Click here to switch to the map view.

The map label for this gene is PspF2 [H]

Identifier: 20807051

GI number: 20807051

Start: 556673

End: 558082

Strand: Direct

Name: PspF2 [H]

Synonym: TTE0550

Alternate gene names: 20807051

Gene position: 556673-558082 (Clockwise)

Preceding gene: 20807050

Following gene: 20807052

Centisome position: 20.7

GC content: 31.06

Gene sequence:

>1410_bases
ATGTCAAAAAATATTGATAAATCATTTTTGTTTAGTGAAGAAATGGAGTTTTTGTTCGAGAGTGTTTTTGACAAGTTACC
AATTGCTATTGATATACTTGATGCGGAAGGCAATATAAGAATGATAAATAAAACGTTTTTAGATTATTTGGGATTGGAAA
AGGACAAGGTCATTAACAAATATGTTTTAGACGTGGATCGAAATTCGAGGTTCCCTCTTGTTTTAAAAACAGGTCAAAAT
GAAATTGCTTACAGACATAAATTTGCCAATGGAAAGGAAGCTATTGTACATAGAATTGCAATAAAGGATGGAGATGAAGT
AATTGGTGGTTTTGGAATGATTTTATTTGAAGATTTAAATGAACTAAGAAAGCTTATAGAAAAAAATAGACTCTTAGAAA
CAGAGCTTGAACATTACAAAAAAACATTAAGAAAAATTCATGGTGCTAAGTATTCATGGGAAAATATCATTGGAAAAAGT
GATGCAATAGTTGAATGTAAGAAAAAAGCTATGAAAATGGCTAATATGGATTCCAATATTCTGATATATGGAGAAAGTGG
AGTAGGAAAAGAACTTTTTGCTCACGCTATCCATAATTCAAGTAAAAGAAGAGATTATCCCTTTGTAACTGTAAATTGCG
CTGCTATACCAGAACAATTAATGGAATCAGAGCTATTTGGGTATGAAGAAGGAGCATTTACAGGAGCGCAAAAAGGAGGT
AAAATAGGTAAGTTTGAACTTGCGAATCACGGTACTATTTTTTTAGATGAAATAGGTGATATGCCGTATACAATGCAGGC
AAAACTTTTAAGAGTATTGCAGGAAGGTGAAATCGAGAGAGTAGGAGGAAAAGCACCTATTAAAGTTGATGTACGGGTGA
TATCTGCAACAAACAAAGACCTTTCGAAACTTGTTAAAGAAGGTAAGTTTAGAAGTGATTTATTTTACAGAATAAATGTA
TTAATGCTTAATGTTCCACCATTGAGAGAAAGGAAAGAAGATATTCCGTTATTAATAGACCACTTTTTATCTCTTTTAAC
ACAAAATTCTGGAATATACAAAAAAGTTTCTAAAGAAGTATACGATATCTTAGAAAAATACGACTGGCCGGGTAATATAA
GAGAATTAAGAAATGTAATTGAAAGAATGGTTGTTAACTCAGAAGGAGATATAATTAGAAAAACAGATATTCCGCTTTAC
ATTTTAAAAAAAGAATTGCCAGTGAGAAAGAAAGGTTCGGGATTACAAGAAATGTTAGAGGAATTTGAGAAAGAGATAAT
TTTTGAGACGTTAAAAGAATGCAATTATAACAAGTCACAGGCAGCAGAAATATTAAAAATTCCAAGGTCAAGGCTTTATA
GGAAACTAAAAAAATTTGGGATTCTAGAAGAAAATAATGAAGTGAAATAA

Upstream 100 bases:

>100_bases
GGGGGTTATTGACTTTTTATAACGATTACGTGTATAATGGACATCAAAAGATGTACTAAAATGATACATCCTTCCTTTAT
TATTTAGGGGGCGTGAAAAA

Downstream 100 bases:

>100_bases
TACAGTGTAGCGAAATGATACATATTTTAAAAAGCACAGGAGATACCTCTCTTGTGCTTTTTTTTGTATTATTATAGGAC
AGTTATAAAAGCTCTAGTAG

Product: transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 469; Mature: 468

Protein sequence:

>469_residues
MSKNIDKSFLFSEEMEFLFESVFDKLPIAIDILDAEGNIRMINKTFLDYLGLEKDKVINKYVLDVDRNSRFPLVLKTGQN
EIAYRHKFANGKEAIVHRIAIKDGDEVIGGFGMILFEDLNELRKLIEKNRLLETELEHYKKTLRKIHGAKYSWENIIGKS
DAIVECKKKAMKMANMDSNILIYGESGVGKELFAHAIHNSSKRRDYPFVTVNCAAIPEQLMESELFGYEEGAFTGAQKGG
KIGKFELANHGTIFLDEIGDMPYTMQAKLLRVLQEGEIERVGGKAPIKVDVRVISATNKDLSKLVKEGKFRSDLFYRINV
LMLNVPPLRERKEDIPLLIDHFLSLLTQNSGIYKKVSKEVYDILEKYDWPGNIRELRNVIERMVVNSEGDIIRKTDIPLY
ILKKELPVRKKGSGLQEMLEEFEKEIIFETLKECNYNKSQAAEILKIPRSRLYRKLKKFGILEENNEVK

Sequences:

>Translated_469_residues
MSKNIDKSFLFSEEMEFLFESVFDKLPIAIDILDAEGNIRMINKTFLDYLGLEKDKVINKYVLDVDRNSRFPLVLKTGQN
EIAYRHKFANGKEAIVHRIAIKDGDEVIGGFGMILFEDLNELRKLIEKNRLLETELEHYKKTLRKIHGAKYSWENIIGKS
DAIVECKKKAMKMANMDSNILIYGESGVGKELFAHAIHNSSKRRDYPFVTVNCAAIPEQLMESELFGYEEGAFTGAQKGG
KIGKFELANHGTIFLDEIGDMPYTMQAKLLRVLQEGEIERVGGKAPIKVDVRVISATNKDLSKLVKEGKFRSDLFYRINV
LMLNVPPLRERKEDIPLLIDHFLSLLTQNSGIYKKVSKEVYDILEKYDWPGNIRELRNVIERMVVNSEGDIIRKTDIPLY
ILKKELPVRKKGSGLQEMLEEFEKEIIFETLKECNYNKSQAAEILKIPRSRLYRKLKKFGILEENNEVK
>Mature_468_residues
SKNIDKSFLFSEEMEFLFESVFDKLPIAIDILDAEGNIRMINKTFLDYLGLEKDKVINKYVLDVDRNSRFPLVLKTGQNE
IAYRHKFANGKEAIVHRIAIKDGDEVIGGFGMILFEDLNELRKLIEKNRLLETELEHYKKTLRKIHGAKYSWENIIGKSD
AIVECKKKAMKMANMDSNILIYGESGVGKELFAHAIHNSSKRRDYPFVTVNCAAIPEQLMESELFGYEEGAFTGAQKGGK
IGKFELANHGTIFLDEIGDMPYTMQAKLLRVLQEGEIERVGGKAPIKVDVRVISATNKDLSKLVKEGKFRSDLFYRINVL
MLNVPPLRERKEDIPLLIDHFLSLLTQNSGIYKKVSKEVYDILEKYDWPGNIRELRNVIERMVVNSEGDIIRKTDIPLYI
LKKELPVRKKGSGLQEMLEEFEKEIIFETLKECNYNKSQAAEILKIPRSRLYRKLKKFGILEENNEVK

Specific function: Member Of The Two-Component Regulatory System Atos/Atoc Involved In The Transcriptional Regulation Of The Ato Genes For Acetoacetate Metabolism. Also An Inhibitor Of Polyamine Biosynthesis. [C]

COG id: COG3829

COG function: function code KT; Transcriptional regulator containing PAS, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=405, Percent_Identity=43.9506172839506, Blast_Score=310, Evalue=1e-85,
Organism=Escherichia coli, GI1789233, Length=460, Percent_Identity=38.2608695652174, Blast_Score=301, Evalue=5e-83,
Organism=Escherichia coli, GI1790437, Length=328, Percent_Identity=45.1219512195122, Blast_Score=277, Evalue=9e-76,
Organism=Escherichia coli, GI1790299, Length=390, Percent_Identity=38.4615384615385, Blast_Score=265, Evalue=6e-72,
Organism=Escherichia coli, GI87082117, Length=324, Percent_Identity=45.0617283950617, Blast_Score=263, Evalue=1e-71,
Organism=Escherichia coli, GI1789087, Length=388, Percent_Identity=39.6907216494845, Blast_Score=262, Evalue=4e-71,
Organism=Escherichia coli, GI1786524, Length=384, Percent_Identity=38.0208333333333, Blast_Score=256, Evalue=2e-69,
Organism=Escherichia coli, GI87082152, Length=320, Percent_Identity=42.8125, Blast_Score=244, Evalue=1e-65,
Organism=Escherichia coli, GI1788905, Length=311, Percent_Identity=40.5144694533762, Blast_Score=233, Evalue=1e-62,
Organism=Escherichia coli, GI87081872, Length=321, Percent_Identity=39.8753894080997, Blast_Score=230, Evalue=2e-61,
Organism=Escherichia coli, GI1787583, Length=321, Percent_Identity=38.006230529595, Blast_Score=218, Evalue=5e-58,
Organism=Escherichia coli, GI87081858, Length=284, Percent_Identity=32.0422535211268, Blast_Score=164, Evalue=2e-41,
Organism=Escherichia coli, GI1789828, Length=215, Percent_Identity=35.8139534883721, Blast_Score=124, Evalue=1e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR001610
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR002078 [H]

Pfam domain/function: PF02954 HTH_8; PF00989 PAS; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 54056; Mature: 53924

Theoretical pI: Translated: 8.62; Mature: 8.62

Prosite motif: PS50112 PAS ; PS00675 SIGMA54_INTERACT_1 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKNIDKSFLFSEEMEFLFESVFDKLPIAIDILDAEGNIRMINKTFLDYLGLEKDKVINK
CCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCEEEHHHHHHHHHCCCHHHHHHH
YVLDVDRNSRFPLVLKTGQNEIAYRHKFANGKEAIVHRIAIKDGDEVIGGFGMILFEDLN
HHEECCCCCCCCEEEECCCCHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
ELRKLIEKNRLLETELEHYKKTLRKIHGAKYSWENIIGKSDAIVECKKKAMKMANMDSNI
HHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCHHHHHHHHHHHHHCCCCCE
LIYGESGVGKELFAHAIHNSSKRRDYPFVTVNCAAIPEQLMESELFGYEEGAFTGAQKGG
EEEECCCCCHHHHHHHHHCCCCCCCCCEEEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCC
KIGKFELANHGTIFLDEIGDMPYTMQAKLLRVLQEGEIERVGGKAPIKVDVRVISATNKD
CCCEEEECCCCEEEEECCCCCCHHHHHHHHHHHHCCCHHHCCCCCCEEEEEEEEECCCHH
LSKLVKEGKFRSDLFYRINVLMLNVPPLRERKEDIPLLIDHFLSLLTQNSGIYKKVSKEV
HHHHHHCCCCHHHHEEEEEEEEECCCCCHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHH
YDILEKYDWPGNIRELRNVIERMVVNSEGDIIRKTDIPLYILKKELPVRKKGSGLQEMLE
HHHHHHCCCCCCHHHHHHHHHHHHCCCCCCEEEECCCCEEEEECCCCCCCCCCCHHHHHH
EFEKEIIFETLKECNYNKSQAAEILKIPRSRLYRKLKKFGILEENNEVK
HHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
SKNIDKSFLFSEEMEFLFESVFDKLPIAIDILDAEGNIRMINKTFLDYLGLEKDKVINK
CCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCEEEHHHHHHHHHCCCHHHHHHH
YVLDVDRNSRFPLVLKTGQNEIAYRHKFANGKEAIVHRIAIKDGDEVIGGFGMILFEDLN
HHEECCCCCCCCEEEECCCCHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
ELRKLIEKNRLLETELEHYKKTLRKIHGAKYSWENIIGKSDAIVECKKKAMKMANMDSNI
HHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCHHHHHHHHHHHHHCCCCCE
LIYGESGVGKELFAHAIHNSSKRRDYPFVTVNCAAIPEQLMESELFGYEEGAFTGAQKGG
EEEECCCCCHHHHHHHHHCCCCCCCCCEEEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCC
KIGKFELANHGTIFLDEIGDMPYTMQAKLLRVLQEGEIERVGGKAPIKVDVRVISATNKD
CCCEEEECCCCEEEEECCCCCCHHHHHHHHHHHHCCCHHHCCCCCCEEEEEEEEECCCHH
LSKLVKEGKFRSDLFYRINVLMLNVPPLRERKEDIPLLIDHFLSLLTQNSGIYKKVSKEV
HHHHHHCCCCHHHHEEEEEEEEECCCCCHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHH
YDILEKYDWPGNIRELRNVIERMVVNSEGDIIRKTDIPLYILKKELPVRKKGSGLQEMLE
HHHHHHCCCCCCHHHHHHHHHHHHCCCCCCEEEECCCCEEEEECCCCCCCCCCCHHHHHH
EFEKEIIFETLKECNYNKSQAAEILKIPRSRLYRKLKKFGILEENNEVK
HHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA