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The map label for this gene is MhpC [H]

Identifier: 20807053

GI number: 20807053

Start: 558818

End: 559657

Strand: Direct

Name: MhpC [H]

Synonym: TTE0552

Alternate gene names: 20807053

Gene position: 558818-559657 (Clockwise)

Preceding gene: 20807052

Following gene: 20807054

Centisome position: 20.78

GC content: 33.57

Gene sequence:

>840_bases
ATGCCTAAAATCACAGTTAATAATGTCAGTTTGTACTATGAAATACATGGAAATGGCTCACCTCTTGTATTAATAGAAGG
TCTAGGTTGTTCGAAATGGATGTGGTTTAAGCAAATAGATGAATTAAAAAAGCACTTTAAAGTTATTGTTTTTGACTTAA
GGGGTGTTGGTGATTCTGAGAAGCCAGATATGGAATATTCTATTAAACTACTCGCCGATGATACAGCGGCTTTAGTAGCA
GAACTTGGATTTAAAAAAGTTCATGTACTTGGGGTTTCTATGGGCGGTTATATAGCACAGGAGCTTGCCCTAGAATACCC
TGACCTTGTTGATAGATTGATACTATGTTCTACTCATTATGGAGGACCAAACATTGTGCCAATACCATTGTCTACTCTTA
ATATTATATTAAATGGCGCCGGTCTACGTAATGCATTAGAAAATTTGCGAATTGCTATGTCTTTAAATTTTAGTGATGAA
TATCTCTCTACCCATAAGGATGAGTTTGAACAAATCGTGAAATGGAAGTTTGAAAAGCCACAGCCATTTTATGCCTACAG
AAGACAACTTTATGCAGCTTTAACATTTGATGAAGAGGCAAGAATTCACTTAATTAAGCATCCTACTTTAATAATGGCAG
GTAAAGATGATAAAGTAGTGCCTTATGAAAATGCACTTTTATTACATTCAAAAATAGAAAATTCAGAAATAGAATTGTTT
AGTAATGCAGGACACATGTTTTTTATAGAAAAAGCAGAAGAAGTGAATCGGAAAATTATAGAGTTCTTAACAAAACCTAT
AGGAGGAGATAAAAAATGGGAAGAAAAAATGTTAATGTAG

Upstream 100 bases:

>100_bases
CCAATCAAAATGGAGAAGTTGTATTAGATGGTGTAGCCACAGTGCTCGCACCTAAGGAGTAAATAGAAAGGAATTATATT
AAAAAATAGGAGGCTAAGGG

Downstream 100 bases:

>100_bases
GAATTGTGGGAACAGGACTTTATATTCCTCAAACGTACATGACATCTGAAGATATAGCTAGAGAGACAGGAATACCCGAG
GAGATTATAAGAACCAAATT

Product: alpha/beta superfamily hydrolase/acyltransferase

Products: 2-oxopent-4-enoate; succinate [C]

Alternate protein names: PHA depolymerase; PHB depolymerase [H]

Number of amino acids: Translated: 279; Mature: 278

Protein sequence:

>279_residues
MPKITVNNVSLYYEIHGNGSPLVLIEGLGCSKWMWFKQIDELKKHFKVIVFDLRGVGDSEKPDMEYSIKLLADDTAALVA
ELGFKKVHVLGVSMGGYIAQELALEYPDLVDRLILCSTHYGGPNIVPIPLSTLNIILNGAGLRNALENLRIAMSLNFSDE
YLSTHKDEFEQIVKWKFEKPQPFYAYRRQLYAALTFDEEARIHLIKHPTLIMAGKDDKVVPYENALLLHSKIENSEIELF
SNAGHMFFIEKAEEVNRKIIEFLTKPIGGDKKWEEKMLM

Sequences:

>Translated_279_residues
MPKITVNNVSLYYEIHGNGSPLVLIEGLGCSKWMWFKQIDELKKHFKVIVFDLRGVGDSEKPDMEYSIKLLADDTAALVA
ELGFKKVHVLGVSMGGYIAQELALEYPDLVDRLILCSTHYGGPNIVPIPLSTLNIILNGAGLRNALENLRIAMSLNFSDE
YLSTHKDEFEQIVKWKFEKPQPFYAYRRQLYAALTFDEEARIHLIKHPTLIMAGKDDKVVPYENALLLHSKIENSEIELF
SNAGHMFFIEKAEEVNRKIIEFLTKPIGGDKKWEEKMLM
>Mature_278_residues
PKITVNNVSLYYEIHGNGSPLVLIEGLGCSKWMWFKQIDELKKHFKVIVFDLRGVGDSEKPDMEYSIKLLADDTAALVAE
LGFKKVHVLGVSMGGYIAQELALEYPDLVDRLILCSTHYGGPNIVPIPLSTLNIILNGAGLRNALENLRIAMSLNFSDEY
LSTHKDEFEQIVKWKFEKPQPFYAYRRQLYAALTFDEEARIHLIKHPTLIMAGKDDKVVPYENALLLHSKIENSEIELFS
NAGHMFFIEKAEEVNRKIIEFLTKPIGGDKKWEEKMLM

Specific function: PHA depolymerase is in fact a lipase [H]

COG id: COG0596

COG function: function code R; Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. Lipase family [H]

Homologues:

Organism=Escherichia coli, GI87081721, Length=278, Percent_Identity=27.3381294964029, Blast_Score=90, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR011942 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: 3.7.1.- [C]

Molecular weight: Translated: 31846; Mature: 31715

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: PS00120 LIPASE_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKITVNNVSLYYEIHGNGSPLVLIEGLGCSKWMWFKQIDELKKHFKVIVFDLRGVGDSE
CCCEEEEEEEEEEEEECCCCCEEEEECCCCCCHHHHHHHHHHHHHHEEEEEEEECCCCCC
KPDMEYSIKLLADDTAALVAELGFKKVHVLGVSMGGYIAQELALEYPDLVDRLILCSTHY
CCCCEEEEEEEECCHHHHHHHCCCCEEEEEEEEHHHHHHHHHHCCCHHHHHHHHHHCCCC
GGPNIVPIPLSTLNIILNGAGLRNALENLRIAMSLNFSDEYLSTHKDEFEQIVKWKFEKP
CCCEEEEECHHEEEEEEECCHHHHHHHCEEEEEEECCCHHHHHCCHHHHHHHHHCCCCCC
QPFYAYRRQLYAALTFDEEARIHLIKHPTLIMAGKDDKVVPYENALLLHSKIENSEIELF
CCHHHHHHHHHEEEEECCCCEEEEEECCEEEEECCCCCCCCCCCEEEEEECCCCCEEEEE
SNAGHMFFIEKAEEVNRKIIEFLTKPIGGDKKWEEKMLM
ECCCCEEEEECHHHHHHHHHHHHHCCCCCCCCHHHHCCC
>Mature Secondary Structure 
PKITVNNVSLYYEIHGNGSPLVLIEGLGCSKWMWFKQIDELKKHFKVIVFDLRGVGDSE
CCEEEEEEEEEEEEECCCCCEEEEECCCCCCHHHHHHHHHHHHHHEEEEEEEECCCCCC
KPDMEYSIKLLADDTAALVAELGFKKVHVLGVSMGGYIAQELALEYPDLVDRLILCSTHY
CCCCEEEEEEEECCHHHHHHHCCCCEEEEEEEEHHHHHHHHHHCCCHHHHHHHHHHCCCC
GGPNIVPIPLSTLNIILNGAGLRNALENLRIAMSLNFSDEYLSTHKDEFEQIVKWKFEKP
CCCEEEEECHHEEEEEEECCHHHHHHHCEEEEEEECCCHHHHHCCHHHHHHHHHCCCCCC
QPFYAYRRQLYAALTFDEEARIHLIKHPTLIMAGKDDKVVPYENALLLHSKIENSEIELF
CCHHHHHHHHHEEEEECCCCEEEEEECCEEEEECCCCCCCCCCCEEEEEECCCCCEEEEE
SNAGHMFFIEKAEEVNRKIIEFLTKPIGGDKKWEEKMLM
ECCCCEEEEECHHHHHHHHHHHHHCCCCCCCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-hydroxy-6-ketononadienedicarboxylate; H2O [C]

Specific reaction: 2-hydroxy-6-ketononadienedicarboxylate + H2O = 2-oxopent-4-enoate + succinate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1989978 [H]