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The map label for this gene is ComEC [H]

Identifier: 20807422

GI number: 20807422

Start: 942443

End: 944722

Strand: Direct

Name: ComEC [H]

Synonym: TTE0941

Alternate gene names: 20807422

Gene position: 942443-944722 (Clockwise)

Preceding gene: 20807421

Following gene: 20807423

Centisome position: 35.04

GC content: 36.45

Gene sequence:

>2280_bases
ATGGAAGCACCTTTTTTGTATATAACTACTTTTTTTTCTTTAGGTATTTTGCTTGGAAGGTTTTTATTTTTGCCTTATGT
TTTTCTTACTTTAATGGCGGCTTCAGTGGTTGTCTTGTTTTTTGTTTATAAAAAGGGTAAAAACGTGAGTTTGCTTTTGA
TTTTGCCTTTTGCACTTTTAGGTGCTATTTTGACTGGATTTGCGCTTAATGCTCCTAATCCATATGAAAGGCTAGATGGA
AAATTTATTGCCATAAAAGGGAAGGTTTGCGATGTTAAAGAGGAAGAAGGATACGCAAAGTATGTGGTAAAGCCTGAAAA
TTTGCCCAAGATATTAATAACCCAGTACGGGGGAGAATATCCTAAAAAAGGGGATATTGTGGAAGTGAGAGGAATTGTCT
CACTTCCGAAGCCCAGCTCAAATCCAGGAGGATTCGATTATCAGCTTTTTTTGAGGAAAAAAGGAATTTATGCAGTTATG
CGCATCCATCCCTATGGTACTTCTATTATAGGAAGAAGAGCAAATCTATTTGAGGAAATTCTCTGGAGGGCTCAAGAAAG
GATAAAAGAAAATTTTTTAGCTTCCATGCCTAAAAAGGACGCTCAGTTTCTCATTACCAGTTTTTTAGGTGAAAAAGTTC
TAGATGACCAAATTTTGACACAATTTCGCACAGTGGGGATATCCTACATCACCGCAGTTGCAGGGATGCAGGTGGCTATA
ATTTCCGGATTTGTCCTTTACATCTTGAGTTTGGTTCATTTGACCAGGTACAGAGCTTTTATTTTGCTGCCTGTTTTGAT
TTTTTATGCTTTTCTTACAGGACTAAATCCCCCTGTCATAAGAGCGGTTGTAATGGCATCTATTGCTGTTATTGGAGCCA
CTTACGGGAAAAATAAAAATTCAATTAATTCTCTTTCTTTTGCTGCTTTTTTAATACTCCTCTTTAATCCTTTAATGCTG
TGGGATGTGGGCTTTCAGCTTTCTTTTGTAGCGACTCTTGCTATCTTGTACTTTTACAGGCCCATTGATGAAAGGCTTTT
TAAATTGCCATCTTATTTGAAGGGCATAATAGCGTTAAATTTGTCTGCACAAATAGGCACGATTCCTTTTGTTATGTATT
ATTTTCACTACATTTCCTTTGTATCTTTTTTGTCAAACATTGCAATAGTTCCCCTTGTAAACATAGCTATAATCCTTAGC
TTTTTATCGGCTTTTCTAGGATTTGTATTTCCGACTGCATCAGTTTTTGTAAATTACATAAACATACCTTTTATAGAATT
GCTTTTCAAGGTAACATCCTTTTTTGAAAAATTTCCTTACGCCAGTGTAAATGTTTTTATCCCTCCAGTAATCGTTATAG
TGCTTTATTACATTGTTTTAGGAATTTTGATAAGTAGAATTGAGTTGAGGAAAAAGAAATTAGCTGTGGCGGCGTCTTTT
ATGGTAATTGCTTTACTATTTTTCTGGAATTACTTTGTTCCGAAAGATTTGGAAGTGACTTTTTTGGACGTTGGGCAGGG
GGATGCTACCTTTGTGAGGACGCCTCATGGGAGAGCTTTTTTAATAGACGGAGGTGGGAATCCTGTTTTTTCTAACTCTT
CTTTTGATATAGGAGAAGACATACTCCTTCCATTTTTGTACTATAAAGGTGTCATTAAACTGGATGCTGTTTTTATTTCT
CACACAGATATTGACCACATAGGTGGAATTATAACGGTTTTGGAGAATATGAAGGTGGATAGAGTATTCATAGGGGTTCA
GAAAGTAGAGGACCAAAATTTTAAAAAGATGATGGAGGTTGCAGGTAAAAAGAGAGTTTCTGTAATTTTTTTGAAAAAGG
GAGATAAGGTAGAGGTAGACGGAGTAAAGGTGTATGTTCTTCATCCAGATCCGGGCAATTTAATAGAGGAGAATCCCATA
AATAACAATGCACTTGTGTTTAAGATGAGGTACGGAAATATAAGTTTTCTATTTACAGGAGATATAGAAAAGCCGGCAGA
AGAGGTTTTAAAGGAAAGCGATTTAAAAACGCACGTATTAAAAGTTGCCCATCACGGTTCCAGTACTTCTTCAACTCCTG
AGTTTATAGAAAAGGCAGATCCTCAGGTTTTTGTGATTCAGGTGGGGAAAAACAGTTATGGACTGCCGAGTGAGAGGGTG
GTAAAATATTTAAAAGAGAGAGGAAAGTTGTACAGGACAGATGAAAATGGTGCTGTAATTGTAAGGACAGATGGAGTAAA
GGCTCGCATATACCCCTTTTTAAAAGGAGTGAAAGAATGA

Upstream 100 bases:

>100_bases
CTGTCACCTCTTATATCGATAAAAGACTATTATGACGAAGCAAAAAAAGTTTTTGAAAATACAGAGATTGCGAGAGATTT
TGAAGTTTACGTGATCTAGT

Downstream 100 bases:

>100_bases
ACTATAGGGAATTTGTAGAAAGCATAAAAAAGGGACAGATAGCTCCTTTATACCTTTTTTACGGAGAAGAGAGATTTTTG
CTTTTAGATGCTGTTAAGAG

Product: hydrolase of metallo-beta-lactamase fold

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 759; Mature: 759

Protein sequence:

>759_residues
MEAPFLYITTFFSLGILLGRFLFLPYVFLTLMAASVVVLFFVYKKGKNVSLLLILPFALLGAILTGFALNAPNPYERLDG
KFIAIKGKVCDVKEEEGYAKYVVKPENLPKILITQYGGEYPKKGDIVEVRGIVSLPKPSSNPGGFDYQLFLRKKGIYAVM
RIHPYGTSIIGRRANLFEEILWRAQERIKENFLASMPKKDAQFLITSFLGEKVLDDQILTQFRTVGISYITAVAGMQVAI
ISGFVLYILSLVHLTRYRAFILLPVLIFYAFLTGLNPPVIRAVVMASIAVIGATYGKNKNSINSLSFAAFLILLFNPLML
WDVGFQLSFVATLAILYFYRPIDERLFKLPSYLKGIIALNLSAQIGTIPFVMYYFHYISFVSFLSNIAIVPLVNIAIILS
FLSAFLGFVFPTASVFVNYINIPFIELLFKVTSFFEKFPYASVNVFIPPVIVIVLYYIVLGILISRIELRKKKLAVAASF
MVIALLFFWNYFVPKDLEVTFLDVGQGDATFVRTPHGRAFLIDGGGNPVFSNSSFDIGEDILLPFLYYKGVIKLDAVFIS
HTDIDHIGGIITVLENMKVDRVFIGVQKVEDQNFKKMMEVAGKKRVSVIFLKKGDKVEVDGVKVYVLHPDPGNLIEENPI
NNNALVFKMRYGNISFLFTGDIEKPAEEVLKESDLKTHVLKVAHHGSSTSSTPEFIEKADPQVFVIQVGKNSYGLPSERV
VKYLKERGKLYRTDENGAVIVRTDGVKARIYPFLKGVKE

Sequences:

>Translated_759_residues
MEAPFLYITTFFSLGILLGRFLFLPYVFLTLMAASVVVLFFVYKKGKNVSLLLILPFALLGAILTGFALNAPNPYERLDG
KFIAIKGKVCDVKEEEGYAKYVVKPENLPKILITQYGGEYPKKGDIVEVRGIVSLPKPSSNPGGFDYQLFLRKKGIYAVM
RIHPYGTSIIGRRANLFEEILWRAQERIKENFLASMPKKDAQFLITSFLGEKVLDDQILTQFRTVGISYITAVAGMQVAI
ISGFVLYILSLVHLTRYRAFILLPVLIFYAFLTGLNPPVIRAVVMASIAVIGATYGKNKNSINSLSFAAFLILLFNPLML
WDVGFQLSFVATLAILYFYRPIDERLFKLPSYLKGIIALNLSAQIGTIPFVMYYFHYISFVSFLSNIAIVPLVNIAIILS
FLSAFLGFVFPTASVFVNYINIPFIELLFKVTSFFEKFPYASVNVFIPPVIVIVLYYIVLGILISRIELRKKKLAVAASF
MVIALLFFWNYFVPKDLEVTFLDVGQGDATFVRTPHGRAFLIDGGGNPVFSNSSFDIGEDILLPFLYYKGVIKLDAVFIS
HTDIDHIGGIITVLENMKVDRVFIGVQKVEDQNFKKMMEVAGKKRVSVIFLKKGDKVEVDGVKVYVLHPDPGNLIEENPI
NNNALVFKMRYGNISFLFTGDIEKPAEEVLKESDLKTHVLKVAHHGSSTSSTPEFIEKADPQVFVIQVGKNSYGLPSERV
VKYLKERGKLYRTDENGAVIVRTDGVKARIYPFLKGVKE
>Mature_759_residues
MEAPFLYITTFFSLGILLGRFLFLPYVFLTLMAASVVVLFFVYKKGKNVSLLLILPFALLGAILTGFALNAPNPYERLDG
KFIAIKGKVCDVKEEEGYAKYVVKPENLPKILITQYGGEYPKKGDIVEVRGIVSLPKPSSNPGGFDYQLFLRKKGIYAVM
RIHPYGTSIIGRRANLFEEILWRAQERIKENFLASMPKKDAQFLITSFLGEKVLDDQILTQFRTVGISYITAVAGMQVAI
ISGFVLYILSLVHLTRYRAFILLPVLIFYAFLTGLNPPVIRAVVMASIAVIGATYGKNKNSINSLSFAAFLILLFNPLML
WDVGFQLSFVATLAILYFYRPIDERLFKLPSYLKGIIALNLSAQIGTIPFVMYYFHYISFVSFLSNIAIVPLVNIAIILS
FLSAFLGFVFPTASVFVNYINIPFIELLFKVTSFFEKFPYASVNVFIPPVIVIVLYYIVLGILISRIELRKKKLAVAASF
MVIALLFFWNYFVPKDLEVTFLDVGQGDATFVRTPHGRAFLIDGGGNPVFSNSSFDIGEDILLPFLYYKGVIKLDAVFIS
HTDIDHIGGIITVLENMKVDRVFIGVQKVEDQNFKKMMEVAGKKRVSVIFLKKGDKVEVDGVKVYVLHPDPGNLIEENPI
NNNALVFKMRYGNISFLFTGDIEKPAEEVLKESDLKTHVLKVAHHGSSTSSTPEFIEKADPQVFVIQVGKNSYGLPSERV
VKYLKERGKLYRTDENGAVIVRTDGVKARIYPFLKGVKE

Specific function: The comE operon is required for the binding and uptake of transforming DNA. ComEC is required for internalization but is dispensable for DNA binding [H]

COG id: COG2333

COG function: function code R; Predicted hydrolase (metallo-beta-lactamase superfamily)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: To H.influenzae REC2, N.gonorrhoeae ComA and E.coli YcaI [H]

Homologues:

Organism=Escherichia coli, GI87081801, Length=692, Percent_Identity=22.2543352601156, Blast_Score=139, Evalue=6e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279
- InterPro:   IPR004477
- InterPro:   IPR004797 [H]

Pfam domain/function: PF03772 Competence; PF00753 Lactamase_B [H]

EC number: NA

Molecular weight: Translated: 85240; Mature: 85240

Theoretical pI: Translated: 9.78; Mature: 9.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEAPFLYITTFFSLGILLGRFLFLPYVFLTLMAASVVVLFFVYKKGKNVSLLLILPFALL
CCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHH
GAILTGFALNAPNPYERLDGKFIAIKGKVCDVKEEEGYAKYVVKPENLPKILITQYGGEY
HHHHHCCCCCCCCHHHHCCCEEEEEECCEECCCCCCCCEEEEECCCCCCEEEEECCCCCC
PKKGDIVEVRGIVSLPKPSSNPGGFDYQLFLRKKGIYAVMRIHPYGTSIIGRRANLFEEI
CCCCCEEEEEEEEECCCCCCCCCCEEEEEEEECCCEEEEEEEECCCCHHHHHHHHHHHHH
LWRAQERIKENFLASMPKKDAQFLITSFLGEKVLDDQILTQFRTVGISYITAVAGMQVAI
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHH
ISGFVLYILSLVHLTRYRAFILLPVLIFYAFLTGLNPPVIRAVVMASIAVIGATYGKNKN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCC
SINSLSFAAFLILLFNPLMLWDVGFQLSFVATLAILYFYRPIDERLFKLPSYLKGIIALN
CCHHHHHHHHHHHHHCHHHHHHCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHEEE
LSAQIGTIPFVMYYFHYISFVSFLSNIAIVPLVNIAIILSFLSAFLGFVFPTASVFVNYI
CCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NIPFIELLFKVTSFFEKFPYASVNVFIPPVIVIVLYYIVLGILISRIELRKKKLAVAASF
CCHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MVIALLFFWNYFVPKDLEVTFLDVGQGDATFVRTPHGRAFLIDGGGNPVFSNSSFDIGED
HHHHHHHHHHHCCCCCCEEEEEECCCCCEEEEECCCCCEEEEECCCCCCCCCCCCCCCHH
ILLPFLYYKGVIKLDAVFISHTDIDHIGGIITVLENMKVDRVFIGVQKVEDQNFKKMMEV
HHHHHHHHCCHHEEEEEEEECCCHHHHHHHHHHHHCCCCCEEEEEHHHHCCHHHHHHHHH
AGKKRVSVIFLKKGDKVEVDGVKVYVLHPDPGNLIEENPINNNALVFKMRYGNISFLFTG
CCCCEEEEEEEECCCEEEECCEEEEEEECCCCCCCCCCCCCCCEEEEEEEECCEEEEEEC
DIEKPAEEVLKESDLKTHVLKVAHHGSSTSSTPEFIEKADPQVFVIQVGKNSYGLPSERV
CCCHHHHHHHHHCCHHHHHHHHHHCCCCCCCCHHHHHCCCCCEEEEEECCCCCCCCHHHH
VKYLKERGKLYRTDENGAVIVRTDGVKARIYPFLKGVKE
HHHHHHCCCEEEECCCCEEEEEECCCCCHHHHHHHCCCC
>Mature Secondary Structure
MEAPFLYITTFFSLGILLGRFLFLPYVFLTLMAASVVVLFFVYKKGKNVSLLLILPFALL
CCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHH
GAILTGFALNAPNPYERLDGKFIAIKGKVCDVKEEEGYAKYVVKPENLPKILITQYGGEY
HHHHHCCCCCCCCHHHHCCCEEEEEECCEECCCCCCCCEEEEECCCCCCEEEEECCCCCC
PKKGDIVEVRGIVSLPKPSSNPGGFDYQLFLRKKGIYAVMRIHPYGTSIIGRRANLFEEI
CCCCCEEEEEEEEECCCCCCCCCCEEEEEEEECCCEEEEEEEECCCCHHHHHHHHHHHHH
LWRAQERIKENFLASMPKKDAQFLITSFLGEKVLDDQILTQFRTVGISYITAVAGMQVAI
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHH
ISGFVLYILSLVHLTRYRAFILLPVLIFYAFLTGLNPPVIRAVVMASIAVIGATYGKNKN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCC
SINSLSFAAFLILLFNPLMLWDVGFQLSFVATLAILYFYRPIDERLFKLPSYLKGIIALN
CCHHHHHHHHHHHHHCHHHHHHCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHEEE
LSAQIGTIPFVMYYFHYISFVSFLSNIAIVPLVNIAIILSFLSAFLGFVFPTASVFVNYI
CCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NIPFIELLFKVTSFFEKFPYASVNVFIPPVIVIVLYYIVLGILISRIELRKKKLAVAASF
CCHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MVIALLFFWNYFVPKDLEVTFLDVGQGDATFVRTPHGRAFLIDGGGNPVFSNSSFDIGED
HHHHHHHHHHHCCCCCCEEEEEECCCCCEEEEECCCCCEEEEECCCCCCCCCCCCCCCHH
ILLPFLYYKGVIKLDAVFISHTDIDHIGGIITVLENMKVDRVFIGVQKVEDQNFKKMMEV
HHHHHHHHCCHHEEEEEEEECCCHHHHHHHHHHHHCCCCCEEEEEHHHHCCHHHHHHHHH
AGKKRVSVIFLKKGDKVEVDGVKVYVLHPDPGNLIEENPINNNALVFKMRYGNISFLFTG
CCCCEEEEEEEECCCEEEECCEEEEEEECCCCCCCCCCCCCCCEEEEEEEECCEEEEEEC
DIEKPAEEVLKESDLKTHVLKVAHHGSSTSSTPEFIEKADPQVFVIQVGKNSYGLPSERV
CCCHHHHHHHHHCCHHHHHHHHHHCCCCCCCCHHHHHCCCCCEEEEEECCCCCCCCHHHH
VKYLKERGKLYRTDENGAVIVRTDGVKARIYPFLKGVKE
HHHHHHCCCEEEECCCCEEEEEECCCCCHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7968523; 8969508; 9384377 [H]