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The map label for this gene is NuoF [H]

Identifier: 20807374

GI number: 20807374

Start: 901629

End: 903419

Strand: Direct

Name: NuoF [H]

Synonym: TTE0893

Alternate gene names: 20807374

Gene position: 901629-903419 (Clockwise)

Preceding gene: 20807373

Following gene: 20807375

Centisome position: 33.52

GC content: 44.22

Gene sequence:

>1791_bases
ATGCTTTACAGGTCTCACGTAATGGTATGTGGTGGAACTGGCTGTACTTCATCAGGTTCTGACGAGGTAGCTGAACGTTT
CATAGAAGAGATTAAAAAAGCGGGTTTGGACAAAGAAATACTGGTTGTGAGGACAGGATGCTTTGGACTCTGCGAGCTGG
GTCCTGTTGTGGTAGTTTACCCGGAAGGGGTTTTTTACAGCAGAGTAAAGCCAGAGTATGTGCCTGAGATCGTAGAGGAG
CATCTTTTAAAGGGAAGACCTGTCAGAAAGTACATCTACGGTGAGAGCCTAGAAGAAAAGGCTATAAAGCCGTTGGAGGA
GACACCTTTCTTCAGAAAACAGAGAAGAATTGCTCTTAGAAACTGCGGTGTGATAAACCCTGAAGACATAAGAGAAGCTA
TTGCTTTTGACGGGTATAAAGCGCTTGCGAAGGTTCTTACAGAGATGACTCCTGAGCAAGTAATTGAAGAGGTCAAAAAA
TCAGGATTAAGGGGAAGAGGCGGTGGCGGATTCCCAACAGGTGTGAAATGGGAATTTGCTTACAAGCAGAAAGAAACCCC
TAAATATGTTGTGTGCAATGCTGATGAAGGAGACCCTGGCGCTTTCATGGATAGAAGCATACTGGAAGGAGACCCCCACA
GTGTTTTAGAAGCAATGGCAATAGCTGGATATGCAATAGGTGCAAATCACGGTTATATATACGTGAGAGCAGAGTATCCT
CTGGCTGTAAAAAGGCTTAAGATTGCGATTCAGCAGGCGAGAGAGTACGGACTTTTAGGAAAGGATATATTTGGCACAGG
GTTTGACTTTGACATTGAAATAAGGCTTGGAGCAGGTGCTTTTGTCTGCGGTGAAGAGACAGCTCTTTTAAACTCTATTA
TGGGTAAAAGAGGAGAGCCGAGGCCAAGACCCCCCTTCCCGGCGGTGAAGGGTGTATGGGGCAAACCCACAATTATAAAT
AACGTGGAGACTTTTGCAAATATTCCTCCCATCATATTAAACGGCGGAGAATGGTTCGCAAGCATTGGAACAGAAAAATC
TAAAGGAACGAAAGTTTTTGCTCTCACCGGTAAGGTGAATAACACCGGACTCATTGAAGTGCCTATGGGTACAACTTTAA
GAGAAATCATATACGAAATAGGCGGAGGAATACCCGGAGGAAAGAAATTCAAAGCTGTTCAGATAGGCGGTCCTTCTGGC
GGATGCATACCGGCAGAGCTTTTAGATACTCCGATTGATTATGATTCTTTAACCAGTGCAGGTGCGATGATGGGTTCTGG
CGGACTTGTTGTGATGGATGAAGACACATGTATGGTCAATGTGGCGAAGTTCTTCCTCGAGTTTACTGTCGATGAATCCT
GCGGTAAGTGTGCTCCTTGTAGGATTGGTACGAAGAGGATGCTGGAGCTTTTGGATAAGATAACTTCCGGAAAAGGAGAA
GAAGGAGATATTGAAAAGTTAGAAGAACTGGCAAAGACCATAAAGGCAACGGCTCTATGTGGTCTTGGACAGACAGCACC
CAATCCTGTACTTTCTACTTTGAGATATTTCAGGCACGAATATGAGGCCCACATAAAAGAAAAAAGGTGCCCTGCCGGTG
TATGTACAGCGCTTTTAAGCTTTGTTATAGATCCTGAAAAGTGCAAAGCCTGTGGAATTTGTGCAAAGAACTGTCCTGTT
GGAGCTATTTCTGGTAAGCCAAAGACGCCTTATGTGATTGACCAAGAGAAGTGTATTAAGTGCGGAACATGTATTGATAA
GTGTCCGTTTGGCGCAATATACAAAAAATAA

Upstream 100 bases:

>100_bases
TGAAAAGAAGGCAAGACAAATAGTAGTGGAACATGTGATAAATGGCCACCCAATCAGGGAATGGACTATAGAAAATTACG
AGTAAGAGGAGGGATATTAG

Downstream 100 bases:

>100_bases
AAGAGGAAGGAGTGTAAAAGGATGGACAAAGTTCGCGTTACAATAGATGGCATAACTGTAGAAGTGCCTTCCTACTATAC
GGTACTGGAAGCGGCAAAAG

Product: NADH:ubiquinone oxidoreductase, NADH-binding (51 kD) subunit

Products: NA

Alternate protein names: NADH dehydrogenase I subunit F 2; NDH-1 subunit F 2 [H]

Number of amino acids: Translated: 596; Mature: 596

Protein sequence:

>596_residues
MLYRSHVMVCGGTGCTSSGSDEVAERFIEEIKKAGLDKEILVVRTGCFGLCELGPVVVVYPEGVFYSRVKPEYVPEIVEE
HLLKGRPVRKYIYGESLEEKAIKPLEETPFFRKQRRIALRNCGVINPEDIREAIAFDGYKALAKVLTEMTPEQVIEEVKK
SGLRGRGGGGFPTGVKWEFAYKQKETPKYVVCNADEGDPGAFMDRSILEGDPHSVLEAMAIAGYAIGANHGYIYVRAEYP
LAVKRLKIAIQQAREYGLLGKDIFGTGFDFDIEIRLGAGAFVCGEETALLNSIMGKRGEPRPRPPFPAVKGVWGKPTIIN
NVETFANIPPIILNGGEWFASIGTEKSKGTKVFALTGKVNNTGLIEVPMGTTLREIIYEIGGGIPGGKKFKAVQIGGPSG
GCIPAELLDTPIDYDSLTSAGAMMGSGGLVVMDEDTCMVNVAKFFLEFTVDESCGKCAPCRIGTKRMLELLDKITSGKGE
EGDIEKLEELAKTIKATALCGLGQTAPNPVLSTLRYFRHEYEAHIKEKRCPAGVCTALLSFVIDPEKCKACGICAKNCPV
GAISGKPKTPYVIDQEKCIKCGTCIDKCPFGAIYKK

Sequences:

>Translated_596_residues
MLYRSHVMVCGGTGCTSSGSDEVAERFIEEIKKAGLDKEILVVRTGCFGLCELGPVVVVYPEGVFYSRVKPEYVPEIVEE
HLLKGRPVRKYIYGESLEEKAIKPLEETPFFRKQRRIALRNCGVINPEDIREAIAFDGYKALAKVLTEMTPEQVIEEVKK
SGLRGRGGGGFPTGVKWEFAYKQKETPKYVVCNADEGDPGAFMDRSILEGDPHSVLEAMAIAGYAIGANHGYIYVRAEYP
LAVKRLKIAIQQAREYGLLGKDIFGTGFDFDIEIRLGAGAFVCGEETALLNSIMGKRGEPRPRPPFPAVKGVWGKPTIIN
NVETFANIPPIILNGGEWFASIGTEKSKGTKVFALTGKVNNTGLIEVPMGTTLREIIYEIGGGIPGGKKFKAVQIGGPSG
GCIPAELLDTPIDYDSLTSAGAMMGSGGLVVMDEDTCMVNVAKFFLEFTVDESCGKCAPCRIGTKRMLELLDKITSGKGE
EGDIEKLEELAKTIKATALCGLGQTAPNPVLSTLRYFRHEYEAHIKEKRCPAGVCTALLSFVIDPEKCKACGICAKNCPV
GAISGKPKTPYVIDQEKCIKCGTCIDKCPFGAIYKK
>Mature_596_residues
MLYRSHVMVCGGTGCTSSGSDEVAERFIEEIKKAGLDKEILVVRTGCFGLCELGPVVVVYPEGVFYSRVKPEYVPEIVEE
HLLKGRPVRKYIYGESLEEKAIKPLEETPFFRKQRRIALRNCGVINPEDIREAIAFDGYKALAKVLTEMTPEQVIEEVKK
SGLRGRGGGGFPTGVKWEFAYKQKETPKYVVCNADEGDPGAFMDRSILEGDPHSVLEAMAIAGYAIGANHGYIYVRAEYP
LAVKRLKIAIQQAREYGLLGKDIFGTGFDFDIEIRLGAGAFVCGEETALLNSIMGKRGEPRPRPPFPAVKGVWGKPTIIN
NVETFANIPPIILNGGEWFASIGTEKSKGTKVFALTGKVNNTGLIEVPMGTTLREIIYEIGGGIPGGKKFKAVQIGGPSG
GCIPAELLDTPIDYDSLTSAGAMMGSGGLVVMDEDTCMVNVAKFFLEFTVDESCGKCAPCRIGTKRMLELLDKITSGKGE
EGDIEKLEELAKTIKATALCGLGQTAPNPVLSTLRYFRHEYEAHIKEKRCPAGVCTALLSFVIDPEKCKACGICAKNCPV
GAISGKPKTPYVIDQEKCIKCGTCIDKCPFGAIYKK

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1894

COG function: function code C; NADH:ubiquinone oxidoreductase, NADH-binding (51 kD) subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 51 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI20149568, Length=380, Percent_Identity=44.2105263157895, Blast_Score=325, Evalue=7e-89,
Organism=Homo sapiens, GI260656005, Length=394, Percent_Identity=43.6548223350254, Blast_Score=325, Evalue=8e-89,
Organism=Escherichia coli, GI1788620, Length=404, Percent_Identity=43.3168316831683, Blast_Score=360, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI17531727, Length=379, Percent_Identity=42.7440633245383, Blast_Score=313, Evalue=1e-85,
Organism=Drosophila melanogaster, GI19920794, Length=393, Percent_Identity=42.4936386768448, Blast_Score=317, Evalue=2e-86,
Organism=Drosophila melanogaster, GI45550452, Length=378, Percent_Identity=43.1216931216931, Blast_Score=311, Evalue=8e-85,
Organism=Drosophila melanogaster, GI24653819, Length=385, Percent_Identity=34.8051948051948, Blast_Score=247, Evalue=2e-65,
Organism=Drosophila melanogaster, GI24653817, Length=355, Percent_Identity=32.6760563380282, Blast_Score=212, Evalue=7e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001949
- InterPro:   IPR019575
- InterPro:   IPR011537
- InterPro:   IPR011538
- InterPro:   IPR019554 [H]

Pfam domain/function: PF01512 Complex1_51K; PF10589 NADH_4Fe-4S; PF10531 SLBB [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 64710; Mature: 64710

Theoretical pI: Translated: 7.38; Mature: 7.38

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00645 COMPLEX1_51K_2 ; PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.9 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
3.9 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLYRSHVMVCGGTGCTSSGSDEVAERFIEEIKKAGLDKEILVVRTGCFGLCELGPVVVVY
CCEECEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHCCCCEEEEC
PEGVFYSRVKPEYVPEIVEEHLLKGRPVRKYIYGESLEEKAIKPLEETPFFRKQRRIALR
CCCCEECCCCHHHHHHHHHHHHHCCCCHHHHHCCCCHHHHHCCCCHHCCHHHHHHHHHHH
NCGVINPEDIREAIAFDGYKALAKVLTEMTPEQVIEEVKKSGLRGRGGGGFPTGVKWEFA
HCCCCCHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCEEEEE
YKQKETPKYVVCNADEGDPGAFMDRSILEGDPHSVLEAMAIAGYAIGANHGYIYVRAEYP
ECCCCCCCEEEEECCCCCCCCHHCCHHHCCCHHHHHHHHHHHHEEEECCCCEEEEEECCC
LAVKRLKIAIQQAREYGLLGKDIFGTGFDFDIEIRLGAGAFVCGEETALLNSIMGKRGEP
HHHHHHHHHHHHHHHHCCCCCHHCCCCCCEEEEEEECCCEEEECCHHHHHHHHHCCCCCC
RPRPPFPAVKGVWGKPTIINNVETFANIPPIILNGGEWFASIGTEKSKGTKVFALTGKVN
CCCCCCCHHCCCCCCCCEECCHHHHHCCCCEEEECCCEEEECCCCCCCCCEEEEEECCCC
NTGLIEVPMGTTLREIIYEIGGGIPGGKKFKAVQIGGPSGGCIPAELLDTPIDYDSLTSA
CCCEEEECCCHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCHHHHCCCCCHHHHHHH
GAMMGSGGLVVMDEDTCMVNVAKFFLEFTVDESCGKCAPCRIGTKRMLELLDKITSGKGE
HHHCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCC
EGDIEKLEELAKTIKATALCGLGQTAPNPVLSTLRYFRHEYEAHIKEKRCPAGVCTALLS
CCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
FVIDPEKCKACGICAKNCPVGAISGKPKTPYVIDQEKCIKCGTCIDKCPFGAIYKK
HHCCHHHHHECCCCCCCCCCCCCCCCCCCCEEECHHHHHHHCCHHHHCCCCCCCCC
>Mature Secondary Structure
MLYRSHVMVCGGTGCTSSGSDEVAERFIEEIKKAGLDKEILVVRTGCFGLCELGPVVVVY
CCEECEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHCCCCEEEEC
PEGVFYSRVKPEYVPEIVEEHLLKGRPVRKYIYGESLEEKAIKPLEETPFFRKQRRIALR
CCCCEECCCCHHHHHHHHHHHHHCCCCHHHHHCCCCHHHHHCCCCHHCCHHHHHHHHHHH
NCGVINPEDIREAIAFDGYKALAKVLTEMTPEQVIEEVKKSGLRGRGGGGFPTGVKWEFA
HCCCCCHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCEEEEE
YKQKETPKYVVCNADEGDPGAFMDRSILEGDPHSVLEAMAIAGYAIGANHGYIYVRAEYP
ECCCCCCCEEEEECCCCCCCCHHCCHHHCCCHHHHHHHHHHHHEEEECCCCEEEEEECCC
LAVKRLKIAIQQAREYGLLGKDIFGTGFDFDIEIRLGAGAFVCGEETALLNSIMGKRGEP
HHHHHHHHHHHHHHHHCCCCCHHCCCCCCEEEEEEECCCEEEECCHHHHHHHHHCCCCCC
RPRPPFPAVKGVWGKPTIINNVETFANIPPIILNGGEWFASIGTEKSKGTKVFALTGKVN
CCCCCCCHHCCCCCCCCEECCHHHHHCCCCEEEECCCEEEECCCCCCCCCEEEEEECCCC
NTGLIEVPMGTTLREIIYEIGGGIPGGKKFKAVQIGGPSGGCIPAELLDTPIDYDSLTSA
CCCEEEECCCHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCHHHHCCCCCHHHHHHH
GAMMGSGGLVVMDEDTCMVNVAKFFLEFTVDESCGKCAPCRIGTKRMLELLDKITSGKGE
HHHCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCC
EGDIEKLEELAKTIKATALCGLGQTAPNPVLSTLRYFRHEYEAHIKEKRCPAGVCTALLS
CCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
FVIDPEKCKACGICAKNCPVGAISGKPKTPYVIDQEKCIKCGTCIDKCPFGAIYKK
HHCCHHHHHECCCCCCCCCCCCCCCCCCCCEEECHHHHHHHCCHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481432 [H]