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The map label for this gene is MrcA [H]

Identifier: 20806831

GI number: 20806831

Start: 329259

End: 331790

Strand: Direct

Name: MrcA [H]

Synonym: TTE0314

Alternate gene names: 20806831

Gene position: 329259-331790 (Clockwise)

Preceding gene: 20806830

Following gene: 20806832

Centisome position: 12.24

GC content: 41.71

Gene sequence:

>2532_bases
ATGGACAAAAATAGCGATTTAAACCTAAAAAGAAGCCGGCGAAAAAAGAATAGTGAAAAAAAGAAAAAAAGCTCCATTAA
GCTTTTTTTCAAATGGGTTGGAATTGCTTTTCTCATTCTGTTTTTTGCAGCGGTTGGGGCAATCGGCGGAAAAGTCTTGG
CTATAATTAAAAATACTCCTCCAATTTCTCAGGAAGCTTTGACGTCTCAGGCGCAGTCTTCTGTTGTTTATGCAAAAGAC
GAAAATGGTAACTGGCAGAGAGTAGCAATACTTCATGGTGCTGATAACAGGCTGTGGGTTCCAATAGAGAAAATACCCAA
GAATTTGCAAAATGCTTTTGTGGCAATAGAGGACCAGCGTTTTTATAAGAACAAACTAGGAATTGACCCAAAGAGAATTA
TTGGAGCTCTTATTGCAGATATTATGGCGGGAGGAAAGCCTGTAGAGGGAGCCAGTACTATTACACAGCAACTTGTGAAA
AACACCATGCTGTCCAGTGAAAAAACTATTACTAGAAAAATACAGGAAATGGTTTTGGCTTGGAGATTGGAGCAGATATA
TTCAAAGCAACAGATACTGGAAGCTTATCTTAATACCATCTATTTAGGCGGTCCCAATGTGAATGCCTATGGTGTCCAGG
CAGCTTCTTTAGCTTATTTTGGCAAAGATGTGAGCCAGTTAGACCTTGCGGAATGTGCTATGATTGCGGGAATTACAAAG
AATCCATCCCGTTATTCTCCTTATGCTGATCTAGAAGCCGCTACAGAAAGGCAGCATTTAGTGTTAAAGAAAATGCTGGA
ACAAGGCTATATAACAAAAGAAGAGTATGAAAAGGCAATCTCAGAAAAATTGGTTTTCAAGAAAACCAATTTTACCACTT
ATGAGCATAAATACTTTATTGACCAAGTTATAAATGATGTGGCAGATGCTTTGTCTCAGCACCTTAACATTTCCCACGAT
GAGGCAATAAAGAAAATATACAACGGGGGTTTGAGAATTTATTCTACTATGGATTTGCAAATACAAAAAACAATGGAAGA
AGCTTTTAAAAATCCAAAATTGTTCCCTCCTGATATAAAGGATAGAAGTGGGAATACGAGAATAGTTCAAGGTGCAATGG
TAGTAATGGACTGGAAGACTGGCGAGGTAAAGGGAATTGTAGGAGGCAGGGAAACAGAAGAGACTAAAGGTAAGACAAGG
ATATTTAACTATGCAACACAAGGTGGAAGACCAGCTGGTTCTTCTATAAAACCTCTTACAGTTTACGGACCTGCTTTAGA
GAAAGGTTATACGGCAGCTACTGTTGTGGATGATGTCCCTGTTTTTTATAAACAATGGAACTGGGAGCCCCATAACTACG
AAAGCGGGAAGTACAGAGGGCTTATGACCTTTAGAGAGGCTTTAACTTATTCCGAAAATGTTCCTGCGGCGAGGATAGTT
GTAGAAATGATTGGACTTCATACTGCAGCGGAATATGGAAAGAAATTTGGACTTACTGTAACAAAAGATGTTGCCTCTTC
TCCTGCTGCTCTTGCATTAGGAGCGGGAGGCGCAGTTACACCCTTACAGGAAGCTGCAGCTTACGGTGCCATTGCCAATG
GAGGAGTATATACTACTCCTATCACTTTTACAAAAGTCACTGATTCAAAAGGAAAAGTAATTTTGGAAAACAAGCCTTCA
CAGCATATGGTCTTAACGCCACAGAACGCATACATTTTAACAAGTATGATGCAGGACGTAGTGAAATACGGGACAGGTAC
AAATGCAAGGTTGCCTAATATGCCGGTGGCAGGTAAAACAGGTACAAATGAAAGTTACAACGATGCATGGTTTACAGGCT
TTACTCCTTACTACGTTGGGTCTGTGTGGATGGGTACAGGGGAAAACATTCCTATGATATATGGAGGGAGGGGAGTTGTA
GGAGGCTCCTATCCAGCCATGCTATGGAGAACAGTCATGGCAGAGATACACAAAAGCTTGCCTTACAAAGATTTTGAAAG
ACCTCCAGGTATTATTTCTGTGACAGTGTGCAAAGATTCTGGGGAGCTTCCAACTGACCTGTGCCATCAAGACCTGAGAG
GCGACAGAACCTATACTGAAATTTTTGCAGAAGGCACGCAACCCACTACCTACTGTACTGTCCATGTGCTGGCAAAAATT
AATTCACAAAATGGCAAATTGGCGACAGAATTTACTCCACCAGATTTAGTTAAAGAGGCTGTGTTCATTGACCCTCCAGG
CAGGACTCCTGAGCAAAATGCTGTGGCGCTAGACGGCAAATATGTGGTGCCAAAGGATTATGACGACACACCTCCACCAG
CACAACCACCGTCGGGAGAAGTGTTGCCGCCAGAGAATACGCAAAATCCACCATCTCAGCCTCCGAATGAGATACAACAG
CCTCCCGGGAATACTCCTCCCAATAATGGAGGGAATAACGGTACTACACAGAATCCGCCTCAGAATAACAACAATAATAC
GTCAAGTAACCCTCTGGAAAAGATCTTTCCTCCTAAAAATAGCAAGCCTTGA

Upstream 100 bases:

>100_bases
CTGACTTTTCTTTTTTGTTACAATTTGGACATAAAAAAGTGATATAATTTATGATATAATCAATATGATTGGATAAACCA
TAATTTTATGGGGGATCAGT

Downstream 100 bases:

>100_bases
GCAATATCAAAAGAGTCCACTCCAAGTAGAGGGTGGGCTCTTTTGAAATTTTGTGGTATATTTAATGTAAAGAGCTTGTG
AGAGGGGGCTTTTAGTTTGA

Product: membrane carboxypeptidase (penicillin-binding protein)

Products: NA

Alternate protein names: PBP1a; Penicillin-insensitive transglycosylase; Peptidoglycan TGase; Penicillin-sensitive transpeptidase; DD-transpeptidase [H]

Number of amino acids: Translated: 843; Mature: 843

Protein sequence:

>843_residues
MDKNSDLNLKRSRRKKNSEKKKKSSIKLFFKWVGIAFLILFFAAVGAIGGKVLAIIKNTPPISQEALTSQAQSSVVYAKD
ENGNWQRVAILHGADNRLWVPIEKIPKNLQNAFVAIEDQRFYKNKLGIDPKRIIGALIADIMAGGKPVEGASTITQQLVK
NTMLSSEKTITRKIQEMVLAWRLEQIYSKQQILEAYLNTIYLGGPNVNAYGVQAASLAYFGKDVSQLDLAECAMIAGITK
NPSRYSPYADLEAATERQHLVLKKMLEQGYITKEEYEKAISEKLVFKKTNFTTYEHKYFIDQVINDVADALSQHLNISHD
EAIKKIYNGGLRIYSTMDLQIQKTMEEAFKNPKLFPPDIKDRSGNTRIVQGAMVVMDWKTGEVKGIVGGRETEETKGKTR
IFNYATQGGRPAGSSIKPLTVYGPALEKGYTAATVVDDVPVFYKQWNWEPHNYESGKYRGLMTFREALTYSENVPAARIV
VEMIGLHTAAEYGKKFGLTVTKDVASSPAALALGAGGAVTPLQEAAAYGAIANGGVYTTPITFTKVTDSKGKVILENKPS
QHMVLTPQNAYILTSMMQDVVKYGTGTNARLPNMPVAGKTGTNESYNDAWFTGFTPYYVGSVWMGTGENIPMIYGGRGVV
GGSYPAMLWRTVMAEIHKSLPYKDFERPPGIISVTVCKDSGELPTDLCHQDLRGDRTYTEIFAEGTQPTTYCTVHVLAKI
NSQNGKLATEFTPPDLVKEAVFIDPPGRTPEQNAVALDGKYVVPKDYDDTPPPAQPPSGEVLPPENTQNPPSQPPNEIQQ
PPGNTPPNNGGNNGTTQNPPQNNNNNTSSNPLEKIFPPKNSKP

Sequences:

>Translated_843_residues
MDKNSDLNLKRSRRKKNSEKKKKSSIKLFFKWVGIAFLILFFAAVGAIGGKVLAIIKNTPPISQEALTSQAQSSVVYAKD
ENGNWQRVAILHGADNRLWVPIEKIPKNLQNAFVAIEDQRFYKNKLGIDPKRIIGALIADIMAGGKPVEGASTITQQLVK
NTMLSSEKTITRKIQEMVLAWRLEQIYSKQQILEAYLNTIYLGGPNVNAYGVQAASLAYFGKDVSQLDLAECAMIAGITK
NPSRYSPYADLEAATERQHLVLKKMLEQGYITKEEYEKAISEKLVFKKTNFTTYEHKYFIDQVINDVADALSQHLNISHD
EAIKKIYNGGLRIYSTMDLQIQKTMEEAFKNPKLFPPDIKDRSGNTRIVQGAMVVMDWKTGEVKGIVGGRETEETKGKTR
IFNYATQGGRPAGSSIKPLTVYGPALEKGYTAATVVDDVPVFYKQWNWEPHNYESGKYRGLMTFREALTYSENVPAARIV
VEMIGLHTAAEYGKKFGLTVTKDVASSPAALALGAGGAVTPLQEAAAYGAIANGGVYTTPITFTKVTDSKGKVILENKPS
QHMVLTPQNAYILTSMMQDVVKYGTGTNARLPNMPVAGKTGTNESYNDAWFTGFTPYYVGSVWMGTGENIPMIYGGRGVV
GGSYPAMLWRTVMAEIHKSLPYKDFERPPGIISVTVCKDSGELPTDLCHQDLRGDRTYTEIFAEGTQPTTYCTVHVLAKI
NSQNGKLATEFTPPDLVKEAVFIDPPGRTPEQNAVALDGKYVVPKDYDDTPPPAQPPSGEVLPPENTQNPPSQPPNEIQQ
PPGNTPPNNGGNNGTTQNPPQNNNNNTSSNPLEKIFPPKNSKP
>Mature_843_residues
MDKNSDLNLKRSRRKKNSEKKKKSSIKLFFKWVGIAFLILFFAAVGAIGGKVLAIIKNTPPISQEALTSQAQSSVVYAKD
ENGNWQRVAILHGADNRLWVPIEKIPKNLQNAFVAIEDQRFYKNKLGIDPKRIIGALIADIMAGGKPVEGASTITQQLVK
NTMLSSEKTITRKIQEMVLAWRLEQIYSKQQILEAYLNTIYLGGPNVNAYGVQAASLAYFGKDVSQLDLAECAMIAGITK
NPSRYSPYADLEAATERQHLVLKKMLEQGYITKEEYEKAISEKLVFKKTNFTTYEHKYFIDQVINDVADALSQHLNISHD
EAIKKIYNGGLRIYSTMDLQIQKTMEEAFKNPKLFPPDIKDRSGNTRIVQGAMVVMDWKTGEVKGIVGGRETEETKGKTR
IFNYATQGGRPAGSSIKPLTVYGPALEKGYTAATVVDDVPVFYKQWNWEPHNYESGKYRGLMTFREALTYSENVPAARIV
VEMIGLHTAAEYGKKFGLTVTKDVASSPAALALGAGGAVTPLQEAAAYGAIANGGVYTTPITFTKVTDSKGKVILENKPS
QHMVLTPQNAYILTSMMQDVVKYGTGTNARLPNMPVAGKTGTNESYNDAWFTGFTPYYVGSVWMGTGENIPMIYGGRGVV
GGSYPAMLWRTVMAEIHKSLPYKDFERPPGIISVTVCKDSGELPTDLCHQDLRGDRTYTEIFAEGTQPTTYCTVHVLAKI
NSQNGKLATEFTPPDLVKEAVFIDPPGRTPEQNAVALDGKYVVPKDYDDTPPPAQPPSGEVLPPENTQNPPSQPPNEIQQ
PPGNTPPNNGGNNGTTQNPPQNNNNNTSSNPLEKIFPPKNSKP

Specific function: Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal

COG id: COG0744

COG function: function code M; Membrane carboxypeptidase (penicillin-binding protein)

Gene ontology:

Cell location: Cell membrane; Single-pass type II membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786343, Length=557, Percent_Identity=34.1113105924596, Blast_Score=222, Evalue=8e-59,
Organism=Escherichia coli, GI87082258, Length=291, Percent_Identity=37.8006872852234, Blast_Score=177, Evalue=3e-45,
Organism=Escherichia coli, GI1788867, Length=776, Percent_Identity=26.6752577319588, Blast_Score=159, Evalue=8e-40,
Organism=Escherichia coli, GI1789601, Length=234, Percent_Identity=29.4871794871795, Blast_Score=87, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011816
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: 2.4.2.-; 3.4.-.-

Molecular weight: Translated: 92758; Mature: 92758

Theoretical pI: Translated: 9.21; Mature: 9.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKNSDLNLKRSRRKKNSEKKKKSSIKLFFKWVGIAFLILFFAAVGAIGGKVLAIIKNTP
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCC
PISQEALTSQAQSSVVYAKDENGNWQRVAILHGADNRLWVPIEKIPKNLQNAFVAIEDQR
CCCHHHHHHHHHCCEEEEECCCCCEEEEEEEECCCCEEEEEHHHHHHHHHHHEEEEECHH
FYKNKLGIDPKRIIGALIADIMAGGKPVEGASTITQQLVKNTMLSSEKTITRKIQEMVLA
HHHHHCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
WRLEQIYSKQQILEAYLNTIYLGGPNVNAYGVQAASLAYFGKDVSQLDLAECAMIAGITK
HHHHHHHHHHHHHHHHHHEEEECCCCCCEECCHHHHHHHHCCCHHHHHHHHHHHHHCCCC
NPSRYSPYADLEAATERQHLVLKKMLEQGYITKEEYEKAISEKLVFKKTNFTTYEHKYFI
CCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHEEEECCCCEEHHHHHH
DQVINDVADALSQHLNISHDEAIKKIYNGGLRIYSTMDLQIQKTMEEAFKNPKLFPPDIK
HHHHHHHHHHHHHHCCCCHHHHHHHHHCCCEEEEEEECHHHHHHHHHHHCCCCCCCCCCC
DRSGNTRIVQGAMVVMDWKTGEVKGIVGGRETEETKGKTRIFNYATQGGRPAGSSIKPLT
CCCCCEEEEEEEEEEEECCCCCEEEECCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCEE
VYGPALEKGYTAATVVDDVPVFYKQWNWEPHNYESGKYRGLMTFREALTYSENVPAARIV
EECCHHHCCCEEEEHHHCCCHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHH
VEMIGLHTAAEYGKKFGLTVTKDVASSPAALALGAGGAVTPLQEAAAYGAIANGGVYTTP
HHHHHHHHHHHHHHHCCCEEEHHHCCCCCEEEECCCCCCCHHHHHHHHCCCCCCCEEECC
ITFTKVTDSKGKVILENKPSQHMVLTPQNAYILTSMMQDVVKYGTGTNARLPNMPVAGKT
EEEEEEECCCCEEEEECCCCCCEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
GTNESYNDAWFTGFTPYYVGSVWMGTGENIPMIYGGRGVVGGSYPAMLWRTVMAEIHKSL
CCCCCCCCCEECCCCHHHHCEEEECCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHC
PYKDFERPPGIISVTVCKDSGELPTDLCHQDLRGDRTYTEIFAEGTQPTTYCTVHVLAKI
CCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHCCCCHHHHHHHCCCCCCEEEEEEEEEEE
NSQNGKLATEFTPPDLVKEAVFIDPPGRTPEQNAVALDGKYVVPKDYDDTPPPAQPPSGE
CCCCCEEEEECCCHHHHHHCEEECCCCCCCCCCEEEECCEEECCCCCCCCCCCCCCCCCC
VLPPENTQNPPSQPPNEIQQPPGNTPPNNGGNNGTTQNPPQNNNNNTSSNPLEKIFPPKN
CCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCC
SKP
CCC
>Mature Secondary Structure
MDKNSDLNLKRSRRKKNSEKKKKSSIKLFFKWVGIAFLILFFAAVGAIGGKVLAIIKNTP
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCC
PISQEALTSQAQSSVVYAKDENGNWQRVAILHGADNRLWVPIEKIPKNLQNAFVAIEDQR
CCCHHHHHHHHHCCEEEEECCCCCEEEEEEEECCCCEEEEEHHHHHHHHHHHEEEEECHH
FYKNKLGIDPKRIIGALIADIMAGGKPVEGASTITQQLVKNTMLSSEKTITRKIQEMVLA
HHHHHCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
WRLEQIYSKQQILEAYLNTIYLGGPNVNAYGVQAASLAYFGKDVSQLDLAECAMIAGITK
HHHHHHHHHHHHHHHHHHEEEECCCCCCEECCHHHHHHHHCCCHHHHHHHHHHHHHCCCC
NPSRYSPYADLEAATERQHLVLKKMLEQGYITKEEYEKAISEKLVFKKTNFTTYEHKYFI
CCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHEEEECCCCEEHHHHHH
DQVINDVADALSQHLNISHDEAIKKIYNGGLRIYSTMDLQIQKTMEEAFKNPKLFPPDIK
HHHHHHHHHHHHHHCCCCHHHHHHHHHCCCEEEEEEECHHHHHHHHHHHCCCCCCCCCCC
DRSGNTRIVQGAMVVMDWKTGEVKGIVGGRETEETKGKTRIFNYATQGGRPAGSSIKPLT
CCCCCEEEEEEEEEEEECCCCCEEEECCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCEE
VYGPALEKGYTAATVVDDVPVFYKQWNWEPHNYESGKYRGLMTFREALTYSENVPAARIV
EECCHHHCCCEEEEHHHCCCHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHH
VEMIGLHTAAEYGKKFGLTVTKDVASSPAALALGAGGAVTPLQEAAAYGAIANGGVYTTP
HHHHHHHHHHHHHHHCCCEEEHHHCCCCCEEEECCCCCCCHHHHHHHHCCCCCCCEEECC
ITFTKVTDSKGKVILENKPSQHMVLTPQNAYILTSMMQDVVKYGTGTNARLPNMPVAGKT
EEEEEEECCCCEEEEECCCCCCEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
GTNESYNDAWFTGFTPYYVGSVWMGTGENIPMIYGGRGVVGGSYPAMLWRTVMAEIHKSL
CCCCCCCCCEECCCCHHHHCEEEECCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHC
PYKDFERPPGIISVTVCKDSGELPTDLCHQDLRGDRTYTEIFAEGTQPTTYCTVHVLAKI
CCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHCCCCHHHHHHHCCCCCCEEEEEEEEEEE
NSQNGKLATEFTPPDLVKEAVFIDPPGRTPEQNAVALDGKYVVPKDYDDTPPPAQPPSGE
CCCCCEEEEECCCHHHHHHCEEECCCCCCCCCCEEEECCEEECCCCCCCCCCCCCCCCCC
VLPPENTQNPPSQPPNEIQQPPGNTPPNNGGNNGTTQNPPQNNNNNTSSNPLEKIFPPKN
CCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCC
SKP
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA