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The map label for this gene is PtsN4 [H]

Identifier: 20806823

GI number: 20806823

Start: 318321

End: 320402

Strand: Direct

Name: PtsN4 [H]

Synonym: TTE0303

Alternate gene names: 20806823

Gene position: 318321-320402 (Clockwise)

Preceding gene: 20806822

Following gene: 20806824

Centisome position: 11.84

GC content: 34.73

Gene sequence:

>2082_bases
TTGATGAAACTTATCAATTCAAACGAGCCAATAAAAATTTCTGAGCTTGCAAAATTATTCAATGTAAGCTCTAGAACTAT
AAGGTATGACCTCGATGCTATTGATGAGTTTTTAAAGTATAATAATTTGCCACAATTAATTAGAAAACCGAATGTAGGCG
TGAAATTTTCTGAACTATTAGAACATAGAAACAAAGCCTTATCCTTCTTAGACACTCTTTCTCCTTATTATTACAACTTA
TCCCAAAAAGAAAGAGTAAATGTAATACTAAGCGAGCTTATCCAACAGAGAGACTACATCACAATAAACACTTTAGCTGA
AAAGCTTATGGTTTCAAGAAGCACAGTCATAAGCGATTTAAAAAAAGTAAAAGAATGGTTAGAGGAAAGAGGGCTTTACC
TTAAAGCTTTACCCAAATACGGTGTAAAAGTCGTTGGAGATGAAAAGCAGTTAAGAAGAGCTGCTATTGAACTTCTTACA
GAAGCAATAGATATTGACAAAGCATTGGATATTGTAAAGGCACCTTTCTATGGGCGAAGTTTGGGCGGCAGTGGTCAGAT
TGCAAAGCTTTTTGAGGATATAGACATACCGTATATAGAGCAATGTGTGCAGATAGCAGAAAGAGAGCTGGAGACAATTT
TTTCCGATGCGGCTTTTTCTGGACTTGTGATTCACATAGCGATTGCAATAAAGAGAATTCAACTGGGCAAGGATATAGTA
ATGCCAAAAGAGGAATTAAAAGCTTTAGAAATGACTAAGGAATTCGCTGTTGCATCAAATATAGCTAAGATGTTAGAAGA
CCGCTTTAATGTATCAATACCTGTAGATGAAATTGGATACATCACTATCCATTTATTAGGAAGCAATGTAGCTAAGCCTA
AAACTTACCTCAATGAAAACTGGATTGAATATCAGCTTCTTACAGAAAAAATCATACGAAATGTTAGTGAAAGGATAAAA
GAAAACCTTTTAGAAGATCAGCAACTTTTTGAAGGTTTACTAGATCATTTGAGACCCACGATATACCGCTTGAAGCACGA
TTTAAAATTAAAAAATCCCATTCTAGATGAAATTAAAACTAATTACAGAGAACTTTTTGAAATAGTTAGGGAGAGCTTAA
AGCCTATTGAAGAGTACACAGGCAGGAACTTGAATGAAGAAGAAATAGGTTATTTTGTCATACATTTTGGTGCAGCAATA
GAAAGAAAAAAGACCGCTATTTCTATTAAGCCCAATGTATTAGTTGTATGTAGCACAGGAATAGGGACAGCAAAACTCCT
TTCTTCCAGATTGCAATCTGTTTTTGATGTACACATAATTGACACTATCGCTTTTCACCAGATTAAAGAGGTTTTAAAGG
ATAAAAAAATAGACTTGATAGTGTCTACTATTCCGCTCAAATGTGATGAAGTAAAAGTAGTCGAGGTCAATCCTCTTCTC
ACGGATAGGGATATAGAAAAATTAAGTAAATTCCTCGCTAAGCCTTTAGAAAGGCGCTTGGATGTGGTAGACGAGCTTAT
GGAAATCATAAATAGGCATTGCGTAATAAAAGATCGCGAAAAATTGCTAGAAGATTTACTAATTTTTTTCAACATAGCAT
CATATGAAAATAGGAGAGGAGTTGTTCATCCAGTGTTGAAGGACCTTTTGACAAAAGATACCATAAAGCTAAATGTTGAG
GCAAAAGATTGGGAAGAAGCTGTAAGGATAGGAGGTGAGTTGTTAGTCAAAAATGGTTTTGCTGAGCCTCAATACGTTGA
GGCAATGATAAACACTGTAAAAGAAATGGGACCTTACATTGTAATTGCACCAGGTATAGCAATGCCCCATGCAAGACCTG
AGGCAGGAGCAAGAAAAATAGGTATGAGTTTAATCACACTAAAAAATCCTGTTAACTTTGGGAACAAAGAAAATGATCCA
GTAAAAATTGTAGTTTCTCTCTGTGCAATTGATCATTCTTCACACCTTAAAGCATTATCTGAATTAGTGGAGCTTTTGGG
AGATGAGGAATTTGTAGAAGCAGTATTAAATGCAAATGATGTAGATAGAGTATTGAAATTATTAGGTAAAGAAAAAGTAT
AA

Upstream 100 bases:

>100_bases
TATTGAAAAATGTTAAGCTTTTCATTATCATAGAAGTATAATATAATTTACTTAAAAAGAGGTGATAATTATCACTCTCA
ATGACAGAAGTAAACAAATT

Downstream 100 bases:

>100_bases
ATCCATGTAGGAGGGTGGAATAATGTGGTTAATTGATTTTCTGGTAAAAAACATTTTTAATCAAGTAGCCGTTCTAATAG
GACTAGTTACACTTATAGGA

Product: phosphotransferase system mannitol/fructose-specific IIA domain-containing protein

Products: NA

Alternate protein names: Mannitol operon transcriptional activator; Mtl operon transcriptional activator; Putative phosphotransferase enzyme IIB component; Putative PTS system EIIB component; Putative phosphotransferase enzyme IIA component; Putative PTS system EIIA component [H]

Number of amino acids: Translated: 693; Mature: 693

Protein sequence:

>693_residues
MMKLINSNEPIKISELAKLFNVSSRTIRYDLDAIDEFLKYNNLPQLIRKPNVGVKFSELLEHRNKALSFLDTLSPYYYNL
SQKERVNVILSELIQQRDYITINTLAEKLMVSRSTVISDLKKVKEWLEERGLYLKALPKYGVKVVGDEKQLRRAAIELLT
EAIDIDKALDIVKAPFYGRSLGGSGQIAKLFEDIDIPYIEQCVQIAERELETIFSDAAFSGLVIHIAIAIKRIQLGKDIV
MPKEELKALEMTKEFAVASNIAKMLEDRFNVSIPVDEIGYITIHLLGSNVAKPKTYLNENWIEYQLLTEKIIRNVSERIK
ENLLEDQQLFEGLLDHLRPTIYRLKHDLKLKNPILDEIKTNYRELFEIVRESLKPIEEYTGRNLNEEEIGYFVIHFGAAI
ERKKTAISIKPNVLVVCSTGIGTAKLLSSRLQSVFDVHIIDTIAFHQIKEVLKDKKIDLIVSTIPLKCDEVKVVEVNPLL
TDRDIEKLSKFLAKPLERRLDVVDELMEIINRHCVIKDREKLLEDLLIFFNIASYENRRGVVHPVLKDLLTKDTIKLNVE
AKDWEEAVRIGGELLVKNGFAEPQYVEAMINTVKEMGPYIVIAPGIAMPHARPEAGARKIGMSLITLKNPVNFGNKENDP
VKIVVSLCAIDHSSHLKALSELVELLGDEEFVEAVLNANDVDRVLKLLGKEKV

Sequences:

>Translated_693_residues
MMKLINSNEPIKISELAKLFNVSSRTIRYDLDAIDEFLKYNNLPQLIRKPNVGVKFSELLEHRNKALSFLDTLSPYYYNL
SQKERVNVILSELIQQRDYITINTLAEKLMVSRSTVISDLKKVKEWLEERGLYLKALPKYGVKVVGDEKQLRRAAIELLT
EAIDIDKALDIVKAPFYGRSLGGSGQIAKLFEDIDIPYIEQCVQIAERELETIFSDAAFSGLVIHIAIAIKRIQLGKDIV
MPKEELKALEMTKEFAVASNIAKMLEDRFNVSIPVDEIGYITIHLLGSNVAKPKTYLNENWIEYQLLTEKIIRNVSERIK
ENLLEDQQLFEGLLDHLRPTIYRLKHDLKLKNPILDEIKTNYRELFEIVRESLKPIEEYTGRNLNEEEIGYFVIHFGAAI
ERKKTAISIKPNVLVVCSTGIGTAKLLSSRLQSVFDVHIIDTIAFHQIKEVLKDKKIDLIVSTIPLKCDEVKVVEVNPLL
TDRDIEKLSKFLAKPLERRLDVVDELMEIINRHCVIKDREKLLEDLLIFFNIASYENRRGVVHPVLKDLLTKDTIKLNVE
AKDWEEAVRIGGELLVKNGFAEPQYVEAMINTVKEMGPYIVIAPGIAMPHARPEAGARKIGMSLITLKNPVNFGNKENDP
VKIVVSLCAIDHSSHLKALSELVELLGDEEFVEAVLNANDVDRVLKLLGKEKV
>Mature_693_residues
MMKLINSNEPIKISELAKLFNVSSRTIRYDLDAIDEFLKYNNLPQLIRKPNVGVKFSELLEHRNKALSFLDTLSPYYYNL
SQKERVNVILSELIQQRDYITINTLAEKLMVSRSTVISDLKKVKEWLEERGLYLKALPKYGVKVVGDEKQLRRAAIELLT
EAIDIDKALDIVKAPFYGRSLGGSGQIAKLFEDIDIPYIEQCVQIAERELETIFSDAAFSGLVIHIAIAIKRIQLGKDIV
MPKEELKALEMTKEFAVASNIAKMLEDRFNVSIPVDEIGYITIHLLGSNVAKPKTYLNENWIEYQLLTEKIIRNVSERIK
ENLLEDQQLFEGLLDHLRPTIYRLKHDLKLKNPILDEIKTNYRELFEIVRESLKPIEEYTGRNLNEEEIGYFVIHFGAAI
ERKKTAISIKPNVLVVCSTGIGTAKLLSSRLQSVFDVHIIDTIAFHQIKEVLKDKKIDLIVSTIPLKCDEVKVVEVNPLL
TDRDIEKLSKFLAKPLERRLDVVDELMEIINRHCVIKDREKLLEDLLIFFNIASYENRRGVVHPVLKDLLTKDTIKLNVE
AKDWEEAVRIGGELLVKNGFAEPQYVEAMINTVKEMGPYIVIAPGIAMPHARPEAGARKIGMSLITLKNPVNFGNKENDP
VKIVVSLCAIDHSSHLKALSELVELLGDEEFVEAVLNANDVDRVLKLLGKEKV

Specific function: Positively regulates the expression of the mtlAFD operon involved in the uptake and catabolism of mannitol [H]

COG id: COG3711

COG function: function code K; Transcriptional antiterminator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIB type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI2367359, Length=127, Percent_Identity=46.4566929133858, Blast_Score=129, Evalue=4e-31,
Organism=Escherichia coli, GI1790755, Length=129, Percent_Identity=40.3100775193798, Blast_Score=107, Evalue=3e-24,
Organism=Escherichia coli, GI1789302, Length=115, Percent_Identity=40.8695652173913, Blast_Score=93, Evalue=5e-20,
Organism=Escherichia coli, GI1790027, Length=145, Percent_Identity=34.4827586206897, Blast_Score=79, Evalue=8e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013196
- InterPro:   IPR007737
- InterPro:   IPR011608
- InterPro:   IPR016152
- InterPro:   IPR002178
- InterPro:   IPR013011
- InterPro:   IPR003501
- InterPro:   IPR011991 [H]

Pfam domain/function: PF08279 HTH_11; PF05043 Mga; PF00874 PRD; PF02302 PTS_IIB [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 78976; Mature: 78976

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: PS51000 HTH_DEOR_2 ; PS00372 PTS_EIIA_TYPE_2_HIS ; PS51094 PTS_EIIA_TYPE_2 ; PS51099 PTS_EIIB_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMKLINSNEPIKISELAKLFNVSSRTIRYDLDAIDEFLKYNNLPQLIRKPNVGVKFSELL
CCCCCCCCCCEEHHHHHHHHCCCCCCEEEEHHHHHHHHHHCCCHHHHHCCCCCCCHHHHH
EHRNKALSFLDTLSPYYYNLSQKERVNVILSELIQQRDYITINTLAEKLMVSRSTVISDL
HHHHHHHHHHHHCCHHHCCCCHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHH
KKVKEWLEERGLYLKALPKYGVKVVGDEKQLRRAAIELLTEAIDIDKALDIVKAPFYGRS
HHHHHHHHHCCCEEEECCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
LGGSGQIAKLFEDIDIPYIEQCVQIAERELETIFSDAAFSGLVIHIAIAIKRIQLGKDIV
CCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
MPKEELKALEMTKEFAVASNIAKMLEDRFNVSIPVDEIGYITIHLLGSNVAKPKTYLNEN
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCEEEEEEECCCCCCCHHHHCCC
WIEYQLLTEKIIRNVSERIKENLLEDQQLFEGLLDHLRPTIYRLKHDLKLKNPILDEIKT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
NYRELFEIVRESLKPIEEYTGRNLNEEEIGYFVIHFGAAIERKKTAISIKPNVLVVCSTG
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHCEEEEEECHHHHHCCCEEEECCCEEEEEECC
IGTAKLLSSRLQSVFDVHIIDTIAFHQIKEVLKDKKIDLIVSTIPLKCDEVKVVEVNPLL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCEEEEEECCCC
TDRDIEKLSKFLAKPLERRLDVVDELMEIINRHCVIKDREKLLEDLLIFFNIASYENRRG
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCC
VVHPVLKDLLTKDTIKLNVEAKDWEEAVRIGGELLVKNGFAEPQYVEAMINTVKEMGPYI
CHHHHHHHHHCCCCEEEEEEHHHHHHHHHCCCHHEEECCCCCHHHHHHHHHHHHHHCCEE
VIAPGIAMPHARPEAGARKIGMSLITLKNPVNFGNKENDPVKIVVSLCAIDHSSHLKALS
EEECCCCCCCCCCCCHHHHHCHHHEEEECCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHH
ELVELLGDEEFVEAVLNANDVDRVLKLLGKEKV
HHHHHHCCHHHHHHHHCCCCHHHHHHHHCCCCC
>Mature Secondary Structure
MMKLINSNEPIKISELAKLFNVSSRTIRYDLDAIDEFLKYNNLPQLIRKPNVGVKFSELL
CCCCCCCCCCEEHHHHHHHHCCCCCCEEEEHHHHHHHHHHCCCHHHHHCCCCCCCHHHHH
EHRNKALSFLDTLSPYYYNLSQKERVNVILSELIQQRDYITINTLAEKLMVSRSTVISDL
HHHHHHHHHHHHCCHHHCCCCHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHH
KKVKEWLEERGLYLKALPKYGVKVVGDEKQLRRAAIELLTEAIDIDKALDIVKAPFYGRS
HHHHHHHHHCCCEEEECCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
LGGSGQIAKLFEDIDIPYIEQCVQIAERELETIFSDAAFSGLVIHIAIAIKRIQLGKDIV
CCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
MPKEELKALEMTKEFAVASNIAKMLEDRFNVSIPVDEIGYITIHLLGSNVAKPKTYLNEN
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCEEEEEEECCCCCCCHHHHCCC
WIEYQLLTEKIIRNVSERIKENLLEDQQLFEGLLDHLRPTIYRLKHDLKLKNPILDEIKT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
NYRELFEIVRESLKPIEEYTGRNLNEEEIGYFVIHFGAAIERKKTAISIKPNVLVVCSTG
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHCEEEEEECHHHHHCCCEEEECCCEEEEEECC
IGTAKLLSSRLQSVFDVHIIDTIAFHQIKEVLKDKKIDLIVSTIPLKCDEVKVVEVNPLL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCEEEEEECCCC
TDRDIEKLSKFLAKPLERRLDVVDELMEIINRHCVIKDREKLLEDLLIFFNIASYENRRG
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCC
VVHPVLKDLLTKDTIKLNVEAKDWEEAVRIGGELLVKNGFAEPQYVEAMINTVKEMGPYI
CHHHHHHHHHCCCCEEEEEEHHHHHHHHHCCCHHEEECCCCCHHHHHHHHHHHHHHCCEE
VIAPGIAMPHARPEAGARKIGMSLITLKNPVNFGNKENDPVKIVVSLCAIDHSSHLKALS
EEECCCCCCCCCCCCHHHHHCHHHEEEECCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHH
ELVELLGDEEFVEAVLNANDVDRVLKLLGKEKV
HHHHHHCCHHHHHHHHCCCCHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]