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The map label for this gene is OppA7 [H]

Identifier: 20807359

GI number: 20807359

Start: 887587

End: 889173

Strand: Direct

Name: OppA7 [H]

Synonym: TTE0878

Alternate gene names: 20807359

Gene position: 887587-889173 (Clockwise)

Preceding gene: 20807358

Following gene: 20807360

Centisome position: 33.0

GC content: 35.48

Gene sequence:

>1587_bases
ATGAAAAGATTTCTGGCTTTTGTTTTTATTTTTGCATTTTTACTGACCGGCTGTAAAATTAATTCTCCAGATATTACTGC
AATTCCTGAAAAGAAGGAAAGTAAGAAAGAAGCCTCTCAGGAAGAAATTCAGGAAGGCGGAACTTTGAGGGTTAATATAA
CTTCTTTTGACACTTTAAATCCTTTTTTGAATGACAATGAAATGGTAAGACAGCTTCTCAATTTAACCCTCGAACCGCTT
GTTACACTTGATAAAAGTTTAAAGCCTATTCCTCTTCTTGCCTATAAATGGGAAGTAAATGGATTAAATATAAAATTTTA
TCTGAGGAAAAATATAAAGTGGCAGGACGGGGAGAGTTTTACAGCAAGAGATGTGAAATTTACAATGGATTCTTTCCTTG
ACAAAGGGTCCAAAAGCCCTTATAAAGATTTAATAAAGGCGCACATCTCTTCCTACAGAGAAATAGGCGATTATGAGTTT
GAAGTAAGTTTAAACGGACCTGTGGCGAATCCTTTGGCTTTATTTATTTTTCCAATTGTGGCTGAGCACCAGTACAAAGA
CAAAGGAGATATTGTAAACCCTCTACTAGTTCCTATTGGGACGGGTCCATATAAGATTGTCTCTTACGGTCAAGGCAATT
CTGTTGTTTTAAAGAGAAATCCGCATTACTGGGGGGAGAAACCTCATTTAGAAGAGATAAATTTTAAAATAGTGTCTGAT
GAAAATGCCATGATAACTTCTTTTCTGAGCAAAGAAGCAGATTTTACTTTTATTAAGGATGTGGACTGGGATAAGTACAG
GGAGAATTCTAGTATAAACATATACAAGTACACGCTGCAAAGGTACGTATTTTTGGCACCAAATCTAAAAAAACAGGTAT
TTTCAGATGTGGCTGTGAGAAAGGCTATAGCCTATGGAATAGATGTGGACAGGATTTTAAGAGAGGTTTATTTTAAGCAC
GGATTAAAATCTAATGTTCCGATAAGACCTGATTCCTGGCTTTATAGCCCTAAGATCGCTTCTGTCTACAGCATAAAAGA
AGCTGATAAGGTGCTACAGGATGCTGGGTGGTTAAAGGCAAACGGGATAGGCACAAAAGGCGGATATCAGCTTAAGTTTA
ATTTGCTAGTAAATTCTGACAATCCATACCTTTTAAAAGTGGCGCAGATTATAAAGACCAATCTAAAAGAAATTGGAATT
GATATAAATGTAGTCCCTGCTTCATGGGATGACTTGCTTAAATCTGTTTTTTCAGGGAATTTTGATTTGGCTTTAATGGA
GTGGAACTTAAACTATAATCAGGATATTTCTTCCATGTTTATGACGAGAGGGAAAGACAATTTTATGGGATATAGCAATC
CATCACTTGATGACCTCTTTAACAAAGTATTTTCAGAAACAGATGAAAAAAGTTTAAGAAATAACTATTATAAGGTAGGG
GAATTTTTGGCTGAAGAACAGCCAGTGATAGGGCTTTTTTACTTAGAAGGCGCCGTTATGGCCTATGGTAATGTAAAAGG
GATTGACCCCACTTCTTTTAATGTTTTTAACAATATTTCTAGTTGGTATTTAAAAAAACAGTATTGA

Upstream 100 bases:

>100_bases
GAAGTTGAAGGAGTTCCTGTGGCAAAAAGAGGGAGGATTCCGGGAATAATAGAAAACAAGAAGTTAAAAAAGATTAAGTG
ATTTCCGGAGATGAGTGTTA

Downstream 100 bases:

>100_bases
CAGGTGAGATAAAAGATTATATAATTTATGACGTACAAGGAAGTTTCGCGGCGGTGGCGGAACTGGCAGACGCGTACGTT
TGAGGGGCGTATGGGCTAAT

Product: ABC-type dipeptide/oligopeptide/nickel transport systems, periplasmic components

Products: ADP; phosphate; dipeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 528; Mature: 528

Protein sequence:

>528_residues
MKRFLAFVFIFAFLLTGCKINSPDITAIPEKKESKKEASQEEIQEGGTLRVNITSFDTLNPFLNDNEMVRQLLNLTLEPL
VTLDKSLKPIPLLAYKWEVNGLNIKFYLRKNIKWQDGESFTARDVKFTMDSFLDKGSKSPYKDLIKAHISSYREIGDYEF
EVSLNGPVANPLALFIFPIVAEHQYKDKGDIVNPLLVPIGTGPYKIVSYGQGNSVVLKRNPHYWGEKPHLEEINFKIVSD
ENAMITSFLSKEADFTFIKDVDWDKYRENSSINIYKYTLQRYVFLAPNLKKQVFSDVAVRKAIAYGIDVDRILREVYFKH
GLKSNVPIRPDSWLYSPKIASVYSIKEADKVLQDAGWLKANGIGTKGGYQLKFNLLVNSDNPYLLKVAQIIKTNLKEIGI
DINVVPASWDDLLKSVFSGNFDLALMEWNLNYNQDISSMFMTRGKDNFMGYSNPSLDDLFNKVFSETDEKSLRNNYYKVG
EFLAEEQPVIGLFYLEGAVMAYGNVKGIDPTSFNVFNNISSWYLKKQY

Sequences:

>Translated_528_residues
MKRFLAFVFIFAFLLTGCKINSPDITAIPEKKESKKEASQEEIQEGGTLRVNITSFDTLNPFLNDNEMVRQLLNLTLEPL
VTLDKSLKPIPLLAYKWEVNGLNIKFYLRKNIKWQDGESFTARDVKFTMDSFLDKGSKSPYKDLIKAHISSYREIGDYEF
EVSLNGPVANPLALFIFPIVAEHQYKDKGDIVNPLLVPIGTGPYKIVSYGQGNSVVLKRNPHYWGEKPHLEEINFKIVSD
ENAMITSFLSKEADFTFIKDVDWDKYRENSSINIYKYTLQRYVFLAPNLKKQVFSDVAVRKAIAYGIDVDRILREVYFKH
GLKSNVPIRPDSWLYSPKIASVYSIKEADKVLQDAGWLKANGIGTKGGYQLKFNLLVNSDNPYLLKVAQIIKTNLKEIGI
DINVVPASWDDLLKSVFSGNFDLALMEWNLNYNQDISSMFMTRGKDNFMGYSNPSLDDLFNKVFSETDEKSLRNNYYKVG
EFLAEEQPVIGLFYLEGAVMAYGNVKGIDPTSFNVFNNISSWYLKKQY
>Mature_528_residues
MKRFLAFVFIFAFLLTGCKINSPDITAIPEKKESKKEASQEEIQEGGTLRVNITSFDTLNPFLNDNEMVRQLLNLTLEPL
VTLDKSLKPIPLLAYKWEVNGLNIKFYLRKNIKWQDGESFTARDVKFTMDSFLDKGSKSPYKDLIKAHISSYREIGDYEF
EVSLNGPVANPLALFIFPIVAEHQYKDKGDIVNPLLVPIGTGPYKIVSYGQGNSVVLKRNPHYWGEKPHLEEINFKIVSD
ENAMITSFLSKEADFTFIKDVDWDKYRENSSINIYKYTLQRYVFLAPNLKKQVFSDVAVRKAIAYGIDVDRILREVYFKH
GLKSNVPIRPDSWLYSPKIASVYSIKEADKVLQDAGWLKANGIGTKGGYQLKFNLLVNSDNPYLLKVAQIIKTNLKEIGI
DINVVPASWDDLLKSVFSGNFDLALMEWNLNYNQDISSMFMTRGKDNFMGYSNPSLDDLFNKVFSETDEKSLRNNYYKVG
EFLAEEQPVIGLFYLEGAVMAYGNVKGIDPTSFNVFNNISSWYLKKQY

Specific function: This protein is a component of an oligopeptide permease, a binding protein-dependent transport system. This APP system can completely substitute for the OPP system in both sporulation and genetic competence. AppA can bind and transport tetra- and pentapep

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789966, Length=469, Percent_Identity=25.5863539445629, Blast_Score=129, Evalue=3e-31,
Organism=Escherichia coli, GI1787495, Length=555, Percent_Identity=23.7837837837838, Blast_Score=113, Evalue=3e-26,
Organism=Escherichia coli, GI1789887, Length=478, Percent_Identity=24.2677824267782, Blast_Score=112, Evalue=6e-26,
Organism=Escherichia coli, GI1787551, Length=529, Percent_Identity=23.4404536862004, Blast_Score=104, Evalue=2e-23,
Organism=Escherichia coli, GI87081878, Length=460, Percent_Identity=23.9130434782609, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1787052, Length=471, Percent_Identity=22.9299363057325, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1787762, Length=438, Percent_Identity=21.9178082191781, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1789397, Length=472, Percent_Identity=23.3050847457627, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI87082063, Length=419, Percent_Identity=21.7183770883055, Blast_Score=80, Evalue=2e-16,

Paralogues:

None

Copy number: 660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2980 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 60432; Mature: 60432

Theoretical pI: Translated: 7.64; Mature: 7.64

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRFLAFVFIFAFLLTGCKINSPDITAIPEKKESKKEASQEEIQEGGTLRVNITSFDTLN
CHHHHHHHHHHHHHHCCCCCCCCCCEECCCCHHHHHHHHHHHHHCCCEEEEEEECCCCCC
PFLNDNEMVRQLLNLTLEPLVTLDKSLKPIPLLAYKWEVNGLNIKFYLRKNIKWQDGESF
CCCCCHHHHHHHHHCHHHHHHHHHCCCCCCEEEEEEEEECCEEEEEEEEECCCCCCCCCC
TARDVKFTMDSFLDKGSKSPYKDLIKAHISSYREIGDYEFEVSLNGPVANPLALFIFPIV
EECHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHH
AEHQYKDKGDIVNPLLVPIGTGPYKIVSYGQGNSVVLKRNPHYWGEKPHLEEINFKIVSD
HCCCCCCCCCCCCCEEEEECCCCEEEEEECCCCEEEEECCCCCCCCCCCCCCCCEEEECC
ENAMITSFLSKEADFTFIKDVDWDKYRENSSINIYKYTLQRYVFLAPNLKKQVFSDVAVR
CCHHHHHHHHCCCCEEEEECCCHHHHCCCCCEEEEEEEEEEEEEECCCHHHHHHHHHHHH
KAIAYGIDVDRILREVYFKHGLKSNVPIRPDSWLYSPKIASVYSIKEADKVLQDAGWLKA
HHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEE
NGIGTKGGYQLKFNLLVNSDNPYLLKVAQIIKTNLKEIGIDINVVPASWDDLLKSVFSGN
CCCCCCCCEEEEEEEEEECCCCEEHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHCCC
FDLALMEWNLNYNQDISSMFMTRGKDNFMGYSNPSLDDLFNKVFSETDEKSLRNNYYKVG
CEEEEEEECCCCCCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHH
EFLAEEQPVIGLFYLEGAVMAYGNVKGIDPTSFNVFNNISSWYLKKQY
HHHHCCCCEEEEEEECCEEEEECCCCCCCCCCHHHHHCCHHHEEEECC
>Mature Secondary Structure
MKRFLAFVFIFAFLLTGCKINSPDITAIPEKKESKKEASQEEIQEGGTLRVNITSFDTLN
CHHHHHHHHHHHHHHCCCCCCCCCCEECCCCHHHHHHHHHHHHHCCCEEEEEEECCCCCC
PFLNDNEMVRQLLNLTLEPLVTLDKSLKPIPLLAYKWEVNGLNIKFYLRKNIKWQDGESF
CCCCCHHHHHHHHHCHHHHHHHHHCCCCCCEEEEEEEEECCEEEEEEEEECCCCCCCCCC
TARDVKFTMDSFLDKGSKSPYKDLIKAHISSYREIGDYEFEVSLNGPVANPLALFIFPIV
EECHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHH
AEHQYKDKGDIVNPLLVPIGTGPYKIVSYGQGNSVVLKRNPHYWGEKPHLEEINFKIVSD
HCCCCCCCCCCCCCEEEEECCCCEEEEEECCCCEEEEECCCCCCCCCCCCCCCCEEEECC
ENAMITSFLSKEADFTFIKDVDWDKYRENSSINIYKYTLQRYVFLAPNLKKQVFSDVAVR
CCHHHHHHHHCCCCEEEEECCCHHHHCCCCCEEEEEEEEEEEEEECCCHHHHHHHHHHHH
KAIAYGIDVDRILREVYFKHGLKSNVPIRPDSWLYSPKIASVYSIKEADKVLQDAGWLKA
HHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEE
NGIGTKGGYQLKFNLLVNSDNPYLLKVAQIIKTNLKEIGIDINVVPASWDDLLKSVFSGN
CCCCCCCCEEEEEEEEEECCCCEEHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHCCC
FDLALMEWNLNYNQDISSMFMTRGKDNFMGYSNPSLDDLFNKVFSETDEKSLRNNYYKVG
CEEEEEEECCCCCCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHH
EFLAEEQPVIGLFYLEGAVMAYGNVKGIDPTSFNVFNNISSWYLKKQY
HHHHCCCCEEEEEEECCEEEEECCCCCCCCCCHHHHHCCHHHEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; dipeptides [Periplasm]; H2O [C]

Specific reaction: ATP + dipeptides [Periplasm] + H2O = ADP + phosphate + dipeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7997159; 9384377 [H]