Click here to switch to the map view.

The map label for this gene is AroA2

Identifier: 20807495

GI number: 20807495

Start: 1017274

End: 1018545

Strand: Direct

Name: AroA2

Synonym: TTE1015

Alternate gene names: 20807495

Gene position: 1017274-1018545 (Clockwise)

Preceding gene: 20807494

Following gene: 20807496

Centisome position: 37.82

GC content: 38.21

Gene sequence:

>1272_bases
ATGGATGTAGAGGTAAAAAAGAAAAACAGCTTAAAAGGTGTTATTTCTGTCCCGGGAGACAAATCTATATCTCACAGAGC
TGTTATGATAGGTTCAATAGCTGAAGGGCTAACAGAAGTAGAAAATTTTCTTGTTGCTAAGGATACTCTTTCTACTGTAG
AGTGTATGAGAAAACTGGGTGTAGACATTAAATTGGAAAGAAATAGAGTGAAAGTGTATGGAAAAGGATTGTACCTTAGT
AAGAGCAGTGAAGTGTTAGATGTAGGAAATTCTGGGACAACAATTAGGCTTTTGATGGGAATACTTGCAGGGCAAGAGTT
TGAGACAATACTCACAGGGGATGATTCTATCAAGAGGCGGCCTATGGGAAGAGTGATAGTGCCTCTTACTCTCATGGGGG
CTAAAATAGAGGCAAGGGAAGGGAATTTTGCTCCTGTCAAAATAAGAGGTGGAAAACTTCGAGGAATAGAGTATAAAATG
CCTATTGCCAGTGCACAGGTTAAATCTAGCATAATGCTTGCAAGCCTTTATGCAGAAGGAAGGACAGTGATTGAAGAGCC
TGCTTTATCGAGAAATCATACGGAGCTTATGCTTCAAACTTTTGGTGCAAGGATAGAGATAGATGGAAAGAAAATATTTT
GTTATCCGGGAAGTAAATTAAAAGGGCAGAAAATTATAGTGCCTGGAGATATATCTTCTGCTGCCTATTTTATAGTGGCA
GCAAATTTGCTTCCTAATTCAGAGGTGGTTATAAAAGATGTGAATGTAAATCCTACGAGAACAGGAATTATAGATGTTTT
AAAAGGTATGGGAGGAAATGTTGAGCTTATAAATGAAAGATATGTGAATAATGAAAAAATAGCGGACATTGTAGTTAAAA
GTTCTAAATTAAAGGGTATAGAAATCGGCGGAGACCTTATTCCTAAGCTTATTGATGAAATTCCTGTTATCGCAGTTGCA
GCAGTTTTTGCTGAAGGAACCACTGTGATAAAAAATGCAGAAGAATTGAAGGTAAAAGAGAGCAACAGAATAAAATCAAT
GACATCAGAGCTTGGGAAAATGGGAGCTAAAATTTTTGAGACAGAAGATGGAATGATTATAGAAGGGACAGGCTTTTTAA
GAGGAGCCGAAGTTGAGAGTTACAACGACCACCGTGTGGCCATGTCCTTGTGGATTGCTGGCCTTTTAGCAGAAGGTCGG
ACCATAATTAAGAAGGCTGAATGCGTCGACGTTTCATATCCCGATTTCTATAAGACATTTGATATGCTATAA

Upstream 100 bases:

>100_bases
ATTTGTGTATCATCTGGAGAGAGGAGATGAAGAGTTTTTAGTGGACTTCCTTGACAAGGCCCGAGAATGCAGAAAAATAC
TAGAGGTATGAGGTGAGAAA

Downstream 100 bases:

>100_bases
TTTTATTGGTGATGGCCAATGAAGGAAAAGATACTGATTGTAGAAGATGAAAGACAGATAGCGCGTTTTTTACAATTGGA
ATTCGAACACGAAGGCTATG

Product: 3-phosphoshikimate 1-carboxyvinyltransferase

Products: NA

Alternate protein names: 5-enolpyruvylshikimate-3-phosphate synthase; EPSP synthase; EPSPS

Number of amino acids: Translated: 423; Mature: 423

Protein sequence:

>423_residues
MDVEVKKKNSLKGVISVPGDKSISHRAVMIGSIAEGLTEVENFLVAKDTLSTVECMRKLGVDIKLERNRVKVYGKGLYLS
KSSEVLDVGNSGTTIRLLMGILAGQEFETILTGDDSIKRRPMGRVIVPLTLMGAKIEAREGNFAPVKIRGGKLRGIEYKM
PIASAQVKSSIMLASLYAEGRTVIEEPALSRNHTELMLQTFGARIEIDGKKIFCYPGSKLKGQKIIVPGDISSAAYFIVA
ANLLPNSEVVIKDVNVNPTRTGIIDVLKGMGGNVELINERYVNNEKIADIVVKSSKLKGIEIGGDLIPKLIDEIPVIAVA
AVFAEGTTVIKNAEELKVKESNRIKSMTSELGKMGAKIFETEDGMIIEGTGFLRGAEVESYNDHRVAMSLWIAGLLAEGR
TIIKKAECVDVSYPDFYKTFDML

Sequences:

>Translated_423_residues
MDVEVKKKNSLKGVISVPGDKSISHRAVMIGSIAEGLTEVENFLVAKDTLSTVECMRKLGVDIKLERNRVKVYGKGLYLS
KSSEVLDVGNSGTTIRLLMGILAGQEFETILTGDDSIKRRPMGRVIVPLTLMGAKIEAREGNFAPVKIRGGKLRGIEYKM
PIASAQVKSSIMLASLYAEGRTVIEEPALSRNHTELMLQTFGARIEIDGKKIFCYPGSKLKGQKIIVPGDISSAAYFIVA
ANLLPNSEVVIKDVNVNPTRTGIIDVLKGMGGNVELINERYVNNEKIADIVVKSSKLKGIEIGGDLIPKLIDEIPVIAVA
AVFAEGTTVIKNAEELKVKESNRIKSMTSELGKMGAKIFETEDGMIIEGTGFLRGAEVESYNDHRVAMSLWIAGLLAEGR
TIIKKAECVDVSYPDFYKTFDML
>Mature_423_residues
MDVEVKKKNSLKGVISVPGDKSISHRAVMIGSIAEGLTEVENFLVAKDTLSTVECMRKLGVDIKLERNRVKVYGKGLYLS
KSSEVLDVGNSGTTIRLLMGILAGQEFETILTGDDSIKRRPMGRVIVPLTLMGAKIEAREGNFAPVKIRGGKLRGIEYKM
PIASAQVKSSIMLASLYAEGRTVIEEPALSRNHTELMLQTFGARIEIDGKKIFCYPGSKLKGQKIIVPGDISSAAYFIVA
ANLLPNSEVVIKDVNVNPTRTGIIDVLKGMGGNVELINERYVNNEKIADIVVKSSKLKGIEIGGDLIPKLIDEIPVIAVA
AVFAEGTTVIKNAEELKVKESNRIKSMTSELGKMGAKIFETEDGMIIEGTGFLRGAEVESYNDHRVAMSLWIAGLLAEGR
TIIKKAECVDVSYPDFYKTFDML

Specific function: Aromatic amino acids biosynthesis; shikimate pathway; sixth step. [C]

COG id: COG0128

COG function: function code E; 5-enolpyruvylshikimate-3-phosphate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the EPSP synthase family

Homologues:

Organism=Escherichia coli, GI1787137, Length=427, Percent_Identity=30.4449648711944, Blast_Score=161, Evalue=9e-41,
Organism=Escherichia coli, GI1789580, Length=418, Percent_Identity=24.8803827751196, Blast_Score=69, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6320332, Length=425, Percent_Identity=28, Blast_Score=112, Evalue=1e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): AROA_THETN (Q8RB11)

Other databases:

- EMBL:   AE008691
- RefSeq:   NP_622666.1
- ProteinModelPortal:   Q8RB11
- SMR:   Q8RB11
- GeneID:   995769
- GenomeReviews:   AE008691_GR
- KEGG:   tte:TTE1015
- NMPDR:   fig|273068.3.peg.480
- HOGENOM:   HBG646626
- OMA:   LSRNHTE
- ProtClustDB:   PRK02427
- BioCyc:   TTEN273068:TTE1015-MONOMER
- BRENDA:   2.5.1.19
- GO:   GO:0005737
- HAMAP:   MF_00210
- InterPro:   IPR001986
- InterPro:   IPR006264
- InterPro:   IPR023193
- InterPro:   IPR013792
- Gene3D:   G3DSA:3.65.10.10
- PIRSF:   PIRSF000505
- TIGRFAMs:   TIGR01356

Pfam domain/function: PF00275 EPSP_synthase; SSF55205 RNA3'_cycl/enolpyr_transf_A/B

EC number: =2.5.1.19

Molecular weight: Translated: 46070; Mature: 46070

Theoretical pI: Translated: 8.93; Mature: 8.93

Prosite motif: PS00104 EPSP_SYNTHASE_1; PS00885 EPSP_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDVEVKKKNSLKGVISVPGDKSISHRAVMIGSIAEGLTEVENFLVAKDTLSTVECMRKLG
CCCEECCCCCCCEEEECCCCCCCCCCEEEEHHHHHHHHHHHHHEEEHHHHHHHHHHHHHC
VDIKLERNRVKVYGKGLYLSKSSEVLDVGNSGTTIRLLMGILAGQEFETILTGDDSIKRR
CEEEEECCEEEEEEEEEEEECCCCEEECCCCCCHHHHHHHHHCCCCEEEEEECCCHHHCC
PMGRVIVPLTLMGAKIEAREGNFAPVKIRGGKLRGIEYKMPIASAQVKSSIMLASLYAEG
CCCCEEEEEEEECCEEEECCCCEEEEEEECCEEECEEEECCCCHHHHHHHHHEEEHHHCC
RTVIEEPALSRNHTELMLQTFGARIEIDGKKIFCYPGSKLKGQKIIVPGDISSAAYFIVA
CEEECCCCCCCCHHHHHHHHCCCEEEECCCEEEEECCCCCCCCEEEECCCCCCCEEEEEE
ANLLPNSEVVIKDVNVNPTRTGIIDVLKGMGGNVELINERYVNNEKIADIVVKSSKLKGI
EECCCCCCEEEEECCCCCCHHHHHHHHHCCCCCEEEEEEECCCCCHHEEEEEECCCCCEE
EIGGDLIPKLIDEIPVIAVAAVFAEGTTVIKNAEELKVKESNRIKSMTSELGKMGAKIFE
EECHHHHHHHHHHCCHHHHHHHHHCCCCEECCHHHHEECHHHHHHHHHHHHHHCCCEEEE
TEDGMIIEGTGFLRGAEVESYNDHRVAMSLWIAGLLAEGRTIIKKAECVDVSYPDFYKTF
CCCCEEEECCCCEECCCCCCCCCCHHHHHHHHHHHHHCCHHHEEEEEEEECCCCHHHHHH
DML
CCC
>Mature Secondary Structure
MDVEVKKKNSLKGVISVPGDKSISHRAVMIGSIAEGLTEVENFLVAKDTLSTVECMRKLG
CCCEECCCCCCCEEEECCCCCCCCCCEEEEHHHHHHHHHHHHHEEEHHHHHHHHHHHHHC
VDIKLERNRVKVYGKGLYLSKSSEVLDVGNSGTTIRLLMGILAGQEFETILTGDDSIKRR
CEEEEECCEEEEEEEEEEEECCCCEEECCCCCCHHHHHHHHHCCCCEEEEEECCCHHHCC
PMGRVIVPLTLMGAKIEAREGNFAPVKIRGGKLRGIEYKMPIASAQVKSSIMLASLYAEG
CCCCEEEEEEEECCEEEECCCCEEEEEEECCEEECEEEECCCCHHHHHHHHHEEEHHHCC
RTVIEEPALSRNHTELMLQTFGARIEIDGKKIFCYPGSKLKGQKIIVPGDISSAAYFIVA
CEEECCCCCCCCHHHHHHHHCCCEEEECCCEEEEECCCCCCCCEEEECCCCCCCEEEEEE
ANLLPNSEVVIKDVNVNPTRTGIIDVLKGMGGNVELINERYVNNEKIADIVVKSSKLKGI
EECCCCCCEEEEECCCCCCHHHHHHHHHCCCCCEEEEEEECCCCCHHEEEEEECCCCCEE
EIGGDLIPKLIDEIPVIAVAAVFAEGTTVIKNAEELKVKESNRIKSMTSELGKMGAKIFE
EECHHHHHHHHHHCCHHHHHHHHHCCCCEECCHHHHEECHHHHHHHHHHHHHHCCCEEEE
TEDGMIIEGTGFLRGAEVESYNDHRVAMSLWIAGLLAEGRTIIKKAECVDVSYPDFYKTF
CCCCEEEECCCCEECCCCCCCCCCHHHHHHHHHHHHHCCHHHEEEEEEEECCCCHHHHHH
DML
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11997336