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The map label for this gene is NemA2 [C]

Identifier: 20807468

GI number: 20807468

Start: 991741

End: 993561

Strand: Direct

Name: NemA2 [C]

Synonym: TTE0988

Alternate gene names: 20807468

Gene position: 991741-993561 (Clockwise)

Preceding gene: 20807465

Following gene: 20807469

Centisome position: 36.88

GC content: 36.46

Gene sequence:

>1821_bases
ATGCTCTTAAAAGAAGGGAAAATAGGCGGGTTACATTTAAAAAATAGAATTGTGATGCTTCCGACCGTTACAAATTTGTC
AAAGGATGGCTACGTCACAGAAAAAGAGATAGAGTACTACAGAAGAAGGTCAAGGTCAGTTGCTTTAGTGATCGTAGGAG
CAAGTTTCGTAAATCCATTAGGCAAGTTCTTTCCCAATCAAATAGGAATAGATGCAGATGATAAAATAGAAGGTCTTAGC
AAATTGGCAGAGGCAATACATCAAAATGGTGCTAAAGCAGCAATTCAACTGGCAATGCACAATCCAAAGTACAAACCAAC
AGATTTTCCAAAAGAAAAAATAAAAAACTTTGTGAAGGATTTTGTCAGAGGAGCGATAAGAGCAAAGAAAGCGGATTTTG
ATGCAGTAGAACTGCACTTTGCCCATGGATGGTTTGTAAGTCAGTTTTTGTCACCAAATACAAATAAAAGAGAAGATGAG
TATGGGGGAAGTTTAGAAGGAAGGGTAAGATTTGCCGTTGAAATCTTAAAAGAGATAAAAGATGCTCTACCAGATTTTAC
GGTGATTTGCAGGATTAATGGAGATGATTTCACGGATGGGGGATTCAATATAAAAGAAAGTATTGAATTTGCCAAAATAC
TGGAGAAAAATGGAGCTTCTGCTATAGATGTATCTTCAGGAGTGTCTTCAACTTCTGAGTATCATATTTCTCCAATGGGG
ATAGAAGATAGACCATTATTACCTTTTACTAAAAAGATAAAGGAAAGTATTAGTATTCCTGTAATTGCTGCTAATAAGTT
AGGGGATGTTTACGACTGGGAAAGGATTTTAGAGGAAAGAATAGCCGATTTTATAGGTATTGCCAGAGGTTTGATTGGAG
ATCCCGATTTGGCTGAGAAATTGATTAAGGGAGAAGAAACAGATATAAGGTATTGCATTCACTGCAATCAAGCTTGTATT
GCTTATATACAGAAAGGGCTTTCGGTTTCTTGCATGATTAATCCAGAGGTAGGAAGAGAAAGAGAGTTTGAGGTAAAAAC
TGATAAACCGCTAAATATAGCAGTAATTGGCGGAGGTCCTGCTGGAATGTCTGCAGCTAAATATCTGGCAAGAAAAGGCC
ACAATGTTACACTTTTTGAAAAAGAGAATAGGTTAGGAGGACAGTTAAATGTTGCTCAAATTCCTCCTCACAAGCAAGAG
ATTGGGAGAGTTATTGAATATTTGAAAAGGGATTTAGAAAAGTACAATGTGAAAATAAATTTAAATACAAAAATATCTTT
ACGAGATATTAAAGAAATGCAGTACGATAAAATTATTATAGCTACTGGTTCAAAACCAGCAAAAATGAATTTAGATACAG
ATATTGCACCTCTTACAGCCATTGAAGTACTAGAAGGCAACATTCCAGAGGGTAAAAATATAGCAATAATTGGAGGGGGA
CTTACAGGGCTTGAAACGGCTGAATATTTGGCAGAGAAAGGGAAAAAGGTGACAGTTTTTGAGATTAGAAGTGAAGTGGG
CGAAGGAATATATCCGATGGTGAAAAAGCTACTTCTTCAAAGACTGGAAAATTTGAAAGTCAATATAGTGACTGATGCCG
AAGTGAAGGATATATCAAAAGGAAAACTTATGTACAAGGTAAAGGATAGATTTGATGTAATTGAGGTAGAGGCGGTAGTA
TTGGCGGTTGGCAATGTTTCAGATGAAGAATTTTCAGAACTAAAAGGCGATGAGAGAATTTATTTCATTGGGGATTGTAA
AGAAGTAGCATCTGCGGTAGAGGCTATAAGGGAAGGGGCAGAAGTTTCACTTTTTATATGA

Upstream 100 bases:

>100_bases
ATCACCTCATAAGTATATGCTTATGTATTTAATAAAATTATACTATTTTACTTATAGTTGTTCAATCGCTACAATATAAA
ATGTGGTGAGGTGATTTAAA

Downstream 100 bases:

>100_bases
GAGGTGAGACTAGTGTTGATAAAAGGAGAATATGTTGATATAAAAGGCAGTAAGATTCATTATTTAGAATTAGGAAGAGA
TGACACACAAGACCTTGTAA

Product: NADH:flavin oxidoreductase

Products: trans-transtetradehydroacyl-CoA; NADPH [C]

Alternate protein names: NA

Number of amino acids: Translated: 606; Mature: 606

Protein sequence:

>606_residues
MLLKEGKIGGLHLKNRIVMLPTVTNLSKDGYVTEKEIEYYRRRSRSVALVIVGASFVNPLGKFFPNQIGIDADDKIEGLS
KLAEAIHQNGAKAAIQLAMHNPKYKPTDFPKEKIKNFVKDFVRGAIRAKKADFDAVELHFAHGWFVSQFLSPNTNKREDE
YGGSLEGRVRFAVEILKEIKDALPDFTVICRINGDDFTDGGFNIKESIEFAKILEKNGASAIDVSSGVSSTSEYHISPMG
IEDRPLLPFTKKIKESISIPVIAANKLGDVYDWERILEERIADFIGIARGLIGDPDLAEKLIKGEETDIRYCIHCNQACI
AYIQKGLSVSCMINPEVGREREFEVKTDKPLNIAVIGGGPAGMSAAKYLARKGHNVTLFEKENRLGGQLNVAQIPPHKQE
IGRVIEYLKRDLEKYNVKINLNTKISLRDIKEMQYDKIIIATGSKPAKMNLDTDIAPLTAIEVLEGNIPEGKNIAIIGGG
LTGLETAEYLAEKGKKVTVFEIRSEVGEGIYPMVKKLLLQRLENLKVNIVTDAEVKDISKGKLMYKVKDRFDVIEVEAVV
LAVGNVSDEEFSELKGDERIYFIGDCKEVASAVEAIREGAEVSLFI

Sequences:

>Translated_606_residues
MLLKEGKIGGLHLKNRIVMLPTVTNLSKDGYVTEKEIEYYRRRSRSVALVIVGASFVNPLGKFFPNQIGIDADDKIEGLS
KLAEAIHQNGAKAAIQLAMHNPKYKPTDFPKEKIKNFVKDFVRGAIRAKKADFDAVELHFAHGWFVSQFLSPNTNKREDE
YGGSLEGRVRFAVEILKEIKDALPDFTVICRINGDDFTDGGFNIKESIEFAKILEKNGASAIDVSSGVSSTSEYHISPMG
IEDRPLLPFTKKIKESISIPVIAANKLGDVYDWERILEERIADFIGIARGLIGDPDLAEKLIKGEETDIRYCIHCNQACI
AYIQKGLSVSCMINPEVGREREFEVKTDKPLNIAVIGGGPAGMSAAKYLARKGHNVTLFEKENRLGGQLNVAQIPPHKQE
IGRVIEYLKRDLEKYNVKINLNTKISLRDIKEMQYDKIIIATGSKPAKMNLDTDIAPLTAIEVLEGNIPEGKNIAIIGGG
LTGLETAEYLAEKGKKVTVFEIRSEVGEGIYPMVKKLLLQRLENLKVNIVTDAEVKDISKGKLMYKVKDRFDVIEVEAVV
LAVGNVSDEEFSELKGDERIYFIGDCKEVASAVEAIREGAEVSLFI
>Mature_606_residues
MLLKEGKIGGLHLKNRIVMLPTVTNLSKDGYVTEKEIEYYRRRSRSVALVIVGASFVNPLGKFFPNQIGIDADDKIEGLS
KLAEAIHQNGAKAAIQLAMHNPKYKPTDFPKEKIKNFVKDFVRGAIRAKKADFDAVELHFAHGWFVSQFLSPNTNKREDE
YGGSLEGRVRFAVEILKEIKDALPDFTVICRINGDDFTDGGFNIKESIEFAKILEKNGASAIDVSSGVSSTSEYHISPMG
IEDRPLLPFTKKIKESISIPVIAANKLGDVYDWERILEERIADFIGIARGLIGDPDLAEKLIKGEETDIRYCIHCNQACI
AYIQKGLSVSCMINPEVGREREFEVKTDKPLNIAVIGGGPAGMSAAKYLARKGHNVTLFEKENRLGGQLNVAQIPPHKQE
IGRVIEYLKRDLEKYNVKINLNTKISLRDIKEMQYDKIIIATGSKPAKMNLDTDIAPLTAIEVLEGNIPEGKNIAIIGGG
LTGLETAEYLAEKGKKVTVFEIRSEVGEGIYPMVKKLLLQRLENLKVNIVTDAEVKDISKGKLMYKVKDRFDVIEVEAVV
LAVGNVSDEEFSELKGDERIYFIGDCKEVASAVEAIREGAEVSLFI

Specific function: Reduces a range of alternative electron acceptors [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the N-terminal section; belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1789463, Length=519, Percent_Identity=30.2504816955684, Blast_Score=247, Evalue=2e-66,
Organism=Escherichia coli, GI1787939, Length=346, Percent_Identity=25.7225433526012, Blast_Score=98, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17559802, Length=267, Percent_Identity=28.0898876404494, Blast_Score=100, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI72001454, Length=217, Percent_Identity=29.9539170506912, Blast_Score=93, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17564188, Length=207, Percent_Identity=28.9855072463768, Blast_Score=92, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17540738, Length=263, Percent_Identity=27.3764258555133, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17566914, Length=212, Percent_Identity=30.6603773584906, Blast_Score=90, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17565138, Length=206, Percent_Identity=29.126213592233, Blast_Score=90, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17559804, Length=268, Percent_Identity=25.7462686567164, Blast_Score=87, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6321973, Length=362, Percent_Identity=27.6243093922652, Blast_Score=102, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6325086, Length=216, Percent_Identity=28.7037037037037, Blast_Score=91, Evalue=4e-19,
Organism=Drosophila melanogaster, GI28574881, Length=192, Percent_Identity=29.6875, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24665539, Length=192, Percent_Identity=29.6875, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24665547, Length=192, Percent_Identity=29.6875, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24665543, Length=192, Percent_Identity=29.6875, Blast_Score=74, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR013027
- InterPro:   IPR016040
- InterPro:   IPR001155
- InterPro:   IPR001327
- InterPro:   IPR000103 [H]

Pfam domain/function: PF00724 Oxidored_FMN; PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.3.1.34 [C]

Molecular weight: Translated: 67261; Mature: 67261

Theoretical pI: Translated: 6.46; Mature: 6.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLKEGKIGGLHLKNRIVMLPTVTNLSKDGYVTEKEIEYYRRRSRSVALVIVGASFVNPL
CCCCCCCCCCEEECCEEEEEEEECCCCCCCCCCHHHHHHHHHCCCCEEEEEECCHHHHHH
GKFFPNQIGIDADDKIEGLSKLAEAIHQNGAKAAIQLAMHNPKYKPTDFPKEKIKNFVKD
HHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHH
FVRGAIRAKKADFDAVELHFAHGWFVSQFLSPNTNKREDEYGGSLEGRVRFAVEILKEIK
HHHHHHHHHCCCCCEEEEEEEHHHHHHHHCCCCCCCCCHHCCCCCCCHHHHHHHHHHHHH
DALPDFTVICRINGDDFTDGGFNIKESIEFAKILEKNGASAIDVSSGVSSTSEYHISPMG
HHCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCEEEEECC
IEDRPLLPFTKKIKESISIPVIAANKLGDVYDWERILEERIADFIGIARGLIGDPDLAEK
CCCCCCCHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
LIKGEETDIRYCIHCNQACIAYIQKGLSVSCMINPEVGREREFEVKTDKPLNIAVIGGGP
HHCCCCCCEEEEEECCHHHHHHHHCCCEEEEEECCCCCCCCCEEEECCCCEEEEEECCCC
AGMSAAKYLARKGHNVTLFEKENRLGGQLNVAQIPPHKQEIGRVIEYLKRDLEKYNVKIN
CCHHHHHHHHHCCCCEEEEECCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHEEEEEE
LNTKISLRDIKEMQYDKIIIATGSKPAKMNLDTDIAPLTAIEVLEGNIPEGKNIAIIGGG
ECCEEEHHHHHHCCCCEEEEEECCCCCEECCCCCCCHHHHHHHHCCCCCCCCEEEEEECC
LTGLETAEYLAEKGKKVTVFEIRSEVGEGIYPMVKKLLLQRLENLKVNIVTDAEVKDISK
CCHHHHHHHHHHCCCEEEEEEEHHHHCCCHHHHHHHHHHHHHCCCEEEEEECCHHHCCCC
GKLMYKVKDRFDVIEVEAVVLAVGNVSDEEFSELKGDERIYFIGDCKEVASAVEAIREGA
CEEEEEECCCCCEEEEEEEEEEECCCCHHHHHHCCCCCEEEEEECHHHHHHHHHHHHCCC
EVSLFI
CEEEEC
>Mature Secondary Structure
MLLKEGKIGGLHLKNRIVMLPTVTNLSKDGYVTEKEIEYYRRRSRSVALVIVGASFVNPL
CCCCCCCCCCEEECCEEEEEEEECCCCCCCCCCHHHHHHHHHCCCCEEEEEECCHHHHHH
GKFFPNQIGIDADDKIEGLSKLAEAIHQNGAKAAIQLAMHNPKYKPTDFPKEKIKNFVKD
HHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHH
FVRGAIRAKKADFDAVELHFAHGWFVSQFLSPNTNKREDEYGGSLEGRVRFAVEILKEIK
HHHHHHHHHCCCCCEEEEEEEHHHHHHHHCCCCCCCCCHHCCCCCCCHHHHHHHHHHHHH
DALPDFTVICRINGDDFTDGGFNIKESIEFAKILEKNGASAIDVSSGVSSTSEYHISPMG
HHCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCEEEEECC
IEDRPLLPFTKKIKESISIPVIAANKLGDVYDWERILEERIADFIGIARGLIGDPDLAEK
CCCCCCCHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
LIKGEETDIRYCIHCNQACIAYIQKGLSVSCMINPEVGREREFEVKTDKPLNIAVIGGGP
HHCCCCCCEEEEEECCHHHHHHHHCCCEEEEEECCCCCCCCCEEEECCCCEEEEEECCCC
AGMSAAKYLARKGHNVTLFEKENRLGGQLNVAQIPPHKQEIGRVIEYLKRDLEKYNVKIN
CCHHHHHHHHHCCCCEEEEECCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHEEEEEE
LNTKISLRDIKEMQYDKIIIATGSKPAKMNLDTDIAPLTAIEVLEGNIPEGKNIAIIGGG
ECCEEEHHHHHHCCCCEEEEEECCCCCEECCCCCCCHHHHHHHHCCCCCCCCEEEEEECC
LTGLETAEYLAEKGKKVTVFEIRSEVGEGIYPMVKKLLLQRLENLKVNIVTDAEVKDISK
CCHHHHHHHHHHCCCEEEEEEEHHHHCCCHHHHHHHHHHHHHCCCEEEEEECCHHHCCCC
GKLMYKVKDRFDVIEVEAVVLAVGNVSDEEFSELKGDERIYFIGDCKEVASAVEAIREGA
CEEEEEECCCCCEEEEEEEEEEECCCCHHHHHHCCCCCEEEEEECHHHHHHHHHHHHCCC
EVSLFI
CEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: transDidehydroacyl-CoA; NADP+ [C]

Specific reaction: transDidehydroacyl-CoA + NADP+ =trans-transtetradehydroacyl-CoA + NADPH [C]

General reaction: Redox reaction [C]

Inhibitor: Iodoacetic acid; N-Ethyl maleimide [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8357835 [H]