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The map label for this gene is MltE [H]

Identifier: 20807357

GI number: 20807357

Start: 885912

End: 886469

Strand: Direct

Name: MltE [H]

Synonym: TTE0876

Alternate gene names: 20807357

Gene position: 885912-886469 (Clockwise)

Preceding gene: 20807356

Following gene: 20807358

Centisome position: 32.94

GC content: 35.66

Gene sequence:

>558_bases
TTGATTAAGAGATGGGTACTTTTTCTACTAATAATCTTGGCTATTCTTTTGACGTTTGAAATGAATACTCACTATTTTTT
GAAGAAATTTTATCCTTTAAAGTACGAAGAATATGTAAATTCCTATTCCAAAGAATTTGGACTGGACCCTTATCTCGTTT
TTGCGATAATAAAAGTAGAGAGCAACTTCAATCCCTATGCGATATCGAACAGGAATGCCATAGGTTTAATGCAGATAATG
CCTGATACTGGAAGATGGATTGCCGCAAAACTGGGAATGAAAGATTTTAAGGAAGAAATCCTTTTTAATCCAGAGGTCAA
CATAAAAATGGGGGTGTGGTACCTTCATTATCTTCTCAAAAATTTTGATGGCAATCTAAAGTTAGCACTTGCGGCTTACA
ATGGTGGATGCGGAAATGTAGACCTTTGGCTTAAAGACAAGAGGTTTTCACAGGATGGAAGACAGCTTCACAGTATTCCA
TTTCCTGAGACGGATAGATACGTGAAGAAGGTGTTGGCAGTTTACCAAATGTATAAGTTTATATATACAAAAAAGTGA

Upstream 100 bases:

>100_bases
ATAAAGTACGCGGATTTTATAATAAATAACAGCAAAGATTTGAGGAAAACTGAAGAGCAAGTACGACTTTTATGGCAAAG
ATTTGATCGGAGGAGTTACT

Downstream 100 bases:

>100_bases
AGAAAGCTGGTATAATAGTAATCGTTGAAAGAAAAAAATATTGGGATAGGGAGGACAAAACAGTTGAAAAAGCTAAAATA
TTTAAAGGACTTAAAAGAAG

Product: lytic murein transglycosylase

Products: 1,6-Anhydrobond [C]

Alternate protein names: NA

Number of amino acids: Translated: 185; Mature: 185

Protein sequence:

>185_residues
MIKRWVLFLLIILAILLTFEMNTHYFLKKFYPLKYEEYVNSYSKEFGLDPYLVFAIIKVESNFNPYAISNRNAIGLMQIM
PDTGRWIAAKLGMKDFKEEILFNPEVNIKMGVWYLHYLLKNFDGNLKLALAAYNGGCGNVDLWLKDKRFSQDGRQLHSIP
FPETDRYVKKVLAVYQMYKFIYTKK

Sequences:

>Translated_185_residues
MIKRWVLFLLIILAILLTFEMNTHYFLKKFYPLKYEEYVNSYSKEFGLDPYLVFAIIKVESNFNPYAISNRNAIGLMQIM
PDTGRWIAAKLGMKDFKEEILFNPEVNIKMGVWYLHYLLKNFDGNLKLALAAYNGGCGNVDLWLKDKRFSQDGRQLHSIP
FPETDRYVKKVLAVYQMYKFIYTKK
>Mature_185_residues
MIKRWVLFLLIILAILLTFEMNTHYFLKKFYPLKYEEYVNSYSKEFGLDPYLVFAIIKVESNFNPYAISNRNAIGLMQIM
PDTGRWIAAKLGMKDFKEEILFNPEVNIKMGVWYLHYLLKNFDGNLKLALAAYNGGCGNVDLWLKDKRFSQDGRQLHSIP
FPETDRYVKKVLAVYQMYKFIYTKK

Specific function: Murein-Degrading Enzyme. Catalyzes The Cleavage Of The Glycosidic Bonds Between N-Acetylmuramic Acid And N- Acetylglucosamine Residues In Peptidoglycan. May Play A Role In Recycling Of Muropeptides During Cell Elongation And/Or Cell Division. [C]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Periplasmic Protein. Tightly Associated With The Murein Sacculus [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transglycosylase slt family [H]

Homologues:

Organism=Escherichia coli, GI87082441, Length=161, Percent_Identity=34.7826086956522, Blast_Score=88, Evalue=3e-19,
Organism=Escherichia coli, GI87082191, Length=189, Percent_Identity=30.1587301587302, Blast_Score=73, Evalue=1e-14,
Organism=Escherichia coli, GI1786405, Length=127, Percent_Identity=35.4330708661417, Blast_Score=66, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008258
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 21898; Mature: 21898

Theoretical pI: Translated: 9.77; Mature: 9.77

Prosite motif: PS00922 TRANSGLYCOSYLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKRWVLFLLIILAILLTFEMNTHYFLKKFYPLKYEEYVNSYSKEFGLDPYLVFAIIKVE
CHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHHHHCCCHHHEEEEEEEE
SNFNPYAISNRNAIGLMQIMPDTGRWIAAKLGMKDFKEEILFNPEVNIKMGVWYLHYLLK
CCCCCEEECCCCCEEEEEECCCCCCEEEECCCHHHHHHHHCCCCCCCEEHHHHHHHHHHH
NFDGNLKLALAAYNGGCGNVDLWLKDKRFSQDGRQLHSIPFPETDRYVKKVLAVYQMYKF
CCCCCEEEEEEEECCCCCCEEEEEECCCCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHH
IYTKK
HHCCC
>Mature Secondary Structure
MIKRWVLFLLIILAILLTFEMNTHYFLKKFYPLKYEEYVNSYSKEFGLDPYLVFAIIKVE
CHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHHHHCCCHHHEEEEEEEE
SNFNPYAISNRNAIGLMQIMPDTGRWIAAKLGMKDFKEEILFNPEVNIKMGVWYLHYLLK
CCCCCEEECCCCCEEEEEECCCCCCEEEECCCHHHHHHHHCCCCCCCEEHHHHHHHHHHH
NFDGNLKLALAAYNGGCGNVDLWLKDKRFSQDGRQLHSIPFPETDRYVKKVLAVYQMYKF
CCCCCEEEEEEEECCCCCCEEEEEECCCCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHH
IYTKK
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]