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The map label for this gene is SpoT [H]

Identifier: 20807650

GI number: 20807650

Start: 1193873

End: 1196029

Strand: Direct

Name: SpoT [H]

Synonym: TTE1195

Alternate gene names: 20807650

Gene position: 1193873-1196029 (Clockwise)

Preceding gene: 20807649

Following gene: 20807651

Centisome position: 44.39

GC content: 39.64

Gene sequence:

>2157_bases
GTGATAGAAAAAGTAATAAATAGAGTAAAACAATACATGGGGGAAAATGCAAATTTAGACCTTTTATATAAAGCCTACAA
CTTTGCAGTGAAAGCCCATGAGGGGCAGGTAAGGAATTCAGGGGAGCCTTACATTGTGCATCCTGTAGAAGTCGCATACA
TTCTTGCTGATTTGGAGCTAGACCTTACTACAATTGCTGCAGGGCTTTTGCACGACGTGATTGAGGACACAGGTGTTACT
TATGACCAGTTGATGGAAGAATTTGGAAAAGAAATTGCTGACCTGGTAGATGGGGTTACCAAATTGGGAAAGATAGAGTA
TAAAAGCAAAGTTGAGCAACAGGCTGAAAACATGAGAAAAATGTTAATAGCGATGGCAAAGGATATAAGGGTGATACTCA
TAAAATTAGCCGACAGGCTTCACAACATGAGAACTTTAAAGTATTTGCCTCCTGACAAGCAGAAGGAAAAGGCAGAGGAG
ACATTAGAAATTTATGCGCCTATAGCCCACAGACTGGGAATATCAAAAATAAAATGGGAATTGGAAGATCTGTGCCTGAG
GTATTTGCACCCTGAAGAGTATTACGACTTAGTAGAAAAAGTTGCTGCAAAGAGAAAAGAGAGAGAAGAATTTATACAAA
ACATAATAGCCACTATCAAGCAAAAATTAGATGAGATGGGTATACCAGCGGAAGTAGATGGGCGGCCAAAGCATTTTTAC
AGCATATACAAAAAAATGAAGACTCAAAATAAGACTTTTGAAGAAATTTACGACCTTTTAGCTATAAGAATAATTGTAAA
TACGGTAAAAGACTGCTACGGCGTGTTGGGAATAGTGCATACCTTATGGAAGCCAATTCCAGGACGTTTTAAAGATTACA
TTGCTATGCCTAAGCCAAATATGTATCAATCTCTTCATACCACAGTTATAGGCCCCAAAGGAGAGCCTTTTGAAGTACAG
ATAAGGACGTGGGAGATGCATAAAACTGCTGAATACGGTATTGCAGCCCACTGGCGCTATAAAGAAGGGAAGACCACAGA
AGACGAGTTTGACAAAAAGCTTGTATGGTTAAGGCAGCTTTTAGAATGGCAGAAGGAATTGAAAGACCCTAAAGAGTTTA
TGGAAACTTTAAAGATAGACCTTTTTACAGACGAAGTTTTTGTGTTTACTCCAAAAGGGGATGTCATCAGCTTGCCGGCA
GGGTCTACTCCTATCGATTTTGCGTACAGCATACACACTGAGATAGGTCACAGGATGAATGGTGCCAAAGTAAATGGGAG
AATAGTTCCTATAAACTATCAGCTTAAGACGGGAGATATTGTGGAAATACTAGTAAGTCCTAATAAAGATAGAGGGCCTA
GTAGGGATTGGCTGCAGATTGTCAAGACTTCTCAGGCGAGAAACAAAATAAGGCAGTGGTTTAAAAAAGAGAAAAGGGAA
GAGAATATAGCTCGCGGAGAAGAAATGTTAGAGAAAGAGCTGCGGCGCCAGGGGATACAGCCTTCAATGATTAAGACAGA
AATATGGGAGGAAGTGTTAAAGAAATTAAACATCCATACGATGGAGGACTTATATGCTACAATAGGTTATGGCGGACTTA
CGTTAAACCAGGTAATTCCAAGAATTAAGGAAGAAATAAGAAAATCTCAAAAAACCGTTCCTAAAGTCCAGACTCCCACA
AGAGAAGAGAAGAAGAAAGAGAAGAAAAATGGCGCTGGAGTCATTGTAAAGGGCGTTGACAATGTCATGGTCAGATTTGC
TAAGTGCTGTTCTCCTGTGCCGGGAGATGAAATAGTTGGATATGTGACGAGAGGAAGAGGAATTTCCATTCACAGGAAAG
ATTGTCCGAATGTGAGAGACCTCCTGTATGACAAAAACAGAATAGTCGAAGTGGAATGGGCACAGGACAGAAACATGGCC
TATCAAACAGATATTCAGATTATGGCAAATGATAAGTTTGGGCTTTTGACAGAAGTTACTGGAGTTTTGGCAGATGCTAA
AATAAATGTAAAAGCTATAAACGCCAGGACTACTAGAGACAACATAGCCATTATAAATCTCACTTTAGAGATTACTTCAA
AAGAGCAGTTAGAAAAGGTGATGAACCGCCTTAAGTCTCTAGAAGGAGTAATGGATGTGTACAGATTAAGTGCATAG

Upstream 100 bases:

>100_bases
GTAGAAAAAAGCTTGACGGATATGATATAATTTCATTAATAAAGTTCTAAATATCTTTAAAGTGGCATTTTGCCACTTTA
AATTATAAGGTGTGATGACT

Downstream 100 bases:

>100_bases
GGGTGATTTTGTGAGAGCTGTTGTACAAAGAGTAACTCGCGGAGAAGTGAGGGTAGACGGCGAAGTTGTGGGGGCTATAG
GAAAAGGGTTTGTGGTTTTA

Product: guanosine polyphosphate pyrophosphohydrolase/synthetase

Products: NA

Alternate protein names: (p)ppGpp synthase; ATP:GTP 3'-pyrophosphotransferase; ppGpp synthase I [H]

Number of amino acids: Translated: 718; Mature: 718

Protein sequence:

>718_residues
MIEKVINRVKQYMGENANLDLLYKAYNFAVKAHEGQVRNSGEPYIVHPVEVAYILADLELDLTTIAAGLLHDVIEDTGVT
YDQLMEEFGKEIADLVDGVTKLGKIEYKSKVEQQAENMRKMLIAMAKDIRVILIKLADRLHNMRTLKYLPPDKQKEKAEE
TLEIYAPIAHRLGISKIKWELEDLCLRYLHPEEYYDLVEKVAAKRKEREEFIQNIIATIKQKLDEMGIPAEVDGRPKHFY
SIYKKMKTQNKTFEEIYDLLAIRIIVNTVKDCYGVLGIVHTLWKPIPGRFKDYIAMPKPNMYQSLHTTVIGPKGEPFEVQ
IRTWEMHKTAEYGIAAHWRYKEGKTTEDEFDKKLVWLRQLLEWQKELKDPKEFMETLKIDLFTDEVFVFTPKGDVISLPA
GSTPIDFAYSIHTEIGHRMNGAKVNGRIVPINYQLKTGDIVEILVSPNKDRGPSRDWLQIVKTSQARNKIRQWFKKEKRE
ENIARGEEMLEKELRRQGIQPSMIKTEIWEEVLKKLNIHTMEDLYATIGYGGLTLNQVIPRIKEEIRKSQKTVPKVQTPT
REEKKKEKKNGAGVIVKGVDNVMVRFAKCCSPVPGDEIVGYVTRGRGISIHRKDCPNVRDLLYDKNRIVEVEWAQDRNMA
YQTDIQIMANDKFGLLTEVTGVLADAKINVKAINARTTRDNIAIINLTLEITSKEQLEKVMNRLKSLEGVMDVYRLSA

Sequences:

>Translated_718_residues
MIEKVINRVKQYMGENANLDLLYKAYNFAVKAHEGQVRNSGEPYIVHPVEVAYILADLELDLTTIAAGLLHDVIEDTGVT
YDQLMEEFGKEIADLVDGVTKLGKIEYKSKVEQQAENMRKMLIAMAKDIRVILIKLADRLHNMRTLKYLPPDKQKEKAEE
TLEIYAPIAHRLGISKIKWELEDLCLRYLHPEEYYDLVEKVAAKRKEREEFIQNIIATIKQKLDEMGIPAEVDGRPKHFY
SIYKKMKTQNKTFEEIYDLLAIRIIVNTVKDCYGVLGIVHTLWKPIPGRFKDYIAMPKPNMYQSLHTTVIGPKGEPFEVQ
IRTWEMHKTAEYGIAAHWRYKEGKTTEDEFDKKLVWLRQLLEWQKELKDPKEFMETLKIDLFTDEVFVFTPKGDVISLPA
GSTPIDFAYSIHTEIGHRMNGAKVNGRIVPINYQLKTGDIVEILVSPNKDRGPSRDWLQIVKTSQARNKIRQWFKKEKRE
ENIARGEEMLEKELRRQGIQPSMIKTEIWEEVLKKLNIHTMEDLYATIGYGGLTLNQVIPRIKEEIRKSQKTVPKVQTPT
REEKKKEKKNGAGVIVKGVDNVMVRFAKCCSPVPGDEIVGYVTRGRGISIHRKDCPNVRDLLYDKNRIVEVEWAQDRNMA
YQTDIQIMANDKFGLLTEVTGVLADAKINVKAINARTTRDNIAIINLTLEITSKEQLEKVMNRLKSLEGVMDVYRLSA
>Mature_718_residues
MIEKVINRVKQYMGENANLDLLYKAYNFAVKAHEGQVRNSGEPYIVHPVEVAYILADLELDLTTIAAGLLHDVIEDTGVT
YDQLMEEFGKEIADLVDGVTKLGKIEYKSKVEQQAENMRKMLIAMAKDIRVILIKLADRLHNMRTLKYLPPDKQKEKAEE
TLEIYAPIAHRLGISKIKWELEDLCLRYLHPEEYYDLVEKVAAKRKEREEFIQNIIATIKQKLDEMGIPAEVDGRPKHFY
SIYKKMKTQNKTFEEIYDLLAIRIIVNTVKDCYGVLGIVHTLWKPIPGRFKDYIAMPKPNMYQSLHTTVIGPKGEPFEVQ
IRTWEMHKTAEYGIAAHWRYKEGKTTEDEFDKKLVWLRQLLEWQKELKDPKEFMETLKIDLFTDEVFVFTPKGDVISLPA
GSTPIDFAYSIHTEIGHRMNGAKVNGRIVPINYQLKTGDIVEILVSPNKDRGPSRDWLQIVKTSQARNKIRQWFKKEKRE
ENIARGEEMLEKELRRQGIQPSMIKTEIWEEVLKKLNIHTMEDLYATIGYGGLTLNQVIPRIKEEIRKSQKTVPKVQTPT
REEKKKEKKNGAGVIVKGVDNVMVRFAKCCSPVPGDEIVGYVTRGRGISIHRKDCPNVRDLLYDKNRIVEVEWAQDRNMA
YQTDIQIMANDKFGLLTEVTGVLADAKINVKAINARTTRDNIAIINLTLEITSKEQLEKVMNRLKSLEGVMDVYRLSA

Specific function: In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the formation of pppGpp which i

COG id: COG0317

COG function: function code TK; Guanosine polyphosphate pyrophosphohydrolases/synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HD domain [H]

Homologues:

Organism=Homo sapiens, GI38348360, Length=139, Percent_Identity=38.1294964028777, Blast_Score=71, Evalue=3e-12,
Organism=Escherichia coli, GI1790082, Length=716, Percent_Identity=42.4581005586592, Blast_Score=590, Evalue=1e-169,
Organism=Escherichia coli, GI1789147, Length=689, Percent_Identity=41.3642960812772, Blast_Score=581, Evalue=1e-167,
Organism=Caenorhabditis elegans, GI25143118, Length=140, Percent_Identity=35.7142857142857, Blast_Score=78, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR012675
- InterPro:   IPR003607
- InterPro:   IPR007685
- InterPro:   IPR004811
- InterPro:   IPR004095
- InterPro:   IPR012676 [H]

Pfam domain/function: PF01842 ACT; PF04607 RelA_SpoT; PF02824 TGS [H]

EC number: =2.7.6.5 [H]

Molecular weight: Translated: 82903; Mature: 82903

Theoretical pI: Translated: 8.93; Mature: 8.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEKVINRVKQYMGENANLDLLYKAYNFAVKAHEGQVRNSGEPYIVHPVEVAYILADLEL
CHHHHHHHHHHHHCCCCCEEEEEEEHHHEEEECCCCCCCCCCCEEEEHHHEEHHHHHCCH
DLTTIAAGLLHDVIEDTGVTYDQLMEEFGKEIADLVDGVTKLGKIEYKSKVEQQAENMRK
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
MLIAMAKDIRVILIKLADRLHNMRTLKYLPPDKQKEKAEETLEIYAPIAHRLGISKIKWE
HHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHH
LEDLCLRYLHPEEYYDLVEKVAAKRKEREEFIQNIIATIKQKLDEMGIPAEVDGRPKHFY
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHH
SIYKKMKTQNKTFEEIYDLLAIRIIVNTVKDCYGVLGIVHTLWKPIPGRFKDYIAMPKPN
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCH
MYQSLHTTVIGPKGEPFEVQIRTWEMHKTAEYGIAAHWRYKEGKTTEDEFDKKLVWLRQL
HHHHHHHEEECCCCCCEEEEEEEEEEHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHH
LEWQKELKDPKEFMETLKIDLFTDEVFVFTPKGDVISLPAGSTPIDFAYSIHTEIGHRMN
HHHHHHHCCHHHHHHHHHHEEEECCEEEECCCCCEEECCCCCCCCCHHEEEHHHHCCCCC
GAKVNGRIVPINYQLKTGDIVEILVSPNKDRGPSRDWLQIVKTSQARNKIRQWFKKEKRE
CCEECCEEEEEEEEECCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
ENIARGEEMLEKELRRQGIQPSMIKTEIWEEVLKKLNIHTMEDLYATIGYGGLTLNQVIP
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHH
RIKEEIRKSQKTVPKVQTPTREEKKKEKKNGAGVIVKGVDNVMVRFAKCCSPVPGDEIVG
HHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCEEEEECHHHHHHHHHHHCCCCCCCHHEE
YVTRGRGISIHRKDCPNVRDLLYDKNRIVEVEWAQDRNMAYQTDIQIMANDKFGLLTEVT
EEECCCCCEEECCCCCCHHHHHCCCCCEEEEEECCCCCCEEEECEEEEECCCCCHHHHHH
GVLADAKINVKAINARTTRDNIAIINLTLEITSKEQLEKVMNRLKSLEGVMDVYRLSA
HHHHCCEEEEEEEECCCCCCCEEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIEKVINRVKQYMGENANLDLLYKAYNFAVKAHEGQVRNSGEPYIVHPVEVAYILADLEL
CHHHHHHHHHHHHCCCCCEEEEEEEHHHEEEECCCCCCCCCCCEEEEHHHEEHHHHHCCH
DLTTIAAGLLHDVIEDTGVTYDQLMEEFGKEIADLVDGVTKLGKIEYKSKVEQQAENMRK
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
MLIAMAKDIRVILIKLADRLHNMRTLKYLPPDKQKEKAEETLEIYAPIAHRLGISKIKWE
HHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHH
LEDLCLRYLHPEEYYDLVEKVAAKRKEREEFIQNIIATIKQKLDEMGIPAEVDGRPKHFY
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHH
SIYKKMKTQNKTFEEIYDLLAIRIIVNTVKDCYGVLGIVHTLWKPIPGRFKDYIAMPKPN
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCH
MYQSLHTTVIGPKGEPFEVQIRTWEMHKTAEYGIAAHWRYKEGKTTEDEFDKKLVWLRQL
HHHHHHHEEECCCCCCEEEEEEEEEEHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHH
LEWQKELKDPKEFMETLKIDLFTDEVFVFTPKGDVISLPAGSTPIDFAYSIHTEIGHRMN
HHHHHHHCCHHHHHHHHHHEEEECCEEEECCCCCEEECCCCCCCCCHHEEEHHHHCCCCC
GAKVNGRIVPINYQLKTGDIVEILVSPNKDRGPSRDWLQIVKTSQARNKIRQWFKKEKRE
CCEECCEEEEEEEEECCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
ENIARGEEMLEKELRRQGIQPSMIKTEIWEEVLKKLNIHTMEDLYATIGYGGLTLNQVIP
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHH
RIKEEIRKSQKTVPKVQTPTREEKKKEKKNGAGVIVKGVDNVMVRFAKCCSPVPGDEIVG
HHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCEEEEECHHHHHHHHHHHCCCCCCCHHEE
YVTRGRGISIHRKDCPNVRDLLYDKNRIVEVEWAQDRNMAYQTDIQIMANDKFGLLTEVT
EEECCCCCEEECCCCCCHHHHHCCCCCEEEEEECCCCCCEEEECEEEEECCCCCHHHHHH
GVLADAKINVKAINARTTRDNIAIINLTLEITSKEQLEKVMNRLKSLEGVMDVYRLSA
HHHHCCEEEEEEEECCCCCCCEEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9383190; 9384377 [H]