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The map label for this gene is RbsB [H]

Identifier: 20806732

GI number: 20806732

Start: 212410

End: 213351

Strand: Direct

Name: RbsB [H]

Synonym: TTE0206

Alternate gene names: 20806732

Gene position: 212410-213351 (Clockwise)

Preceding gene: 20806731

Following gene: 20806733

Centisome position: 7.9

GC content: 36.31

Gene sequence:

>942_bases
ATGAGAAAATCACGAATACTGTTACTTTTAACTATTTTTGTGACTTCAGCGGCGCTGATTTTGAGCGGTTGTAAAACAAA
TACTCCAAATACAGCTTCTACTAGTACCAAAGAGGGGAAAACTATAGGATTAGTGATATCTACTCTTAACAATCCATTCT
TTGTGACTTTAAAAAATGGTGCAGAAGAAAAAGCGAAAGAACTAGGATACAAAATAATAGTTGAAGATTCGCAAAATGAT
TCATCTAAAGAGCTTTCTAATGTAGAAGATTTGATTCAACAGAAAGTAGATGTTTTACTTATAAATCCTGTGGATAGCGA
TGCAGTTGTTACTGCTATAAAAGAAGCGAATAGCAAAAATATACCAGTTATAACTATAGATAGATCAGCAAATGGTGGAG
ATGTTGTTTGCCATATAGCTTCAGATAATGTTAAGGGTGGAGAAATGGCTGCAGAATTTATAGCTAAAGCTCTAAAAGGC
AAGGGGAATGTAGTAGAACTTGAAGGTATACCTGGGGCTTCTGCAGCAAGAGATAGAGGCAAAGGGTTTGATGAAGCTAT
TGCTAAGTATCCAGATATAAAAATAGTTGCAAAGCAGGCTGCTGATTTTGATCGTTCCAAAGGTTTATCAGTAATGGAAA
ACATCTTGCAAGCCCAGCCCAAAATTGATGCAGTATTTGCGCAAAATGATGAAATGGCTTTAGGAGCTATAAAAGCAATT
GAGGCAGCTAATAGACAAGGAATTATTGTTGTGGGATTTGATGGGACAGAAGATGCTCTAAAAGCTATAAAAGAAGGGAA
AATGGCTGCTACAATTGCGCAGCAGCCAGCTTTAATGGGATCACTTGGTGTGGAGATGGCTGATAAATACCTAAAAGGTG
AAAAAATACCTAACTTTATTCCTGCAGAACTTAAACTCATAACAAAAGAAAATGTACAGTGA

Upstream 100 bases:

>100_bases
AAAGTGAGTAAATGAGAAAAGGGGGGATGTTTGGAAATTTAGAGTTTTTATTAGAGTTTTGGATAGTTTATATTAAAAAA
TTAAAGGGGTGGAGAGATTT

Downstream 100 bases:

>100_bases
TTTGCGATTTTTGCCTGTCACTAAAGTGACAGGCGTTTTTCTTTTTAACTTTATTGTAATTATTGGGAAATATATTTGTC
AAGTCTTTAATGATTCAATA

Product: periplasmic sugar-binding proteins

Products: ADP; phosphate; ribose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MRKSRILLLLTIFVTSAALILSGCKTNTPNTASTSTKEGKTIGLVISTLNNPFFVTLKNGAEEKAKELGYKIIVEDSQND
SSKELSNVEDLIQQKVDVLLINPVDSDAVVTAIKEANSKNIPVITIDRSANGGDVVCHIASDNVKGGEMAAEFIAKALKG
KGNVVELEGIPGASAARDRGKGFDEAIAKYPDIKIVAKQAADFDRSKGLSVMENILQAQPKIDAVFAQNDEMALGAIKAI
EAANRQGIIVVGFDGTEDALKAIKEGKMAATIAQQPALMGSLGVEMADKYLKGEKIPNFIPAELKLITKENVQ

Sequences:

>Translated_313_residues
MRKSRILLLLTIFVTSAALILSGCKTNTPNTASTSTKEGKTIGLVISTLNNPFFVTLKNGAEEKAKELGYKIIVEDSQND
SSKELSNVEDLIQQKVDVLLINPVDSDAVVTAIKEANSKNIPVITIDRSANGGDVVCHIASDNVKGGEMAAEFIAKALKG
KGNVVELEGIPGASAARDRGKGFDEAIAKYPDIKIVAKQAADFDRSKGLSVMENILQAQPKIDAVFAQNDEMALGAIKAI
EAANRQGIIVVGFDGTEDALKAIKEGKMAATIAQQPALMGSLGVEMADKYLKGEKIPNFIPAELKLITKENVQ
>Mature_313_residues
MRKSRILLLLTIFVTSAALILSGCKTNTPNTASTSTKEGKTIGLVISTLNNPFFVTLKNGAEEKAKELGYKIIVEDSQND
SSKELSNVEDLIQQKVDVLLINPVDSDAVVTAIKEANSKNIPVITIDRSANGGDVVCHIASDNVKGGEMAAEFIAKALKG
KGNVVELEGIPGASAARDRGKGFDEAIAKYPDIKIVAKQAADFDRSKGLSVMENILQAQPKIDAVFAQNDEMALGAIKAI
EAANRQGIIVVGFDGTEDALKAIKEGKMAATIAQQPALMGSLGVEMADKYLKGEKIPNFIPAELKLITKENVQ

Specific function: Involved in the high-affinity D-ribose membrane transport system and also serves as the primary chemoreceptor for chemotaxis [H]

COG id: COG1879

COG function: function code G; ABC-type sugar transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 2 family [H]

Homologues:

Organism=Escherichia coli, GI1790192, Length=280, Percent_Identity=56.0714285714286, Blast_Score=314, Evalue=4e-87,
Organism=Escherichia coli, GI1790526, Length=287, Percent_Identity=35.8885017421603, Blast_Score=152, Evalue=2e-38,
Organism=Escherichia coli, GI1790674, Length=249, Percent_Identity=36.144578313253, Blast_Score=135, Evalue=3e-33,
Organism=Escherichia coli, GI1789990, Length=252, Percent_Identity=30.952380952381, Blast_Score=95, Evalue=5e-21,
Organism=Escherichia coli, GI1790194, Length=238, Percent_Identity=27.7310924369748, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1788473, Length=253, Percent_Identity=28.8537549407115, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1787948, Length=299, Percent_Identity=25.0836120401338, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1786268, Length=200, Percent_Identity=25, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1788898, Length=272, Percent_Identity=27.5735294117647, Blast_Score=69, Evalue=5e-13,

Paralogues:

None

Copy number: 3940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5900 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 1520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001761 [H]

Pfam domain/function: PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 33281; Mature: 33281

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKSRILLLLTIFVTSAALILSGCKTNTPNTASTSTKEGKTIGLVISTLNNPFFVTLKNG
CCCCCEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEEECCCCEEEEECCC
AEEKAKELGYKIIVEDSQNDSSKELSNVEDLIQQKVDVLLINPVDSDAVVTAIKEANSKN
CHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHCCCCC
IPVITIDRSANGGDVVCHIASDNVKGGEMAAEFIAKALKGKGNVVELEGIPGASAARDRG
CCEEEEECCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHCC
KGFDEAIAKYPDIKIVAKQAADFDRSKGLSVMENILQAQPKIDAVFAQNDEMALGAIKAI
CCHHHHHHHCCCEEEEEHHHCCCCHHCCHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHH
EAANRQGIIVVGFDGTEDALKAIKEGKMAATIAQQPALMGSLGVEMADKYLKGEKIPNFI
HHCCCCCEEEEECCCCHHHHHHHHCCCHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCC
PAELKLITKENVQ
CCCEEEEECCCCC
>Mature Secondary Structure
MRKSRILLLLTIFVTSAALILSGCKTNTPNTASTSTKEGKTIGLVISTLNNPFFVTLKNG
CCCCCEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEEECCCCEEEEECCC
AEEKAKELGYKIIVEDSQNDSSKELSNVEDLIQQKVDVLLINPVDSDAVVTAIKEANSKN
CHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHCCCCC
IPVITIDRSANGGDVVCHIASDNVKGGEMAAEFIAKALKGKGNVVELEGIPGASAARDRG
CCEEEEECCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHCC
KGFDEAIAKYPDIKIVAKQAADFDRSKGLSVMENILQAQPKIDAVFAQNDEMALGAIKAI
CCHHHHHHHCCCEEEEEHHHCCCCHHCCHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHH
EAANRQGIIVVGFDGTEDALKAIKEGKMAATIAQQPALMGSLGVEMADKYLKGEKIPNFI
HHCCCCCEEEEECCCCHHHHHHHHCCCHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCC
PAELKLITKENVQ
CCCEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; ribose [Periplasm]; H2O [C]

Specific reaction: ATP + ribose [Periplasm] + H2O = ADP + phosphate + ribose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800; 10675023 [H]