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The map label for this gene is PspF4 [H]

Identifier: 20807307

GI number: 20807307

Start: 833324

End: 834640

Strand: Direct

Name: PspF4 [H]

Synonym: TTE0825

Alternate gene names: 20807307

Gene position: 833324-834640 (Clockwise)

Preceding gene: 20807303

Following gene: 20807309

Centisome position: 30.98

GC content: 33.26

Gene sequence:

>1317_bases
ATGGTGATAAGTATGTGCAATAAGGATAAGGTTTACAAAGTTGTGGTAGAAAAAAGTAAAAACTTCAAATTGGAAGATTT
CGTTTTAAGTAAAAGCGGTGCTGCTACTACAAATGAGATAGCAAGAATCCTTAACCTCGCGCGTTCTAATGTATCAGCTA
TTTTGAATGAACTTGTAAGAGAAGAAAAAATTATTAAAATAGTTTCTAAACCGGTCTATTACGTTGAAAAAGAAATTTTT
ATTCAAGCTTTTGGGAAAAGAATTAAATATGAAATCAACTTTAGTGAATTGATAAATGTTGTGAGTGAAATTAAAGAGGT
AGGAGTTGAAGCTTTTGGTTTTGATAATATCATTGGATATAACGGTAGTTTGAAGGAACAAATAGAGCAGGCAAAAGCCG
CGGTAGTTTATCCGCCTAACGGATTACATACTCTCATTATTGGACCACCCGGTTCGGGCAAAACTTATTTGGCGGAAAGT
ATGTATAAGTTTGGGCGTCGAAAAGGATTGAAAGGGTCTTTTGAGGTTTTAAACTGTGCAGATTATTACCACAATCCTCA
GCTTCTCCTTTCTCACCTGTTTGGTCATGCAAAAGGTGCTTATACAGGAGCTTTTGTTGAAAGAATAGGTTTGGTAGAAA
AAGCGAATAATGGGATACTATTTTTAGATGAGGTTCATCGCCTTCCTCCGGAAGGTCAAGAAATGCTTTTTTATCTTATA
GATAAAGGAATGTATAAAAGGCTGGGAGACACAGAGGAAAGAAAGGTAAATGTAATGATTATTGCAGCTACTACAGAAAA
AATTGATTCGATACTCTTGAGGACTTTTATAAGAAGAATACCTGTTATAATTCAACTTCCTTCATTTGATGAAAGACCGG
TCGAAGAGAAGATGGAAATAATTGTTTATCTCTTAAGGGAAGAATCCAAGAGATTAGGTGTGGATATTATTATTTCTTCC
CATCTACTTGCTTTACTTTCTCTCTTTAAGTATGAAGGTAACATTGGGGAGTTGAAGTCTCTCATACAGCTCATCTGTTC
CAAGGCTTTTCTAGCTAATTTTCAGATTAACAGAAAATTTATAAAATTAGATTCTGCTTTATTGCCTTCCAAATATTATA
AAAAAGAAATTATTAATACAGAAACGTTTGATGAATTTTTTATCATAACTCCTTTTGGGAAAAGGAGCATTGACAGATAC
GATTTATACGAGTTTATAGCCGGGAAATATCATGAATTAAAATCAAAGGGCTATGAAGAAAAAACAATTAGAGATAAAAT
AATTGATTACGTTAGTAACTTCTTAGATGATGTATAG

Upstream 100 bases:

>100_bases
CGAATTATGTTGAAAAAATCTTTTTATGACAATGAAGGAAAATTTGGGTCTATATCTAAATAATAAATATATGGAAGGAA
AAGAATGTATAGCAGTGAAA

Downstream 100 bases:

>100_bases
CTGCTTCTTTTATAGCAAGCTTTTACTTTTTGTTTTTTTATCCCTTTTTACTAAAATTCCTGCAGCGAGGGCAGTTAGAG
GTATACAGCAACTAATCCCG

Product: transcriptional regulator

Products: NA

Alternate protein names: Putative phosphotransferase EIIA component; Putative PTS system EIIA component [H]

Number of amino acids: Translated: 438; Mature: 438

Protein sequence:

>438_residues
MVISMCNKDKVYKVVVEKSKNFKLEDFVLSKSGAATTNEIARILNLARSNVSAILNELVREEKIIKIVSKPVYYVEKEIF
IQAFGKRIKYEINFSELINVVSEIKEVGVEAFGFDNIIGYNGSLKEQIEQAKAAVVYPPNGLHTLIIGPPGSGKTYLAES
MYKFGRRKGLKGSFEVLNCADYYHNPQLLLSHLFGHAKGAYTGAFVERIGLVEKANNGILFLDEVHRLPPEGQEMLFYLI
DKGMYKRLGDTEERKVNVMIIAATTEKIDSILLRTFIRRIPVIIQLPSFDERPVEEKMEIIVYLLREESKRLGVDIIISS
HLLALLSLFKYEGNIGELKSLIQLICSKAFLANFQINRKFIKLDSALLPSKYYKKEIINTETFDEFFIITPFGKRSIDRY
DLYEFIAGKYHELKSKGYEEKTIRDKIIDYVSNFLDDV

Sequences:

>Translated_438_residues
MVISMCNKDKVYKVVVEKSKNFKLEDFVLSKSGAATTNEIARILNLARSNVSAILNELVREEKIIKIVSKPVYYVEKEIF
IQAFGKRIKYEINFSELINVVSEIKEVGVEAFGFDNIIGYNGSLKEQIEQAKAAVVYPPNGLHTLIIGPPGSGKTYLAES
MYKFGRRKGLKGSFEVLNCADYYHNPQLLLSHLFGHAKGAYTGAFVERIGLVEKANNGILFLDEVHRLPPEGQEMLFYLI
DKGMYKRLGDTEERKVNVMIIAATTEKIDSILLRTFIRRIPVIIQLPSFDERPVEEKMEIIVYLLREESKRLGVDIIISS
HLLALLSLFKYEGNIGELKSLIQLICSKAFLANFQINRKFIKLDSALLPSKYYKKEIINTETFDEFFIITPFGKRSIDRY
DLYEFIAGKYHELKSKGYEEKTIRDKIIDYVSNFLDDV
>Mature_438_residues
MVISMCNKDKVYKVVVEKSKNFKLEDFVLSKSGAATTNEIARILNLARSNVSAILNELVREEKIIKIVSKPVYYVEKEIF
IQAFGKRIKYEINFSELINVVSEIKEVGVEAFGFDNIIGYNGSLKEQIEQAKAAVVYPPNGLHTLIIGPPGSGKTYLAES
MYKFGRRKGLKGSFEVLNCADYYHNPQLLLSHLFGHAKGAYTGAFVERIGLVEKANNGILFLDEVHRLPPEGQEMLFYLI
DKGMYKRLGDTEERKVNVMIIAATTEKIDSILLRTFIRRIPVIIQLPSFDERPVEEKMEIIVYLLREESKRLGVDIIISS
HLLALLSLFKYEGNIGELKSLIQLICSKAFLANFQINRKFIKLDSALLPSKYYKKEIINTETFDEFFIITPFGKRSIDRY
DLYEFIAGKYHELKSKGYEEKTIRDKIIDYVSNFLDDV

Specific function: Involved in positive regulation of the levanase operon which comprises the levDEFG genes for a fructose PTS system, and sacA for levanase [H]

COG id: COG1221

COG function: function code KT; Transcriptional regulators containing an AAA-type ATPase domain and a DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=207, Percent_Identity=35.2657004830918, Blast_Score=98, Evalue=9e-22,
Organism=Escherichia coli, GI1789233, Length=240, Percent_Identity=32.5, Blast_Score=96, Evalue=6e-21,
Organism=Escherichia coli, GI87081872, Length=237, Percent_Identity=31.2236286919831, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1789087, Length=206, Percent_Identity=33.0097087378641, Blast_Score=92, Evalue=8e-20,
Organism=Escherichia coli, GI1790299, Length=212, Percent_Identity=32.0754716981132, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI87082117, Length=279, Percent_Identity=27.9569892473118, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1788905, Length=223, Percent_Identity=30.0448430493274, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI87082152, Length=227, Percent_Identity=30.3964757709251, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI1789828, Length=148, Percent_Identity=33.7837837837838, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1787583, Length=238, Percent_Identity=27.3109243697479, Blast_Score=79, Evalue=8e-16,
Organism=Escherichia coli, GI1790437, Length=249, Percent_Identity=29.718875502008, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1786524, Length=239, Percent_Identity=27.1966527196653, Blast_Score=69, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011608
- InterPro:   IPR004701
- InterPro:   IPR002078
- InterPro:   IPR001550 [H]

Pfam domain/function: PF03610 EIIA-man; PF00874 PRD; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 50142; Mature: 50142

Theoretical pI: Translated: 8.79; Mature: 8.79

Prosite motif: PS00690 DEAH_ATP_HELICASE ; PS00676 SIGMA54_INTERACT_2 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVISMCNKDKVYKVVVEKSKNFKLEDFVLSKSGAATTNEIARILNLARSNVSAILNELVR
CEEEECCCCCEEEEEEECCCCCEEHHEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
EEKIIKIVSKPVYYVEKEIFIQAFGKRIKYEINFSELINVVSEIKEVGVEAFGFDNIIGY
HHHHHHHHHCCHHHHHHHHHHHHHCCCEEEEECHHHHHHHHHHHHHCCCHHCCCCCEECC
NGSLKEQIEQAKAAVVYPPNGLHTLIIGPPGSGKTYLAESMYKFGRRKGLKGSFEVLNCA
CCCHHHHHHHCCEEEEECCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCHHHEEHH
DYYHNPQLLLSHLFGHAKGAYTGAFVERIGLVEKANNGILFLDEVHRLPPEGQEMLFYLI
HHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCEEEEEHHHCCCCCHHHHHHHHH
DKGMYKRLGDTEERKVNVMIIAATTEKIDSILLRTFIRRIPVIIQLPSFDERPVEEKMEI
HHHHHHHCCCCCCCEEEEEEEEECHHHHHHHHHHHHHHHCCEEEECCCCCCCCHHHHHHH
IVYLLREESKRLGVDIIISSHLLALLSLFKYEGNIGELKSLIQLICSKAFLANFQINRKF
HHHHHHHHHHHCCCCEEHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCHHH
IKLDSALLPSKYYKKEIINTETFDEFFIITPFGKRSIDRYDLYEFIAGKYHELKSKGYEE
EEHHHHHCCHHHHHHHHCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCHH
KTIRDKIIDYVSNFLDDV
HHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MVISMCNKDKVYKVVVEKSKNFKLEDFVLSKSGAATTNEIARILNLARSNVSAILNELVR
CEEEECCCCCEEEEEEECCCCCEEHHEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
EEKIIKIVSKPVYYVEKEIFIQAFGKRIKYEINFSELINVVSEIKEVGVEAFGFDNIIGY
HHHHHHHHHCCHHHHHHHHHHHHHCCCEEEEECHHHHHHHHHHHHHCCCHHCCCCCEECC
NGSLKEQIEQAKAAVVYPPNGLHTLIIGPPGSGKTYLAESMYKFGRRKGLKGSFEVLNCA
CCCHHHHHHHCCEEEEECCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCHHHEEHH
DYYHNPQLLLSHLFGHAKGAYTGAFVERIGLVEKANNGILFLDEVHRLPPEGQEMLFYLI
HHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCEEEEEHHHCCCCCHHHHHHHHH
DKGMYKRLGDTEERKVNVMIIAATTEKIDSILLRTFIRRIPVIIQLPSFDERPVEEKMEI
HHHHHHHCCCCCCCEEEEEEEEECHHHHHHHHHHHHHHHCCEEEECCCCCCCCHHHHHHH
IVYLLREESKRLGVDIIISSHLLALLSLFKYEGNIGELKSLIQLICSKAFLANFQINRKF
HHHHHHHHHHHCCCCEEHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCHHH
IKLDSALLPSKYYKKEIINTETFDEFFIITPFGKRSIDRYDLYEFIAGKYHELKSKGYEE
EEHHHHHCCHHHHHHHHCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCHH
KTIRDKIIDYVSNFLDDV
HHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1900939; 9141695; 9384377 [H]