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The map label for this gene is rsmE [H]

Identifier: 20807439

GI number: 20807439

Start: 962906

End: 963643

Strand: Direct

Name: rsmE [H]

Synonym: TTE0958

Alternate gene names: 20807439

Gene position: 962906-963643 (Clockwise)

Preceding gene: 20807438

Following gene: 20807440

Centisome position: 35.8

GC content: 36.18

Gene sequence:

>738_bases
ATGAGGAAGATCTTTGTTGAAAAGGGCAAAATAAAGGGAAAATTTGCCTATGTAGATGGGAAGGATTTTCACCATCTCAT
TCATGTATTAAGGTATAAGGTGGGAGATAAAATTGTGGTATCTGATATGGCAGAGGAGCATCTGGGAAGGATTGAAAAAA
TAGAAAAAGATAGGGCTATTTTATTTTTAGAAGAAAAAATGGAGGGCAATGCAGAAAGTCCTCTAGAAGTTTTTTTATTT
CAAGGGATACTCAAAGCTGACAAGATGGACCTTATAGTTCAAAAATGTACGGAAATAGGGGTAAAAAGGATTATACCTTT
TGTAAGTGAATTTTCTGTTGTGGATATAAAGAGAGCTAACATAGATAAAAAAGTTGAAAGGTGGAAAAAAATTAGCGAAG
AGGCTTGCAAGCAATCAGGAAGAAATATTATTCCTGAAATTTCTGCACCTGTATCTTTTAAAGAAGCGATAGAGAAAGCA
ATGGAGTGCGACCTTGCGATAATCCCTTATGAAAAAGAAGAAAAGAAAAGATTAAAAGAAGTGCTAAAAGATGTCGAAGA
TGCAAAGAAAATAGGGATATTCATAGGTCCTGAAGGAGGTTTTTCACAGAAGGAAATTGAGAAGGCTATAAATAATGGAA
TTGTACCCGTTACTCTAGGGCCTAGGATTTTGAGGGCAGAAACGGCAGCCATTGCAGTTTCTGCTATAGTTATGTACCAG
CTTGGAGACATGGGGTAA

Upstream 100 bases:

>100_bases
TTGAAGAAGTGAAAGAAAGAGCAGAGGAATTTTTTGAAATAATTGATATGAAAGAAGAAAAAGAGTGGTTGAGCATATTG
ATGAAGAAAAAGGGATAGAA

Downstream 100 bases:

>100_bases
ACCTGAAGGATAAGGGTAGTAAATTTTGATTTTTTGTGGTAAAATAAAAGGAGAAATATGAGATTTGAGGAAAGTAGTGA
CTAGAATAAAAGAAAGGAGT

Product: 16S ribosomal RNA methyltransferase RsmE

Products: NA

Alternate protein names: 16S rRNA m3U1498 methyltransferase [H]

Number of amino acids: Translated: 245; Mature: 245

Protein sequence:

>245_residues
MRKIFVEKGKIKGKFAYVDGKDFHHLIHVLRYKVGDKIVVSDMAEEHLGRIEKIEKDRAILFLEEKMEGNAESPLEVFLF
QGILKADKMDLIVQKCTEIGVKRIIPFVSEFSVVDIKRANIDKKVERWKKISEEACKQSGRNIIPEISAPVSFKEAIEKA
MECDLAIIPYEKEEKKRLKEVLKDVEDAKKIGIFIGPEGGFSQKEIEKAINNGIVPVTLGPRILRAETAAIAVSAIVMYQ
LGDMG

Sequences:

>Translated_245_residues
MRKIFVEKGKIKGKFAYVDGKDFHHLIHVLRYKVGDKIVVSDMAEEHLGRIEKIEKDRAILFLEEKMEGNAESPLEVFLF
QGILKADKMDLIVQKCTEIGVKRIIPFVSEFSVVDIKRANIDKKVERWKKISEEACKQSGRNIIPEISAPVSFKEAIEKA
MECDLAIIPYEKEEKKRLKEVLKDVEDAKKIGIFIGPEGGFSQKEIEKAINNGIVPVTLGPRILRAETAAIAVSAIVMYQ
LGDMG
>Mature_245_residues
MRKIFVEKGKIKGKFAYVDGKDFHHLIHVLRYKVGDKIVVSDMAEEHLGRIEKIEKDRAILFLEEKMEGNAESPLEVFLF
QGILKADKMDLIVQKCTEIGVKRIIPFVSEFSVVDIKRANIDKKVERWKKISEEACKQSGRNIIPEISAPVSFKEAIEKA
MECDLAIIPYEKEEKKRLKEVLKDVEDAKKIGIFIGPEGGFSQKEIEKAINNGIVPVTLGPRILRAETAAIAVSAIVMYQ
LGDMG

Specific function: Specifically methylates the uridine in position 1498 of 16S rRNA in the fully assembled 30S ribosomal subunit [H]

COG id: COG1385

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase RsmE family [H]

Homologues:

Organism=Escherichia coli, GI87082186, Length=175, Percent_Identity=32.5714285714286, Blast_Score=100, Evalue=6e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015947
- InterPro:   IPR006700 [H]

Pfam domain/function: PF04452 Methyltrans_RNA [H]

EC number: =2.1.1.193 [H]

Molecular weight: Translated: 27670; Mature: 27670

Theoretical pI: Translated: 8.44; Mature: 8.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKIFVEKGKIKGKFAYVDGKDFHHLIHVLRYKVGDKIVVSDMAEEHLGRIEKIEKDRAI
CCCEEEECCCCCEEEEEECCCHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHCCCCEE
LFLEEKMEGNAESPLEVFLFQGILKADKMDLIVQKCTEIGVKRIIPFVSEFSVVDIKRAN
EEEEHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHCC
IDKKVERWKKISEEACKQSGRNIIPEISAPVSFKEAIEKAMECDLAIIPYEKEEKKRLKE
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCEEEECCCHHHHHHHHH
VLKDVEDAKKIGIFIGPEGGFSQKEIEKAINNGIVPVTLGPRILRAETAAIAVSAIVMYQ
HHHHHHHHHHEEEEECCCCCCCHHHHHHHHHCCEEEEEECCHHHHHHHHHHHHHHHHHHH
LGDMG
HCCCC
>Mature Secondary Structure
MRKIFVEKGKIKGKFAYVDGKDFHHLIHVLRYKVGDKIVVSDMAEEHLGRIEKIEKDRAI
CCCEEEECCCCCEEEEEECCCHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHCCCCEE
LFLEEKMEGNAESPLEVFLFQGILKADKMDLIVQKCTEIGVKRIIPFVSEFSVVDIKRAN
EEEEHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHCC
IDKKVERWKKISEEACKQSGRNIIPEISAPVSFKEAIEKAMECDLAIIPYEKEEKKRLKE
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCEEEECCCHHHHHHHHH
VLKDVEDAKKIGIFIGPEGGFSQKEIEKAINNGIVPVTLGPRILRAETAAIAVSAIVMYQ
HHHHHHHHHHEEEEECCCCCCCHHHHHHHHHCCEEEEEECCHHHHHHHHHHHHHHHHHHH
LGDMG
HCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969508; 9023197; 9384377 [H]