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The map label for this gene is ArtI [H]

Identifier: 20807013

GI number: 20807013

Start: 519808

End: 520590

Strand: Direct

Name: ArtI [H]

Synonym: TTE0512

Alternate gene names: 20807013

Gene position: 519808-520590 (Clockwise)

Preceding gene: 20807012

Following gene: 20807014

Centisome position: 19.33

GC content: 38.19

Gene sequence:

>783_bases
ATGAATAAAAAATCACTATTTTTAGCTTTTGCTGTAGTTTTTGCTCTGGCTTTTATGCTTTCAGGCTGTGGCAGTAAGTT
TAATACGGTAGATCAAATCAAGCAAAAAGGCGTAATTGTGATGGGCACGAGTGCAGATTTTCCGCCTTTTGAATTCCACA
AAGTAGAAGGAGGAAAAGACGAGATAGTAGGTTTTGATATTGATATTGCTAATGCTATAGCGAAAAAGCTGGGAGTGAAA
CTTGAAATAAAAGATATGGACTTTAAAGGCCTTATTCCAGCTCTTCAGGCTGGAAGAGTTGATATGGTAATTGCGGGTAT
GACGCCTACAGCAGAGAGAAAAAAGAGTGTAGATTTTTCAGACCTCTATTACGACAGCAGGCAAGTTGTTGTGGTAAAGA
ATGACAGTCCAATTTCAAAATTTGACGATTTGAAAGTCAAAACAATTGCCGTTCAAATAGGAACTACTTCGGAAGAAGCT
GCTAAAAAGATTCCTAATGTAAAGTTAAAGCAGTTAAATCGAGTCAGTGATGAATTTATGGACCTTCAAAATGGCCGCTG
TGATGCGATTGTCGTAGAAGACACAGTTGCTAAAGCTTATTTGAAGGAATACAAGGATATGAAAATTCTCTATATGGATG
AGATAAACAATGTGGAGAATGGTTCTGCTGTAGCAGTGGCAAAGGGCAATAAGAGCCTTTTGGATGTGGTGAATGAGGTG
ATAAAAGAGCTCAAGCAAAGTGGAGAGTACGACAAACTTGTAGATAAGTGGTTTAAGCAGTAG

Upstream 100 bases:

>100_bases
ATTCATAAGATATTATAATTATAAAAAGTGAAATCGTCAAAAGAAATTAAGCAGTATGGAGAAATTTTCAGAAAAATTAA
AAAAGGGAGAGGAGAAAGAT

Downstream 100 bases:

>100_bases
TTTAAAGTGGAGAGTTTTTCTCCACTAAAATTTTGTGAAGGAGAGAAGGTAGGCTATGACAGTAGACTTTTTGAGCATGG
TAAAATACACGCCACTTTTT

Product: ABC-type amino acid transport system, periplasmic component

Products: ADP; phosphate; L-glutamine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MNKKSLFLAFAVVFALAFMLSGCGSKFNTVDQIKQKGVIVMGTSADFPPFEFHKVEGGKDEIVGFDIDIANAIAKKLGVK
LEIKDMDFKGLIPALQAGRVDMVIAGMTPTAERKKSVDFSDLYYDSRQVVVVKNDSPISKFDDLKVKTIAVQIGTTSEEA
AKKIPNVKLKQLNRVSDEFMDLQNGRCDAIVVEDTVAKAYLKEYKDMKILYMDEINNVENGSAVAVAKGNKSLLDVVNEV
IKELKQSGEYDKLVDKWFKQ

Sequences:

>Translated_260_residues
MNKKSLFLAFAVVFALAFMLSGCGSKFNTVDQIKQKGVIVMGTSADFPPFEFHKVEGGKDEIVGFDIDIANAIAKKLGVK
LEIKDMDFKGLIPALQAGRVDMVIAGMTPTAERKKSVDFSDLYYDSRQVVVVKNDSPISKFDDLKVKTIAVQIGTTSEEA
AKKIPNVKLKQLNRVSDEFMDLQNGRCDAIVVEDTVAKAYLKEYKDMKILYMDEINNVENGSAVAVAKGNKSLLDVVNEV
IKELKQSGEYDKLVDKWFKQ
>Mature_260_residues
MNKKSLFLAFAVVFALAFMLSGCGSKFNTVDQIKQKGVIVMGTSADFPPFEFHKVEGGKDEIVGFDIDIANAIAKKLGVK
LEIKDMDFKGLIPALQAGRVDMVIAGMTPTAERKKSVDFSDLYYDSRQVVVVKNDSPISKFDDLKVKTIAVQIGTTSEEA
AKKIPNVKLKQLNRVSDEFMDLQNGRCDAIVVEDTVAKAYLKEYKDMKILYMDEINNVENGSAVAVAKGNKSLLDVVNEV
IKELKQSGEYDKLVDKWFKQ

Specific function: Part of a binding-protein-dependent transport system for arginine [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1787031, Length=223, Percent_Identity=38.1165919282511, Blast_Score=137, Evalue=5e-34,
Organism=Escherichia coli, GI1787085, Length=226, Percent_Identity=35.3982300884956, Blast_Score=137, Evalue=8e-34,
Organism=Escherichia coli, GI1788228, Length=232, Percent_Identity=33.1896551724138, Blast_Score=128, Evalue=3e-31,
Organism=Escherichia coli, GI1787088, Length=227, Percent_Identity=31.7180616740088, Blast_Score=117, Evalue=8e-28,
Organism=Escherichia coli, GI1788649, Length=257, Percent_Identity=27.6264591439689, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1788648, Length=234, Percent_Identity=29.0598290598291, Blast_Score=89, Evalue=2e-19,
Organism=Escherichia coli, GI1786876, Length=247, Percent_Identity=27.5303643724696, Blast_Score=85, Evalue=5e-18,

Paralogues:

None

Copy number: 1440 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 460 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015683
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 28850; Mature: 28850

Theoretical pI: Translated: 7.25; Mature: 7.25

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00211 ABC_TRANSPORTER_1 ; PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKKSLFLAFAVVFALAFMLSGCGSKFNTVDQIKQKGVIVMGTSADFPPFEFHKVEGGKD
CCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCEEEEECCCCCCCCEEEECCCCCC
EIVGFDIDIANAIAKKLGVKLEIKDMDFKGLIPALQAGRVDMVIAGMTPTAERKKSVDFS
CEEEEEHHHHHHHHHHHCCEEEEECCCCHHCHHHHHCCCEEEEEECCCCCHHHHCCCCHH
DLYYDSRQVVVVKNDSPISKFDDLKVKTIAVQIGTTSEEAAKKIPNVKLKQLNRVSDEFM
HHEECCEEEEEEECCCCCCHHCCCEEEEEEEEECCCCHHHHHHCCCCHHHHHHHHHHHHH
DLQNGRCDAIVVEDTVAKAYLKEYKDMKILYMDEINNVENGSAVAVAKGNKSLLDVVNEV
HHCCCCCCEEEEHHHHHHHHHHHHCCCEEEEEECCCCCCCCCEEEEECCCHHHHHHHHHH
IKELKQSGEYDKLVDKWFKQ
HHHHHHCCCHHHHHHHHHCC
>Mature Secondary Structure
MNKKSLFLAFAVVFALAFMLSGCGSKFNTVDQIKQKGVIVMGTSADFPPFEFHKVEGGKD
CCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCEEEEECCCCCCCCEEEECCCCCC
EIVGFDIDIANAIAKKLGVKLEIKDMDFKGLIPALQAGRVDMVIAGMTPTAERKKSVDFS
CEEEEEHHHHHHHHHHHCCEEEEECCCCHHCHHHHHCCCEEEEEECCCCCHHHHCCCCHH
DLYYDSRQVVVVKNDSPISKFDDLKVKTIAVQIGTTSEEAAKKIPNVKLKQLNRVSDEFM
HHEECCEEEEEEECCCCCCHHCCCEEEEEEEEECCCCHHHHHHCCCCHHHHHHHHHHHHH
DLQNGRCDAIVVEDTVAKAYLKEYKDMKILYMDEINNVENGSAVAVAKGNKSLLDVVNEV
HHCCCCCCEEEEHHHHHHHHHHHHCCCEEEEEECCCCCCCCCEEEEECCCHHHHHHHHHH
IKELKQSGEYDKLVDKWFKQ
HHHHHHCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-glutamine [Periplasm]; H2O [C]

Specific reaction: ATP + L-glutamine [Periplasm] + H2O = ADP + phosphate + L-glutamine [Cytoplasm] [C]

General reaction: Translocates substrate across the membrane [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377 [H]