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The map label for this gene is PspF3 [H]

Identifier: 20807204

GI number: 20807204

Start: 719616

End: 721052

Strand: Direct

Name: PspF3 [H]

Synonym: TTE0717

Alternate gene names: 20807204

Gene position: 719616-721052 (Clockwise)

Preceding gene: 20807203

Following gene: 20807205

Centisome position: 26.76

GC content: 35.98

Gene sequence:

>1437_bases
ATGGATTTGAGGTTAAAAGGATTACCTCAGGAATTTTACCAAAAAGCGCTGGAGATATTATTAGAAAACATTGATTGGGG
TGTACAAATCACTGATAAAGAGGGTTATACAATTTATTATAATAAAGCTTCTTCTCAAATTGATGGAATTCCTATAGATG
AAGCGATTGGAAAGCACGTTTTAGCTATGTTTCCATCCCTTTCTAATAGAGAAAGTACAATTTTAAAAGTTTTAAGGACA
GGGGAACCTGTTGTCGGACATGAGCAGGAAATTACAAATTGCTATGGACAAAAAGTCAGCCTTTTTACTATTACTCTCCC
CATTGTAATCGATGGAGAAATATGCGGGGCTATTGATATTTCTAAAAACCTCTCTGAAATAAAAAATCTTATGACAAAAG
TGATTGATTTGCGAGAAGAAGTGCGAAATAACAGAAAGAAAAGTGCTGTAAAAGAAAACGAGGAAGCTTATTATACTTTT
GATGATATTATTGGAGAATCCAAAGTAATTACAGAACTAAAGTGCAAGGCTCAAAAAATTGCAAAAAGCAATTCGCCAGT
GTTTGTATATGGTGAGACAGGTACGGGCAAAGAGCTTTTAGTACAGGCAATTCATAATGCTAGTCCAAGGAAGAATGGAC
CTTTTATTGCTCAAAATTGCGCAGCCCTTCCTGAAAATTTGATGGAGTCAATACTTTTTGGAACAGTAAAGGGAAGCTTT
ACTGGTGCAGAAAATCGCCCTGGTCTGGTAGAGCTGGCTGACGGAGGGACATTATTTTTGGATGAGATTACTTCTTTAAG
TTTTGACCTTCAGGCAAAGCTTTTACGCTTTTTGCAGGAAGGGTATATAAGGAGAATAGGAGATAGTAAGGTTCGAAAAG
TAGATGTGAGAGTTATTGCTGCTTCTAACATTTCTCCTTATGAAGCTTTAGAGAAGAAAATTTTAAGACCTGACCTTTTT
TATCGATTAAGTACCATTGTCTTGTATGTGCCACCTCTTAGGGAGAGAAAAAGTGATATTCCTCTTTTAGTTAAGCACTT
TATCAAGGAATTTAATTGGCAATTGGGGAAGGATATAAAAGGGATAAGCGAAGAGGTGGAAAGAGCGCTGATGAGTTTGT
CCTGGCCGGGAAATGTGCGGGAATTAAAGGGAGTTATAGAGTATGCGGCTAATTTTTGCGAAGGTGAAATAATTCAGTTG
CATGACCTTCCTGACCACATTTTAAGAAATGCCTTTATAGAGGGAGAAAAAGAGATAGCAGTAGATACTATCGGGATAGA
AGGGGATCTCAGAAGCTTTCTAGCAAAAGTTGAATATGAGCTTATTACTAAAACTTTAAAAAAGACAAATGGGAATATAA
GTAGAGCAGCAGAAATTTTGGGTATTCCGAGACAGACTTTACAATACAAAATAAAAATGTTAAAAATAAATATTTAA

Upstream 100 bases:

>100_bases
GCATAAATAGCGCTTTTTTGTTAGAAATGAAGAAATACTTACATGTCGAAAATATTTTCGGTGTTTTTGCTTTTAATTTT
ACTCTCAAAGGGGGAGTAAG

Downstream 100 bases:

>100_bases
TTTTGCGCCTAATGCTTAATTTTCAGCATAAGATGGGTTTTTATCAGAACGAGCTGTGAATCTTTTATAGGAAAAGGCCG
AAAAATGGCCTTTTTTCTTT

Product: transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 478; Mature: 478

Protein sequence:

>478_residues
MDLRLKGLPQEFYQKALEILLENIDWGVQITDKEGYTIYYNKASSQIDGIPIDEAIGKHVLAMFPSLSNRESTILKVLRT
GEPVVGHEQEITNCYGQKVSLFTITLPIVIDGEICGAIDISKNLSEIKNLMTKVIDLREEVRNNRKKSAVKENEEAYYTF
DDIIGESKVITELKCKAQKIAKSNSPVFVYGETGTGKELLVQAIHNASPRKNGPFIAQNCAALPENLMESILFGTVKGSF
TGAENRPGLVELADGGTLFLDEITSLSFDLQAKLLRFLQEGYIRRIGDSKVRKVDVRVIAASNISPYEALEKKILRPDLF
YRLSTIVLYVPPLRERKSDIPLLVKHFIKEFNWQLGKDIKGISEEVERALMSLSWPGNVRELKGVIEYAANFCEGEIIQL
HDLPDHILRNAFIEGEKEIAVDTIGIEGDLRSFLAKVEYELITKTLKKTNGNISRAAEILGIPRQTLQYKIKMLKINI

Sequences:

>Translated_478_residues
MDLRLKGLPQEFYQKALEILLENIDWGVQITDKEGYTIYYNKASSQIDGIPIDEAIGKHVLAMFPSLSNRESTILKVLRT
GEPVVGHEQEITNCYGQKVSLFTITLPIVIDGEICGAIDISKNLSEIKNLMTKVIDLREEVRNNRKKSAVKENEEAYYTF
DDIIGESKVITELKCKAQKIAKSNSPVFVYGETGTGKELLVQAIHNASPRKNGPFIAQNCAALPENLMESILFGTVKGSF
TGAENRPGLVELADGGTLFLDEITSLSFDLQAKLLRFLQEGYIRRIGDSKVRKVDVRVIAASNISPYEALEKKILRPDLF
YRLSTIVLYVPPLRERKSDIPLLVKHFIKEFNWQLGKDIKGISEEVERALMSLSWPGNVRELKGVIEYAANFCEGEIIQL
HDLPDHILRNAFIEGEKEIAVDTIGIEGDLRSFLAKVEYELITKTLKKTNGNISRAAEILGIPRQTLQYKIKMLKINI
>Mature_478_residues
MDLRLKGLPQEFYQKALEILLENIDWGVQITDKEGYTIYYNKASSQIDGIPIDEAIGKHVLAMFPSLSNRESTILKVLRT
GEPVVGHEQEITNCYGQKVSLFTITLPIVIDGEICGAIDISKNLSEIKNLMTKVIDLREEVRNNRKKSAVKENEEAYYTF
DDIIGESKVITELKCKAQKIAKSNSPVFVYGETGTGKELLVQAIHNASPRKNGPFIAQNCAALPENLMESILFGTVKGSF
TGAENRPGLVELADGGTLFLDEITSLSFDLQAKLLRFLQEGYIRRIGDSKVRKVDVRVIAASNISPYEALEKKILRPDLF
YRLSTIVLYVPPLRERKSDIPLLVKHFIKEFNWQLGKDIKGISEEVERALMSLSWPGNVRELKGVIEYAANFCEGEIIQL
HDLPDHILRNAFIEGEKEIAVDTIGIEGDLRSFLAKVEYELITKTLKKTNGNISRAAEILGIPRQTLQYKIKMLKINI

Specific function: Positive regulator of arginine catabolism. Controls the transcription of the two operons rocABC and rocDEF and probably acts by binding to the corresponding upstream activating sequences [H]

COG id: COG3829

COG function: function code KT; Transcriptional regulator containing PAS, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=365, Percent_Identity=41.0958904109589, Blast_Score=249, Evalue=4e-67,
Organism=Escherichia coli, GI1789087, Length=424, Percent_Identity=37.5, Blast_Score=248, Evalue=6e-67,
Organism=Escherichia coli, GI87082117, Length=332, Percent_Identity=42.7710843373494, Blast_Score=248, Evalue=7e-67,
Organism=Escherichia coli, GI1790299, Length=330, Percent_Identity=39.0909090909091, Blast_Score=236, Evalue=3e-63,
Organism=Escherichia coli, GI1789233, Length=469, Percent_Identity=34.3283582089552, Blast_Score=231, Evalue=9e-62,
Organism=Escherichia coli, GI1790437, Length=310, Percent_Identity=40.3225806451613, Blast_Score=223, Evalue=2e-59,
Organism=Escherichia coli, GI1788905, Length=376, Percent_Identity=36.7021276595745, Blast_Score=219, Evalue=4e-58,
Organism=Escherichia coli, GI87082152, Length=228, Percent_Identity=50.8771929824561, Blast_Score=209, Evalue=4e-55,
Organism=Escherichia coli, GI87081872, Length=332, Percent_Identity=37.9518072289157, Blast_Score=203, Evalue=2e-53,
Organism=Escherichia coli, GI1787583, Length=345, Percent_Identity=33.9130434782609, Blast_Score=193, Evalue=2e-50,
Organism=Escherichia coli, GI1786524, Length=365, Percent_Identity=34.2465753424658, Blast_Score=189, Evalue=3e-49,
Organism=Escherichia coli, GI87081858, Length=470, Percent_Identity=28.7234042553192, Blast_Score=160, Evalue=1e-40,
Organism=Escherichia coli, GI1789828, Length=301, Percent_Identity=29.2358803986711, Blast_Score=121, Evalue=1e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR000014
- InterPro:   IPR013656
- InterPro:   IPR002078 [H]

Pfam domain/function: PF02954 HTH_8; PF08448 PAS_4; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 53759; Mature: 53759

Theoretical pI: Translated: 6.54; Mature: 6.54

Prosite motif: PS50112 PAS ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLRLKGLPQEFYQKALEILLENIDWGVQITDKEGYTIYYNKASSQIDGIPIDEAIGKHV
CCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCEEEEEECCCCCCCCCCHHHHHHHHH
LAMFPSLSNRESTILKVLRTGEPVVGHEQEITNCYGQKVSLFTITLPIVIDGEICGAIDI
HHHCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHCCEEEEEEEEEEEEECCCCCCEEEC
SKNLSEIKNLMTKVIDLREEVRNNRKKSAVKENEEAYYTFDDIIGESKVITELKCKAQKI
CCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCEEEHHHHCCCCHHHHHHHHHHHHH
AKSNSPVFVYGETGTGKELLVQAIHNASPRKNGPFIAQNCAALPENLMESILFGTVKGSF
HCCCCCEEEEECCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCC
TGAENRPGLVELADGGTLFLDEITSLSFDLQAKLLRFLQEGYIRRIGDSKVRKVDVRVIA
CCCCCCCCEEEECCCCEEEEHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEE
ASNISPYEALEKKILRPDLFYRLSTIVLYVPPLRERKSDIPLLVKHFIKEFNWQLGKDIK
ECCCCHHHHHHHHHCCHHHHHHHHHHHEECCCHHCCCCCCHHHHHHHHHHHCCHHCCCHH
GISEEVERALMSLSWPGNVRELKGVIEYAANFCEGEIIQLHDLPDHILRNAFIEGEKEIA
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHCCCCCEEE
VDTIGIEGDLRSFLAKVEYELITKTLKKTNGNISRAAEILGIPRQTLQYKIKMLKINI
EEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHEEEEEEECC
>Mature Secondary Structure
MDLRLKGLPQEFYQKALEILLENIDWGVQITDKEGYTIYYNKASSQIDGIPIDEAIGKHV
CCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCEEEEEECCCCCCCCCCHHHHHHHHH
LAMFPSLSNRESTILKVLRTGEPVVGHEQEITNCYGQKVSLFTITLPIVIDGEICGAIDI
HHHCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHCCEEEEEEEEEEEEECCCCCCEEEC
SKNLSEIKNLMTKVIDLREEVRNNRKKSAVKENEEAYYTFDDIIGESKVITELKCKAQKI
CCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCEEEHHHHCCCCHHHHHHHHHHHHH
AKSNSPVFVYGETGTGKELLVQAIHNASPRKNGPFIAQNCAALPENLMESILFGTVKGSF
HCCCCCEEEEECCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCC
TGAENRPGLVELADGGTLFLDEITSLSFDLQAKLLRFLQEGYIRRIGDSKVRKVDVRVIA
CCCCCCCCEEEECCCCEEEEHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEE
ASNISPYEALEKKILRPDLFYRLSTIVLYVPPLRERKSDIPLLVKHFIKEFNWQLGKDIK
ECCCCHHHHHHHHHCCHHHHHHHHHHHEECCCHHCCCCCCHHHHHHHHHHHCCHHCCCHH
GISEEVERALMSLSWPGNVRELKGVIEYAANFCEGEIIQLHDLPDHILRNAFIEGEKEIA
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHCCCCCEEE
VDTIGIEGDLRSFLAKVEYELITKTLKKTNGNISRAAEILGIPRQTLQYKIKMLKINI
EEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8113162; 9384377 [H]