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The map label for this gene is PrfB [H]

Identifier: 20807024

GI number: 20807024

Start: 531083

End: 532126

Strand: Direct

Name: PrfB [H]

Synonym: TTE0523

Alternate gene names: 20807024

Gene position: 531083-532126 (Clockwise)

Preceding gene: 20807023

Following gene: 20807025

Centisome position: 19.75

GC content: 41.57

Gene sequence:

>1044_bases
GTGTTTCTCTTTGACGTGGAAGGGATAAAAAAAGAGATAGAGGATATAGAAAAGCAGATGTCAGATCCTTCTTTCTGGAG
CGATTTGAAAAAATCTCAGGAACTTTCTAAAAAGCAAAAAGCGTTGAAAGAACTTCTGGAAGAATATGAATCCCTTCTTT
CTCGCTGTGAAGACTTGTACACTCTAATTGAGCTAGGTTTGGAAGAAGGGGATGAATCTATCGCTGAAGAGGTGGAGAGG
GAATACAAAGAGCTTAAAAAAGAGGTTGAAAATCTTAAAATACGTACTCTTTTGAGTGGGCCATATGACAAAAACAATGC
TATACTCTCTATTCACGCAGGGTCAGGTGGCACTGAAGCTCAGGACTGGGCTGAAATGCTTCTTAGGATGTACACCAGAT
GGGCTAATGATAAGGGATTTGAAGTAGAAATACTGGATTATCTGGAAGGGGAAGAAGCCGGCATAAAGAGTGCTACTCTT
CTCATAAAGGGACCCTTTGCCTATGGTTATCTGAAGGGAGAGGCAGGAGTTCACAGACTTGTCAGGATTTCTCCTTTTGA
TGCAGCAGGCAGGCGCCATACCTCTTTTGCTCTGGTAGAAGTTATACCTGAGCTTGAAGATGATATAGAAATTGAAATAA
GGCCTGAAGATTTAAAAATTGATACGTTCAGGGCATCAGGCGCAGGGGGGCAGCATGTTAACAAGACAGAGTCTGCAGTG
AGGATTACTCACCTTCCGACAGGGATAGTTGTCTCATGTCAGAATGAGAGGTCTCAGATACAGAATAGAGAGACTGCTAT
GAAAATGCTCAAAGCAAAGCTTTTGGATTTGATGATGAAAGAGAGAAAAGAAAAGATAGAGGACCTAAAGGGAGAGCACA
GAGAGGCTGCATGGGGTAACCAGATAAGGTCTTATGTTTTTCAGCCCTATACTCTTGTAAAAGACCACAGGACAAATTAT
GAAGAAGGAAATGTAAATGCCGTTATGGACGGAGAGATAGATGGGTTTATAAATGCTTATTTAAAGCAAAAGAGCGCATC
CTGA

Upstream 100 bases:

>100_bases
GCGGAGCTAATGTAAAATAAAGGGAAAAGGAGAGGATGAGAATTGCTGCAGGATTACAGGGCAGAAGTTGAGCAAATGCT
TCATGAGATAAAAGAAATGG

Downstream 100 bases:

>100_bases
TGGATGCCTCTTTTTTATTCTTACTAAAAAATTTTTTCTTGCCACCCCTAAAAATTTCGATTCCCTTCCGAAATAATATA
GTGAAAACAAAAACAAAGGA

Product: protein chain release factor B

Products: NA

Alternate protein names: RF-2 [H]

Number of amino acids: Translated: 347; Mature: 347

Protein sequence:

>347_residues
MFLFDVEGIKKEIEDIEKQMSDPSFWSDLKKSQELSKKQKALKELLEEYESLLSRCEDLYTLIELGLEEGDESIAEEVER
EYKELKKEVENLKIRTLLSGPYDKNNAILSIHAGSGGTEAQDWAEMLLRMYTRWANDKGFEVEILDYLEGEEAGIKSATL
LIKGPFAYGYLKGEAGVHRLVRISPFDAAGRRHTSFALVEVIPELEDDIEIEIRPEDLKIDTFRASGAGGQHVNKTESAV
RITHLPTGIVVSCQNERSQIQNRETAMKMLKAKLLDLMMKERKEKIEDLKGEHREAAWGNQIRSYVFQPYTLVKDHRTNY
EEGNVNAVMDGEIDGFINAYLKQKSAS

Sequences:

>Translated_347_residues
MFLFDVEGIKKEIEDIEKQMSDPSFWSDLKKSQELSKKQKALKELLEEYESLLSRCEDLYTLIELGLEEGDESIAEEVER
EYKELKKEVENLKIRTLLSGPYDKNNAILSIHAGSGGTEAQDWAEMLLRMYTRWANDKGFEVEILDYLEGEEAGIKSATL
LIKGPFAYGYLKGEAGVHRLVRISPFDAAGRRHTSFALVEVIPELEDDIEIEIRPEDLKIDTFRASGAGGQHVNKTESAV
RITHLPTGIVVSCQNERSQIQNRETAMKMLKAKLLDLMMKERKEKIEDLKGEHREAAWGNQIRSYVFQPYTLVKDHRTNY
EEGNVNAVMDGEIDGFINAYLKQKSAS
>Mature_347_residues
MFLFDVEGIKKEIEDIEKQMSDPSFWSDLKKSQELSKKQKALKELLEEYESLLSRCEDLYTLIELGLEEGDESIAEEVER
EYKELKKEVENLKIRTLLSGPYDKNNAILSIHAGSGGTEAQDWAEMLLRMYTRWANDKGFEVEILDYLEGEEAGIKSATL
LIKGPFAYGYLKGEAGVHRLVRISPFDAAGRRHTSFALVEVIPELEDDIEIEIRPEDLKIDTFRASGAGGQHVNKTESAV
RITHLPTGIVVSCQNERSQIQNRETAMKMLKAKLLDLMMKERKEKIEDLKGEHREAAWGNQIRSYVFQPYTLVKDHRTNY
EEGNVNAVMDGEIDGFINAYLKQKSAS

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=297, Percent_Identity=39.0572390572391, Blast_Score=182, Evalue=3e-46,
Organism=Homo sapiens, GI34577120, Length=305, Percent_Identity=36.3934426229508, Blast_Score=176, Evalue=3e-44,
Organism=Homo sapiens, GI166795305, Length=211, Percent_Identity=40.2843601895735, Blast_Score=134, Evalue=1e-31,
Organism=Escherichia coli, GI2367172, Length=339, Percent_Identity=48.377581120944, Blast_Score=338, Evalue=3e-94,
Organism=Escherichia coli, GI1787462, Length=355, Percent_Identity=35.7746478873239, Blast_Score=195, Evalue=4e-51,
Organism=Caenorhabditis elegans, GI17542784, Length=247, Percent_Identity=36.0323886639676, Blast_Score=117, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6321295, Length=360, Percent_Identity=33.8888888888889, Blast_Score=164, Evalue=2e-41,
Organism=Drosophila melanogaster, GI19921226, Length=237, Percent_Identity=40.9282700421941, Blast_Score=166, Evalue=2e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 39587; Mature: 39587

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFLFDVEGIKKEIEDIEKQMSDPSFWSDLKKSQELSKKQKALKELLEEYESLLSRCEDLY
CEEEEHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TLIELGLEEGDESIAEEVEREYKELKKEVENLKIRTLLSGPYDKNNAILSIHAGSGGTEA
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCEEEEEECCCCCCCH
QDWAEMLLRMYTRWANDKGFEVEILDYLEGEEAGIKSATLLIKGPFAYGYLKGEAGVHRL
HHHHHHHHHHHHHHCCCCCCEEEEEHHCCCCCCCCCEEEEEEECCCEEEEEECCCCHHHE
VRISPFDAAGRRHTSFALVEVIPELEDDIEIEIRPEDLKIDTFRASGAGGQHVNKTESAV
EEECCCCCCCCCCHHHHHHHHHHCCCCCCEEEEECCCEEEEEEECCCCCCCCCCCCCCEE
RITHLPTGIVVSCQNERSQIQNRETAMKMLKAKLLDLMMKERKEKIEDLKGEHREAAWGN
EEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
QIRSYVFQPYTLVKDHRTNYEEGNVNAVMDGEIDGFINAYLKQKSAS
HHHHHHCCCHHHHHHCCCCCCCCCEEEEECCCHHHHHHHHHHHCCCC
>Mature Secondary Structure
MFLFDVEGIKKEIEDIEKQMSDPSFWSDLKKSQELSKKQKALKELLEEYESLLSRCEDLY
CEEEEHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TLIELGLEEGDESIAEEVEREYKELKKEVENLKIRTLLSGPYDKNNAILSIHAGSGGTEA
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCEEEEEECCCCCCCH
QDWAEMLLRMYTRWANDKGFEVEILDYLEGEEAGIKSATLLIKGPFAYGYLKGEAGVHRL
HHHHHHHHHHHHHHCCCCCCEEEEEHHCCCCCCCCCEEEEEEECCCEEEEEECCCCHHHE
VRISPFDAAGRRHTSFALVEVIPELEDDIEIEIRPEDLKIDTFRASGAGGQHVNKTESAV
EEECCCCCCCCCCHHHHHHHHHHCCCCCCEEEEECCCEEEEEEECCCCCCCCCCCCCCEE
RITHLPTGIVVSCQNERSQIQNRETAMKMLKAKLLDLMMKERKEKIEDLKGEHREAAWGN
EEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
QIRSYVFQPYTLVKDHRTNYEEGNVNAVMDGEIDGFINAYLKQKSAS
HHHHHHCCCHHHHHHCCCCCCCCCEEEEECCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]