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The map label for this gene is HypF [H]

Identifier: 20806660

GI number: 20806660

Start: 130927

End: 133182

Strand: Direct

Name: HypF [H]

Synonym: TTE0131

Alternate gene names: 20806660

Gene position: 130927-133182 (Clockwise)

Preceding gene: 20806659

Following gene: 20806661

Centisome position: 4.87

GC content: 38.61

Gene sequence:

>2256_bases
ATGGTTAGAGGAAGAGTACCACAAATTCAGGCAAGGCAAATAAATATCTTTGGAATTGTACAGGGCGTGGGTTTTCGCCC
TTTTGTCTTTAATATTGCCCAGAAATACAATTTAAAAGGAATAGTTTACAACAACTCCTCAGGTCTATATATAGAAGTAG
AAGGAGAAGAAAAGGACATAGAAGCTTTTATAAGAGAGATAAAGGAAAATCCTCCCTCTCTTTCAGTAATTGATGAAATA
CAGGTAAGAGAAGTAGAAGTAAAAGAATACAAAGACTTTAAAATTGTGGGAAGCAAAGAGGATGGAGGATTTGTGCCTGT
ATCTCCTGATATGGGGGTCTGCGAAGACTGCCTAAGGGAGTTAAAAGACCCGAAAGACAGAAGGTACAGATATCCTTTTA
TAAACTGCACCAACTGCGGGCCTAGATTTTCTATAATTGAAGACATTCCTTATGATAGGGCAAAGACTTCTATGAAGGTT
TTCCCGATGTGCGAAAAGTGTAGCAGAGAATACCATGACCCCCATGACAGGAGGTTTCATGCTCAGCCTGTTGCATGTTT
TGACTGTGGTCCTTCTCTTTCCTTTGTTGGCGAAGGATGCTTTGATGATGAGATAAAATGTGTTGCAAAGGCTTTAAAAG
AAGGGAAAATTGTTGCGATTAAAGGCATTGGAGGCTTTCACTTGGCAGTAAATGCCCTGGACGATGAGGCAGTTGCCACT
TTAAGGAGACGCAAAAAAAGGTATGGCAAGCCTTTTGCTGTTATGATGAGAGATGTTGAAGAAGTGAAAAAGTACTGCAT
AGTTTCCCCTGAAGAAGAGAGGCTTCTTTTGAGTCAGAGAAGGCCTATTGTGCTTTTAAAGAAAAAGGGAGAAAAGCTTG
CAAAAGGGATAGCGGATGACTTGGATACTTTAGGAGTTATGCTTCCCTATGCTCCTATTCACTACCTTTTGATGGAGGAG
ATTGACTTTCCTATTGTGATGACGAGTGGAAATGTAAGTGAAGAGCCTATATGCAAGGACAATGAAGAGGCCTTAGAAAA
GCTTAAAGATATTGCTGATGTGTTTCTTTTAAATAACAGGGATATAGTGAACAGGATAGACGATTCTGTTACTTCTTTTA
ATGCGGGCGCAGAGAGGATAATAAGAAGGGCGAGGGGATATGCGCCTCAACCGATTCTGTTAAAAAAAGAGGTAAAAGCT
AGTATACTGGCTGTTGGAGGCTTTTACAAAAACACTTTTTGCATGACAAAAGGGCATTACGCCTTTATAAGCCATCACAT
TGGAGATTTGGACAATGAAAAGGCTTTTAATTATTACATTGAGCAAATAGAGAGGTATAAAAAGCTTTTTAGAGTAGACC
CCGAGGTAGTAGCCCATGATATGCACAAAGGATATTTGTCGACGCAGTATGCTAAATCTCTTGATTTGCCTAAAATTGAA
GTTCAGCATCACCATGCTCACATTGCCAGTTGCATGGCAGAGCACAATTTGGATGAAAAAGTAATTGGGATAGCTTATGA
TGGCACAGGATACGGCACAGATGGGAATGTGTGGGGAGCTGAAATTTTGGTATGCGATTTAAAGAGCTTTGAGAGAATCG
CTCACTTAAAGTATAAACCACTTCCAGGGAATGAACTGGCAATAAAAAAGATATACAGAACAGCTTTAGGGTTCATTTTC
GACAATATTAGCTTTTACAAGAATTTTGTCGAACAAGTTGACTCAAGAGAACTTGACATAATTTTAAAGCAGATAGATAG
AAAAATAAACACAGCTTATGTTTCAAGCATGGGAAGGTTTTTTGATGCAGTAGCGGCACTGATTGGTGTGAGAAAAGAAG
TTCTATTTGAAGGGCAGGCGGCTATGGAGCTTGAGAGTTTAATGGCTGAAAGTGAGGAATATTACGAGTATGAAATTCTA
AAAGAAGATAGATATGTAATTGACCCTGAGCTTATTCTAAGGCAAATTTATGAAGACTACATGAAGGGCTTTGAAAAAAG
CTATATATCGGCTAAATTTCACAACACAGTTGTCAATTTTACTTATGATTTAGCCAATCTTATAAGAAAAGAAACAGGAA
TAAACAAAGTAGTGCTAAGTGGTGGAAGTTTTCAAAACAGGTACCTTTTGAGGAGGCTAATAGAAAAACTTTCACTATCA
GGTTTTGAAGTTTATTCAAACAGCAAAGTACCCTGCAATGACGGAGGAATTTCTTTAGGACAGGCGGTTATTGCCAATAA
AATACTGGAGGGATAA

Upstream 100 bases:

>100_bases
ACAGAAAGTTTTTCTATGATGGTGTTAGAGCATTAAATCAAGAAGCGCCAATTTTTGAAGTCTCTGCAAGAACAGGGGAA
GGATTTGGTGAATTTGCAAG

Downstream 100 bases:

>100_bases
AGGTGTGTATTGCAGTTCCATCAAAAGTTTTAAAAATTGAAGGGAAAATTGCAGAGACAGAATTGGGAGGAGTAATAAGA
AGGGTTTCTATCGAAATGGT

Product: hydrogenase maturation factor

Products: NA

Alternate protein names: Carbamoyl phosphate-converting enzyme hypF; [NiFe]-hydrogenase maturation factor hypF; Hydrogenase maturation protein hypF [H]

Number of amino acids: Translated: 751; Mature: 751

Protein sequence:

>751_residues
MVRGRVPQIQARQINIFGIVQGVGFRPFVFNIAQKYNLKGIVYNNSSGLYIEVEGEEKDIEAFIREIKENPPSLSVIDEI
QVREVEVKEYKDFKIVGSKEDGGFVPVSPDMGVCEDCLRELKDPKDRRYRYPFINCTNCGPRFSIIEDIPYDRAKTSMKV
FPMCEKCSREYHDPHDRRFHAQPVACFDCGPSLSFVGEGCFDDEIKCVAKALKEGKIVAIKGIGGFHLAVNALDDEAVAT
LRRRKKRYGKPFAVMMRDVEEVKKYCIVSPEEERLLLSQRRPIVLLKKKGEKLAKGIADDLDTLGVMLPYAPIHYLLMEE
IDFPIVMTSGNVSEEPICKDNEEALEKLKDIADVFLLNNRDIVNRIDDSVTSFNAGAERIIRRARGYAPQPILLKKEVKA
SILAVGGFYKNTFCMTKGHYAFISHHIGDLDNEKAFNYYIEQIERYKKLFRVDPEVVAHDMHKGYLSTQYAKSLDLPKIE
VQHHHAHIASCMAEHNLDEKVIGIAYDGTGYGTDGNVWGAEILVCDLKSFERIAHLKYKPLPGNELAIKKIYRTALGFIF
DNISFYKNFVEQVDSRELDIILKQIDRKINTAYVSSMGRFFDAVAALIGVRKEVLFEGQAAMELESLMAESEEYYEYEIL
KEDRYVIDPELILRQIYEDYMKGFEKSYISAKFHNTVVNFTYDLANLIRKETGINKVVLSGGSFQNRYLLRRLIEKLSLS
GFEVYSNSKVPCNDGGISLGQAVIANKILEG

Sequences:

>Translated_751_residues
MVRGRVPQIQARQINIFGIVQGVGFRPFVFNIAQKYNLKGIVYNNSSGLYIEVEGEEKDIEAFIREIKENPPSLSVIDEI
QVREVEVKEYKDFKIVGSKEDGGFVPVSPDMGVCEDCLRELKDPKDRRYRYPFINCTNCGPRFSIIEDIPYDRAKTSMKV
FPMCEKCSREYHDPHDRRFHAQPVACFDCGPSLSFVGEGCFDDEIKCVAKALKEGKIVAIKGIGGFHLAVNALDDEAVAT
LRRRKKRYGKPFAVMMRDVEEVKKYCIVSPEEERLLLSQRRPIVLLKKKGEKLAKGIADDLDTLGVMLPYAPIHYLLMEE
IDFPIVMTSGNVSEEPICKDNEEALEKLKDIADVFLLNNRDIVNRIDDSVTSFNAGAERIIRRARGYAPQPILLKKEVKA
SILAVGGFYKNTFCMTKGHYAFISHHIGDLDNEKAFNYYIEQIERYKKLFRVDPEVVAHDMHKGYLSTQYAKSLDLPKIE
VQHHHAHIASCMAEHNLDEKVIGIAYDGTGYGTDGNVWGAEILVCDLKSFERIAHLKYKPLPGNELAIKKIYRTALGFIF
DNISFYKNFVEQVDSRELDIILKQIDRKINTAYVSSMGRFFDAVAALIGVRKEVLFEGQAAMELESLMAESEEYYEYEIL
KEDRYVIDPELILRQIYEDYMKGFEKSYISAKFHNTVVNFTYDLANLIRKETGINKVVLSGGSFQNRYLLRRLIEKLSLS
GFEVYSNSKVPCNDGGISLGQAVIANKILEG
>Mature_751_residues
MVRGRVPQIQARQINIFGIVQGVGFRPFVFNIAQKYNLKGIVYNNSSGLYIEVEGEEKDIEAFIREIKENPPSLSVIDEI
QVREVEVKEYKDFKIVGSKEDGGFVPVSPDMGVCEDCLRELKDPKDRRYRYPFINCTNCGPRFSIIEDIPYDRAKTSMKV
FPMCEKCSREYHDPHDRRFHAQPVACFDCGPSLSFVGEGCFDDEIKCVAKALKEGKIVAIKGIGGFHLAVNALDDEAVAT
LRRRKKRYGKPFAVMMRDVEEVKKYCIVSPEEERLLLSQRRPIVLLKKKGEKLAKGIADDLDTLGVMLPYAPIHYLLMEE
IDFPIVMTSGNVSEEPICKDNEEALEKLKDIADVFLLNNRDIVNRIDDSVTSFNAGAERIIRRARGYAPQPILLKKEVKA
SILAVGGFYKNTFCMTKGHYAFISHHIGDLDNEKAFNYYIEQIERYKKLFRVDPEVVAHDMHKGYLSTQYAKSLDLPKIE
VQHHHAHIASCMAEHNLDEKVIGIAYDGTGYGTDGNVWGAEILVCDLKSFERIAHLKYKPLPGNELAIKKIYRTALGFIF
DNISFYKNFVEQVDSRELDIILKQIDRKINTAYVSSMGRFFDAVAALIGVRKEVLFEGQAAMELESLMAESEEYYEYEIL
KEDRYVIDPELILRQIYEDYMKGFEKSYISAKFHNTVVNFTYDLANLIRKETGINKVVLSGGSFQNRYLLRRLIEKLSLS
GFEVYSNSKVPCNDGGISLGQAVIANKILEG

Specific function: Along with hypE, it catalyzes the synthesis of the CN ligands of the active site iron of [NiFe]-hydrogenases using carbamoylphosphate as a substrate. It functions as a carbamoyl transferase using carbamoylphosphate as a substrate and transferring the carb

COG id: COG0068

COG function: function code O; Hydrogenase maturation factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 YrdC-like domain [H]

Homologues:

Organism=Escherichia coli, GI2367152, Length=752, Percent_Identity=40.2925531914894, Blast_Score=486, Evalue=1e-138,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001792
- InterPro:   IPR017968
- InterPro:   IPR004421
- InterPro:   IPR017945
- InterPro:   IPR006070
- InterPro:   IPR011125 [H]

Pfam domain/function: PF00708 Acylphosphatase; PF01300 Sua5_yciO_yrdC; PF07503 zf-HYPF [H]

EC number: NA

Molecular weight: Translated: 85548; Mature: 85548

Theoretical pI: Translated: 6.56; Mature: 6.56

Prosite motif: PS00150 ACYLPHOSPHATASE_1 ; PS51160 ACYLPHOSPHATASE_3 ; PS51163 YRDC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVRGRVPQIQARQINIFGIVQGVGFRPFVFNIAQKYNLKGIVYNNSSGLYIEVEGEEKDI
CCCCCCCCCCCEEEEEEEEEECCCCCHHHHHHHHHCCCEEEEEECCCCEEEEECCCHHHH
EAFIREIKENPPSLSVIDEIQVREVEVKEYKDFKIVGSKEDGGFVPVSPDMGVCEDCLRE
HHHHHHHHCCCCCEEHCCHHHHEEEEHHCCCCEEEEECCCCCCEEECCCCCCHHHHHHHH
LKDPKDRRYRYPFINCTNCGPRFSIIEDIPYDRAKTSMKVFPMCEKCSREYHDPHDRRFH
HCCCHHCCEECCEEEECCCCCCCHHHHCCCCCHHHCCEEECHHHHHHHHHCCCCCCCCCC
AQPVACFDCGPSLSFVGEGCFDDEIKCVAKALKEGKIVAIKGIGGFHLAVNALDDEAVAT
CCCEEEEECCCCHHHCCCCCCCHHHHHHHHHHHCCCEEEEECCCCEEEEEECCCHHHHHH
LRRRKKRYGKPFAVMMRDVEEVKKYCIVSPEEERLLLSQRRPIVLLKKKGEKLAKGIADD
HHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCEEEEECCCHHHHHHHHHH
LDTLGVMLPYAPIHYLLMEEIDFPIVMTSGNVSEEPICKDNEEALEKLKDIADVFLLNNR
HHHHHHHHCHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHEECCC
DIVNRIDDSVTSFNAGAERIIRRARGYAPQPILLKKEVKASILAVGGFYKNTFCMTKGHY
HHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCEEEHHHHHHHHEEECCHHHCEEEEECCCH
AFISHHIGDLDNEKAFNYYIEQIERYKKLFRVDPEVVAHDMHKGYLSTQYAKSLDLPKIE
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCEEE
VQHHHAHIASCMAEHNLDEKVIGIAYDGTGYGTDGNVWGAEILVCDLKSFERIAHLKYKP
HHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCEEEEEEEHHHHHHHHHCCCCC
LPGNELAIKKIYRTALGFIFDNISFYKNFVEQVDSRELDIILKQIDRKINTAYVSSMGRF
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
FDAVAALIGVRKEVLFEGQAAMELESLMAESEEYYEYEILKEDRYVIDPELILRQIYEDY
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHEEEEECCCCEEECHHHHHHHHHHHH
MKGFEKSYISAKFHNTVVNFTYDLANLIRKETGINKVVLSGGSFQNRYLLRRLIEKLSLS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHCCC
GFEVYSNSKVPCNDGGISLGQAVIANKILEG
CEEEECCCCCCCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MVRGRVPQIQARQINIFGIVQGVGFRPFVFNIAQKYNLKGIVYNNSSGLYIEVEGEEKDI
CCCCCCCCCCCEEEEEEEEEECCCCCHHHHHHHHHCCCEEEEEECCCCEEEEECCCHHHH
EAFIREIKENPPSLSVIDEIQVREVEVKEYKDFKIVGSKEDGGFVPVSPDMGVCEDCLRE
HHHHHHHHCCCCCEEHCCHHHHEEEEHHCCCCEEEEECCCCCCEEECCCCCCHHHHHHHH
LKDPKDRRYRYPFINCTNCGPRFSIIEDIPYDRAKTSMKVFPMCEKCSREYHDPHDRRFH
HCCCHHCCEECCEEEECCCCCCCHHHHCCCCCHHHCCEEECHHHHHHHHHCCCCCCCCCC
AQPVACFDCGPSLSFVGEGCFDDEIKCVAKALKEGKIVAIKGIGGFHLAVNALDDEAVAT
CCCEEEEECCCCHHHCCCCCCCHHHHHHHHHHHCCCEEEEECCCCEEEEEECCCHHHHHH
LRRRKKRYGKPFAVMMRDVEEVKKYCIVSPEEERLLLSQRRPIVLLKKKGEKLAKGIADD
HHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCEEEEECCCHHHHHHHHHH
LDTLGVMLPYAPIHYLLMEEIDFPIVMTSGNVSEEPICKDNEEALEKLKDIADVFLLNNR
HHHHHHHHCHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHEECCC
DIVNRIDDSVTSFNAGAERIIRRARGYAPQPILLKKEVKASILAVGGFYKNTFCMTKGHY
HHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCEEEHHHHHHHHEEECCHHHCEEEEECCCH
AFISHHIGDLDNEKAFNYYIEQIERYKKLFRVDPEVVAHDMHKGYLSTQYAKSLDLPKIE
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCEEE
VQHHHAHIASCMAEHNLDEKVIGIAYDGTGYGTDGNVWGAEILVCDLKSFERIAHLKYKP
HHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCEEEEEEEHHHHHHHHHCCCCC
LPGNELAIKKIYRTALGFIFDNISFYKNFVEQVDSRELDIILKQIDRKINTAYVSSMGRF
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
FDAVAALIGVRKEVLFEGQAAMELESLMAESEEYYEYEILKEDRYVIDPELILRQIYEDY
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHEEEEECCCCEEECHHHHHHHHHHHH
MKGFEKSYISAKFHNTVVNFTYDLANLIRKETGINKVVLSGGSFQNRYLLRRLIEKLSLS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHCCC
GFEVYSNSKVPCNDGGISLGQAVIANKILEG
CEEEECCCCCCCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]