYTINDEKGKP
TNSEFIALIADKLRLGMKAG

Specific function: May play the central regulatory role in sporulation. It may be an element of the effector pathway responsible for the activation of sporulation genes in response to nutritional stress. Spo0A may act in concert with spo0H (a sigma factor) to control the ex

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788192, Length=109, Percent_Identity=34.8623853211009, Blast_Score=64, Evalue=7e-12,
Organism=Escherichia coli, GI1788550, Length=120, Percent_Identity=32.5, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR016032
- InterPro:   IPR001789
- InterPro:   IPR014879
- InterPro:   IPR012052
- InterPro:   IPR011991
- ProDom:   PD010908 [H]

Pfam domain/function: PF00072 Response_reg; PF08769 Spo0A_C [H]

EC number: 3.1.1.61 [C]

Molecular weight: Translated: 29301; Mature: 29301

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKKIRIAIADDNKEFANILAEYLSAQQNIEVVGVANDGNQAIEIIKNQSPDLLILDIIM
CCCEEEEEEECCCHHHHHHHHHHHHHCCCEEEEEEECCCHHHHHHHHCCCCCEEHHHHHH
PYLDGIGVLEKINEEQLKKPKILILSAVGQEKITQRAIALGADYYVLKPFDLAMLSKRIL
HHHCCCHHHHHCCHHHHCCCCEEEEECCCHHHHHHHHHHHCCCEEEECCHHHHHHHHHHH
ELMEPDLDIVSRTVFHVVKAKKDYDLEAMITEIIHEVGVPAHIKGYMYLRDAISLVISNM
HHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
EYLNSVTKMLYPKIAEKYNTTPSRVERAIRHAIEVAWSRGRTEVLNELFGYTINDEKGKP
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHCEEECCCCCCC
TNSEFIALIADKLRLGMKAG
CCHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MIKKIRIAIADDNKEFANILAEYLSAQQNIEVVGVANDGNQAIEIIKNQSPDLLILDIIM
CCCEEEEEEECCCHHHHHHHHHHHHHCCCEEEEEEECCCHHHHHHHHCCCCCEEHHHHHH
PYLDGIGVLEKINEEQLKKPKILILSAVGQEKITQRAIALGADYYVLKPFDLAMLSKRIL
HHHCCCHHHHHCCHHHHCCCCEEEEECCCHHHHHHHHHHHCCCEEEECCHHHHHHHHHHH
ELMEPDLDIVSRTVFHVVKAKKDYDLEAMITEIIHEVGVPAHIKGYMYLRDAISLVISNM
HHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
EYLNSVTKMLYPKIAEKYNTTPSRVERAIRHAIEVAWSRGRTEVLNELFGYTINDEKGKP
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHCEEECCCCCCC
TNSEFIALIADKLRLGMKAG
CCHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Protein L-glutamate Omethyl ester; Tapglu-Me; H2O; Targlu-Me; Trgglu-Me; Tsrglu-Me; Tsrgln; Trggln; Targln; Tapgln; protein-L-glutamine [C]

Specific reaction: Protein L-glutamate Omethyl ester + H2O = protein L-glutamate+ methanol H2O + Tapglu-Me = methanol + Tapglu H2O + Targlu-Me = methanol + Targlu H2O + Trgglu-Me = methanol + Trgglu H2O + Tsrglu-Me = methanol + Tsrglu H2O + Tsrgln = NH3 + Tsrglu H2O + Trggl

General reaction: Hydrolysis of carboxylic ester [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7885226 [H]