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The map label for this gene is spoIVB [H]

Identifier: 20807752

GI number: 20807752

Start: 1294213

End: 1295499

Strand: Direct

Name: spoIVB [H]

Synonym: TTE1303

Alternate gene names: 20807752

Gene position: 1294213-1295499 (Clockwise)

Preceding gene: 20807751

Following gene: 20807753

Centisome position: 48.12

GC content: 36.21

Gene sequence:

>1287_bases
ATGAATAAGAAATTAATAAAGCTGGTTTTATTCGCCCTATTGAGCTTTTTTTTAGTATTTGCAAACTACTTTACACCTAT
TCAGGAACTAGCAAAAACACCCTCTAATTTCAAAATTTTTCAGGGTGATAAACTAAGTTACAATTTTAAATTTCCTTTTA
AAATTACGTTTGAAATTGACAAAGAAGGGCTTTTGAGAATAAATGGAAAAGATGAGATAACCATTGAAGCAGTAAACACT
GGAAAAGCAAATTTAGATGTAAAATTATTTGGATTTTTACCTTTAAAACACATAAATGTTGATGTAGTGCCTACGATAAA
AGTCGTACCTGGAGGGCAAGCAATAGGAGTGAAACTTAATACAAAAGGAGCCCTAGTGGTAGGTTATTCGGACATAATGG
GGGAAGATGGGAAAATTTACAGCCCGTATAAAGAAGGTAAAATTCAGATTGGAGACATAATTTTAGAAGTAAACGGAGTC
GAGATAAAGCAGGCGGAAGATATTACAAATATTGCTAATAAACTTCAAGGCAAGGCTTTGAAGCTCAAAATTAACAGGAA
AAATGCCATACTTTACACTGTCATTCATCCTGTAAAATCTAAAGAAGATAAGCAGTATAGAATTGGATTGTGGGTGAGGG
ACCATACTGCGGGGATTGGCACTTTGACATTTTATTATCCTTCGCAAAAGGTGTACGCTGCATTAGGGCATGCTATAACT
GACATTGATACGGGGCATGTGCTGTCAGTGGAAAACGGAGAAATAATGAATACTCGCATTACATCTATTGACCAAGGTCG
CAGAAGCAAGCCGGGAGAATTGAAGGGGGTGTTTTTTGAAGAGGTAGATGCGATAGGAAATATTTTAAAAAATACTCAAT
TTGGCATATATGGCTACATGTACAATGACTTTAAGAATGAATTCTATGGAGAGATTCCTGTCGCTTATCAAGGGCAGATA
AAAGAAGGACCTGCTAAAATACTTGCTACCATTGATAATAAAGGAGTACAGGAGTTTGACGTAGAAATTGTGAAAAAGGT
GTATCAAGAGTCACCTACTCCAAAAGGCATGATAGTAAGGATTGTAGATAAGAACCTCTTGGCCAAGACTGGAGGAATAA
TACAGGGTATGAGCGGAAGCCCCATAATTCAAAATGGAAAACTTGTAGGAGCTATAACTCACGTGTTTGTCAATGACCCT
TCAAAAGGCTATGCTGTTTACGCAGAATGGATGATAAATGAAGTGAACAACTTGCTCAACGATAAGCAGGTTTTTATAAA
TGAATAA

Upstream 100 bases:

>100_bases
CCTTTTGCTATCGGGATAATTTATTTACAGTATATAAGCACTTCAAAAGGGTTAATTTAAATGTAGATTCAGCTGAAGTT
TTGAAAAAGGAAGTGAGATG

Downstream 100 bases:

>100_bases
AAATTTTTACCAAAAAGGAGGAAATTCAACTATTATGTAGAATAAATATTATATACTAATATTAGCAACGGGAGGTAAGA
GTAATTGATTAAGAAGATTA

Product: sporulation protein IVB

Products: NA

Alternate protein names: Sporulation factor IV B protease; Stage IV sporulation protein B [H]

Number of amino acids: Translated: 428; Mature: 428

Protein sequence:

>428_residues
MNKKLIKLVLFALLSFFLVFANYFTPIQELAKTPSNFKIFQGDKLSYNFKFPFKITFEIDKEGLLRINGKDEITIEAVNT
GKANLDVKLFGFLPLKHINVDVVPTIKVVPGGQAIGVKLNTKGALVVGYSDIMGEDGKIYSPYKEGKIQIGDIILEVNGV
EIKQAEDITNIANKLQGKALKLKINRKNAILYTVIHPVKSKEDKQYRIGLWVRDHTAGIGTLTFYYPSQKVYAALGHAIT
DIDTGHVLSVENGEIMNTRITSIDQGRRSKPGELKGVFFEEVDAIGNILKNTQFGIYGYMYNDFKNEFYGEIPVAYQGQI
KEGPAKILATIDNKGVQEFDVEIVKKVYQESPTPKGMIVRIVDKNLLAKTGGIIQGMSGSPIIQNGKLVGAITHVFVNDP
SKGYAVYAEWMINEVNNLLNDKQVFINE

Sequences:

>Translated_428_residues
MNKKLIKLVLFALLSFFLVFANYFTPIQELAKTPSNFKIFQGDKLSYNFKFPFKITFEIDKEGLLRINGKDEITIEAVNT
GKANLDVKLFGFLPLKHINVDVVPTIKVVPGGQAIGVKLNTKGALVVGYSDIMGEDGKIYSPYKEGKIQIGDIILEVNGV
EIKQAEDITNIANKLQGKALKLKINRKNAILYTVIHPVKSKEDKQYRIGLWVRDHTAGIGTLTFYYPSQKVYAALGHAIT
DIDTGHVLSVENGEIMNTRITSIDQGRRSKPGELKGVFFEEVDAIGNILKNTQFGIYGYMYNDFKNEFYGEIPVAYQGQI
KEGPAKILATIDNKGVQEFDVEIVKKVYQESPTPKGMIVRIVDKNLLAKTGGIIQGMSGSPIIQNGKLVGAITHVFVNDP
SKGYAVYAEWMINEVNNLLNDKQVFINE
>Mature_428_residues
MNKKLIKLVLFALLSFFLVFANYFTPIQELAKTPSNFKIFQGDKLSYNFKFPFKITFEIDKEGLLRINGKDEITIEAVNT
GKANLDVKLFGFLPLKHINVDVVPTIKVVPGGQAIGVKLNTKGALVVGYSDIMGEDGKIYSPYKEGKIQIGDIILEVNGV
EIKQAEDITNIANKLQGKALKLKINRKNAILYTVIHPVKSKEDKQYRIGLWVRDHTAGIGTLTFYYPSQKVYAALGHAIT
DIDTGHVLSVENGEIMNTRITSIDQGRRSKPGELKGVFFEEVDAIGNILKNTQFGIYGYMYNDFKNEFYGEIPVAYQGQI
KEGPAKILATIDNKGVQEFDVEIVKKVYQESPTPKGMIVRIVDKNLLAKTGGIIQGMSGSPIIQNGKLVGAITHVFVNDP
SKGYAVYAEWMINEVNNLLNDKQVFINE

Specific function: Plays a central role in the sigma-K checkpoint which coordinates gene expression during the later stages of spore formation. The protease is activated by trans cleavage of the zymogen precursor producing SpoIVB-45 kDa. This undergoes further trimming by c

COG id: COG0750

COG function: function code M; Predicted membrane-associated Zn-dependent proteases 1

Gene ontology:

Cell location: Forespore intermembrane space [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 peptidase S55 domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001478
- InterPro:   IPR014219
- InterPro:   IPR008763 [H]

Pfam domain/function: PF00595 PDZ; PF05580 Peptidase_S55 [H]

EC number: =3.4.21.116 [H]

Molecular weight: Translated: 47664; Mature: 47664

Theoretical pI: Translated: 9.45; Mature: 9.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKKLIKLVLFALLSFFLVFANYFTPIQELAKTPSNFKIFQGDKLSYNFKFPFKITFEID
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCEEEEEEECCEEEEEEEC
KEGLLRINGKDEITIEAVNTGKANLDVKLFGFLPLKHINVDVVPTIKVVPGGQAIGVKLN
CCCEEEECCCCEEEEEEEECCCCEEEEEEEEEEEEEECCEEEEEEEEEECCCCEEEEEEC
TKGALVVGYSDIMGEDGKIYSPYKEGKIQIGDIILEVNGVEIKQAEDITNIANKLQGKAL
CCCEEEEEEHHHCCCCCCEECCCCCCCEEEEEEEEEECCEEEECHHHHHHHHHHHCCCEE
KLKINRKNAILYTVIHPVKSKEDKQYRIGLWVRDHTAGIGTLTFYYPSQKVYAALGHAIT
EEEEECCCEEEEEEEECCCCCCCCEEEEEEEEEECCCCEEEEEEEECCHHHHHHHHHHHC
DIDTGHVLSVENGEIMNTRITSIDQGRRSKPGELKGVFFEEVDAIGNILKNTQFGIYGYM
CCCCCCEEEECCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEE
YNDFKNEFYGEIPVAYQGQIKEGPAKILATIDNKGVQEFDVEIVKKVYQESPTPKGMIVR
EHHHCHHHCCCCCEEEECCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCEEEE
IVDKNLLAKTGGIIQGMSGSPIIQNGKLVGAITHVFVNDPSKGYAVYAEWMINEVNNLLN
EECCCHHHHCCCEEECCCCCCEEECCEEEEEEEEEEECCCCCCEEEEHHHHHHHHHHHCC
DKQVFINE
CCEEEEEC
>Mature Secondary Structure
MNKKLIKLVLFALLSFFLVFANYFTPIQELAKTPSNFKIFQGDKLSYNFKFPFKITFEID
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCEEEEEEECCEEEEEEEC
KEGLLRINGKDEITIEAVNTGKANLDVKLFGFLPLKHINVDVVPTIKVVPGGQAIGVKLN
CCCEEEECCCCEEEEEEEECCCCEEEEEEEEEEEEEECCEEEEEEEEEECCCCEEEEEEC
TKGALVVGYSDIMGEDGKIYSPYKEGKIQIGDIILEVNGVEIKQAEDITNIANKLQGKAL
CCCEEEEEEHHHCCCCCCEECCCCCCCEEEEEEEEEECCEEEECHHHHHHHHHHHCCCEE
KLKINRKNAILYTVIHPVKSKEDKQYRIGLWVRDHTAGIGTLTFYYPSQKVYAALGHAIT
EEEEECCCEEEEEEEECCCCCCCCEEEEEEEEEECCCCEEEEEEEECCHHHHHHHHHHHC
DIDTGHVLSVENGEIMNTRITSIDQGRRSKPGELKGVFFEEVDAIGNILKNTQFGIYGYM
CCCCCCEEEECCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEE
YNDFKNEFYGEIPVAYQGQIKEGPAKILATIDNKGVQEFDVEIVKKVYQESPTPKGMIVR
EHHHCHHHCCCCCEEEECCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCEEEE
IVDKNLLAKTGGIIQGMSGSPIIQNGKLVGAITHVFVNDPSKGYAVYAEWMINEVNNLLN
EECCCHHHHCCCEEECCCCCCEEECCEEEEEEEEEEECCCCCCEEEEHHHHHHHHHHHCC
DKQVFINE
CCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2106508; 8969508; 9384377; 11741860 [H]